Abstract：
:Histochemical and immunohistochemical methods should often but not always complement standardized histopathologic procedures. Here, we illustrate use of these ancillary techniques in a report of scrotal leiomyosarcoma. 62-year-old male patient presented with a palpable, subcutaneous 2,5 cm wide tumor arising from dartos muscle. The tumor was diagnosed leiomyosarcoma G2 pT1b. Interestingly, the sarcomatous mass was focally strictly attached to convoluted, benign bundles of smooth muscles that were intermingled with tumor mass at peripheral, lateral and superior sides of the lesion. We have used immune- and histochemical methods to confirm histopathological findings based on H&E staining. As expected, in tumor cells smooth muscle actin and desmin were strongly immunopositive similarly as Masson trichrome staining, while S100 and CD34 antigens were immunonegative except for sustained positivity for CD34 in vessels. The auxiliary staining methods can provide additional information on the tumorigenesis of leiomyosarcoma. They can also serve to determine additional features of prognostic significance, since e.g. immunoreactivity of CD34 accurately maps vascular density of tumor and enables a careful assessment of vascular invasion in course of leiomyosarcoma as well.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Wincewicz A,Lewitowicz P,Sulkowska U,Sulkowski S,Horecka-Lewitowicz A,Koziel D,Gluszek Sdoi
10.5603/FHC.2013.0046subject
Has Abstractpub_date
2013-01-01 00:00:00pages
339-42issue
4eissn
0239-8508issn
1897-5631pii
VM/OJS/J/37109journal_volume
51pub_type
杂志文章abstract：:The increased ambient temperature affects the function of hypothalamic-pituitary-adrenal (HPA) axis. Since the correlation among vasopressin (VP), adrenocorticotropic hormone (ACTH) and corticosterone (CORT) responses to various stressors have been long recognized, the aim of this study was to reveal the aforementione...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-010-0071-6
更新日期：2010-12-01 00:00:00
abstract：:A simple chemically defined medium is described which in primary cultures promotes differentiation of cells form embryonic chicken breast muscle to myotubes and cross-striated myofibres. It is shown that 5 minute pulse treatment of the myogenic cells with the insulin containing solution is sufficient to switch on, in ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Angiogenesis represents an important process manifested in tumour growth and in development of metastases. Using immunohistochemistry, the authors evaluated number of vessels in various nodular lesions of the thyroid (54 cases). Expression of CD34 antigen and microvessel density were evaluated in sections of archival ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Animals with experimental uremia, which underwent thyroparathyroidectomy, reveal numerous metabolic disorders that can influence morphology and activity of endocrine cells of the scattered neuroendocrine system. The aim of the study was the evaluation of the influence of thyroparathyroidectomy in rats with chronic ren...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Within the thymus gland of the European common frog, Rana temporaria, cells with endocrine- like appearance have been found. At the ultrastructural level the most characteristic feature of their cytoplasm is the presence of secretory granules. Some cells possess irregular electron lucent granules with an eccentrically...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Factors controlling complement activation appear to exert a protective effect on pregnancy. This is particularly important in women with thrombophilia. The aim of this study was to determine the transcript and protein levels of complement decay-accelerating factor (DAF) and membrane cofactor protein (MCP) in the place...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/19758
更新日期：2012-10-08 00:00:00
abstract：:Preterm labour and prematurity are still a main cause of perinatal morbidity nowadays. The aim of our study was to assess the role of MMP-8 as a predictive marker of preterm delivery. Four groups of patients were involved to the study: I - pregnant women at 24-34 weeks of gestation with any symptoms of threatened pret...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-010-0037-8
更新日期：2010-09-30 00:00:00
abstract：:The effect of limited amino acid nutrition upon the growth of B16 melanoma cells was investigated. It was observed that the cells in Basal Medium Eagle do not proliferate and are inhibited predominantly in the G1 phase of cell cycle. The cells which were inhibited in growth for 7 or 12 days in Basal Medium Eagle were ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：INTRODUCTION:Sucla2, a β subunit of succinyl coenzyme A synthase, is located in the mitochondrial matrix. Sucla2 catalyzes the reversible synthesis of succinate and adenosine triphosphate (ATP) in the tricarboxylic acid cycle. Sucla2 expression was found to be correlated with the capacitation of boar spermatozoa. We ha...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2016.0020
更新日期：2016-01-01 00:00:00
abstract：:Differentiation of the lingual taste discs (TDs) was studied in tadpoles of Rana esculenta, Hyla arborea and Pelobatesfuscus by means of LM, TEM, and SEM. The process of differentiation of mucous cells (MCs) within a TD anlage was investigated subsequently from 39th to 46th Gosner's developmental stage. In R. esculent...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The structure of epidermis and appearance of keratinocytes is described in intact skin specimens from human corpses stored after death under refrigeration. Two groups of alterations can be identified depending on the epidermal layer. In the spinous layer, the cells are characterized by crescent-shaped nuclei surrounde...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The most important features that determine the vital role of bone include: a) a continuous supply of calcium, which is indispensible for every cell of the entire organism at all times, and b) the delivery of circulating blood cells and some adult stem cells to keep the body vigorous, ready for self-reparation, and con...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：10.5603/fhc.2011.0079
更新日期：2011-01-01 00:00:00
abstract：:Nitric oxide (NO) is produced by NO synthase (NOS) isoforms: neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS). It is believed that, while nNOS and eNOS are effective in regulation of normal physiological processes, iNOS is expressed at an increasing rate especially in inflammatory process. The aim ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0032-5
