MMP-9 and EBA immunoreactivity after papaverine mediated opening of the blood-brain barrier.

Abstract:

:Papaverine is a non-specific vasodilatory drug, which was found to unexpectedly cause opening of the blood-brain barrier (BBB). We studied the expression of two endothelial cell related markers, endothelial barrier antigen (EBA) as a marker of BBB intactness, and matrix metalloproteinase-9 (MMP-9), an extracellular matrix molecule, the activation of which results in BBB opening. Immunoreactivity was quantified after intra-carotid injection of papaverine in rats. BBB opening was identified at 1 h, but had been reversed by 24 h. Reduction in EBA-immunoreactivity occurred at 3 h, whereas MMP-9 was not observed until 24 h. The activation of MMP-9 thus occurred much later than BBB opening, which suggests a different role for MMP-9 when selective and reversible opening of the BBB is produced by pharmacological manipulation.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Bhattacharjee AK,Kondoh T,Ikeda M,Kohmura E

doi

10.1097/00001756-200212030-00011

subject

Has Abstract

pub_date

2002-12-03 00:00:00

pages

2217-21

issue

17

eissn

0959-4965

issn

1473-558X

journal_volume

13

pub_type

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