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Pre-mRNA with a trimethylguanosine cap structure can be spliced efficiently in vitro.

Abstract：

:A chemically synthesized 2,2,7-trimethylguanosine cap (TMG-cap) analogue was added to an in vitro pre-mRNA splicing system. A slight inhibition of the splicing was observed, but it was much less remarkable than that by a 7-methylguanosine cap analogue. This result does not favor a direct role of the TMG-cap structure of small nuclear RNAs in pre-mRNA splicing, and suggests that the TMG-cap does not effectively interact with the 7-methylguanosine cap binding factors. Using the SP6 in vitro transcription system and a TMG-cap analogue, we prepared a beta-globin transcript which has a TMG-cap at the 5' end. This TMG-capped transcript was spliced accurately and efficiently in vitro.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Tokumoto Y,Iwase R,Sekine M,Hata T,Ohshima Y

doi

10.1016/0006-291x(89)90769-9

subject

Has Abstract

pub_date

1989-08-15 00:00:00

pages

977-83

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(89)90769-9

journal_volume

162

pub_type

杂志文章

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