Abstract：
INTRODUCTION:Liquid biopsies are noninvasive tests using blood or body fluids to detect circulating tumor cells (CTCs) or the products of tumor cells, such as fragments of nucleic acids or proteins that are shed into biological fluids from primary tumor or its metastates. The analysis of published clinical studies provides coherent evidence that the presence of CTCs detected in peripheral blood is a strong prognostic factor in patients with colorectal carcinoma (CRC). The aim of the study was to implement size-based separation protocol of CTCs in CRC patients. MATERIAL AND METHODS:Patients diagnosed with different stages of CRC (n = 98) were included in the study. All patients have been diagnosed for colorectal adenocarcinoma by pathology examination, 45 patients with colon carcinoma and 53 with rectosigmoid cancer. A size-based separation method (MetaCell®) for viable CTC enrichment from peripheral blood was used to assess the presence of CTCs by cytomorphological evaluation using vital fluorescence microscopy. RESULTS:Cytomorphological analysis revealed that 81 (83%) tested samples were CTC-positive and 17 (17%) were CTC-negative. We report a successful isolation of CTCs with proliferation potential in patients with CRC. The CTCs were cultured in vitro for further downstream applications. Some of the isolated CTCs were able to grow in vitro for 6 months as a standard cell culture. CONCLUSIONS:We established a reliable, inexpensive and relatively fast protocol for CTCs enrichment in CRC patients by means of vital fluorescence staining which enables their further analysis in vitro.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Eliasova P,Pinkas M,Kolostova K,Gurlich R,Bobek Vdoi
10.5603/FHC.a2017.0005subject
Has Abstractpub_date
2017-01-01 00:00:00pages
1-5issue
1eissn
0239-8508issn
1897-5631pii
VM/OJS/J/49220journal_volume
55pub_type
杂志文章abstract：:The fibroblast growth factors (FGFs) are multifunctional proteins that, among other roles, regulate structural reorganization of uterine and placental vascular bed during pregnancy. Thus, we analyzed mRNA and protein expression and immunohistochemical localization of FGF-1 and FGF-2, and their receptors (FGFR-1 and FG...
journal_title：Folia histochemica et cytobiologica
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journal_title：Folia histochemica et cytobiologica
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journal_title：Folia histochemica et cytobiologica
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doi：10.2478/v10042-08-0107-3
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abstract：:Forty pituitary adenomas were immunostained with an antibody raised against the C-terminal fragment (101-109) of human prothymosin alpha (PT alpha). The strong positive immunostaining was found in the subpopulation of cell nuclei and intratumoral vessel walls, while the cytoplasm of adenoma cells was slightly immunopo...
journal_title：Folia histochemica et cytobiologica
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journal_title：Folia histochemica et cytobiologica
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abstract：:Immunohistochemical localization of androgen receptor (AR) was investigated in the rat oocytes during their development in the primary, secondary and antral follicles. The group of experimental Wistar rats included prepubertal female rats, killed at 30 days of age, and mature female rats killed at estrus or metestrus....
journal_title：Folia histochemica et cytobiologica
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journal_title：Folia histochemica et cytobiologica
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journal_title：Folia histochemica et cytobiologica
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journal_title：Folia histochemica et cytobiologica
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0069
更新日期：2011-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
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journal_title：Folia histochemica et cytobiologica
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doi：
更新日期：2007-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
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更新日期：2012-10-08 00:00:00
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journal_title：Folia histochemica et cytobiologica
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更新日期：2009-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
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doi：
更新日期：2003-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
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更新日期：2009-01-01 00:00:00
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journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0097
更新日期：2011-01-01 00:00:00
