Abstract:
:The supramolecular dye Congo red was used to check whether monocyte activation may be mediated by a torsion-dependent mechanism preventing transduction of weak random signals in cell contacts in a way corresponding to the discrimination mechanism found in complement fixation by immune complexes. Tight cell-cell contacts generating torsional effects may be expected to produce alteration of receptor structure, making them accessible for binding of supramolecular dyes. In this study, Congo red was used to observe the binding accessibility of (1) monocytes (human) induced by contact with cancer cells (HCV29T, human), (2) monocytes (mouse) stimulated by interaction with heat-aggregated IgG and (3) monocytes (mouse) activated by rosetting in the presence of an SRBC-anti-SRBC system. Microscopic studies confirmed the activation of monocytes manifested by their clustering and Congo red binding, but only tightly clustered cells appeared to attach the dye on the surface. Usually not the whole cell surface is found to be engaged in dye complexation. Staining occurs predominantly on the interfaces of reacting cells, making probable the suggestion that cell adhesion receptors are involved in dye binding. The cells in the central areas of tight clusters undergo accelerated death. In the presence of Congo red they are easily recognized as intensely fluorescent. The characteristic localization of dead cells in the central area of clusters indicates that death is not random but results from cell activation. The role of Congo red in this process remains to be clarified. The staining characteristics of monocytes after application of Congo red probably discloses the initial step in signal transduction generated by torsional movements in receptor proteins.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Zemanek G,Rybarska J,Stopa B,Piekarska B,Spólnik P,Konieczny L,Roterman I,Bugajski Asubject
Has Abstractpub_date
2003-01-01 00:00:00pages
113-24issue
3eissn
0239-8508issn
1897-5631journal_volume
41pub_type
杂志文章abstract:INTRODUCTION:Testicular function in the sand rodent Psammomys obesus is subjected to seasonal alternations with a trigger of spermatogenesis in winter and a total quiescence which extends from late spring to summer. The aim of this study was to investigate the distribution of b-endorphin in the testis at the period of ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2016.0013
更新日期:2016-01-01 00:00:00
abstract::In cases of decomposed bodies Y chromosomal STR markers may be useful in identification of a male relative. The authors assessed typeability PowerPlex Y (Promega) loci in tissue material stored in water and soil environment. Tissue material was collected during autopsies of five persons aged 20-30 years with time of d...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::T cell memory in comparison with B cell memory is not well understood. This review focuses on CD8+ and CD4+ memory T cells. In this article we try to define memory cells and also present models of memory T cells formation. We would also like to delineate their differentiation into distinct subsets. Long-lived memory T...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.2478/v10042-008-0077-5
更新日期:2008-01-01 00:00:00
abstract::The use of cancer vaccines based on dendritic cells (DC) presenting tumor antigens can be a promising tool in the treatment of leukemia. The functional characteristics of leukemia derived DC is still to be elucidated. CD40 promotes survival, proliferation and differentiation of normal B cells. CD40 triggering was used...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Sucla2, a β subunit of succinyl coenzyme A synthase, is located in the mitochondrial matrix. Sucla2 catalyzes the reversible synthesis of succinate and adenosine triphosphate (ATP) in the tricarboxylic acid cycle. Sucla2 expression was found to be correlated with the capacitation of boar spermatozoa. We ha...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2016.0020
更新日期:2016-01-01 00:00:00
abstract::A simple chemically defined medium is described which in primary cultures promotes differentiation of cells form embryonic chicken breast muscle to myotubes and cross-striated myofibres. It is shown that 5 minute pulse treatment of the myogenic cells with the insulin containing solution is sufficient to switch on, in ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract:INTRODUCTION:Osteoarthritis (OA) is a severe joint degeneration disease in elderly people described by the advanced degradation of articular cartilage, which ultimately leads to chronic pain. Trans-cinnamaldehyde (TCA) exerted its anti-inflammatory function in numerous disease syndromes; however, its role in the pathog...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2020.0025
