Abstract:
BACKGROUND:To distinguish cholangiocarcinoma from inflammatory disease remains difficult when stricture is present in the bile duct. Endoscopic brushing cytology is a convenient method for stricture in the bile duct, however, the diagnostic sensitivity of this method for malignancy is reported to be low (<60%). Msh homeobox 2 is frequently expressed in carcinoma cells of epithelial origin but not in normal tissues. AIM:To assess whether MSX2 expression level in brushing samples allows differentiation of malignant from benign bile duct stricture. PATIENTS AND METHODS:Cytological brushing specimens were obtained from strictures of the bile duct during endoscopic retrograde cholangiopancreaticography (ERCP) in 71 patients. The brushing fluid was subjected to cytological diagnosis and RNA extraction. The expression level of MSX2 was evaluated by one-step real-time RT-PCR. RESULTS:MSX2 expression levels were significantly higher in malignant than in benign bile duct stricture (p=0.004). The sensitivity and specificity for cholangiocarcinoma of cytology and MSX2 expression in strictures of the bile duct were: 55.3% and 100%, and 72.3% and 58.3%, respectively. CONCLUSION:The sensitivity of MSX2 expression level for cholangiocarcinoma was much higher than that of cytology. This suggests that the evaluation of MSX2 level in ERCP brushing samples would be a useful tool to distinguish malignant from benign bile duct stricture.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Ito H,Satoh K,Hamada S,Hirota M,Kanno A,Ishida K,Unno J,Masamune A,Katayose Y,Unno M,Shimosegawa Tsubject
Has Abstractpub_date
2011-03-01 00:00:00pages
1011-7issue
3eissn
0250-7005issn
1791-7530pii
31/3/1011journal_volume
31pub_type
杂志文章abstract:AIM:This retrospective observational study evaluated the role of hypo-fractionated stereotactic radiotherapy (SRT) in patients with oligo-progressive metastatic renal cell carcinoma (mRCC) treated with first-line oral tyrosine kinase inhibitors (TKI). Data on local control, delay of further progression, and safety are ...
journal_title:Anticancer research
pub_type: 杂志文章
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journal_title:Anticancer research
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journal_title:Anticancer research
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更新日期:2003-11-01 00:00:00
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doi:
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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更新日期:2014-07-01 00:00:00
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doi:
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doi:
更新日期:2012-09-01 00:00:00
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doi:
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