Abstract：
:Neurons are distinctive in that they are generally considered to be permanently post-mitotic cells. The oncoprotein p53 is a key regulator in neuronal development, notably in cell proliferation and neuronal death. We hypothesize that p53 maintains the post-mitotic characteristic of differentiated neurons. New lines of conditionally immortalized cortical cells were generated to test this hypothesis. Populations of cells were obtained from the neocortices of dual transgenic mice that were null for p53 and expressed a temperature-sensitive SV40 large T antigen. At a permissive temperature (32 degrees C), the cells continued to proliferate and most expressed nestin and proteins associated with glia. At a non-permissive temperature (39 degrees C), the cells expressed cytoskeletal proteins associated with differentiated neurons such as microtubule associated protein 2 and neurofilament 200. Under permissive conditions, both p53(+/-) and p53(-/-) cells exhibited similar cycling behaviors; the length of the cell cycle was 13-15 h and >85% of the cells were actively cycling. In non-permissive conditions, most p53(+/-) cells stopped dividing, whereas the p53(-/-) cells continued to proliferate. The survival of the cells also differed. In the non-permissive conditions, many p53(+/-) cells died following treatment with a neurotoxin (ethanol, 400 mg/dl), whereas the p53(-/-) cells did not. After re-introduction to the permissive conditions, both cell lines expressed neuron-like characteristics, but only the p53(-/-) cells retained their ability to cycle. Therefore, p53-mediated activities appear to be involved in the proliferation, survival, and post-mitotic nature of neuron-like cells.
journal_name
Brain Resjournal_title
Brain researchauthors
Miller MW,Peter A,Wharton SB,Wyllie AHdoi
10.1016/s0006-8993(02)04119-7subject
Has Abstractpub_date
2003-03-07 00:00:00pages
57-66issue
1-2eissn
0006-8993issn
1872-6240pii
S0006899302041197journal_volume
965pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:When rat striatal membranes, photolabeled with [3H]dopamine under assay conditions similar to those used for dopamine-sensitive adenylate cyclase, were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, several radioactively labeled bands appeared. Labeling of these bands was reduced in the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90426-1
更新日期：1981-12-28 00:00:00
abstract：:Analysis of rat hypothalamic extracts by gel filtration and HPLC revealed the presence of a form of substance P-like immunoreactivity with a molecular weight of approximately 5,700 (6k-SP). 6k-SP is probably an N-terminally extended form of substance P; the peptide may be an intermediate in SP biosynthesis, or may fun...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90310-x
更新日期：1984-12-10 00:00:00
abstract：:Retrograde axonal transport method of the fluorescent tracer FluoroGold (FG) was combined with immunocytochemistry to investigate the occurrence of nitric oxide synthase (NOS), somatostatin (SOM), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) in both intrinsic and cortically projecting neurons of the ra...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02408-8
更新日期：2001-06-29 00:00:00
abstract：:Previous studies have shown that preganglionic nerve stimulation in vitro increases acutely the activity of tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, in sympathetic neuronal cell bodies in the rat superior cervical ganglion (SCG). In the present study, we have examined whether ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90506-7
更新日期：1990-07-30 00:00:00
abstract：:The principal thalamic and hypothalamic structures implicated in mnemonic information processing are the mediodorsal nucleus of the thalamus, the pulvinar, anterior thalamus, and laterodorsal nucleus, the mamillary body, and the mamillothalamic tract and internal medullary lamina. Determining the contribution of an in...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/0006-8993(88)91226-7
更新日期：1988-12-01 00:00:00
abstract：:In acute cats deeply anesthetized with Nembutal, monosynaptic excitatory postsynaptic potentials (EPSPs) triggered by stimulation of the ventrolateral (VL) thalamic nucleus and the pes pedunculus were recorded in large pyramidal tract cells (PT cells). Deep anesthesia, low intensities of stimulation and an averaging t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90598-5
更新日期：1979-01-05 00:00:00
abstract：:The regional distribution of proline biosynthetic enzymes, ornithine-delta-aminotransferase and delta 1-pyrroline-5-carboxylate reductase, in the rat brain, and basic conditions for proline synthesis from ornithine in rat and chicken brain slices and chicken retinas were investigated. The cerebral regions relating to ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91022-5
更新日期：1987-06-16 00:00:00
