Uptake of 45Ca by mitochondria of Trigonella foenum-graecum as influenced by selenium and mimosine--detailed kinetic analyses.

Abstract:

:Mitochondria from Trigonella foenum-graecum seedlings grown independently in the presence of either selenium (0.75 ppm) or mimosine (0.1 mM) exhibited respiration-stimulated energy-dependent uptake of Ca2+. Uptake studies were carried out independently at a series of Ca2+ concentrations at two different levels: (1) 1-20 MM and (2) 25-1,500 microM. Levels of uptake were 50-100% higher in the mitochondria of seedlings of both the Se and mimosine groups. Detailed kinetic analyses revealed negative cooperative effects operative during uptake of Ca2+ at 25-1,500 microM given in the medium. Hill coefficients for Ca2+ uptake by the mitochondria of different groups remained unchanged (nH, 0.75). Biphasic Scatchard plots were concave upward, suggestive of two classes of binding sites. High-affinity binding sites were estimated to be 16 nmol/mg protein with dissociation constant (KCa) of 2.5 x 10(9) L/mol. In contrast, graphical analyses of the uptake of Ca2+ in the range 1-20 microM in the medium revealed cooperative effects of positive nature. The present study demonstrates mixed cooperative effects during Ca2+ uptake by mitochondria from seedlings of T. foenum-graecum.

journal_name

Biol Trace Elem Res

authors

Sreekala M,Lalitha K

doi

10.1385/BTER:82:1-3:217

subject

Has Abstract

pub_date

2001-07-01 00:00:00

pages

217-29

issue

1-3

eissn

0163-4984

issn

1559-0720

pii

BTER:82:1-3:217

journal_volume

82

pub_type

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