Abstract:
:The androgen-signaling pathway plays a critical role in prostate cancer development and progression. We have recently demonstrated that the Wilms' tumor suppressor gene product, WT1, binds to multiple sites in the androgen receptor (AR) promoter and transcriptionally represses the AR gene promoter in vitro. We asked whether WT1 repression of the endogenous AR gene interferes in the androgen signal transduction cascade and modifies AR target gene expression. We analyzed the effect of WT1 (-/-) overexpression on an AR target gene reporter construct that contains the luciferase gene, the ElB TATA box, and two copies of the androgen-response element (ARE), the dimeric AR binding site. Luciferase activity was determined in 293 kidney and TM4 Sertoli cells, two nontumorigenic cell lines that express both AR and WT1. Cells were cotransfected by lipofectamine in the presence or absence of the synthetic androgen R1881. Results showed that overexpression of WT1 downregulates ARE-reporter gene transcription in both cell lines tested. The inhibitory effect of WT1 on the AR target gene construct was dose-dependent and androgen-independent in 293 cells, whereas in TM4 cells it was androgen-dependent. Additionally, a zinc-finger mutant WT1 (-/-) expression construct, R394W, failed to decrease luciferase activity, suggesting that WT1 downregulates the ARE-reporter gene construct activity by directly repressing the endogenous AR gene promoter. Furthermore, we analyzed the expression of WT1 and AR mRNA in several prostate cancer cell lines in order to understand the role WT1 may play in prostate cancer development and progression. Gel analysis of cDNA amplified by RT-PCR of AR and WT1 RNA from prostate cancer and non-prostatic cell lines showed that LNCaP and MDAPCa2b, two metastatic prostate cancer cell lines which are androgen-sensitive, expressed AR but not WT1. Du145 and PC3, two cell lines from advanced metastatic prostate cancer, which are characterized as androgen-independent and -insensitive, did not express AR but expressed a high level of WT1. Two non-prostatic cell lines, T47D and 293, weakly co-expressed AR and WT1. This inverse relationship between AR and WT1 expression in prostate cancer cell lines, together with WT1 repression of the AR promoter, suggest a role for WT1 in the androgen signaling pathway and in prostate cancer development and progression.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Zaia A,Fraizer GC,Piantanelli L,Saunders GFsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
1-10issue
1Aeissn
0250-7005issn
1791-7530journal_volume
21pub_type
杂志文章abstract::We report on a case of low-grade periductal stromal tumor of the breast with synchronous bilateral breast carcinoma. A 45-year-old woman came to our Hospital because of a palpable mass of the right breast. An approximately 5-cm mass was palpated in the upper right breast. The mammographic and sonographic findings of t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract::Overexpression of p53-protein appears to be a common event in primary breast cancer. It has been proposed that the presence of elevated levels of this protein may be an independent prognostic factor and may be important for the ability of a tumor to metastasize. This study was performed to evaluate the influence of im...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract:BACKGROUND:Previous studies have shown that tumor necrosis factor-alpha (TNF-alpha) encapsulated in sterically-stabilized PEGylated STEALTH liposomes (SL) can better and more safely augment the efficacy of other treatment modalities than free TNF-alpha. The aim of this study was to examine the effects of SL-TNF-alpha i...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Fulvestrant ('Faslodex') is a new type of oestrogen receptor (ER) antagonist that down-regulates the ER and has no known agonist effects. PATIENTS AND METHODS:In this open-label, Phase II trial, 30 postmenopausal Japanese women with hormone-sensitive advanced breast cancer, who had progressed on tamoxifen/t...