Abstract：
:During alpaca testis development and spermatogenesis, nerve growth factor (NGF) may play an important role. The main aim of this study was to determine the expression and localization of NGF in the alpaca testis, and to discuss the important role of NGF in alpaca reproductive characteristics. Immunohistochemical staining technique and real-time PCR were used. The expression of NGF in the same cells one-month old (newborn) alpacas 12-month, and 24-month old alpacas showed significant differences (p < 0.05); 12- and 24-month old alpacas showed no significant differences (p > 0.05); NGF at different cell stages showed no significant differences (p > 0.05). It suggests that NGF may be involved in the regulation of spermatogenesis, which provides direct evidence for NGF action in the alpaca testis during postnatal development and spermatogenesis.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Wang H,Dong Y,Chen W,Hei J,Dong Cdoi
10.5603/fhc.2011.0009subject
Has Abstractpub_date
2011-01-01 00:00:00pages
55-61issue
1eissn
0239-8508issn
1897-5631journal_volume
49pub_type
杂志文章abstract：:A literature survey was performed of human cathepsin D gene, cathepsin D biosynthesis, posttranslatory modifications, transport within the cell, substrate specificity and catalytic effect. Methods used to determine the activity and level of this proteinase as well as its role in the biochemistry and pathobiochemistry ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：10.2478/v10042-008-0003-x
更新日期：2008-01-01 00:00:00
abstract：:The purpose of the present study was to evaluate morphologically the response of feline teeth pulp to direct pulp capping with two different self-etch adhesive systems. Twenty-four cavities in feline teeth were mechanically exposed and assigned to one of two experimental groups: AdheSE + Tetric Ceram (the ASE group), ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/20325
更新日期：2012-01-01 00:00:00
abstract：:Using morphological criteria we describe the effect of maternal deprivation on the gonadotrophin-releasing hormone (GnRH) and GnRH-associated peptide (GAP) of the GnRH prohormone (proGnRH) in the preoptic area (POA)-hypothalamus during the weaning period. The immunohistochemical GnRH- and GAP-neuroanatomy was investig...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0108-2
更新日期：2010-01-01 00:00:00
abstract：:The aim of this study was to visualize the expression of androgen receptor, cytochrome P450 aromatase and FSH receptor mRNAs in various structures of porcine ovary. Porcine ovaries were frozen in liquid nitrogen, and 8 microm sections were prepared for in situ hybridization. In the small, medium and large antral folli...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Diplotene oocyte nucleus of the scorpionfly Panorpa communis is transcriptionally silent and contains numerous nuclear bodies including interchromatin granule clusters (IGCs). The latter consist of the granules of 30-50 nm in diameter and contain IGC marker protein SC35 as well as RNA polymerase II. In this study, we ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0006-2
更新日期：2009-01-01 00:00:00
abstract：INTRODUCTION:Sucla2, a β subunit of succinyl coenzyme A synthase, is located in the mitochondrial matrix. Sucla2 catalyzes the reversible synthesis of succinate and adenosine triphosphate (ATP) in the tricarboxylic acid cycle. Sucla2 expression was found to be correlated with the capacitation of boar spermatozoa. We ha...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2016.0020
更新日期：2016-01-01 00:00:00
abstract：:A literature survey was performed of the methods of quantitative assessment of the activity and concentration of cathepsin D and its inhibitors. Usefulness of non-modified and modified proteins and synthetic peptides as measurement substrates was evaluated. The survey includes also chemical and immunochemical methods ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：10.2478/v10042-009-0073-4
更新日期：2009-01-01 00:00:00
abstract：:Parathyroid hormone-related peptide (PTHrP) is the cause of humoral hipercalcaemia of malignancy syndrome (HHM). It is known that the peptide as well as its receptors are widely distributed in many normal organs and tissues, where it influences an array of diverse functions which are realized through paracrine or auto...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:The biology of breast cancer is closely releted to sex steroid hormones. Estrogen receptor beta is overexpressed in around 70% breast cancer cases, referrd to as "ER positive". Estrogens bind to estrogen receptor and stimulate the transcription of genes involved in control of cell proliferation. Moreover, estrogens ma...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0047-6
更新日期：2009-01-01 00:00:00
abstract：:Light microscopic histochemical studies to visualize semicarbazide-sensitive and H2O2-generating amine oxidase (SSAOX, benzylamine oxidase, BAOX; EC 1.4.3.6?) are usually performed with the coupled peroxidatic oxidation technique of Ryder et al. [25]. For methodological reasons this procedure has its limitations and w...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Histochemical and immunohistochemical methods should often but not always complement standardized histopathologic procedures. Here, we illustrate use of these ancillary techniques in a report of scrotal leiomyosarcoma. 62-year-old male patient presented with a palpable, subcutaneous 2,5 cm wide tumor arising from dart...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.2013.0046
更新日期：2013-01-01 00:00:00
abstract：:Very suggestive evidence for possible role of beta-endorphin (BE) in immune activity has been continuously accumulated in the literature in spite of contradictory results about the involvement of the opiate system in immunological response. The reversed passive Arthus reaction (RPAR) was performed in the dorsal area o...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Early recognition of prostate cancer (PC) based on biological markers could be helpful in identification of differences in benign and malignant lesions and facilitate further precise indication for more aggressive treatment. Therefore, the aim of our study was to assess expression of hTERT (human telomerase reverse tr...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.2013.0010
更新日期：2013-01-01 00:00:00