更新日期:2020-01-01 00:00:00
abstract::Delayed type hypersensitivity reaction (DTH) consists of a sequential cascade of steps depending on different types of T cells, as well as mast cells, endothelial cells and macrophages. Recently it has been shown that CD4+ TH1 lymphocytes ("inflammatory type") play a central role in DTH reaction. Activated TH1 cells p...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:
更新日期:1990-01-01 00:00:00
abstract::Stem cells for skeletal muscle originate from dermomyotome of the embryo. The early marker of these cells is expression of both transcription factors Pax3 and Pax7 (Pax3+/Pax7+ cells). The skeletal muscles in the adult organism have a remarkable ability to regenerate. Skeletal muscle damage induces degenerative phase,...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
abstract::Development of thymic medulla was examined on consecutive gestational days (GD) in Wistar rats. Medullary thymic epithelial cells (TEC) were identified by immunocytochemical localisation of neuron-specific enolase (NSE). Organisation of thymic medullary architecture was determined by interaction of thymocytes with NSE...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Embryo implantation consists of a series of events promoting the invasion of the endometrium and then the uterine arterial system by the extra-embryonic trophoblast. In order for this semi-heterologous implantation to succeed, the endometrium has to first undergo a number of structural and biochemical changes (decidua...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0058-3
更新日期:2009-01-01 00:00:00
abstract::The development of central serotoninergic neurons has been investigated with immunohistochemical techniques using the indirect peroxidase-antiperoxidase (PAP) method in 16-and 19-day-old rat embryos, in 1, 10 and 26 days old young and in adult animals. Immunoreactive neurons were present on embryonic day 16 in the sub...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::The biology of breast cancer is closely releted to sex steroid hormones. Estrogen receptor beta is overexpressed in around 70% breast cancer cases, referrd to as "ER positive". Estrogens bind to estrogen receptor and stimulate the transcription of genes involved in control of cell proliferation. Moreover, estrogens ma...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0047-6
更新日期:2009-01-01 00:00:00
abstract::Primary lung cancer remains the leading cause of cancer death worldwide. Promoter hypermethylation is a major inactivation mechanism of tumor-related genes, and increasingly appears to play an important role in carcinogenesis. In the present study, we used quantitative methylation-specific PCR (Q-MSP) assays to analyz...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/fhc.2011.0019
更新日期:2011-01-01 00:00:00
abstract::Dendritic cells (DCs) are regarded as the most potent antigen presenting cells that are well suited to activate T cells toward various antigens, such as tumor-associated antigens, due to their costimulatory activity. There is evidence that DCs are of diverse origin, with at least two types of myeloid and lymphoid prec...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::A literature survey was performed of the methods of quantitative assessment of the activity and concentration of cathepsin D and its inhibitors. Usefulness of non-modified and modified proteins and synthetic peptides as measurement substrates was evaluated. The survey includes also chemical and immunochemical methods ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.2478/v10042-009-0073-4
更新日期:2009-01-01 00:00:00
abstract::To date four types of glial cells have been identified in central nervous system: astrocytes, oligodendrocytes, microglia, ependymocytes. The latest results indicate the existence of the fifth glial type-synantocytes from the Greek word synanto that is for contact. Synantocyte processes reach neurons, astrocytes, olig...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.2478/v10042-010-0033-z
更新日期:2010-01-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is an endocrinological and metabolic disorder which may concern about 3-8% of women. Some PCOS women have the increased leptin concentration in blood serum. Leptin concentration is higher in patients with high body mass index (BMI) and impaired tissue sensitivity to insulin. The aim of...