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:Since specific epidermal growth factor receptor (EGFR) somatic mutations have been demonstrated to influence the response to anti-EGFR therapy in non-small cell lung cancer, EGFR gene mutational analysis of different types of neoplasm is under investigation. PATIENTS AND METHODS:EGFR protein expression and ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:The recent prevalence of ultrasonography and fine-needle aspiration biopsy (FNAB) has facilitated the detection and diagnosis of papillary thyroid carcinoma. However, there are still cases that are preoperatively misdiagnosed and treated as benign nodules because ultrasonographic and FNAB findings do not pro...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract::The relationship between human papillomavirus (HPV) infection and breast cancer is controversial. In this study, paraffin-embedded tissue blocks prepared from 72 patients with benign, premalignant or malignant mammary lesions were randomly collected from the Shanghai region of China and Tokushima in Japan. DNA specime...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract:BACKGROUND:This study aimed to examine the effect of apigenin on proliferation and apoptosis in HER2-overexpressing MDA-MB-453 breast cancer cells. MATERIALS AND METHODS:The antiproliferative effects of apigenin were examined by proliferation and MTT assays. The effect of apigenin on apoptotic molecules was determined...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract::Methodological aspects of testing chemosensitivity by the 6-day subrenal capsule (SRC) assay in immunocompetent mice were investigated. Human tumor xenografts, serially transplanted in athymic nude mice, were used as source of material. All drugs were given by the intravenous route. Administration of the drugs on days...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND/AIM:Recent studies implied a significant role of hypoxia-inducible factor-1α (HIF1α) in cell transformation. This study aimed to assess the effects of HIF1α on the epithelial-to-mesenchymal transition (EMT) and tumorigenesis of lung adenocarcinoma cells. MATERIALS AND METHODS:Invasion, migration and colony ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12986
更新日期:2018-11-01 00:00:00
abstract:AIM:To improve the treatment of transmissible venereal tumors (TVTs) in dogs with intratumoral injections of interleukin-2 (IL-2). PATIENTS AND METHODS:We treated 13 dogs with 18 natural TVTs with IL-2. The tumors were treated with intratumoral application of 2×10(6) units IL-2. RESULTS:Three months after injection o...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND:The potential of MTp53 knockout by oligodesoxyribonucleotide phosphothioates (ODNs) to affect proliferation, apoptosis and chemosensitivity in undifferentiated thyroid cancer (UTC) cells with a recessive MTp53 mutation was evaluated. MATERIALS AND METHODS:Transient transfections with ODNs complementary to p...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract::The complication of metastasis from the primary tumor site to the distant organ is difficult to control. Tumor cells usually spread through the vascular system: lymphatics and/or blood; or by direct extension into adjacent sites. Transplantable prostate adenocarcinoma (PA-III) cells in L-W rats produces a tumor in the...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract::The triphenylmethane derivative, aurintricarboxylic acid (ATA), but not aurin, is a potent inhibitor of angiogenesis in the chick chorioallantoic membrane. ATA alone showed potent anti-angiogenic activity in a dose-related manner. The presence of heparin or bFGF decreased the anti-angiogenic activity of ATA. ATA also ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract:BACKGROUND:A phase I/II study of docetaxel (DOC) and gemcitabine (GEM) combination for treatment-resistant ovarian cancer (OC) was conducted. MATERIALS AND METHODS:Eligible patients exhibited recurrent OC within 12 months after initial treatment, or after more than 2 chemotherapy regimens. Planned dose levels (DL) wer...
