当前位置： SCI文献检索 > JOURNAL OF NEUROTRAUMA期刊下所有文献 > Injury severity and sensitivity to treatment after controlled cortical impact in rats.

Injury severity and sensitivity to treatment after controlled cortical impact in rats.

Abstract：

:We sought to determine sensitivity of the cortical impact injury model of traumatic brain injury (TBI) to severity of injury and to treatment. We examined the pattern of motor and cognitive deficits and recovery following TBI over a range of injury severities, and examined the efficacy of surface-induced moderate hypothermia at three disparate injury levels. In experiment I, Sprague-Dawley rats were injured at one of eight injury severity levels from 0 mm (sham) to 2.5 mm depth of penetration. On postinjury day 1, balance beam, rotorod performance, and posture reflexes were evaluated. Motor outcome was increasingly impaired with increasing injury levels, with the pattern of deficits showing a step-like function. Cognitive deficits, assessed using water maze on day 7, were more severe for the 2.5-mm group than for the 1.6-mm injury group, while the 1.0-mm group did not differ from the sham controls. In experiments II-IV, hypothermia, 30 degrees C for 3-h duration or normothermia was applied to three injury levels: 1.0 mm, the least cortical deformation; 2.5 mm, the most deformation; and 1.6 mm, representing a level in-between. Neurologic outcome was assessed relative to shams on postinjury days 1, 3, and 5. The 1.0-mm group exhibited small deficits that recovered completely by day 3; the 1.6-mm group recovered to the level of shams by day 5, and the 2.5-mm group did not show significant recovery during the testing period. Hypothermia effectively attenuated behavioral deficits for the 1.6-mm group, but had no effect on the other two groups. These three observations--that increasing injury severity is associated with increasing motor and cognitive deficits, that injury severity is related to recovery time, and that hypothermia treatment is selectively effective--have each been reported in the human TBI population; thus, moderate cortical impact injury in rats may be a model with clinical predictability for evaluating neuroprotective therapies.

journal_name

J Neurotrauma

journal_title

Journal of neurotrauma

authors

Markgraf CG,Clifton GL,Aguirre M,Chaney SF,Knox-Du Bois C,Kennon K,Verma N

doi

10.1089/08977150150502604

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

175-86

issue

eissn

0897-7151

issn

1557-9042

journal_volume

pub_type

杂志文章

在线工具

Injury severity and sensitivity to treatment after controlled cortical impact in rats.