Abstract：
:Second- and higher-order degradation reactions sometimes cannot be approximated with linear or exponential relationships and need to be appropriately modeled. Events above the COULTER HmX Analyzer white blood cell (WBC) counting threshold were recorded for the HmX PAK reagent system stored at five elevated temperatures. An accelerated stability model for a second-degree polynomial degradation pattern was used. The shelf-life of the reagent, along with 95% lower bound confidence intervals, is predicted using the same pattern of degradation as well as the Arrhenius approximation. Experiments indicated that the degradation of the HmX PAK reagent occurred in two phases, the lag phase and the degradation phase, in all tested temperatures. The phase durations are temperature-dependent, and the Arrhenius approximation is appropriate (P=0.639). The degradation of the reagent during the lag phase was experimentally undetectable. Changes of the reagent were nonsignificant for a predicted period of 164 days at 25 degrees C. The rate of degradation increased significantly later on during the degradation phase. The lower bound of the 95% confidence interval of this prediction indicated that it would take at least 326 days before the HmX PAK reagent would have any performance issue related to aging at storage temperature.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Magari RT,Murphy KP,Fernandez Tdoi
10.1002/jcla.10043subject
Has Abstractpub_date
2002-01-01 00:00:00pages
221-6issue
5eissn
0887-8013issn
1098-2825journal_volume
16pub_type
杂志文章abstract：BACKGROUND:The prevalence of diabetes is increasing in China. Glucose control is very important in diabetic patients. The aim of this study was to compare the accuracy of five glucose meters used in Chinese hospitals with a reference method, in the absence and presence of various factors that may interfere with the met...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21611
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:This study aimed at exploring the correlation of microRNA (miR)-497/fibroblast growth factor-23 (FGF-23) axis with major adverse cardiac and cerebral event (MACCE) occurrence in end-stage renal disease (ESRD) patients who underwent continuous ambulatory peritoneal dialysis (CAPD). METHODS:Totally, 360 ESRD p...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23220
更新日期：2020-06-01 00:00:00
abstract：:Congenital hypoplastic anemias are a rare and heterogeneous group of disorders. This paper reviews new molecular diagnostic tests in two distinct forms of congenital anemias, anemia due to transplacental infection with B19 parvovirus and Fanconi anemia. In both instances, molecular assays making use of amplification o...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis. METHODS:Brucella Coombs gel test (BCGT), Brucella ELISA (IgG + I...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21934
更新日期：2016-09-01 00:00:00
abstract：:In order to analyze the epitope structure of carcinoembryonic antigen (CEA) and the idiotype network system, seven anti-idiotypic monoclonal antibodies (anti-Id MoAbs) were generated from a BALB/c mouse immunized with anti-CEA MoAb P1-356, which recognized a synthetic peptide P1 of CEA, domain I. These MoAbs were divi...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.10058
更新日期：2002-01-01 00:00:00
abstract：:An enzyme-linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum-absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum-absorbed te...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860040210
更新日期：1990-01-01 00:00:00
abstract：:Previously, an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for p24 antigen of HIV-1 was developed. The immune complex comprising 2,4-dinitrophenyl-biotinyl-bovine serum albumin-rabbit anti-p24 Fab' conjugate, p24 antigen, and rabbit anti-p24 Fab' -beta-D-galactosidase conjugate was t...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2825(1996)10:5<302::AID-JCLA11>
更新日期：1996-01-01 00:00:00
abstract：:Multidrug resistance (MDR) is a multifactorial phenomenon and the role of these proteins in generating the MDR phenotype is controversial. With this in mind, this review compiled the current data on the role of ABCB1, ABCC1, and LRP proteins in the prognosis of hematologic neoplasms and their influence on the choice o...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章,评审
doi：10.1002/jcla.21563
更新日期：2013-01-01 00:00:00
abstract：:Elevated serum chromogranin A (CgA) levels have been detected in patients with prostate cancer who have developed resistance to hormonal therapy. We would like to reexamine these cases by using serial specimens to determine whether such elevated levels are also detectable in prostate cancer patients not undergoing hor...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Delta neutrophil index (DNI) is a new inflammatory marker and the present study aimed to evaluate the predictive value of the DNI for the presence of a perforation in elderly with acute appendicitis. METHODS:This retrospective observational study was conducted on 108 consecutive elderly patients (≥65 years ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22177
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:This study aimed to investigate the relationship between HbA1c and RBC aggregation in type 2 diabetes mellitus (T2DM) patients by analysis of data from routine clinical tests and from in vitro experiments. METHODS:A total of 2,111 inpatients with type 2 diabetes were selected and among them, 364 patients (G...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21784
更新日期：2015-09-01 00:00:00
abstract：BACKGROUND:The aim of this study is to evaluate the clinical significance of cystatin C(CysC) in the newborns who show normal serum creatinine (Cr) and who are in an intensive care unit. METHODS:From July 2009 to May 2010, a total of 106 patients (53 male and 53 female newborns) in a neonatal intensive care unit at Ky...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21516
更新日期：2012-07-01 00:00:00
abstract：BACKGROUND:MYBPC3 mutations have been described in dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM). A mutation, c.3373G>A, has been reported to cause autosomal recessive form of HCM. Here, we report that this mutation can cause autosomal dominant form of DCM. METHODS:Next-generation sequencing using...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22419
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND:Lupus nephritis is a serious manifestation of systemic lupus erythematosus (SLE). The objective of this study was to identify the sensitivity, specificity, and cut-off values of IP-10 in the serum and urine of patients with lupus nephritis compared to renal biopsy, albumin/creatinine ratio, and serum anti-ds...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21993
