Abstract:
:The fibroblast growth factors (FGFs) are multifunctional proteins that, among other roles, regulate structural reorganization of uterine and placental vascular bed during pregnancy. Thus, we analyzed mRNA and protein expression and immunohistochemical localization of FGF-1 and FGF-2, and their receptors (FGFR-1 and FGFR-2) in the developing umbilical cord (UC) on days 40, 60, 75 and 90 of pregnancy and after the physiological delivery in the pig (day 114). qPCR analysis demonstrated an increase in FGF-1 and FGF-2 mRNA levels beginning on day 75 and on day 114 of pregnancy, respectively. In addition, significantly increased FGFR-1IIIc mRNA expression was also found on day 114. On the other hand, no significant changes in FGFR-2IIIb mRNA expression were observed. Western Blot analysis revealed a decrease in FGF-1 and FGFR-2 protein expression after day 40. Beside an increased protein expression of FGF-2 on day 60, no significant changes in FGFR-1 protein expression were detected. Immunohistochemical staining enabled detection of FGF-FGFR system, with different intensity of immunoreaction in endothelial and tunica media cells of the umbilical vessels and in allantoic duct and amniotic epithelium as well as in myofibroblasts. In conclusion, our results show that members of FGF-FGFR system are expressed specifically in UC structures. Furthermore their day of pregnancy-related expression suggest that they may be an important players during UC formation and development.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Chrusciel M,Rekawiecki R,Ziecik AJ,Andronowska Adoi
10.2478/v10042-010-0073-4subject
Has Abstractpub_date
2010-12-01 00:00:00pages
572-80issue
4eissn
0239-8508issn
1897-5631pii
2P62621X53402200journal_volume
48pub_type
杂志文章abstract:INTRODUCTION:In traditional medicine, Citrullus colocynthis is used to treat diabetes, hyperlipidemia, cardiovascular diseases, inflammation, and oxidative stress, all of which can appear when a diet rich in vegetable fats, such as palm oil, is continuously consumed. Such high-fat diets are chronic stressors of the hyp...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2017.0011
更新日期:2017-01-01 00:00:00
abstract::The autoradiographic method was used to compare the 3H-uridine incorporation and the number of hybridization sites with 3H-5S DNA in yellow lupin root apical meristem, root hair of uninfected roots as well as in root nodule cortex and bacteriod-containing tissue. It has been shown that the number of hybridization site...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:INTRODUCTION:Adipogenesis comprises multiple processes by which mesenchymal stem cells differentiate into adipocytes. To increase our knowledge of the mechanism underlying adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs), we performed full-genome gene expression microarray and gene ontolog...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2016.0003
更新日期:2016-01-01 00:00:00
abstract::Tuberculosis (TB) still remains the leading cause of mortality due to bacterial pathogen. The only currently available vaccine against TB, Bacille Calmette-Guérin (BCG) is at best credited with a 50% overall protective efficacy. Skin testing with purified protein derivative (PPD) from Mycobacterium tuberculosis is the...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-008-0044-1
更新日期:2008-01-01 00:00:00
abstract:INTRODUCTION:Chronic rhinosinusitis (CRS) affects 14% of the world population. The high motility group box 1 (HMGB1) protein triggers inflammation, cell proliferation and cell survival through its receptor for advanced glycation end products (RAGE) upon release from stressed or necrotic cells. The aim of the study was ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2015.0007
更新日期:2015-01-01 00:00:00
abstract::On the basis of radioactivity of interphase cells in the antheridial filaments of Chara vulgaris after a 20 min. incubation with 3H-uridine and after a 40 min. postincubation the activity of synthesis and transport of newly synthesized RNA has been studied in different-sized nucleoli. Investigations of their ultrastru...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Exposure to glucocorticoids (GCs) leads to numerous changes in various biological systems including the reproductive system. The aim of the present study was to find out whether dexamethasone (Dex), a widely used GC, would influence the apoptosis and expression of Bax, an important proapoptotic protein, in the mouse t...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0041-z
更新日期:2009-01-01 00:00:00
abstract::The present study deals with immunohistochemical localization of S-100 protein in mouse, bank vole and pine vole testis and epididymis. S-100 protein immunoreactivity was observed in the endothelia of capillaries and lymphatic sinusoids of pine vole testis. A reaction to S-100 protein of the same intensity as that not...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Immunohistochemical localization of androgen receptor (AR) was investigated in the rat oocytes during their development in the primary, secondary and antral follicles. The group of experimental Wistar rats included prepubertal female rats, killed at 30 days of age, and mature female rats killed at estrus or metestrus....
