DNA ploidy analysis and cell proliferation in congenital sacrococcygeal teratomas.

Abstract:

BACKGROUND:Congenital sacrococcygeal teratoma is the most common germ cell tumor in infants and children. It usually is diagnosed at birth, is benign, and consists of fully differentiated mature tissues. Congenital sacrococcygeal teratomas (SCTs) also may contain immature tissues, most commonly of neural origin. The proportion of malignant teratomas increases with advancing age, but the relation between mature and immature SCTs is not well understood. Thus, it is very important to determine proliferative activity, DNA ploidy, and DNA index to predict biologic behavior of these tumors. METHODS:DNA ploidy and cell proliferation were analyzed by flow cytometry, and the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 were analyzed immunohistochemically on paraffin embedded tissue. RESULTS:All the tumors that were surgically treated within 3 months after birth, including immature teratoma, were diploid. Strongly positive PCNA immunostaining was found in both immature teratomas, and weakly positive PCNA was found in nine cases. Weak positivity for Ki-67 was observed in 2 cases, and moderate positivity was observed in 6 cases including immature teratomas. CONCLUSION:The value of flow cytometry in the prediction of biologic behavior of congenital SCT should be analyzed further. Our results suggest that Ki-67 and especially PCNA may reflect the proliferative activity of these tumors.

journal_name

Cancer

journal_title

Cancer

authors

Kruslin B,Visnjić A,Cizmic A,Tomicić I,Kos M,Jukić S,Seiwerth S

doi

10.1002/1097-0142(20000815)89:4<932::aid-cncr29>3.

subject

Has Abstract

pub_date

2000-08-15 00:00:00

pages

932-7

issue

4

eissn

0008-543X

issn

1097-0142

pii

10.1002/1097-0142(20000815)89:4<932::AID-CNCR29>3.

journal_volume

89

pub_type

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