Abstract：
:Ceramide plays an important role in regulating cell proliferation and apoptosis. Recent studies indicate that generation of ceramide in the intestine from sphingomyelin hydrolysis may be implicated in colon cancer development. The enzymes that catalyze the further hydrolysis of ceramide in the intestine have, however, not been well investigated. Our data reveal the existence of a ceramidase (EC 3.5.1.23) in rat intestinal mucosa with an optimal pH of 7.0. One milligram of mucosal protein is able to hydrolyze 44.0+/-9.6 nmol of ceramide in 1 hr. The activity is low in the proximal duodenum and increases to a plateau in the proximal jejunum. The activity is then similar throughout the small intestine, until it declines in the distal part of ileum. Some activity is also detectable in the colon. The activity increases slightly in the presence of monomeric bile salt concentrations and sharply at the critical micellar concentration. Similar patterns were observed for both primary (taurocholate) and secondary (taurodeoxycholate) bile salts. The addition of Triton X-100 enhances the ceramidase activity at optimal bile salt concentration. The reaction is linear with time for the first 20 min and the hydrolytic rate declines slowly thereafter. Finally, the activity shows a considerable resistance against tryptic degradation, as 71% of the ceramidase activity remained when the homogenates were preincubated with high concentrations of trypsin. Intestinal mucosa also has a ceramide synthesis activity, with a distribution pattern generally paralleling ceramide hydrolysis activity. In conclusion, intestinal neutral ceramidase has a distinct distribution pattern and bile salt dependence, which enables it to collaborate with intestinal sphingomyelinase in hydrolysis of sphingomyelin.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Lundgren P,Nilsson A,Duan RDdoi
10.1023/a:1010792031910subject
Has Abstractpub_date
2001-04-01 00:00:00pages
765-72issue
4eissn
0163-2116issn
1573-2568journal_volume
46pub_type
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
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journal_title：Digestive diseases and sciences
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Digestive diseases and sciences
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更新日期：1986-06-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章,多中心研究
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journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
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