abstract：:To test the hypothesis that presence of metalloproteases (MMPs), their inhibitors (TIMPs) and their substrate laminin-5 differs between the ischemic core and the surrounding tissue, we examined the impact of middle cerebral artery occlusion/reperfusion (MCA:O/R) on these molecules in different regions of the infarct. ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)03088-9
更新日期：2003-09-12 00:00:00
abstract：:Quantitative [125I]protein G-based immunohistochemistry was used to map the distribution of beta1 thyroid hormone receptor (TRbeta1) in normal and thyroidectomized adult rat brain, using a previously characterized polyclonal antibody. The distribution of TRbeta1-like immunoreactivity in normal brain was largely but no...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00450-2
更新日期：1997-06-27 00:00:00
abstract：:The nicotine and muscarinic responses of differentiated mouse neuroblastoma cells from the clonal line N1E 115 to applied cholinergic agents were recorded using single channel and whole cell patch clamp techniques. An inward macroscopic current induced by acetylcholine (ACh) at the resting potential was blocked by cur...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91252-7
更新日期：1986-11-19 00:00:00
abstract：:In the context of injury to the corticospinal tract (CST), brainstem-origin circuits may provide an alternative system of descending motor influence. However, subcortical circuits are largely under subconscious control. To improve volitional control over spared fibers after CST injury, we hypothesized that a combinati...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2013.07.012
更新日期：2013-11-13 00:00:00
abstract：:The localization of the methionine-enkephalin-Arg6-Gly7-Leu8 (Met-Enk-Arg-Gly-Leu)-like immunoreactivity-containing neurons in the rat lumbosacral spinal cord was immunohistochemically examined by an antiserum very specific to Met-Enk-Arg-Gly-Leu. The immunoreactive neurons occupied the positions corresponding to the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90804-3
更新日期：1987-03-17 00:00:00
abstract：:Our previous work has demonstrated that 100-Hz electroacupuncture (EA) or 100-Hz transcutaneous electrical nerve stimulation (TENS) was very effective in ameliorating the morphine withdrawal syndrome in rats and humans. The mechanism was obscure. (1) Rats were made dependent on morphine by repeated morphine injections...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)02069-7
更新日期：1999-12-18 00:00:00
abstract：:Quantitative autoradiographic analysis was used to identify regions in the brain of the male primate where androgen binding sites may be involved in the actions of testosterone. Three days after castration, adult male rhesus monkeys received a subcutaneous injection of either dihydrotestosterone propionate (DHTP, 20 m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90456-3
更新日期：1989-11-13 00:00:00
abstract：:A small, unilateral, penetrating wound was placed in the dorsolateral portion of the lumbosacral spinal cord in immature rats. Although this wound did not extend into the central canal area, some cells forming the walls of this canal underwent proliferative activity as demonstrated by [3H]thymidine autoradiography. Th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90783-0
更新日期：1980-11-10 00:00:00
abstract：:To determine if aging in rats is associated with insensitivity of cerebral tissue to thyroid hormones (TH), the expression of a TH responsive protein or (THRP) in cerebral tissue was studied in male Fischer rats at 4, 12 and 24 months of age during euthyroid, hypothyroid and hyperthyroid states. The basal levels of TH...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00144-9
更新日期：1998-05-18 00:00:00
abstract：:This study attempted to ascertain whether heatstroke-induced ischemia is associated with augmented striatal glutamate release and can be attenuated by NMDA receptor antagonists. Mean arterial pressure (MAP), striatal cerebral blood flow (CBF), striatal glutamate release and striatal neuronal damage score were assessed...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00282-0
更新日期：1998-06-08 00:00:00
abstract：:The purpose of this study was to identify the CNS cellular constituent immunoreactive for specific P2X7 receptor antiserum in the kainate-induced seizure and non-seizure rat brain. Analysis of P2X7 immunocytochemistry (ICC) revealed small immunoreactive cells with processes showing distinct morphological changes as se...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.03.040
更新日期：2006-05-17 00:00:00
abstract：:While much has been elucidated about the hypothalamic controls of energy balance, the epidemic of obesity continues to escalate. Recent work has suggested that extra-hypothalamic central nervous system structures may play a previously un-appreciated role in the control of ingestive behavior and body weight regulation....
