Abstract：
:Lipotubuloids, structures containing lipid bodies and microtubules, are described in ovary epidermal cells of Ornithogalum umbellatum. Microtubules of lipotubuloids can be fixed in electron microscope fixative containing only buffered OsO(4) or in glutaraldehyde with OsO(4) post-fixation, or in a mixture of OsO(4) and glutaraldehyde. None of these substances fixes cortical microtubules of ovary epidermis of this plant which is characterized by dynamic longitudinal growth. However, cortical microtubules can be fixed with cold methanol according immunocytological methods with the use of β-tubulin antibodies and fluorescein. The existence of cortical microtubules has also been evidenced by EM observations solely after the use of taxol, microtubule stabilizer, and fixation in a glutaraldehyde/OsO(4) mixture. These microtubules mostly lie transversely, sometimes obliquely, and rarely parallel to the cell axis. Staining, using Ruthenium Red and silver hexamine, has revealed that lipotubuloid microtubules surface is covered with polysaccharides. The presumption has been made that the presence of a polysaccharide layer enhances the stability of lipotubuloid microtubules.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Kwiatkowska M,Stępiński D,Polit JT,Popłońska K,Wojtczak Adoi
10.5603/fhc.2011.0040subject
Has Abstractpub_date
2011-01-01 00:00:00pages
285-90issue
2eissn
0239-8508issn
1897-5631journal_volume
49pub_type
杂志文章abstract：:The biology of breast cancer is closely releted to sex steroid hormones. Estrogen receptor beta is overexpressed in around 70% breast cancer cases, referrd to as "ER positive". Estrogens bind to estrogen receptor and stimulate the transcription of genes involved in control of cell proliferation. Moreover, estrogens ma...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0047-6
更新日期：2009-01-01 00:00:00
abstract：:A literature survey was performed of human cathepsin D gene, cathepsin D biosynthesis, posttranslatory modifications, transport within the cell, substrate specificity and catalytic effect. Methods used to determine the activity and level of this proteinase as well as its role in the biochemistry and pathobiochemistry ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：10.2478/v10042-008-0003-x
更新日期：2008-01-01 00:00:00
abstract：:The presence and pattern of coexistence of catecholamine-synthesizing enzymes and some neuropeptides in nerve fibres supplying thoraco-cranial arteries of the sexually immature gilts were investigated in whole mount preparations. The studied substances included: tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (D ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:STAT3 upregulates expression of HIF-1 induced EPO. Receptor EPOR was reported to activate STAT3. Our study was aimed at demonstration of tissue immunoreactivities of those proteins and determination of their relationships in reference to clinicopathological variables of breast cancers. We detected STAT3, HIF-1alpha, E...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The study was undertaken to determine the immunoexpression of protein products of nm23 genes which are thought to be potential metastasis suppressor genes, in esophageal squamous cell carcinoma, and to analyze their relationship to selected clinicopathological features (age, sex, tumour size, depth of invasion, presen...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Human chromosome carry different fragile sites, but the most interesting one is the fragile X chromosome, especially in relation to mental retardation. In this report we present micronucleus formation in 5 individuals with the fragile X syndrome and 11 control subjects whose lymphocytes were cultured under 1) hydroxyu...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:The aim of this study was to visualize the expression of androgen receptor, cytochrome P450 aromatase and FSH receptor mRNAs in various structures of porcine ovary. Porcine ovaries were frozen in liquid nitrogen, and 8 microm sections were prepared for in situ hybridization. In the small, medium and large antral folli...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Recently, the potential risk of malignant cancer development in psoriatic patients has been highlighted. However, the relationship between the therapeutic schemes in psoriasis and possible neoplastic transformation has not been so far clearly explained. Phototherapy is considered a very effective therapeutic method in...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：10.5603/17843
更新日期：2012-01-01 00:00:00
abstract：:Primary lung cancer remains the leading cause of cancer death worldwide. Promoter hypermethylation is a major inactivation mechanism of tumor-related genes, and increasingly appears to play an important role in carcinogenesis. In the present study, we used quantitative methylation-specific PCR (Q-MSP) assays to analyz...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0019
更新日期：2011-01-01 00:00:00
abstract：:We report here about the purification of prion protein 27-30 (PrP 27-30) and scrapie-associated fibrils (SAF) from hamsters infected with the 263K strain of scrapie. Ultrastructural analysis of fractions from scrapie-infected brains revealed numerous fibrils measuring approximately 20 nm in diameter and 100-200 nm in ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Culture of preimplantation embryos is complex and requires strictly defined culture media to sustain their viability and quality. In the current study, an effort was made to evaluate comprehensively the quality of mice embryos, grown in media enriched with IGF I, IGF II, EGF and TNFalpha. For that purpose, critically ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Evidence has accumulated that cancer develops from a population of quiescent tissue committed/pluripotent stem cells (TCSC/PSC) or cells developmentally closely related to them that are distributed in various organs. To support this notion, stem cells (SC) are long lived cells and thus may become the subject of accumu...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：2005-01-01 00:00:00
abstract：:The Ubiquitin-Proteasomes System (UPS) and autophagy, two main intracellular protein degradation pathways within the eukaryotic cells which were originally regarded as rather independent, seem to be very closely related. Proteasome inhibitors, including the multipathway inhibitor bortezomib, are drawing increased atte...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：10.5603/FHC.2013.0036
