Abstract:
:Exposure to glucocorticoids (GCs) leads to numerous changes in various biological systems including the reproductive system. The aim of the present study was to find out whether dexamethasone (Dex), a widely used GC, would influence the apoptosis and expression of Bax, an important proapoptotic protein, in the mouse testicular germ cells. Experimental groups of 8 male NMRI mice received one of the following treatments daily for 7 days: 4, 7 and 10 mg/kg Dex. Control groups were treated with equivalent volumes of saline. Experimental and control animals were sacrificed 24 h after the last injection. Immunohistochemical procedure was used to evaluation of Bax expression and the deoxyuridine nick-end labeling (TUNEL) was applied to assessment of the apoptotic germ cells. Bax expression was upregulated mainly at stages VII-VIII of spermatogenic cycle (p<0.05) in experimental groups. Apoptotic index was significantly increased in 7 and 10 mg/kg Dex treated mice (p<0.05). It appears that GCs such as Dex could induce apoptosis through the expression of proapoptotic proteins.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Mahmoud H,Mahmoud O,Layasadat K,Naeim Adoi
10.2478/v10042-009-0041-zsubject
Has Abstractpub_date
2009-01-01 00:00:00pages
237-41issue
2eissn
0239-8508issn
1897-5631pii
GU08264270P7246Ujournal_volume
47pub_type
杂志文章abstract::Progress in recognition of structure and function in a variety of cells has always been linked to development of new research techniques. Beyond doubt, immunocytochemical techniques belong to such new methods. However, the techniques had not been widely applied to electron microscopy until colloidal gold was introduce...
journal_title:Folia histochemica et cytobiologica
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