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2010.06.009
更新日期：2010-09-02 00:00:00
abstract：:The effect of hypoxia on the structure and function of the synaptosomal membranes and myelin fraction (glial cells, neuronal cells bodies and axonal membranes) was investigated by measuring Na+,K(+)-ATPase activity and levels of lipid peroxidation products in cerebral cortical synaptosomal membranes and myelin fractio...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91388-9
更新日期：1993-01-15 00:00:00
abstract：:The effects of intracerebroventricular (i.c.v.) infusion of arginine vasopressin (AVP) on intracranial pressure (ICP), blood pressure (BP) and plasma AVP were investigated in conscious goats. The animals were implanted with ventricular (V) and cisternal (C) cannulae under halothane anaesthesia and allowed to recover p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90850-x
更新日期：1987-10-13 00:00:00
abstract：:The non-toxic neuronal binding domain of tetanus toxin (tetanus toxin fragment C, TTC) has been used as a vector to enhance delivery of potentially therapeutic proteins to motor neurons from the periphery following an intramuscular injection. The unique binding and transport properties of this 50-kDa polypeptide sugge...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.03.007
更新日期：2004-06-11 00:00:00
abstract：:Facilitation of cerebellorubral transmission was illustrated in nembutalized cats on paired stimulation of the cerebellar nucleus interpositus by an example of monosynaptic EPSPs of the rubrospinal neurons. It was found that the facilitation stated is not determined by the change in the presynaptic volley of impulses....
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90334-9
更新日期：1987-05-05 00:00:00
abstract：:To investigate the role of the estrogen receptor (ER) in mediating neuroprotection, the neuroprotective profiles of selective ER agonists for ERalpha and ERbeta, propylpyrazole triol (PPT) and 2,3-bis(4-hydroxyphenyl) proprionitrile (DPN), respectively, were compared to that of 17beta-estradiol and 17alpha-estradiol i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.03.032
更新日期：2005-05-31 00:00:00
abstract：:The effects of induced L9 activity on gill motor neuron L7's ability to elicit a gill withdrawal response were studied. It was found that L9 was a modulator of L7's effectiveness to elicit gill movements. Following L9 activity, L7's ability to elicit a gill withdrawal response was significantly potentiated by up to 24...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90350-0
更新日期：1979-03-16 00:00:00
abstract：:It has recently been shown that the immunosuppressant cyclosporin A (CsA) dramatically ameliorates the selective neuronal necrosis which results from 10 min of forebrain ischemia in rats. Since CsA is a virtually specific blocker of the mitochondrial permeability transition (MPT) pore which is assembled under adverse ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00005-x
更新日期：1997-04-04 00:00:00
abstract：OBJECTIVE:To investigate the effect of salvinorin A (SA) on brain injury and neurologic function post-brain ischemia/reperfusion (I/R) using a rat forebrain ischemia model and further explore the effect of kappa opioid receptor (KOR) inhibition by SA on aquaporin-4 (AQP4) expression in the hippocampus, cortex and stria...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.02.024
更新日期：2016-04-15 00:00:00
abstract：:Retinae taken from embryonic mice were transplanted to the brain of newborn rats. One eye was removed from the recipients at this time to promote innervation of host visual centers. After 4-6 weeks the optic nerve of the remaining eye was transected to eliminate all visual afferents of host origin and 4-6 days later t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91338-h
更新日期：1990-11-19 00:00:00
abstract：:The present study compared Fos expression in identified hypothalamic magnocellular neurons in lactating and non-lactating female rats submitted to acute haemorrhage or 24 h of water deprivation, stimuli that induce the release of both oxytocin and vasopressin. Quantitative analysis of preparations doubly immunostained...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90809-5
更新日期：1994-10-31 00:00:00
abstract：:When female golden hamsters are maintained on a photoperiod of less than 12.5 h of light per day, they go into a pineal gland-induced anestrus in 6-10 weeks. This acyclicity is similar in certain respects to the testicular regression which occurs in male golden hamsters maintained on a short photoperiod. A recent stud...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91428-9
更新日期：1986-08-13 00:00:00