journal_title:Anticancer research
pub_type: 杂志文章,多中心研究
doi:
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:Glutathione-S-transferases (GSTs) and N-acetyltransferases (NATs) are involved in the metabolism of a wide range of carcinogenic chemicals. Allelic polymorphism of these enzymes is associated with variations in enzyme activity, hence it may affect the concentration of activated carcinogenic chemicals in the ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND:Whether high energy shock waves (HESW), generated by a piezoelectric generator, were able to activate a sonosensitizer, 5-aminolevulinic acid (ALA) and induce inhibition of cell growth in HT-29 human colorectal cancer cells was investigated. MATERIALS AND METHODS:Cell survival and cell death pathways were i...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract::Cisplatin toxicity could be decreased by adjusting its dosage to each patient. For this purpose, a limited sampling method was established and validated based on a Bayesian approach taken using the values of assays during a 5-day continuous infusion of cisplatin. Using this method, a dosing model to achieve a target p...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract:BACKGROUND/AIM:HER2-positive breast cancers eventually relapse in about one third of patients. Is anti-HER2-directed therapy with Herceptin® (trastuzumab) effective in re-treatment? Between 2008 and 2018, 216 patients with recurrent HER2-positive breast cancer (BC) were re-treated with Herceptin (HER) during first-line...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14390
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND/AIM:To validate the melanoma nomogram and improve its function in prediction of nodal dissemination by incorporating a molecular marker in the model. Microphthalmia transcription factor (MITF) is an important regulator of melanocyte homeostasis and differentiation. We have shown that the grade of MITF expres...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11266
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:We hypothesized that ancestry-mediated methylated DNA changes may drive racial and ethnic disparity in prostate cancer (PCa). To test this hypothesis, we analyzed genetic ancestry and association with DNA methylation changes in PCa disparity. MATERIALS AND METHODS:Pyrosequencing and ancestry informative mar...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13790
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND:Although in vitro and clinical studies indicate that overexpression of P-glycoprotein (Pgp), p53, or metallothionein (MT) is involved in modulating drug resistance/sensitivity of cancer cells, the clinical relevance of the overexpression remains to be elucidated. MATERIALS AND METHODS:In this paper the expr...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND:Chemotherapeutic anticancer properties are thought to derive from apoptosis pathway activation and/or cell division arrest, but animal models have also evidenced anti-angiogenic activity in some agents. PATIENTS AND METHODS:The impact of gemcitabine, irinotecan and oxaliplatin + 5-FU upon the serum markers ...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:In order to decrease surgery-related morbidity, we evaluated the reliability of the evaluation of lymph node metastasis in patients with uterine corpus cancer by positron-emission tomography (PET) with 2-[(18)F]fluoro-2-deoxy-D-glucose (FDG) before surgical staging. MATERIALS AND METHODS:Patients with newly...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:The description of the different forms of circulating PSA has opened a new strategy in the diagnosis of prostate cancer. The aim of our study was to assess the diagnostic potential of PSA and the PSA fractions in the diagnosis of benign prostate hyperplasia (BPH) and prostate cancer. PATIENTS AND METHODS:We...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract:BACKGROUND/AIM:Although insulin-like growth factor-1 (IGF-1) is well-implicated in cancer biology, the potential roles of IGF-1 variants in different types of cancer are largely unknown. The aim of the present study was to in vitro characterize several human cancers for their IGF-1 variant expression patterns. MATERIA...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::A female patient with stage IV-M1c (distant lymph node and breast metastases), chemotherapy-refractory melanoma was treated with the cytotoxic T-lymphocyte antigen 4 (CTLA-4)-inhibitory monoclonal antibody ipilimumab. At first evaluation following induction treatment, there was marked increase in the volume of the lym...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract::Human leukocyte antigen (HLA)-E and HLA-F are classified as non-classical HLA class Ib antigens. Ectopic HLA-E and HLA-F expression was recently detected in cancer cells; however, the clinical implication of their expression remains unknown. A total of 209 patients with gastric cancer were enrolled in this study. Immu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:To distinguish cholangiocarcinoma from inflammatory disease remains difficult when stricture is present in the bile duct. Endoscopic brushing cytology is a convenient method for stricture in the bile duct, however, the diagnostic sensitivity of this method for malignancy is reported to be low (<60%). Msh hom...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-03-01 00:00:00
abstract::This review aims to provide insight into treatment of malignant pleural mesothelioma (MPM) considering effects on survival, quality of life (QoL) and costs, in order to determine the value of pemetrexed in MPM treatment. Cisplatin in combination with pemetrexed or raltitrexed increased survival in MPM, whereas vinorel...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2013-09-01 00:00:00