Abstract:
:Fertilization involves a series of cellular interactions culminating in the fusion of gamete membranes, creating a zygote and then an embryo. During the process of human fertilization in vivo or in conventional in vitro fertilization (IVF), sperm must be capable of undergoing the acrosome reaction, binding to the zona pellucida (ZP), and penetrating the ZP to fuse with the oolema. The key role in this process is played by fertilin beta. Protamines and histones are the proteins that bind to sperm chromatin and contribute in chromatin remodeling during early spermiogenesis. It has been suggested that these proteins may also participate in successful fertilization and embryo development. Using reverse transcription and real-time quantitative PCR reaction (QR-PCR) methods and zygote and embryo scoring, we compared fertilin beta, protamine 1 (PRM1), protamine 2 (PRM2), spermatid-specific linker histone 1 (HILS1) mRNAs levels, in vitro fertilization ability of mature spermatozoa, and quality of embryos obtained from in vitro fertilization (IVF). We found significantly lower contents of fertilin beta transcript in spermatozoa from patients in which IVF fertilization failed (p<0.001). We also noticed a correlation between high levels of fertilin beta and increased quality of embryos (p<0.05). We observed an increase in PRM1 and PRM2 mRNA levels in spermatozoa obtained from patients with successful in vitro fertilization versus compared to the number of these transcripts isolated from spermatozoa of patients in which in vitro fertilization failed (P<0.001), (P<0.001), respectively. We found direct correlation between PRM1 and PRM2 mRNA levels to the quality of embryos (r=0.31, P=0.012), (r=0.31, P=0.011), respectively. The differences in HILS1 mRNA contents between these two groups were not statistically significant (P>0,05). We did not observe an association between HILS1 transcript contents and quality of embryos (r=0.22, P=0.076). We suggest that fertilin beta and protamines contribute not only to successful fertilization, but may have an important impact in development of preimplantation embryos.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Depa-Martynów M,Kempisty B,Lianeri M,Jagodziński PP,Jedrzejczak Psubject
Has Abstractpub_date
2007-01-01 00:00:00pages
S79-85eissn
0239-8508issn
1897-5631pii
68/S1/2007journal_volume
45 Suppl 1pub_type
杂志文章abstract::The family of epidermal growth factor (EGF, EGFR, c-erbB-2) plays a pivotal role in gastric cancer progression, invasion and metastasizing. Helicobacter pylori infection is known to contribute significantly to the formation and progression of gastric cancer. However, the mechanisms responsible for this process have no...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0102-3
更新日期:2009-01-01 00:00:00
abstract::Coccoids (Coccinea, Coccoidea, Coccomorpha, scale insects, scales) are a highly diverse group of ectoparasitic insects. They comprise 2 subgroups: primitive archaeococcoids (= Orthezioidea sensu Koteja) and advanced neococcoids (= Coccoidea sensu Koteja). The ovaries of coccoids consist of numerous short telotrophic-m...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:
更新日期:1998-01-01 00:00:00
abstract::We report the observations made on 101 healthy non-smoking men aged 21-40 (50 from two industrial suburbs of the big city of Antwerp and 51 from Peer, a predominantly rural municipality with 14,622 inhabitants, 70 km east of Antwerp, chosen as the "control" area in spite of its intensive agriculture). Persons with kno...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::We report here about the purification of prion protein 27-30 (PrP 27-30) and scrapie-associated fibrils (SAF) from hamsters infected with the 263K strain of scrapie. Ultrastructural analysis of fractions from scrapie-infected brains revealed numerous fibrils measuring approximately 20 nm in diameter and 100-200 nm in ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:INTRODUCTION:The function and localization of actin in the nucleus have not yet been fully described. However, actin seems to be a key protein in nuclear processes interacting with chromatin and matrix proteins. The aim of the study was to evaluate the effect of controlled expression of nuclear pool of F-actin and spec...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2015.0008
更新日期:2015-01-01 00:00:00
abstract::The present paper is the first work to determine the effect of lipopolysaccharide (LPS) in the pilocarpine model of epilepsy on the morphology of rat hippocampal astrocytes in vivo. The study involved adult male Wistar rats, which 72 hours prior to administration of pilocarpine hydrochloride (PILO) were intraperitonea...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/fhc.2011.0030
更新日期:2011-01-01 00:00:00
abstract::The advent of DNA and RNA labelling techniques which allow non-autoradiographic detection of specific nucleic acid sequences has opened new possibilities for rapid diagnosis at the nucleic acid level. There are already practical applications. However, in order to fully compete with radioactive procedures an increase i...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Within the thymus gland of the European common frog, Rana temporaria, cells with endocrine- like appearance have been found. At the ultrastructural level the most characteristic feature of their cytoplasm is the presence of secretory granules. Some cells possess irregular electron lucent granules with an eccentrically...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract:INTRODUCTION:Sucla2, a β subunit of succinyl coenzyme A synthase, is located in the mitochondrial matrix. Sucla2 catalyzes the reversible synthesis of succinate and adenosine triphosphate (ATP) in the tricarboxylic acid cycle. Sucla2 expression was found to be correlated with the capacitation of boar spermatozoa. We ha...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2016.0020
更新日期:2016-01-01 00:00:00
abstract::Structural chromosome aberrations and associated segmental or chromosomal aneusomies are major causes of reproductive failure in humans. Despite the fact that carriers of reciprocal balanced translocation often have no other clinical symptoms or disease, impaired chromosome homologue pairing in meiosis and karyokinesi...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0067-2
