Abstract:
:In this study, the histological, immunohistochemical, morphometric, and biochemical changes to pancreatic beta-cells in STZ-induced diabetes were evaluated in rats treated with different doses of caffeine. Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control group, the diabetic untreated group, and three diabetic groups treated with different doses of caffeine (10, 50, and 100 mg/kg/day). Blood glucose and serum insulin levels were measured. The pancreata were collected and processed into paraffin sections. They were stained using hematoxylin and eosin (H&E) and Masson trichrome stains. The insulin expression in beta-cells was assessed using immunohistochemistry. Morphometrically, the percentage area of anti-insulin antibody reaction, the percentage of beta-cells per total islet cell number, and the average area of the islets were determined. STZ-induced degenerative changes in beta-cells led to decreases in the number of functioning beta-cells and insulin immunoreactivity and to increases in the number of collagen fibers in the islets. In STZ-treated rats, caffeine significantly decreased blood glucose concentration while increasing blood insulin levels at the highest applied dose. It also induced a significant increase in the number of immunoreactive beta-cells. In conclusion, caffeine may have a protective role in the biochemical and microscopic changes in pancreatic beta-cells in diabetes induced in rats through STZ administration.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Abunasef SK,Amin HA,Abdel-Hamid GAdoi
10.5603/FHC.2014.0005subject
Has Abstractpub_date
2014-01-01 00:00:00pages
42-50issue
1eissn
0239-8508issn
1897-5631pii
VM/OJS/J/35016journal_volume
52pub_type
杂志文章abstract::Two hypotheses explain the role of adult progenitor cells in myocardial regeneration. Stem cell plasticity which involves mobilization of stem cells from the bone marrow and other niches, homing to the area of tissue injury and transdifferentiation into functional cardiomyocytes. Alternative hypothesis is based on the...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract::The aim of this study was to estimate distant results after discontinuation of long term lamivudine treatment in children with chronic hepatitis B. Furthermore, the emergence of HBV polymerase gene variants in YMDD motif during therapy was examined. Additionally, the most commonly occurring type of mutation in the pol...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-08-0107-3
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abstract::On the basis of radioactivity of interphase cells in the antheridial filaments of Chara vulgaris after a 20 min. incubation with 3H-uridine and after a 40 min. postincubation the activity of synthesis and transport of newly synthesized RNA has been studied in different-sized nucleoli. Investigations of their ultrastru...
journal_title:Folia histochemica et cytobiologica
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更新日期:1985-01-01 00:00:00
abstract::Nitric oxide (NO) is produced by NO synthase (NOS) isoforms: neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS). It is believed that, while nNOS and eNOS are effective in regulation of normal physiological processes, iNOS is expressed at an increasing rate especially in inflammatory process. The aim ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-008-0032-5
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abstract::In cervical cancer, glycosylation has been suggested as being involved in both its carcinogenesis and invasive capacity. In this work, we analyzed mucin type O-glycosylation in biopsies of invasive cervical cancer in FIGO stage II B through histochemistry using lectins specific for O-glycosidically linked glycans. Our...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/19748
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abstract:INTRODUCTION:Sucla2, a β subunit of succinyl coenzyme A synthase, is located in the mitochondrial matrix. Sucla2 catalyzes the reversible synthesis of succinate and adenosine triphosphate (ATP) in the tricarboxylic acid cycle. Sucla2 expression was found to be correlated with the capacitation of boar spermatozoa. We ha...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2016.0020
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abstract::Platinum-based chemotherapy is the main type of I-line treatment of advanced and non-operative NSCLC patients without EGFR gene mutation. The excision repair cross-complementation group 1 (ERCC1) is an enzyme that executes the incision of the damaged DNA strand and removes platinum-induced DNA adducts. We investigated...
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abstract::Immunohistochemical localization of androgen receptor (AR) was investigated in the rat oocytes during their development in the primary, secondary and antral follicles. The group of experimental Wistar rats included prepubertal female rats, killed at 30 days of age, and mature female rats killed at estrus or metestrus....
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
abstract::Tuberculosis (TB) still remains the leading cause of mortality due to bacterial pathogen. The only currently available vaccine against TB, Bacille Calmette-Guérin (BCG) is at best credited with a 50% overall protective efficacy. Skin testing with purified protein derivative (PPD) from Mycobacterium tuberculosis is the...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-008-0044-1
更新日期:2008-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2016.0023
更新日期:2016-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-010-0037-8
更新日期:2010-09-30 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::The fossil dinosaur bone material, 80 million years old was studied. Samples for analysis were prepared with specially elaborated methods. Images obtained in the light, transmission electron, and scanning electron microscopes revealed the spatial distribution of the osteocytes lying near and far from the vascular cana...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.5603/17843
更新日期:2012-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
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doi:10.2478/18710
更新日期:2012-04-24 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2017.0011
更新日期:2017-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2018.0011
更新日期:2018-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.5603/fhc.2011.0069
更新日期:2011-01-01 00:00:00
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journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
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