journal_title:Folia histochemica et cytobiologica
pub_type: 临床试验,杂志文章
doi:10.2478/v10042-009-0032-0
更新日期:2009-01-01 00:00:00
abstract::The influence of bilateral destruction of dopaminergic endings in the anterior and in the posterior part of nucleus accumbens (NAS) and in the nucleus septi lateralis (NSL), by 6-hydroxydopamine (6-OHDA) infusions, on the facilitatory effect of cholecystokinin-unsulfated octapeptide (CCK-8US) and caerulein (CER) on me...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::The relationship between alcohol consumption and glycoconjugate metabolism is complex and multidimensional. This review summarizes the advances in basic and clinical research on the molecular and cellular events involved in the metabolic effects of alcohol on glycoconjugates (glycoproteins, glycolipids, and proteoglyc...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章,评审
doi:10.2478/18690
更新日期:2012-04-24 00:00:00
abstract::The increased ambient temperature affects the function of hypothalamic-pituitary-adrenal (HPA) axis. Since the correlation among vasopressin (VP), adrenocorticotropic hormone (ACTH) and corticosterone (CORT) responses to various stressors have been long recognized, the aim of this study was to reveal the aforementione...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-010-0071-6
更新日期:2010-12-01 00:00:00
abstract::The effect of limited amino acid nutrition upon the growth of B16 melanoma cells was investigated. It was observed that the cells in Basal Medium Eagle do not proliferate and are inhibited predominantly in the G1 phase of cell cycle. The cells which were inhibited in growth for 7 or 12 days in Basal Medium Eagle were ...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:INTRODUCTION:Overexpression of growth hormone (GH) leads to increase in insulin-like growth factor-1 (IGF-1) plasma level, stimulation of growth and increase in body size, organomegaly and reduced body fat. The action of GH affects all the organs and transgenic mice that overexpress bovine GH (bGH mice) serve as conven...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/fhc.a2015.0024
更新日期:2015-01-01 00:00:00
abstract::The death and phagocytosis of the stomach mucoid epithelium cells in 2-8 day old mice were investigated in relation to the state of cell differentiation and the cell age. The autoradiographic determination of 3H-thymidine and cytochemical demonstration of neutral mucosubstances in secretory granules (TA-UA and PA-TCH-...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::A comparison of the number of mRNA molecules of histone H3 in 1 microg total RNA extracted from colon sections sampled during colonoscopy was used to evaluate cellular proliferation activity in the rectum and sigmoid, in normal tissue and in colitis ulcerosa. Samples with similar intensity of the disease were selected...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Preterm labour and prematurity are still a main cause of perinatal morbidity nowadays. The aim of our study was to assess the role of MMP-8 as a predictive marker of preterm delivery. Four groups of patients were involved to the study: I - pregnant women at 24-34 weeks of gestation with any symptoms of threatened pret...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-010-0037-8
更新日期:2010-09-30 00:00:00
abstract::Spermatogenesis involves the realization of a particular genetic program which requires a specific environment ("niche"). Multiplication, differentiation and apoptosis of male germ cells are finely regulated by pituitary hormones (mainly LH and FSH), and by a complex network of factors originating from both the somati...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/v10042-009-0061-8
更新日期:2009-01-01 00:00:00
abstract::Tyrosine phosphorylation of numerous proteins is one of the earliest events detectable during Fcgamma receptor-mediated phagocytosis. We demonstrate that IgG-coated particles associated with the surface of macrophages are enriched with numerous tyrosine-phosphorylated proteins. During particle internalization the prot...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract:INTRODUCTION:Aging causes morphological and functional changes in the thyroid gland. Free radicals play a key role in the pathology of normal aging. Vimentin and cytokeratin are cytoskeletal intermediate filaments that are often used as indirect indices of tissue injury. The aim of the study was to clarify the age-rela...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.5603/FHC.a2018.0015
更新日期:2018-01-01 00:00:00
abstract::Platinum-based chemotherapy is the main type of I-line treatment of advanced and non-operative NSCLC patients without EGFR gene mutation. The excision repair cross-complementation group 1 (ERCC1) is an enzyme that executes the incision of the damaged DNA strand and removes platinum-induced DNA adducts. We investigated...
journal_title:Folia histochemica et cytobiologica
pub_type: 杂志文章
doi:10.2478/18700
更新日期:2012-04-24 00:00:00