Cytological investigations of the interspecific hybrids of Nicotiana tabacum L. x N. glauca Grah.

Abstract:

:Interspecific amphihaploid and amphidiploid hybrids between Nicotiana glauca Grah. (2n = 24) and N. tabacum L. (2n = 48) cultivars BY 103 and K 326 were analysed. F1 amphihaploids (2n = 36) were viable and completely self- and cross-sterile, and mostly univalents were present during meiosis (with pairing range from 0 to 5). In some meiocytes, meiotic irregularities were observed, such as sporadic chromatin bridges and formation of restitution nuclei. The resultant F1 hybrids were easily converted to amphidiploids (2n = 72) via colchicine treatment of seedlings. The number of univalents and the frequency of PMCs containing unpaired chromosomes indicated that amphidiploids N. tabacum cv. BY 103 or K 326 x N. glauca represented quite a high pairing category. However, they were male sterile because pollen mother cells were arrested at the tetrad stage. The termination of development of PMCs, and consequently male sterility, are very rare in this kind of tobacco hybrids.

journal_name

J Appl Genet

authors

Trojak-Goluch A,Berbeć A

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

45-54

issue

1

eissn

1234-1983

issn

2190-3883

pii

131

journal_volume

44

pub_type

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