Thiol antioxidant, N-acetylcysteine, activates extracellular signal-regulated kinase signaling pathway in articular chondrocytes.

abstract：:TRIM62, also named DEAR1, is a member of the TRIM/RBCC family, which includes proteins with conserved RING finger, B-box and coiled-coil domains. Several reports have identified a role for this family in cancer, retroviral infection and innate immunity. In this study, the E3 ubiquitin ligase activity and subcellular l...

journal_title：Biochemical and biophysical research communications

authors： Huang F,Xiao H,Sun BL,Yang RG

更新日期：2013-03-08 00:00:00

abstract：:A tryptic peptide of bovine beta-casein (amino acid residues 184-202) was used as a model antigen to investigate how the relative position of the (helper) T cell and B cell determinants on a protein antigen influences the antibody response. Immunization with the peptide elicited a considerably higher anti-peptide resp...

journal_title：Biochemical and biophysical research communications

authors： Hachimura S,Enomoto A,Kaminogawa S

更新日期：1990-06-15 00:00:00

abstract：:We have studied the convergence of the biosynthetic lysosomal route marked by the newly synthesized lysosomal enzyme arylsulfatase A (ASA) with the endosomal/prelysosomal compartment in ASA overexpressing baby hamster kidney (BHK) cells. A monoclonal antibody against ASA conjugated to transferrin (Tf-alpha ASA) was us...

journal_title：Biochemical and biophysical research communications

authors： Diesner F,Sommerlade HJ,Braulke T

更新日期：1993-11-30 00:00:00

abstract：:The ribonucleotide reductase from the strictly anaerobic methanogen Methanobacterium thermoautotrophicum has been partially purified by ion-exchange and gel-filtration chromatography. Its molecular weight is estimated to be 100,000 by the latter step. Unlike all previously studied ribonucleotide reductases, the enzyme...

journal_title：Biochemical and biophysical research communications

authors： Sze IS,McFarlan SC,Spormann A,Hogenkamp HP,Follmann H

更新日期：1992-04-30 00:00:00

abstract：:Reactive oxygen species (ROS) are an important factor in the development of skin lesions in diabetes. A new antioxidant, hydrogen, can selectively neutralize hydroxyl radicals (()OH) and peroxynitrite (ONOO(-)) in cell-free systems, whereas it seldom reacts with other ROS. Fibroblasts are a key component of skin. In t...

journal_title：Biochemical and biophysical research communications

authors： Yu P,Wang Z,Sun X,Chen X,Zeng S,Chen L,Li S

更新日期：2011-06-03 00:00:00

abstract：:The activity of Cav1.2 Ca(2+) channels is maintained in the presence of calmodulin and ATP, even in cell-free patches, and thus a channel ATP-binding site has been suggested. In this study, we examined whether other nucleotides, such as GTP, UTP, CTP, ADP and AMP, could be substituted for ATP in guinea-pig ventricular...

journal_title：Biochemical and biophysical research communications

authors： Liu SY,Xu JJ,Minobe E,Gao QH,Feng R,Zhao MM,Guo F,Yang L,Hao LY,Kameyama M

更新日期：2015-05-08 00:00:00

abstract：:Activation of muscarinic cholinergic receptors on 1321N1 human astrocytoma cells leads to Ca2+ mobilization as measured by quin 2 fluorescence. Acetylcholine and methacholine were full and potent agonists, while carbachol and muscarine, were fully efficacious but 6- and 10-fold less potent than acetylcholine. The carb...

journal_title：Biochemical and biophysical research communications

authors： Noronha-Blob L,Richard C,U'Prichard DC

更新日期：1987-08-31 00:00:00

abstract：:In a recent publication [Biochem. Biophys. Res. Commun. 317 (2004) 957] it was proposed that the 'effective number of codons' (Nc) in a gene should be calculated by summing the individual amino acid Nc's using rounding whenever the codon homozygosities are lower than the reciprocal value of the number of members of th...

journal_title：Biochemical and biophysical research communications

authors： Fuglsang A

更新日期：2005-02-04 00:00:00

abstract：:Lupeol, present in fruits and medicinal plants, is a biologically active compound that has been shown to have various pharmacological properties in experimental studies. In the present study, we demonstrated the modulatory effect of lupeol on 7,12-dimethylbenz[a]anthracene (DMBA)-induced alterations on cell proliferat...

journal_title：Biochemical and biophysical research communications

authors： Nigam N,Prasad S,George J,Shukla Y

更新日期：2009-04-03 00:00:00

abstract：:Mitochondrial ATP synthase is responsible for production of the majority of cellular ATP. Disorders of ATP synthase in humans can be caused by numerous mutations in both structural subunits and specific assembly factors. They are associated with variable pathogenicity and clinical phenotypes ranging from mild to the m...

