Donor CD163 and nestin-positive cells predict graft function in living donor liver transplant.

Abstract:

AIMS:To evaluate the effect of donor liver resident mesenchymal cells, M2 macrophages on liver graft outcome after living donor liver transplantation. MATERIALS AND METHODS:Seventy donor biopsies were included in the study. Outcomes at day 3, 7, 30, and 180 postliver transplantation were assessed. Mesenchymal stem cells and M2 macrophages in donor liver biopsies were evaluated. RESULTS:Mean age of recipients was 40.9 ± 13.6 years. Sex mismatched transplants were 44 (M→F = 9; F→M = 35). On area under receiver operative curve analysis, donor biopsy (DB) nestin ≥3 and CD 163 ≥ 32/200x at day 3; CD163 ≥ 32 at day 7; CD 163 > 32, pRBC of <6.5 units at day 30, and DB nestin ≥3, CD 163 ≥ 32 and pRBC<6.5 units at day 180 predicted adequate graft functions. On multivariate analysis, higher DB nestin (P = .009) and lower cryoprecipitate (P = .009) usage at day 3, higher DB CD163 (P = .006) at day 7, higher DB CD163 (P = .018) and reduced transfusion of packed cell (pRBC) (P = .014) at day 30 and higher DB nestin (P = .011), higher CD163 (P = .009), and reduced pRBC (P = .045) at day 180 were the predictors of better outcome. CONCLUSIONS:Donor liver biopsy nestin+ and CD163+ can predict early graft outcome in living donor liver transplantation.

journal_name

Clin Transplant

journal_title

Clinical transplantation

authors

Nigam N,Bihari C,Lal D,Rastogi A,Kumar S,Pamecha V,Kaur S,Kumar A,Sarin SK

doi

10.1111/ctr.13197

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

e13197

issue

3

eissn

0902-0063

issn

1399-0012

journal_volume

32

pub_type

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