Abstract：
INTRODUCTION:The function and localization of actin in the nucleus have not yet been fully described. However, actin seems to be a key protein in nuclear processes interacting with chromatin and matrix proteins. The aim of the study was to evaluate the effect of controlled expression of nuclear pool of F-actin and special AT-rich sequence-binding protein 1 (SATB1) on the in vitro induction of active cell death by geldanamycin (GA). MATERIAL AND METHODS:The expression of SATB1 was regulated by the transfection of non-aggressive breast cancer MCF-7 cells with siRNA against SATB1 or expression plasmid with cloned cDNA of SATB1. The altered expression of cofilin-1 in these cells was used to regulate the nuclear expression and localization of F-actin. The effect of GA was analyzed in the context of cell death induction and cell cycle alterations. RESULTS:Our studies revealed that the targeted regulation of SATB1 and cofilin-1 expression changed the apoptotic response of the MCF-7 cells to GA. The overexpression of these proteins potentiated GA-induced arrest of the cells in the G1 phase of cell cycle and increased the population of the hypodiploid cells. CONCLUSION:The alterations in the nuclear expression of SATB1 and F-actin in MCF-7 cells may affect their active cell death in response to GA.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Grzanka D,Izdebska M,Klimaszewska-Wisniewska A,Gagat Mdoi
10.5603/FHC.a2015.0008subject
Has Abstractpub_date
2015-01-01 00:00:00pages
79-87issue
1eissn
0239-8508issn
1897-5631pii
VM/OJS/J/41484journal_volume
53pub_type
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journal_title：Folia histochemica et cytobiologica
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：10.2478/v10042-008-0003-x
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journal_title：Folia histochemica et cytobiologica
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更新日期：2002-01-01 00:00:00
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