journal_title：Biochemical and biophysical research communications

authors： Nuskova H,Mikesova J,Efimova I,Pecinova A,Pecina P,Drahota Z,Houstek J,Mracek T

更新日期：2020-01-22 00:00:00

abstract：:Experimental gene transfer has permitted a wide variety of studies on gene regulation and function. However, possible effects of the introduced DNA on cellular metabolism are not well understood. Here we demonstrated that introduction of DNA into a promonocytic cell line, U937, by electroporation caused extensive cell...

journal_title：Biochemical and biophysical research communications

authors： Shimokawa T,Okumura K,Ra C

更新日期：2000-04-02 00:00:00

abstract：:Phosphoinositide phospholipase C (PLC) was extracted from the synaptic membrane fraction of rat brain by 1% sodium deoxycholate. The molecular weight and sedimentation coefficient of the membrane PLC were about 160,000 and 6.7 as estimated by gel filtration and sucrose density gradient centrifugation, respectively. Th...

journal_title：Biochemical and biophysical research communications

authors： Kozawa O,Hoshijima M,Tanimoto T,Ohmori T,Takai Y

更新日期：1987-05-29 00:00:00

abstract：:Inspired by recent interest in torsion of the central pair microtubules in eukaryotic flagella, a novel thin-walled elastic beam model is suggested to study critical condition under which uniform bending of a flagellum will cause lateral/torsional buckling of the central pair. The model is directed to the central pair...

journal_title：Biochemical and biophysical research communications

authors： Li C,Ru CQ,Mioduchowski A

更新日期：2006-12-08 00:00:00

abstract：:Hypoxia inducible factor-1 (HIF-1) is a transcription factor composed of two subunits, HIF-1alpha and ARNT, which is activated under hypoxia. HIF-1alpha mRNA is expressed constitutively in a wide variety of cell types, whereas in some others HIF1A gene expression is upregulated by hypoxia. In this report, we show that...

journal_title：Biochemical and biophysical research communications

authors： Minet E,Ernest I,Michel G,Roland I,Remacle J,Raes M,Michiels C

更新日期：1999-08-02 00:00:00

abstract：:Transcription factor IIA (TFIIA) is a necessary component of many RNA polymerase II transcription complexes. Assembly of the transcription complex begins when TFIIA interacts with the promoter. We have previously purified wheat germ TFIIA to homogeneity and demonstrated that it substitutes for human TFIIA in a human i...

journal_title：Biochemical and biophysical research communications

authors： Yu XB,Burke C,Zhang J,Marchitelli LJ,Davis EA,Ackerman S

更新日期：1990-04-30 00:00:00

abstract：:Multidrug resistance in human tumour cells is often associated with increased expression of the 190kDa multidrug resistance protein, MRP1, that belongs to the ATP-binding cassette superfamily of transport proteins. MRP1 is also an efficient transporter of many organic anions. In the present study, we have mapped the e...

journal_title：Biochemical and biophysical research communications

authors： Koike K,Deeley RG,Cole SP

更新日期：2004-03-12 00:00:00

abstract：:The use of combined hormone replacement therapy (HRT) with oestrogens and progestins in postmenopausal women has been associated with an increased risk for developing breast cancer. The reasons are not fully understood, but influence of HRT on endogenous conversion of female sex hormones may be involved. The expressio...

journal_title：Biochemical and biophysical research communications

authors： Sivik T,Jansson A

更新日期：2012-05-25 00:00:00

abstract：:We demonstrate that the 204 bp intronic gene fragment of C3(1), which has a specific in vitro affinity for the androgen receptor, is able to confer androgen responsiveness to a heterologous promoter. This characteristic is completely destroyed by a single G----T substitution, affecting a 5'-TGTTCT-3' element that clos...

journal_title：Biochemical and biophysical research communications

authors： Claessens F,Celis L,Peeters B,Heyns W,Verhoeven G,Rombauts W

更新日期：1989-10-31 00:00:00

abstract：:A chemically synthesized 2,2,7-trimethylguanosine cap (TMG-cap) analogue was added to an in vitro pre-mRNA splicing system. A slight inhibition of the splicing was observed, but it was much less remarkable than that by a 7-methylguanosine cap analogue. This result does not favor a direct role of the TMG-cap structure ...

journal_title：Biochemical and biophysical research communications

authors： Tokumoto Y,Iwase R,Sekine M,Hata T,Ohshima Y

更新日期：1989-08-15 00:00:00

abstract：:Nanodisks (ND) are nanometer scale complexes of phospholipid and apolipoprotein that have been shown to function as drug delivery vehicles. ND harboring significant quantities of the antifungal agent, amphotericin B, or the bioactive isoprenoid, all trans retinoic acid, have been generated and characterized. As curren...

