Abstract：
:Factors controlling complement activation appear to exert a protective effect on pregnancy. This is particularly important in women with thrombophilia. The aim of this study was to determine the transcript and protein levels of complement decay-accelerating factor (DAF) and membrane cofactor protein (MCP) in the placentas of women with acquired and inherited thrombophilia. Also, we assessed immunohistochemistry staining of inhibitors of the complement cascade, DAF and MCP proteins, in the placentas of thrombophilic women.Placentas were collected from eight women with inherited thrombophilia and ten with acquired thrombophilia.The levels of DAF and MCP transcripts were evaluated by qPCR, the protein level was evaluated by Western blot. We observed a higher transcript (p < 0.05) and protein (p < 0.001) levels of DAF and MCP in the placentas of thrombophilic women than in the control group. DAF and MCP were localized on villous syncytiotrophoblast membranes, but the assessment of staining in all groups did not differ. The observed higher expression level of proteins that control activation of complement control proteins is only seemingly contradictory to the changes observed for example in the antiphospholipid syndrome. However, given the hitherto known biochemical changes associated with thrombophilia, a mechanism in which increased expression of DAF and MCP in the placentas is an effect of proinflammatory cytokines, which accompanies thrombophilia, is probable.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Wirstlein PK,Jasiński P,Rajewski M,Goździewicz T,Skrzypczak Jdoi
10.5603/19758subject
Has Abstractpub_date
2012-10-08 00:00:00pages
460-7issue
3eissn
0239-8508issn
1897-5631pii
VM/OJS/J/19758journal_volume
50pub_type
杂志文章abstract：:Using immunohistochemistry and Western blot analysis we attempted to identify the estrogen receptors in ovine luteal cells at different stages of the estrous cycle. Monoclonal antibody against estrogen receptors was used for immunolocalization of estrogen receptor-alpha in corpora lutea sections. Generally, the most i...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:In cervical cancer, glycosylation has been suggested as being involved in both its carcinogenesis and invasive capacity. In this work, we analyzed mucin type O-glycosylation in biopsies of invasive cervical cancer in FIGO stage II B through histochemistry using lectins specific for O-glycosidically linked glycans. Our...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/19748
更新日期：2012-10-08 00:00:00
abstract：:Epithelial ovarian cancer represents one of the most deadly gynaecological neoplasms in developed countries and is a highly heterogeneous disease. Epidemiological studies show that anti-inflammatory drugs reduce the incidence and mortality of several types of cancer, indicating the potential role of pro-inflammatory f...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.2014.0015
更新日期：2014-01-01 00:00:00
abstract：:The aim of our study was to investigate the dynamics of the alterations of soluble human leukocyte antigen-G (sHLA-G) concentrations in sera of healthy non-pregnant women, as well as healthy pregnant women and patients with pre-eclampsia. Thirty five patients with pre-eclampsia, 52 healthy pregnant women, and 24 healt...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2012.0023
更新日期：2012-07-05 00:00:00
abstract：:Morphology and some molecular aspects of hyperplastic (bronchial basal cell hyperplasia and alveolar cell hyperplasia), metaplastic (squamous metaplasia), preneoplastic and early neoplastic (dysplasia in squamous metaplasia, cancer in situ and atypical alveolar cell hyperplasia) changes were studied in 180 lungs resec...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The NADPH-diaphorase (NADPH-d) reaction is frequently used to visualize the diaphorase activity of nitric oxide synthase (NOS). However, this tetrazolium salt procedure can be of limited specificity at sites where non-specific alkaline phosphatase (alP) and NADHd activity co-exist. This is shown in the present paper u...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Light microscopic histochemical studies to visualize semicarbazide-sensitive and H2O2-generating amine oxidase (SSAOX, benzylamine oxidase, BAOX; EC 1.4.3.6?) are usually performed with the coupled peroxidatic oxidation technique of Ryder et al. [25]. For methodological reasons this procedure has its limitations and w...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The aim of this study was to examine the prevalence and clinicopathological significance of KRAS point mutation in endometrial hyperplasia and carcinoma. We analysed KRAS in 11 cases of complex atypical hyperplasia and in 49 endometrial carcinomas using polymerase chain reaction associated with restriction fragment le...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0014-2
更新日期：2009-01-01 00:00:00
abstract：:Immunohistochemical localization of androgen receptor (AR) was investigated in the rat oocytes during their development in the primary, secondary and antral follicles. The group of experimental Wistar rats included prepubertal female rats, killed at 30 days of age, and mature female rats killed at estrus or metestrus....
