Abstract:
INTRODUCTION:Adipogenesis comprises multiple processes by which mesenchymal stem cells differentiate into adipocytes. To increase our knowledge of the mechanism underlying adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs), we performed full-genome gene expression microarray and gene ontology analyses of induced differentiation of hMSCs. MATERIAL AND METHODS:Adipogenic differentiation of hMSCs was induced by an adipogenic medium, and total RNA was extracted from undifferentiated hMSCs (day 0) and differentiated adipocytes (day 14). Then microarray hybridization of RNA samples was performed. The GeneChip Operating Software was used to analyze the hybridization data to identify differentially expressed genes, which were performed Gene Ontology categorization and pathway analysis. Pathway-act-network and genes-act-network were built according to the Kyoto Encyclopedia of Genes and Genomes database. Some differentially expressed genes were subjected to qRT-PCR to verify the microarray data. RESULTS:We detected a total of 3,821 differentially expressed genes, of which 753 were upregulated and 3,068 downregulated. These genes were well represented in a variety of functional categories, including collagen fibril organization, brown fat cell differentiation, cell division, and S phase of mitotic cell cycle. Subsequently, pathway analysis was conducted, and significant pathways (from top 50) were selected for pathway-act-network analysis, which indicated that the mitogen-activated protein kinase (MAPK) pathway and cell cycle were of high degrees (> 10). Gene-act-network analysis showed that insulin-like growth factor 1 receptor (IGF1R), histone deacetylase 1 (HDAC1), HDAC2, MAPK13, MAPK8, phosphoinositide-3-kinase regulatory subunit 1 (PI3KR1), and PI3KR2 also had high degrees (> 18). CONCLUSIONS:Collectively, these data provide novel information and could serve as a basis for future study to clarify the mechanisms underlying adipocyte differentiation of hMSCs.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Xu X,Li X,Yan R,Jiang H,Wang T,Fan L,Wu J,Cao J,Li Wdoi
10.5603/FHC.a2016.0003subject
Has Abstractpub_date
2016-01-01 00:00:00pages
14-24issue
1eissn
0239-8508issn
1897-5631pii
VM/OJS/J/43734journal_volume
54pub_type
杂志文章abstract:INTRODUCTION:Aging causes morphological and functional changes in the thyroid gland. Free radicals play a key role in the pathology of normal aging. Vimentin and cytokeratin are cytoskeletal intermediate filaments that are often used as indirect indices of tissue injury. The aim of the study was to clarify the age-rela...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2018.0015
更新日期:2018-01-01 00:00:00
abstract::Microtubules present in the epidermis of Ornithogalum umbellatum ovary in the area of lipotubuloids (i.e. aggregates of lipid bodies surrounded by microtubules) are 25-51 nm in diameter. They consist mainly of 10 and 11, sometimes 9 and 12 protofilaments. An average diameter of microtubule consisting of 9 subunits is ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::A literature survey was performed of the methods of quantitative assessment of the activity and concentration of cathepsin D and its inhibitors. Usefulness of non-modified and modified proteins and synthetic peptides as measurement substrates was evaluated. The survey includes also chemical and immunochemical methods ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.2478/v10042-009-0073-4
更新日期:2009-01-01 00:00:00
abstract::Diplotene oocyte nucleus of the scorpionfly Panorpa communis is transcriptionally silent and contains numerous nuclear bodies including interchromatin granule clusters (IGCs). The latter consist of the granules of 30-50 nm in diameter and contain IGC marker protein SC35 as well as RNA polymerase II. In this study, we ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0006-2
更新日期:2009-01-01 00:00:00
abstract::Recently, the potential risk of malignant cancer development in psoriatic patients has been highlighted. However, the relationship between the therapeutic schemes in psoriasis and possible neoplastic transformation has not been so far clearly explained. Phototherapy is considered a very effective therapeutic method in...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.5603/17843
更新日期:2012-01-01 00:00:00
abstract::The effect of the NSP fraction and its four peptide constituents (A, C2, D and G) on the 3H-thymidine incorporation into REF and R-XC cells in vitro was described. Only peptide G (at concentration 5 micrograms/ml) affected the growth of the investigated cells, decreasing the 3H-thymidine uptake to 4-13% relative to un...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract:INTRODUCTION:Fascins are a group of proteins taking part in the maintenance of a proper structure of the cellular cytoskeleton. Fascin-1 is an actin-bundling protein present in neurons, fibroblasts, endothelial, smooth muscle, dendritic and mesenchymal cells whereas lack of its expression is characteristic of epithelia...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2018.0011
