Localization of a proteasomal antigen in human spermatozoa: immunohistochemical electron microscopic study.

Abstract:

:The ultrastructural localization of a proteasomal antigen in human spermatozoa was studied by means of immunolabeling with the MPC21 monoclonal antibody and secondary gold labeled antibody with 1.4 nm gold particles in combination with silver enhancement reaction using pre-embedding technique. The labeling was found in the acrosomal and postacrosomal regions, in the connecting-piece (neck) and, in some cases, in the middle-piece and also in the residual bodies. There was no significant reaction in condensed chromatin. In some abnormal forms of spermatozoa, in which the chromatin was not well condensed, the labeling in nuclei was present. The nuclear vacuoles with looser chromatin were usually strongly labeled. The nuclei of cells representing different stages of spermatogenesis, that were present in semen samples, were also labeled.

authors

Biały LP,Ziemba HT,Marianowski P,Fracki S,Bury M,Wójcik C

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

129-30

issue

2

eissn

0239-8508

issn

1897-5631

journal_volume

39

pub_type

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