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::After dmPGE2 treatment, both alone or together with streptozotocin, the lower, statistically significant total PL contents (in mumoles P per mg of protein) in rat liver Golgi-rich membrane fractions were found. In these groups of investigated rats, the percentage of PE+PA were statistically significantly lower than in...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::The purpose of the present study was to evaluate morphologically the response of feline teeth pulp to direct pulp capping with two different self-etch adhesive systems. Twenty-four cavities in feline teeth were mechanically exposed and assigned to one of two experimental groups: AdheSE + Tetric Ceram (the ASE group), ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/20325
更新日期:2012-01-01 00:00:00
abstract::Colorectal cancer (CRC) is one of the most common cancers of the gastrointestinal tract and the fourth cause of cancers death in the world. Soluble adhesion molecules (CAMs) are thought to have an important role in host defense against carcinogenesis. They are biomarkers of inflammation and indicators of the immune re...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0077-0
更新日期:2009-01-01 00:00:00
abstract::Histochemical and immunohistochemical methods should often but not always complement standardized histopathologic procedures. Here, we illustrate use of these ancillary techniques in a report of scrotal leiomyosarcoma. 62-year-old male patient presented with a palpable, subcutaneous 2,5 cm wide tumor arising from dart...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.2013.0046
更新日期:2013-01-01 00:00:00
abstract:INTRODUCTION:Sucla2, a β subunit of succinyl coenzyme A synthase, is located in the mitochondrial matrix. Sucla2 catalyzes the reversible synthesis of succinate and adenosine triphosphate (ATP) in the tricarboxylic acid cycle. Sucla2 expression was found to be correlated with the capacitation of boar spermatozoa. We ha...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2016.0020
更新日期:2016-01-01 00:00:00
abstract::The K-ras gene is frequently mutated in colorectal cancer and has been associated with tumor initiation and progression; approximately 90% of the activating mutations are found in codons 12 and 13 of exon 1 and just under 5% in codon 61 located in exon 2. These mutations determine single aminoacidic substitutions in t...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/fhc.2011.0097
更新日期:2011-01-01 00:00:00
abstract::The recurrent goiter is the regrowth of thyroid tissue after thyroidectomy. An inadequate surgical removal of the thyroid gland, lack of substitution therapy and pathological stimulation of the thyroid growth can all promote the recurrence. The aim of this study was to find the connection between the histopathological...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-010-0036-9
更新日期:2010-09-30 00:00:00
abstract::Factors controlling complement activation appear to exert a protective effect on pregnancy. This is particularly important in women with thrombophilia. The aim of this study was to determine the transcript and protein levels of complement decay-accelerating factor (DAF) and membrane cofactor protein (MCP) in the place...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/19758
更新日期:2012-10-08 00:00:00
abstract::The most important features that determine the vital role of bone include: a) a continuous supply of calcium, which is indispensible for every cell of the entire organism at all times, and b) the delivery of circulating blood cells and some adult stem cells to keep the body vigorous, ready for self-reparation, and con...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.5603/fhc.2011.0079
更新日期:2011-01-01 00:00:00
abstract::We have investigated immunohistochemically the effect of dl-alpha-tocopherol (vitamin E) on thyroid gland with 6-n-propyl-2-thiouracil (PTU)-induced hypothyroidism in rats. The animals were divided into four groups. Rats in group I were designated as control, rats in group II were treated with injections of PTU (10 mg...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:INTRODUCTION:The function and localization of actin in the nucleus have not yet been fully described. However, actin seems to be a key protein in nuclear processes interacting with chromatin and matrix proteins. The aim of the study was to evaluate the effect of controlled expression of nuclear pool of F-actin and spec...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2015.0008
更新日期:2015-01-01 00:00:00
abstract:INTRODUCTION:Currently, elderly people constitute a large proportion of patients undergoing coronary artery bypass grafting (CABG). Activated smooth muscle cells in the tunica media of saphenous vein (SV) grafts are thought to play a key role in the formation of neointima and development of occluding atherosclerotic pl...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2016.0011
更新日期:2016-01-01 00:00:00
abstract::Calcium binding sites were revealed in the polytropic egg vesicles of Galleria mellonella (Lepidoptera) by fixation of the ovariole with glutaraldehyde in the presence of excess of calcium. Calcium dependent deposits appeared in the mitochondrial matrix in cells of the epithelial sheath and of the follicular epitheliu...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::The study was undertaken to determine the immunoexpression of protein products of nm23 genes which are thought to be potential metastasis suppressor genes, in esophageal squamous cell carcinoma, and to analyze their relationship to selected clinicopathological features (age, sex, tumour size, depth of invasion, presen...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The goal of our study was to assess suitability of FNA biopsy material as a source of samples (cell suspension) for DNA ploidy assessment in neoplastic tumors using flow cytometry. DNA ploidy is an established prognostic factor in many types of cancers. Aneuploid breast tumors are characterized by increased aggressive...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract:INTRODUCTION:Aging causes morphological and functional changes in the thyroid gland. Free radicals play a key role in the pathology of normal aging. Vimentin and cytokeratin are cytoskeletal intermediate filaments that are often used as indirect indices of tissue injury. The aim of the study was to clarify the age-rela...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2018.0015
更新日期:2018-01-01 00:00:00
abstract::Three proteinases from the azurophilic granules of horse leucocytes are typical elastases degrading elastin at neutral pH. Both proteinases: 1 and 2A exhibit similar elastinolytic activity, comparable with human leucocyte elastase (HLE). In relation to human enzyme, elastase 2B shows several-fold higher activity, whic...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Light microscopic histochemical studies to visualize semicarbazide-sensitive and H2O2-generating amine oxidase (SSAOX, benzylamine oxidase, BAOX; EC 1.4.3.6?) are usually performed with the coupled peroxidatic oxidation technique of Ryder et al. [25]. For methodological reasons this procedure has its limitations and w...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Two hypotheses explain the role of adult progenitor cells in myocardial regeneration. Stem cell plasticity which involves mobilization of stem cells from the bone marrow and other niches, homing to the area of tissue injury and transdifferentiation into functional cardiomyocytes. Alternative hypothesis is based on the...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract::T cell memory in comparison with B cell memory is not well understood. This review focuses on CD8+ and CD4+ memory T cells. In this article we try to define memory cells and also present models of memory T cells formation. We would also like to delineate their differentiation into distinct subsets. Long-lived memory T...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.2478/v10042-008-0077-5
更新日期:2008-01-01 00:00:00
abstract::The Ubiquitin-Proteasomes System (UPS) and autophagy, two main intracellular protein degradation pathways within the eukaryotic cells which were originally regarded as rather independent, seem to be very closely related. Proteasome inhibitors, including the multipathway inhibitor bortezomib, are drawing increased atte...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.5603/FHC.2013.0036
更新日期:2013-01-01 00:00:00