Hypoxia Stimulates the Epithelial-to-Mesenchymal Transition in Lung Cancer Cells Through Accumulation of Nuclear β-Catenin.

Abstract:

BACKGROUND/AIM:Recent studies implied a significant role of hypoxia-inducible factor-1α (HIF1α) in cell transformation. This study aimed to assess the effects of HIF1α on the epithelial-to-mesenchymal transition (EMT) and tumorigenesis of lung adenocarcinoma cells. MATERIALS AND METHODS:Invasion, migration and colony formation assays were used to evaluate cell transformation. Expression of EMT-related markers were analyzed by western blot, reverse-transcription polymerase chain reaction or zymography. A luciferase assay was carried out to access the transcriptional activity of β-catenin. RESULTS:Hypoxia enhanced migration, invasion and transformation of A549 lung adenocarcinoma cells. Hypoxic stimulation promoted the expression of EMT-related markers in lung cancer cells. The expression of HIF1α was found to be involved in hypoxia-mediated modulation of expression of snail family transcriptional repressors 1 (SNAI1) and 2 (SLUG). Hypoxia enhanced nuclear accumulation and transcriptional activity of β-catenin. CONCLUSION:β-Catenin promotes expression of EMT-related genes and eventually contributes to the metastatic process.

journal_name

Anticancer Res

journal_title

Anticancer research

authors

Liu KH,Tsai YT,Chin SY,Lee WR,Chen YC,Shen SC

doi

10.21873/anticanres.12986

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

6299-6308

issue

11

eissn

0250-7005

issn

1791-7530

pii

38/11/6299

journal_volume

38

pub_type

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