更新日期：2016-11-01 00:00:00
abstract：:United States Food and Drug Administration (FDA) in 2010 approved the use of immunosuppressant drug everolimus, which requires therapeutic drug monitoring in whole blood. Taking advantage of structural similarity between sirolimus and everolimus we attempted to rapidly estimate everolimus concentration from apparent s...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20459
更新日期：2011-01-01 00:00:00
abstract：:Urinary proteins from six patients with esophageal cancer and two with stomach cancer were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analyses were performed on days-1 to 3, 5, 7, 10, 14, and 21 (or 22) after surgery. The protein patterns were scanned by densitometry and divided ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20087
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:Recently, several studies have investigated the relationship between Pre-miR-27a rs895819 polymorphism and risk of various cancers. However, the relationship between rs895819 and diffuse large B-cell lymphoma (DLBCL) has not been well known. METHODS:In this study, we conducted a case-control study to explor...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23088
更新日期：2020-03-01 00:00:00
abstract：BACKGROUND:We evaluated the effects of pneumatic tube system (PTS) transport rates and distances on routine hematology and coagulation analysis. PTS effects on centrifuged blood samples were also examined. METHOD:The study was completed at Dicle University Hospital, which has the longest pneumatic tube system in Turke...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21484
更新日期：2012-02-01 00:00:00
abstract：BACKGROUND:Bone marrow (BM) study plays an important role as initial investigation specimen of lymphoma as well as staging lymphoma. This study aimed to investigate the utility of BM studies for classification of lymphoma and evaluate features of BM involvement by lymphoma over a period of 11 years. METHODS:A total of...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22841
更新日期：2019-05-01 00:00:00
abstract：AIM:To investigate the presence of antipituitary antibodies (APA) in the serum of patients undergoing gastroenteropathies (GEP) other than celiac disease (CD). METHODS:APA were analyzed in GEP patients (n = 103), CD patients (n = 94), idiopathic growth hormone (GH) deficiency patients (n = 21), and 98 age- and sex-mat...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21644
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Alport syndrome (ATS) is a hereditary nephritis with hereditary and clinical heterogeneity; the early clinical symptoms are atypical, which can easily lead to misdiagnosis. The proband, a 6-year-old girl, was found to have microscopic hematuria, proteinuria, and visual impairment at about 5 years old; the re...
journal_title：Journal of clinical laboratory analysis
pub_type：
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更新日期：2020-09-06 00:00:00
abstract：:Mycobacterium avium is an important intracellular pathogen, particularly in AIDS patients. It also shows the second frequency among nontuberculous mycobacteria infections in Korea. Point mutations of domain V region of the 23S rRNA gene has been known to confer clarithromycin resistance to M. avium. In order to isolat...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20403
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:Diverse and circumstantial evidence suggests that schizophrenia is a neurodevelopmental disorder. Genes contributing to neurodevelopment may be potential candidates for schizophrenia. The human SOX11 gene is a member of the developmentally essential SOX (Sry-related HMG box) transcription factor gene family ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23306
更新日期：2020-08-01 00:00:00
abstract：:Recently, Siemens Diagnostics released a new calcium assay (CA_2) based on complex formation of calcium with Arsenazo III dye for use on the three automated, random access ADVIA Chemistry analyzers (1650, 2400, and 1200). We evaluated this method for analytical performance as well as potential interference from gadoli...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20353
更新日期：2009-01-01 00:00:00
abstract：:We aimed to evaluate whether a PEG-IAT method, modified for gel technique, could replace the two-stage enzyme method for routine identification of red blood cell (RBC) antibodies.A total of 161 selected patient samples were analyzed with the two-stage enzyme tube technique and the PEG-IAT in gel techniques. In 65 samp...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20017
更新日期：2004-01-01 00:00:00
abstract：:Polyfunctional hydrophilic microspheres of 125-nm diameter can be produced by copolymerization of acrylic monomers. Purified c-reactive protein (CRP) was covalently bound to these new micropheres, and the conjugate obtained was used as reagent in a microparticle-enhanced nephelometric immunoassay for human CRP. This a...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860060106
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:Multiple organ dysfunction syndromes (MODS) is reported as a leading cause of mortality in intensive care units. Recently, continuous blood purification (CBP) has been mostly applied for MODS treatment. Thus, the purpose of this study was to investigate the effects of CBP on plasma phospholipid level in pati...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22411
更新日期：2018-07-01 00:00:00
abstract：:Two methods for the extraction of RNA of vesicular stomatitis virus (VSV) Indiana1 and New Jersey and their simultaneous amplification by one-step polymerase chain reaction using reverse transcriptase were evaluated. A guanidine-thiocyanate-based RNA extraction (Qiagen RNeasy Mini Kit, Qiagen, Valencia, CA ) followed ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20439
更新日期：2011-01-01 00:00:00
abstract：:A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominantly GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860040503
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND:Previous studies suggested that adiponectin (APN) could ameliorate ischemia/reperfusion injury and endothelial dysfunction in patients with acute myocardial infarction. However, the relationship between serum APN level and coronary flow after primary percutaneous coronary intervention (PPCI) in patients with...
journal_title：Journal of clinical laboratory analysis
pub_type： 临床试验,杂志文章
doi：10.1002/jcla.22864
更新日期：2019-06-01 00:00:00