更新日期：2008-01-01 00:00:00
abstract：:Morphology and some molecular aspects of hyperplastic (bronchial basal cell hyperplasia and alveolar cell hyperplasia), metaplastic (squamous metaplasia), preneoplastic and early neoplastic (dysplasia in squamous metaplasia, cancer in situ and atypical alveolar cell hyperplasia) changes were studied in 180 lungs resec...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Three proteinases from the azurophilic granules of horse leucocytes are typical elastases degrading elastin at neutral pH. Both proteinases: 1 and 2A exhibit similar elastinolytic activity, comparable with human leucocyte elastase (HLE). In relation to human enzyme, elastase 2B shows several-fold higher activity, whic...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Double fluorescence staining with o-phthalaldehyde (OPT) and propidium diiodide (PI) was applied to measure the nuclear protein SH/DNA ratio in species differing in 2C DNA content. It has been shown that protein SH/DNA ratio decreases along with the increase in 2C DNA content. In endosperm, this ratio is higher than i...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Parathyroid hormone-related protein (PTHrP) was isolated from tumours and is thought to represent the main factor responsible for humoral hypercalcaemia, which accompanies neoplastic diseases. At present, the protein is known to reside in multiple tissues and organs of both humans and animals. Our study was aimed at d...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The family of epidermal growth factor (EGF, EGFR, c-erbB-2) plays a pivotal role in gastric cancer progression, invasion and metastasizing. Helicobacter pylori infection is known to contribute significantly to the formation and progression of gastric cancer. However, the mechanisms responsible for this process have no...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0102-3
更新日期：2009-01-01 00:00:00
abstract：:The present study was aimed at disclosing axotomy- and inflammation-induced changes in the chemical coding of retrogradely labelled distal bowel-projecting neurons in the porcine IMG. Particular attention was paid to the changes in the expression pattern of vasoactive intestinal polypeptide and nitric oxide synthase (...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：INTRODUCTION:Aging causes morphological and functional changes in the thyroid gland. Free radicals play a key role in the pathology of normal aging. Vimentin and cytokeratin are cytoskeletal intermediate filaments that are often used as indirect indices of tissue injury. The aim of the study was to clarify the age-rela...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2018.0015
更新日期：2018-01-01 00:00:00
abstract：:The ultrastructural localization of a proteasomal antigen in human spermatozoa was studied by means of immunolabeling with the MPC21 monoclonal antibody and secondary gold labeled antibody with 1.4 nm gold particles in combination with silver enhancement reaction using pre-embedding technique. The labeling was found i...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Neuroblastoma represents one of the most frequently developing malignant solid tumours in children. At the time of diagnosis, in more than half of the cases, metastatic cells are also present in the bone marrow. The present study was aimed at immunocytochemical analysis of selected neuropeptide manifestation in metast...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The influence of bilateral destruction of dopaminergic endings in the anterior and in the posterior part of nucleus accumbens (NAS) and in the nucleus septi lateralis (NSL), by 6-hydroxydopamine (6-OHDA) infusions, on the facilitatory effect of cholecystokinin-unsulfated octapeptide (CCK-8US) and caerulein (CER) on me...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Forty pituitary adenomas were immunostained with an antibody raised against the C-terminal fragment (101-109) of human prothymosin alpha (PT alpha). The strong positive immunostaining was found in the subpopulation of cell nuclei and intratumoral vessel walls, while the cytoplasm of adenoma cells was slightly immunopo...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0096-2
更新日期：2009-01-01 00:00:00
abstract：:A method has been developed for isolation of cells from the efferent ductules of the rat epididymis to obtain a population of epithelial cells. The technique depends on a transfer of segments of the ductules into a new isolation medium and gradual purification from fat cells, fibroblasts, collagen fibres and smooth mu...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:We have investigated immunohistochemically the effect of dl-alpha-tocopherol (vitamin E) on thyroid gland with 6-n-propyl-2-thiouracil (PTU)-induced hypothyroidism in rats. The animals were divided into four groups. Rats in group I were designated as control, rats in group II were treated with injections of PTU (10 mg...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Diplotene oocyte nucleus of the scorpionfly Panorpa communis is transcriptionally silent and contains numerous nuclear bodies including interchromatin granule clusters (IGCs). The latter consist of the granules of 30-50 nm in diameter and contain IGC marker protein SC35 as well as RNA polymerase II. In this study, we ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0006-2
更新日期：2009-01-01 00:00:00
abstract：:The effect of estradiol and progesterone on the noradrenaline (NA) content in nerve fibres of the oviduct, uterus and vagina was investigated in 37 ovariectomized sows, divided into 4 groups. A recovery period was 4 weeks. Starting from the 9th day before killing, the animals were injected 10 times (1 injection/day; t...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Culture of preimplantation embryos is complex and requires strictly defined culture media to sustain their viability and quality. In the current study, an effort was made to evaluate comprehensively the quality of mice embryos, grown in media enriched with IGF I, IGF II, EGF and TNFalpha. For that purpose, critically ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The present work deals with the histochemical, histoenzymological and micro-analytical study of the stomach of Littorina Littorea (L.). The results suggest the existence of intracellular digestion within the stomach cells. Thus, the occurrence of lysosomes in the mid-cytoplasm of stomach cells has been revealed by dem...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
