Abstract：
:Culture of preimplantation embryos is complex and requires strictly defined culture media to sustain their viability and quality. In the current study, an effort was made to evaluate comprehensively the quality of mice embryos, grown in media enriched with IGF I, IGF II, EGF and TNFalpha. For that purpose, critically chosen and thoroughly described, complex morphological methods based on contrast-phase, fluorescent and confocal microscopy were used. The study evaluated blastulation and hatching rates, total blastocyst cells, inner cell mass cell numbers (differential staining) as well as identified embryo cells with positive reactions for necrosis or apoptosis (TUNEL). The critical evaluation of the effects of the studied cytokines allowed for simultaneous, meticulous assessment of the applied study methods. Significantly more blastocysts were found in culture media enriched with IGF-I, IGF II and EGF. Significantly more hatched blastocysts were found in media with IGF-I and IGF II. Additionally, IGF I and II increased inner cell mass and total blastocyst cell numbers. Very few cells with necrosis and apoptosis were found in the culture media enriched with IGF I, IGF II and EGF. TNFalpha produced negative effects. The observed effects were dose-dependent.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Kurzawa R,Głabowski W,Wenda-Rózewicka Lsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
245-51issue
3eissn
0239-8508issn
1897-5631journal_volume
39pub_type
杂志文章abstract：:Mucins have been shown to be aberrantly overexpressed in various diseases including cystic fibrosis, asthma, and cancer. Recent studies have uncovered the roles of these mucins in the pathogenesis of cancer. The presence of MUC-1 has also been detected on the cell surface of multiple myeloma (MM) cells in peripheral b...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0089
更新日期：2011-01-01 00:00:00
abstract：:The ability to preselect the sex of human offspring would have a significant impact on the preventing of sex chromosome-linked diseases and on human reproduction. Numerous methods have been reviewed including: Sephadex gel filtration, centrifugation in density gradients, separation in albumin gradient, laminar flow, c...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:Lipotubuloids, structures containing lipid bodies and microtubules, are described in ovary epidermal cells of Ornithogalum umbellatum. Microtubules of lipotubuloids can be fixed in electron microscope fixative containing only buffered OsO(4) or in glutaraldehyde with OsO(4) post-fixation, or in a mixture of OsO(4) and...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0040
更新日期：2011-01-01 00:00:00
abstract：:The level of transglutaminase activity has been investigated by the fluorescence method in peritoneal macrophages of control and transplantable melanomas bearing hamsters. If the transglutaminase activity is concerned the macrophages in no one of the groups examined were a homogenous population. Differences in macroph...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:The induction of exercise-induced apoptosis in not actively involved in exercise organs, such as kidney could be a result of oxidative stress. Metallothionein (MT) exerts a protective effect in the cell against oxidative stress and apoptosis. We have previously demonstrated an increased incidence of apoptosis in dista...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:On the basis of radioactivity of interphase cells in the antheridial filaments of Chara vulgaris after a 20 min. incubation with 3H-uridine and after a 40 min. postincubation the activity of synthesis and transport of newly synthesized RNA has been studied in different-sized nucleoli. Investigations of their ultrastru...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Embryo implantation consists of a series of events promoting the invasion of the endometrium and then the uterine arterial system by the extra-embryonic trophoblast. In order for this semi-heterologous implantation to succeed, the endometrium has to first undergo a number of structural and biochemical changes (decidua...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0058-3
更新日期：2009-01-01 00:00:00
abstract：:In this study, the histological, immunohistochemical, morphometric, and biochemical changes to pancreatic beta-cells in STZ-induced diabetes were evaluated in rats treated with different doses of caffeine. Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control group, the diabetic un...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.2014.0005
更新日期：2014-01-01 00:00:00
abstract：:The supramolecular dye Congo red was used to check whether monocyte activation may be mediated by a torsion-dependent mechanism preventing transduction of weak random signals in cell contacts in a way corresponding to the discrimination mechanism found in complement fixation by immune complexes. Tight cell-cell contac...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:The goal of our study was to assess suitability of FNA biopsy material as a source of samples (cell suspension) for DNA ploidy assessment in neoplastic tumors using flow cytometry. DNA ploidy is an established prognostic factor in many types of cancers. Aneuploid breast tumors are characterized by increased aggressive...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:A child was operated 3 times because of a recurrent growth hormone- and prolactin-producing pituitary adenoma. Between the operations she was treated for five years with bromocriptine. The characteristics of the tumour cell population collected after the last operation was now examined by electron microscopy, immunocy...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Progress in recognition of structure and function in a variety of cells has always been linked to development of new research techniques. Beyond doubt, immunocytochemical techniques belong to such new methods. However, the techniques had not been widely applied to electron microscopy until colloidal gold was introduce...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：:The fossil dinosaur bone material, 80 million years old was studied. Samples for analysis were prepared with specially elaborated methods. Images obtained in the light, transmission electron, and scanning electron microscopes revealed the spatial distribution of the osteocytes lying near and far from the vascular cana...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Recently identified biological risk factors in B-cell chronic lymphocytic leukemia (B-CLL) include ZAP-70 and CD38 expression. The present study was conducted to clarify whether a combined analysis could improve predictive impact of these two parameters. We examined the expression of ZAP-70 and CD38 by flow cytometry ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0022-7