journal_title：Biochemical and biophysical research communications

authors： Iovannisci DM,Beckstead JA,Ryan RO

更新日期：2009-02-06 00:00:00

abstract：:A catechol 2,3-dioxygenase (C23O) gene was found from Sulfolobus solfataricus strain 98/2. Heterologous thermophilic C23O expressed in Escherichia coli showed the highest activity against catechol and 4-chlorocatechol, and at neutral pH. The C23O gene located with a putative multicomponent monooxygenase (MM) gene clus...

journal_title：Biochemical and biophysical research communications

authors： Chae JC,Kim E,Bini E,Zylstra GJ

更新日期：2007-06-08 00:00:00

abstract：:alpha-Crystallin, a major eye lens protein, has been shown to function like a molecular chaperone by suppressing the aggregation of other proteins induced by various stress conditions. Ultraviolet (UV) radiation is known to cause structural and functional alterations in the lens macromolecules. Earlier we observed tha...

journal_title：Biochemical and biophysical research communications

authors： Reddy GB,Reddy PY,Suryanarayana P

更新日期：2001-04-06 00:00:00

abstract：:The effect of thyroid-stimulating hormone receptor (TSHR) on hepatic lipid accumulation in vivo is not fully understood. Further, while TSHR in the thyroid has been studied extensively, whether and how the absence of TSHR in the liver affects systemic energy metabolism has not yet been reported. To examine these effec...

journal_title：Biochemical and biophysical research communications

authors： Zhou L,Wu K,Zhang L,Gao L,Chen S

更新日期：2018-02-26 00:00:00

abstract：:Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer fa...

journal_title：Biochemical and biophysical research communications

authors： Rosfjord E,Rizzino A

更新日期：1994-09-30 00:00:00

abstract：:Rat type 1 angiotensin II receptor has two subtypes, namely type 1a and type 1b. The regulation of the expressions of these two subtype receptor mRNAs was studied by using a competitive polymerase chain reaction method. The expression of the type 1a mRNA in the liver was negatively and that of the type 1b mRNA in the ...

journal_title：Biochemical and biophysical research communications

authors： Iwai N,Inagami T,Ohmichi N,Nakamura Y,Saeki Y,Kinoshita M

更新日期：1992-10-15 00:00:00

abstract：:High level of palmitate is associated with metabolic disorders. We recently showed that enhanced level of S-palmitoylated cytosolic thioredoxin (Trx1) in mouse liver was new characteristic feature of insulin resistance. However, our understanding of the effect of S-palmitoylation on Trx1 is limited, and the tissue spe...

journal_title：Biochemical and biophysical research communications

authors： Xu Z,Zhong L

更新日期：2015-05-15 00:00:00

abstract：:Autophagy is a mechanism to digest cells' own components, and its importance in many physiological and pathological processes is being recognized. But the molecular mechanism that regulates autophagy is not understood in detail. In the present study, we found that cholesterol depletion induces macroautophagy. The cell...

journal_title：Biochemical and biophysical research communications

authors： Cheng J,Ohsaki Y,Tauchi-Sato K,Fujita A,Fujimoto T

更新日期：2006-12-08 00:00:00

abstract：:The homogeneous recombinant mammalian protein tyrosine phosphatase 1B (PTP1B) and Yersinia protein tyrosine phosphatase (PTPase) are inactivated by a series of low-molecular-weight S-nitrosothiols. These compounds exhibited different inhibitory activities in a time- and concentration-dependent manner with second-order...

journal_title：Biochemical and biophysical research communications

authors： Xian M,Wang K,Chen X,Hou Y,McGill A,Zhou B,Zhang ZY,Cheng JP,Wang PG

更新日期：2000-02-16 00:00:00

abstract：:Antibody-based targeted therapy of cancers requires the antibody targeting of specific molecules inducing tumor cells apoptosis or death. Angiopoietin-2 (Agn-2) and translocator protein (TSPO) are identified as potential target molecules for glioblastoma therapy. The single chain anti-Agn-2 antibody (Anag-2) and anti-...

journal_title：Biochemical and biophysical research communications

authors： Li J,Zhang Z,Lv L,Qiao H,Chen X,Zou C

更新日期：2016-04-01 00:00:00

abstract：:Activation of seven-transmembrane G-protein coupled receptor (GPCR) mediates extracellular signals into intracellular responses. G-protein coupled receptor 55 (GPR55) is one of GPCRs and activated by endogenous cannabinoids. A family of regulators of G-protein signaling (RGS) stimulates GTP hydrolysis of alpha subunit...

journal_title：Biochemical and biophysical research communications

authors： Jang H,Kim Y,Ghil S

更新日期：2020-12-17 00:00:00