更新日期:2009-01-01 00:00:00
abstract::The current study was inspired by the fact that since 2004 no report had appeared on the occurrence of this peptide in healthy parotid glands of humans and animals. The objective of the current study was to investigate the immunolocalization of PTHrP in the parotid gland of three male rodents: 6 common voles (Microtus...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-010-0003-5
更新日期:2010-01-01 00:00:00
abstract::Parathyroid hormone-related protein (PTHrP) was isolated from tumours and is thought to represent the main factor responsible for humoral hypercalcaemia, which accompanies neoplastic diseases. At present, the protein is known to reside in multiple tissues and organs of both humans and animals. Our study was aimed at d...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::The study included 61 patients (35 men and 26 women) ages 47 to 74 in whom a primary liver cancer was diagnosed or neoplastic metastases to the liver were confirmed in the course of a cancer of the stomach or the large bowel. In each patient the endogenous serum diazepam concentration (ESDC) was estimated chromatograp...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Using immunohistochemistry and Western blot analysis we attempted to identify the estrogen receptors in ovine luteal cells at different stages of the estrous cycle. Monoclonal antibody against estrogen receptors was used for immunolocalization of estrogen receptor-alpha in corpora lutea sections. Generally, the most i...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::In cervical cancer, glycosylation has been suggested as being involved in both its carcinogenesis and invasive capacity. In this work, we analyzed mucin type O-glycosylation in biopsies of invasive cervical cancer in FIGO stage II B through histochemistry using lectins specific for O-glycosidically linked glycans. Our...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/19748
更新日期:2012-10-08 00:00:00
abstract::The effect of a single dose of adrenaline on the neurosecretory action of the hypothalamo-hypophyseal system was investigated in 90 male rats. Increased release and transport of neurosecretory material were observed after a low dose of adrenaline, and the enhancement of the neurosecretory process was noted following a...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::The presence and pattern of coexistence of catecholamine-synthesizing enzymes and some neuropeptides in nerve fibres supplying thoraco-cranial arteries of the sexually immature gilts were investigated in whole mount preparations. The studied substances included: tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (D ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Exposure to glucocorticoids (GCs) leads to numerous changes in various biological systems including the reproductive system. The aim of the present study was to find out whether dexamethasone (Dex), a widely used GC, would influence the apoptosis and expression of Bax, an important proapoptotic protein, in the mouse t...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0041-z
更新日期:2009-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/fhc.2011.0097
更新日期:2011-01-01 00:00:00
abstract::Neuroblastoma represents one of the most frequently developing malignant solid tumours in children. At the time of diagnosis, in more than half of the cases, metastatic cells are also present in the bone marrow. The present study was aimed at immunocytochemical analysis of selected neuropeptide manifestation in metast...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.2014.0005
更新日期:2014-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Mucins have been shown to be aberrantly overexpressed in various diseases including cystic fibrosis, asthma, and cancer. Recent studies have uncovered the roles of these mucins in the pathogenesis of cancer. The presence of MUC-1 has also been detected on the cell surface of multiple myeloma (MM) cells in peripheral b...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/fhc.2011.0089
更新日期:2011-01-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is an endocrinological and metabolic disorder which may concern about 3-8% of women. Some PCOS women have the increased leptin concentration in blood serum. Leptin concentration is higher in patients with high body mass index (BMI) and impaired tissue sensitivity to insulin. The aim of...
journal_title:Folia histochemica et cytobiologica
pub_type: 临床试验,杂志文章
doi:10.2478/v10042-009-0032-0
更新日期:2009-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Recently identified biological risk factors in B-cell chronic lymphocytic leukemia (B-CLL) include ZAP-70 and CD38 expression. The present study was conducted to clarify whether a combined analysis could improve predictive impact of these two parameters. We examined the expression of ZAP-70 and CD38 by flow cytometry ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-008-0022-7
更新日期:2008-01-01 00:00:00
abstract::Mice--BDF, hybrides, males aged 3 months were injected intraperitoneally with 10(4) lymphatic leukemia cells P 388. The mice were killed by bleeding after 2.5 and 10 days after the injection. Gamma-glutamyltransferase (GGT), leucylaminopeptidase (LAP) and cobalt activated acylase activity were determined in the serum ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Tyrosine phosphorylation of numerous proteins is one of the earliest events detectable during Fcgamma receptor-mediated phagocytosis. We demonstrate that IgG-coated particles associated with the surface of macrophages are enriched with numerous tyrosine-phosphorylated proteins. During particle internalization the prot...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::The study has been carried out on Wistar rats. The animals were divided into two groups: the control rats received 0.9% saline solution intraperitoneally in there consecutive days. The experimental animals were administered 1% solution of acetylphenylhydrazine (APHZ) intraperitoneally also in three days. Prior to the ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00