更新日期：2008-01-01 00:00:00
abstract：INTRODUCTION:NKT-like cells are "non-classical", "CD1d-independent" NKT cells which represent highly differentiated, conventional T lymphocytes coexpressing several NK (natural killer) associated receptors. They are effector lymphocytes of both innate and adaptive immunity and simultaneously regulatory cells of the ada...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2018.0025
更新日期：2018-01-01 00:00:00
abstract：:After dmPGE2 treatment, both alone or together with streptozotocin, the lower, statistically significant total PL contents (in mumoles P per mg of protein) in rat liver Golgi-rich membrane fractions were found. In these groups of investigated rats, the percentage of PE+PA were statistically significantly lower than in...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:The present work deals with the histochemical, histoenzymological and micro-analytical study of the stomach of Littorina Littorea (L.). The results suggest the existence of intracellular digestion within the stomach cells. Thus, the occurrence of lysosomes in the mid-cytoplasm of stomach cells has been revealed by dem...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:During alpaca testis development and spermatogenesis, nerve growth factor (NGF) may play an important role. The main aim of this study was to determine the expression and localization of NGF in the alpaca testis, and to discuss the important role of NGF in alpaca reproductive characteristics. Immunohistochemical stain...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0009
更新日期：2011-01-01 00:00:00
abstract：:Ventral tegmental area (VTA) is a heterogeneous group of dopaminergic cells which contains interfascicular (IF), parabrachial (PBP) and rostral linear (RLi) nuclei. Neurons of this area are involved in the regulation of motor and motivational aspects of behavior and reveal high neuronal plasticity. Among many various ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:CD59 is one of the key molecules involved in cell protection against autologus complement. The fact that complement regulatory proteins are able to prevent hyperacute rejection of organs in pig to primate model, raises the question of possible complement regulatory protein (CRP) involvement in the maturation of immuno...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:The intra-S-phase checkpoint response to hydroxyurea (HU)-mediated arrest of DNA replication was analysed in root meristems of two legumes, Pisum sativum and Vicia faba. The obtained results suggest that a molecular signal which invokes mechanisms allowing the cells to override the S-M dependency control system may be...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：INTRODUCTION:The function and localization of actin in the nucleus have not yet been fully described. However, actin seems to be a key protein in nuclear processes interacting with chromatin and matrix proteins. The aim of the study was to evaluate the effect of controlled expression of nuclear pool of F-actin and spec...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2015.0008
更新日期：2015-01-01 00:00:00
abstract：:Immunohistochemical localization of androgen receptor (AR) was investigated in the rat oocytes during their development in the primary, secondary and antral follicles. The group of experimental Wistar rats included prepubertal female rats, killed at 30 days of age, and mature female rats killed at estrus or metestrus....
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:In male sex organs of Chara vulgaris L., the gibberellic acid (GA3), was identified by capillary zone electrophoresis. The antheridia at cell division stage of antheridial filaments leading to formation of spermatids contain 0.09 microg GA3 per antheridium, i.e. 5.3 times more than antheridia at differentiation stage ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：INTRODUCTION:Aging causes morphological and functional changes in the thyroid gland. Free radicals play a key role in the pathology of normal aging. Vimentin and cytokeratin are cytoskeletal intermediate filaments that are often used as indirect indices of tissue injury. The aim of the study was to clarify the age-rela...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2018.0015
更新日期：2018-01-01 00:00:00
abstract：:Combined (3H) estradiol autoradiography and oxytocin immunocytochemistry were used in order to study co-localization of cytoplasmic oxytocin immunoreactivity and nuclear uptake of (3H) estradiol in the forebrain of adult ovariectomized mice. Labelling with (3H) estradiol was found in subpopulations of neurons that con...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Tuberculosis (TB) still remains the leading cause of mortality due to bacterial pathogen. The only currently available vaccine against TB, Bacille Calmette-Guérin (BCG) is at best credited with a 50% overall protective efficacy. Skin testing with purified protein derivative (PPD) from Mycobacterium tuberculosis is the...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0044-1
更新日期：2008-01-01 00:00:00
abstract：:Factors controlling complement activation appear to exert a protective effect on pregnancy. This is particularly important in women with thrombophilia. The aim of this study was to determine the transcript and protein levels of complement decay-accelerating factor (DAF) and membrane cofactor protein (MCP) in the place...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/19758
更新日期：2012-10-08 00:00:00
abstract：:Stem cells for skeletal muscle originate from dermomyotome of the embryo. The early marker of these cells is expression of both transcription factors Pax3 and Pax7 (Pax3+/Pax7+ cells). The skeletal muscles in the adult organism have a remarkable ability to regenerate. Skeletal muscle damage induces degenerative phase,...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：2006-01-01 00:00:00
abstract：:Therapeutic outcomes of definitively treated non-small-cell lung cancer (NSCLC) are unacceptably poor. It has been proposed that the manipulation of dendritic cells (DCs) as a "natural" vaccine adjuvant may prove to be a particularly effective way to stimulate antitumor immunity. Presently, there is no standardized me...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0078-4
更新日期：2008-01-01 00:00:00