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Therapeutic outcomes of definitively treated non-small-cell lung cancer (NSCLC) are unacceptably poor. It has been proposed that the manipulation of dendritic cells (DCs) as a "natural" vaccine adjuvant may prove to be a particularly effective way to stimulate antitumor immunity. Presently, there is no standardized me...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0078-4
更新日期：2008-01-01 00:00:00
abstract：:The increased ambient temperature affects the function of hypothalamic-pituitary-adrenal (HPA) axis. Since the correlation among vasopressin (VP), adrenocorticotropic hormone (ACTH) and corticosterone (CORT) responses to various stressors have been long recognized, the aim of this study was to reveal the aforementione...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-010-0071-6
更新日期：2010-12-01 00:00:00
abstract：:Delayed type hypersensitivity reaction (DTH) consists of a sequential cascade of steps depending on different types of T cells, as well as mast cells, endothelial cells and macrophages. Recently it has been shown that CD4+ TH1 lymphocytes ("inflammatory type") play a central role in DTH reaction. Activated TH1 cells p...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：1990-01-01 00:00:00
abstract：:The biology of breast cancer is closely releted to sex steroid hormones. Estrogen receptor beta is overexpressed in around 70% breast cancer cases, referrd to as "ER positive". Estrogens bind to estrogen receptor and stimulate the transcription of genes involved in control of cell proliferation. Moreover, estrogens ma...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0047-6
更新日期：2009-01-01 00:00:00
abstract：:In telotrophic ovary of Creophilus maxillosus, the differentiation of the oocyte and nurse cells takes place within the linear clusters of sister oogonial cells. The amplification of rDNA occurs in the nuclei of pro-oocytes which are the most posterior cells of the clusters. During the consecutive oogonial divisions e...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：INTRODUCTION:The function and localization of actin in the nucleus have not yet been fully described. However, actin seems to be a key protein in nuclear processes interacting with chromatin and matrix proteins. The aim of the study was to evaluate the effect of controlled expression of nuclear pool of F-actin and spec...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2015.0008
更新日期：2015-01-01 00:00:00
abstract：:The study aimed at examination of tissue expression of polysaccharides and secretory mucin 5AC (MUC5AC) in young patients (up to 25 years of age) with a symptomatic gallstones. For comparison, patients most frequently subjected to cholecystectomy were studied, i.e. patients of approximately 50 years of age with the sa...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-010-0053-8
更新日期：2010-12-01 00:00:00
abstract：:Lipotubuloids, structures containing lipid bodies and microtubules, are described in ovary epidermal cells of Ornithogalum umbellatum. Microtubules of lipotubuloids can be fixed in electron microscope fixative containing only buffered OsO(4) or in glutaraldehyde with OsO(4) post-fixation, or in a mixture of OsO(4) and...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0040
更新日期：2011-01-01 00:00:00
abstract：:Two hypotheses explain the role of adult progenitor cells in myocardial regeneration. Stem cell plasticity which involves mobilization of stem cells from the bone marrow and other niches, homing to the area of tissue injury and transdifferentiation into functional cardiomyocytes. Alternative hypothesis is based on the...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：2005-01-01 00:00:00
abstract：:Cancer procoagulant activity in the blood serum of patients with oesophagal, gastric and colorectal cancer was evaluated before and after the tumour removal. Cancer procoagulant activity was significantly higher before the operation in comparison to the control group and was reduced after a total operative procedure, ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The influence of 48-h treatment with epoxomicin, an inhibitor of proteolytic activity of proteasomes, at the concentration 10 microM, on spermiogenesis in algae Chara vulgaris was examined. In the presence of the inhibitor, the frequency of early spermiogenesis phases significantly increased, the number of spermatids ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:CD59 is one of the key molecules involved in cell protection against autologus complement. The fact that complement regulatory proteins are able to prevent hyperacute rejection of organs in pig to primate model, raises the question of possible complement regulatory protein (CRP) involvement in the maturation of immuno...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:The endemic Rhodanian percid Zingel asper (Linnaeus, 1758), is usually found throughout the Rhône basin, but this fish is now in sharp decline. Understanding its reproductive physiology is important so as to be able to artificially control its reproduction with a view to re-introducing it. This study was carried out o...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0069
更新日期：2011-01-01 00:00:00
abstract：:The effect of estradiol and progesterone on the noradrenaline (NA) content in nerve fibres of the oviduct, uterus and vagina was investigated in 37 ovariectomized sows, divided into 4 groups. A recovery period was 4 weeks. Starting from the 9th day before killing, the animals were injected 10 times (1 injection/day; t...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The present work deals with the histochemical, histoenzymological and micro-analytical study of the stomach of Littorina Littorea (L.). The results suggest the existence of intracellular digestion within the stomach cells. Thus, the occurrence of lysosomes in the mid-cytoplasm of stomach cells has been revealed by dem...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The advent of DNA and RNA labelling techniques which allow non-autoradiographic detection of specific nucleic acid sequences has opened new possibilities for rapid diagnosis at the nucleic acid level. There are already practical applications. However, in order to fully compete with radioactive procedures an increase i...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：INTRODUCTION:Chronic rhinosinusitis (CRS) affects 14% of the world population. The high motility group box 1 (HMGB1) protein triggers inflammation, cell proliferation and cell survival through its receptor for advanced glycation end products (RAGE) upon release from stressed or necrotic cells. The aim of the study was ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2015.0007
更新日期：2015-01-01 00:00:00
abstract：:Human chromosome carry different fragile sites, but the most interesting one is the fragile X chromosome, especially in relation to mental retardation. In this report we present micronucleus formation in 5 individuals with the fragile X syndrome and 11 control subjects whose lymphocytes were cultured under 1) hydroxyu...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Antibovine leukocyte monoclonal antibodies were tested in order to use them for scanning electron microscopy in backscattered electron imaging mode. The tests revealed that numerous antibovine leukocyte monoclonal antibodies still recognize lightly glutaraldehyde prefixed antigens and can be used to identify various b...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Parathyroid hormone-related peptide (PTHrP) is the cause of humoral hipercalcaemia of malignancy syndrome (HHM). It is known that the peptide as well as its receptors are widely distributed in many normal organs and tissues, where it influences an array of diverse functions which are realized through paracrine or auto...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:The effects of captopril (the inhibitor of the angiotensin-converting enzyme) and of angiotensins II and IV (3-8 fragment of angiotensin II) on cell proliferation of the prostatic epithelium was investigated in the rat. The incorporation of bromodeoxyuridine into cell nuclei was used as an index of cell proliferation....
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
