The use of flow cytometry for the detection of CMV-specific antigen (pp65) in leukocytes of kidney recipients.

Abstract:

:Flow cytometric assay (FCA) was used to detect a cytomegalovirus (CMV) specific antigen (pp65) in CMV-infected fibroblast cells and in leukocytes of kidney recipients. FCA distinguished clearly between the infected and non-infected fibroblast cells. Regarding transplant patients, the FCA was positive when the number of antigenemia assay (AA) positive cells was five or more per 5 x 10(4). Moreover, the percentage of antigenemia-positive cells by FCA correlated well with symptomatic CMV infections.

journal_name

Clin Transplant

journal_title

Clinical transplantation

authors

Essa S,Pacsa AS,Al-Attiyah R,El-Shazly A,Raghupathy R,Said T

doi

10.1034/j.1399-0012.2000.140208.x

subject

Has Abstract

pub_date

2000-04-01 00:00:00

pages

147-51

issue

2

eissn

0902-0063

issn

1399-0012

journal_volume

14

pub_type

杂志文章
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