更新日期:2018-01-01 00:00:00
abstract::The study has been carried out on Wistar rats. The animals were divided into two groups: the control rats received 0.9% saline solution intraperitoneally in there consecutive days. The experimental animals were administered 1% solution of acetylphenylhydrazine (APHZ) intraperitoneally also in three days. Prior to the ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Embryo implantation consists of a series of events promoting the invasion of the endometrium and then the uterine arterial system by the extra-embryonic trophoblast. In order for this semi-heterologous implantation to succeed, the endometrium has to first undergo a number of structural and biochemical changes (decidua...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0058-3
更新日期:2009-01-01 00:00:00
abstract::Dendritic cells (DCs) are regarded as the most potent antigen presenting cells that are well suited to activate T cells toward various antigens, such as tumor-associated antigens, due to their costimulatory activity. There is evidence that DCs are of diverse origin, with at least two types of myeloid and lymphoid prec...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is an endocrinological and metabolic disorder which may concern about 3-8% of women. Some PCOS women have the increased leptin concentration in blood serum. Leptin concentration is higher in patients with high body mass index (BMI) and impaired tissue sensitivity to insulin. The aim of...
journal_title:Folia histochemica et cytobiologica
pub_type: 临床试验,杂志文章
doi:10.2478/v10042-009-0032-0
更新日期:2009-01-01 00:00:00
abstract::The level of transglutaminase activity has been investigated by the fluorescence method in peritoneal macrophages of control and transplantable melanomas bearing hamsters. If the transglutaminase activity is concerned the macrophages in no one of the groups examined were a homogenous population. Differences in macroph...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Combined (3H) estradiol autoradiography and oxytocin immunocytochemistry were used in order to study co-localization of cytoplasmic oxytocin immunoreactivity and nuclear uptake of (3H) estradiol in the forebrain of adult ovariectomized mice. Labelling with (3H) estradiol was found in subpopulations of neurons that con...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::The structure of epidermis and appearance of keratinocytes is described in intact skin specimens from human corpses stored after death under refrigeration. Two groups of alterations can be identified depending on the epidermal layer. In the spinous layer, the cells are characterized by crescent-shaped nuclei surrounde...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Therapeutic outcomes of definitively treated non-small-cell lung cancer (NSCLC) are unacceptably poor. It has been proposed that the manipulation of dendritic cells (DCs) as a "natural" vaccine adjuvant may prove to be a particularly effective way to stimulate antitumor immunity. Presently, there is no standardized me...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-008-0078-4
更新日期:2008-01-01 00:00:00
abstract::The localization of estrogen receptor alpha (ERalpha) in the ovaries of postmenopausal women is a very up-to-date topic in the aspect of using estrogens therapy in the clinical situations of different type. In ovaries of reproductive age women ERalpha is present in ovary stroma, theca and granulosa cells, ovary surfac...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::The autoradiographic method was used to compare the 3H-uridine incorporation and the number of hybridization sites with 3H-5S DNA in yellow lupin root apical meristem, root hair of uninfected roots as well as in root nodule cortex and bacteriod-containing tissue. It has been shown that the number of hybridization site...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Early recognition of prostate cancer (PC) based on biological markers could be helpful in identification of differences in benign and malignant lesions and facilitate further precise indication for more aggressive treatment. Therefore, the aim of our study was to assess expression of hTERT (human telomerase reverse tr...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.2013.0010
更新日期:2013-01-01 00:00:00