更新日期：2013-01-01 00:00:00
abstract：:Development of thymic medulla was examined on consecutive gestational days (GD) in Wistar rats. Medullary thymic epithelial cells (TEC) were identified by immunocytochemical localisation of neuron-specific enolase (NSE). Organisation of thymic medullary architecture was determined by interaction of thymocytes with NSE...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Therapeutic outcomes of definitively treated non-small-cell lung cancer (NSCLC) are unacceptably poor. It has been proposed that the manipulation of dendritic cells (DCs) as a "natural" vaccine adjuvant may prove to be a particularly effective way to stimulate antitumor immunity. Presently, there is no standardized me...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0078-4
更新日期：2008-01-01 00:00:00
abstract：:The advent of DNA and RNA labelling techniques which allow non-autoradiographic detection of specific nucleic acid sequences has opened new possibilities for rapid diagnosis at the nucleic acid level. There are already practical applications. However, in order to fully compete with radioactive procedures an increase i...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:The effect of limited amino acid nutrition upon the growth of B16 melanoma cells was investigated. It was observed that the cells in Basal Medium Eagle do not proliferate and are inhibited predominantly in the G1 phase of cell cycle. The cells which were inhibited in growth for 7 or 12 days in Basal Medium Eagle were ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：INTRODUCTION:Previous studies analyzing ghrelin and obestatin expression in thyroid gland tissue are not unanimous and are mostly related to ghrelin. The role of ghrelin and obestatin in the thyroid gland appears very interesting due to their probable involvement in cell proliferation. Furthermore, since the thyroid gl...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2015.0004
更新日期：2015-01-01 00:00:00
abstract：:The intra-S-phase checkpoint response to hydroxyurea (HU)-mediated arrest of DNA replication was analysed in root meristems of two legumes, Pisum sativum and Vicia faba. The obtained results suggest that a molecular signal which invokes mechanisms allowing the cells to override the S-M dependency control system may be...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The influence of bilateral destruction of dopaminergic endings in the anterior and in the posterior part of nucleus accumbens (NAS) and in the nucleus septi lateralis (NSL), by 6-hydroxydopamine (6-OHDA) infusions, on the facilitatory effect of cholecystokinin-unsulfated octapeptide (CCK-8US) and caerulein (CER) on me...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Early recognition of prostate cancer (PC) based on biological markers could be helpful in identification of differences in benign and malignant lesions and facilitate further precise indication for more aggressive treatment. Therefore, the aim of our study was to assess expression of hTERT (human telomerase reverse tr...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.2013.0010
更新日期：2013-01-01 00:00:00
abstract：:The induction of exercise-induced apoptosis in not actively involved in exercise organs, such as kidney could be a result of oxidative stress. Metallothionein (MT) exerts a protective effect in the cell against oxidative stress and apoptosis. We have previously demonstrated an increased incidence of apoptosis in dista...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Uterine leiomyoma is the most common tumour in women with a reported incidence of 25-30%. The tumors are benign, composed of smooth muscle cells with variable amount of collagen - rich fibrous tissue. It is well established that accumulation of extracellular matrix in leiomyoma is key feature of tissue fibrosis. Howev...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The level of transglutaminase activity has been investigated by the fluorescence method in peritoneal macrophages of control and transplantable melanomas bearing hamsters. If the transglutaminase activity is concerned the macrophages in no one of the groups examined were a homogenous population. Differences in macroph...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:The expression of proliferating cell nuclear antigen (PCNA) correlates to cell proliferation and for this reason it is commonly considered as one of proliferation markers. Since proliferation rate is an important factor determining the tumor aggressiveness, the evaluation of PCNA index (the percentage of PCNA-immunopo...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Nuclear bodies (NBs) are intranuclear structures described in normal and pathologically altered cells of humans and animals. The NBs are 0.3-1.5 microns round structures. Their function is unknown. In the present paper, we describe NBs in the nuclei of the conjunctiva epithelial cells in one patient with cicatricial p...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Recently identified biological risk factors in B-cell chronic lymphocytic leukemia (B-CLL) include ZAP-70 and CD38 expression. The present study was conducted to clarify whether a combined analysis could improve predictive impact of these two parameters. We examined the expression of ZAP-70 and CD38 by flow cytometry ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0022-7
更新日期：2008-01-01 00:00:00
abstract：:The present study deals with immunohistochemical localization of S-100 protein in mouse, bank vole and pine vole testis and epididymis. S-100 protein immunoreactivity was observed in the endothelia of capillaries and lymphatic sinusoids of pine vole testis. A reaction to S-100 protein of the same intensity as that not...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Pancreatic acinar cell metaplasia of the gastric mucosa is a newly recognized entity. Its physiological relevance and association with other gastric pathological conditions remains to be elucidated. We studied morphology of pancreatic metaplasia of the gastric mucosa in 15 cases. Two morphological forms of pancreatic ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The supramolecular dye Congo red was used to check whether monocyte activation may be mediated by a torsion-dependent mechanism preventing transduction of weak random signals in cell contacts in a way corresponding to the discrimination mechanism found in complement fixation by immune complexes. Tight cell-cell contac...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