abstract：:The advent of DNA and RNA labelling techniques which allow non-autoradiographic detection of specific nucleic acid sequences has opened new possibilities for rapid diagnosis at the nucleic acid level. There are already practical applications. However, in order to fully compete with radioactive procedures an increase i...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Different cytokine profiles allow to divide the CD4+ lymphocytes into Th1, Th2 and Th0 subtypes. It has been observed that the Th2 cells are more efficient supporters for HIV-1 replication than the Th1 cells. The Th1 and the Th2 cells were isolated from peripheral blood lymphocytes of HIV-1 seronegative individuals an...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:The ability to preselect the sex of human offspring would have a significant impact on the preventing of sex chromosome-linked diseases and on human reproduction. Numerous methods have been reviewed including: Sephadex gel filtration, centrifugation in density gradients, separation in albumin gradient, laminar flow, c...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:Coccoids (Coccinea, Coccoidea, Coccomorpha, scale insects, scales) are a highly diverse group of ectoparasitic insects. They comprise 2 subgroups: primitive archaeococcoids (= Orthezioidea sensu Koteja) and advanced neococcoids (= Coccoidea sensu Koteja). The ovaries of coccoids consist of numerous short telotrophic-m...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：1998-01-01 00:00:00
abstract：:STAT3 upregulates expression of HIF-1 induced EPO. Receptor EPOR was reported to activate STAT3. Our study was aimed at demonstration of tissue immunoreactivities of those proteins and determination of their relationships in reference to clinicopathological variables of breast cancers. We detected STAT3, HIF-1alpha, E...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The effect of cadmium (Cd) on the thyroid and parathyroid glands was examined following a 12-week exposure of rats to 5 or 50 mg Cd/dm3. Immunohistochemical methods were used to determine calcitonin (CT), calcitonin-gene related peptide (GGRP), parathormone (PTH), somatostatin (ST), synaptophysin (SPh) and neuron-spec...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:To date four types of glial cells have been identified in central nervous system: astrocytes, oligodendrocytes, microglia, ependymocytes. The latest results indicate the existence of the fifth glial type-synantocytes from the Greek word synanto that is for contact. Synantocyte processes reach neurons, astrocytes, olig...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：10.2478/v10042-010-0033-z
更新日期：2010-01-01 00:00:00
abstract：:At present, not much is known about progesterone receptor (PR) expression and localization in postmenopausal women ovaries. In the ovaries of reproductive age women, PR is localized in internal theca and granulosa cells, corpus luteum, ovary surface epithelium (OSE) and in stroma. PR expression depends on the serum co...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0038-z
更新日期：2008-01-01 00:00:00
abstract：:In telotrophic ovary of Creophilus maxillosus, the differentiation of the oocyte and nurse cells takes place within the linear clusters of sister oogonial cells. The amplification of rDNA occurs in the nuclei of pro-oocytes which are the most posterior cells of the clusters. During the consecutive oogonial divisions e...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The study was undertaken to determine the immunoexpression of protein products of nm23 genes which are thought to be potential metastasis suppressor genes, in esophageal squamous cell carcinoma, and to analyze their relationship to selected clinicopathological features (age, sex, tumour size, depth of invasion, presen...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Colorectal cancer (CRC) is one of the most common cancers of the gastrointestinal tract and the fourth cause of cancers death in the world. Soluble adhesion molecules (CAMs) are thought to have an important role in host defense against carcinogenesis. They are biomarkers of inflammation and indicators of the immune re...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0077-0
更新日期：2009-01-01 00:00:00
abstract：:Combined (3H) estradiol autoradiography and oxytocin immunocytochemistry were used in order to study co-localization of cytoplasmic oxytocin immunoreactivity and nuclear uptake of (3H) estradiol in the forebrain of adult ovariectomized mice. Labelling with (3H) estradiol was found in subpopulations of neurons that con...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The expression of proliferating cell nuclear antigen (PCNA) correlates to cell proliferation and for this reason it is commonly considered as one of proliferation markers. Since proliferation rate is an important factor determining the tumor aggressiveness, the evaluation of PCNA index (the percentage of PCNA-immunopo...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:The present paper is the first work to determine the effect of lipopolysaccharide (LPS) in the pilocarpine model of epilepsy on the morphology of rat hippocampal astrocytes in vivo. The study involved adult male Wistar rats, which 72 hours prior to administration of pilocarpine hydrochloride (PILO) were intraperitonea...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0030
更新日期：2011-01-01 00:00:00
abstract：:The polytrophic ovarioles of Panorpa communis are differentiated into a terminal filament, germarium and vitellarium. The germarium contains cystoblasts and germ cell clusters at early stages of their differentiation. At the base of germarium the cystocytes become morphologically diversified. One of the central cells ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Embryo implantation consists of a series of events promoting the invasion of the endometrium and then the uterine arterial system by the extra-embryonic trophoblast. In order for this semi-heterologous implantation to succeed, the endometrium has to first undergo a number of structural and biochemical changes (decidua...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0058-3
更新日期：2009-01-01 00:00:00
abstract：:The fibroblast growth factors (FGFs) are multifunctional proteins that, among other roles, regulate structural reorganization of uterine and placental vascular bed during pregnancy. Thus, we analyzed mRNA and protein expression and immunohistochemical localization of FGF-1 and FGF-2, and their receptors (FGFR-1 and FG...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-010-0073-4
更新日期：2010-12-01 00:00:00