abstract:INTRODUCTION:Liquid biopsies are noninvasive tests using blood or body fluids to detect circulating tumor cells (CTCs) or the products of tumor cells, such as fragments of nucleic acids or proteins that are shed into biological fluids from primary tumor or its metastates. The analysis of published clinical studies prov...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2017.0005
更新日期:2017-01-01 00:00:00
abstract::The frequency of Helicobacter pylori infection in population can depend on the organism resistance, genetic condition, and bacterial strains virulence. A vacA gene, of mosaic structure, which encodes vacuolating cytotoxin is one of the known genes of H. pylori. The existence of several different genotypes of s and m r...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::A literature survey was performed of human cathepsin D gene, cathepsin D biosynthesis, posttranslatory modifications, transport within the cell, substrate specificity and catalytic effect. Methods used to determine the activity and level of this proteinase as well as its role in the biochemistry and pathobiochemistry ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.2478/v10042-008-0003-x
更新日期:2008-01-01 00:00:00
abstract::The activity of delta (5)3 beta-hydroxysteroid dehydrogenase was examined histochemically in 6 to 10 days aged horse blastocysts. A positive reaction was noted in the blastomeres of all embryos incubated in medium with substrate. Measurable amounts of progesterone, androgens and estrogens were found in blastocysts on ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Platinum-based chemotherapy is the main type of I-line treatment of advanced and non-operative NSCLC patients without EGFR gene mutation. The excision repair cross-complementation group 1 (ERCC1) is an enzyme that executes the incision of the damaged DNA strand and removes platinum-induced DNA adducts. We investigated...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/18700
更新日期:2012-04-24 00:00:00
abstract::Nuclear bodies (NBs) are intranuclear structures described in normal and pathologically altered cells of humans and animals. The NBs are 0.3-1.5 microns round structures. Their function is unknown. In the present paper, we describe NBs in the nuclei of the conjunctiva epithelial cells in one patient with cicatricial p...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The presence and pattern of coexistence of catecholamine-synthesizing enzymes and some neuropeptides in nerve fibres supplying thoraco-cranial arteries of the sexually immature gilts were investigated in whole mount preparations. The studied substances included: tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (D ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::In cases of decomposed bodies Y chromosomal STR markers may be useful in identification of a male relative. The authors assessed typeability PowerPlex Y (Promega) loci in tissue material stored in water and soil environment. Tissue material was collected during autopsies of five persons aged 20-30 years with time of d...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::The study aimed at examination of tissue expression of polysaccharides and secretory mucin 5AC (MUC5AC) in young patients (up to 25 years of age) with a symptomatic gallstones. For comparison, patients most frequently subjected to cholecystectomy were studied, i.e. patients of approximately 50 years of age with the sa...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-010-0053-8
更新日期:2010-12-01 00:00:00
abstract::The present study deals with immunohistochemical localization of PTHrP in European bison and pine vole testis and epididymis. PTHrP immunoreactivity was observed in spermatogenic cells of seminiferous tubules in European bison and pine vole testis, with the strongerst reaction occurring in spermatozoa of pine vole tes...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract:INTRODUCTION:Osteoarthritis (OA) is a severe joint degeneration disease in elderly people described by the advanced degradation of articular cartilage, which ultimately leads to chronic pain. Trans-cinnamaldehyde (TCA) exerted its anti-inflammatory function in numerous disease syndromes; however, its role in the pathog...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2020.0025
更新日期:2020-01-01 00:00:00
abstract::Within the thymus gland of the European common frog, Rana temporaria, cells with endocrine- like appearance have been found. At the ultrastructural level the most characteristic feature of their cytoplasm is the presence of secretory granules. Some cells possess irregular electron lucent granules with an eccentrically...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00