Abstract:
BACKGROUND:Prevention of Helicobacter pylori infection may help to control related gastritis, peptic ulcer and cancer. Of the possible preventive measures, immunization was successfully employed in various animal studies. However, no immunization protocol has been accepted for humans. A better characterization of the immune response against the pathogen may be required before a human vaccine is developed. AIM:To identify bacterial proteins which induce an immune response in infected humans or H. pylori-immunized rabbits. METHODS:An expression library of H. pylori genes was screened with sera from infected humans and from immunized rabbits. Positive clones were partially sequenced and identified on the basis of a homology search of a H. pylori genome database. Encoded proteins were expressed directly from positive clones and analyzed by SDS-PAGE/Western blot techniques. RESULTS:114 positive clones were isolated: 79 by screening with human sera and 35 by screening with rabbit sera. Western blot analysis demonstrated that selected clones encoded one or more strongly immunoreactive proteins. 64 clones selected with human sera had no counterparts among clones from screening with rabbit serum. 13 of these clones encoded a total of 21 unknown H. pylori proteins. 17 clones selected with rabbit sera were not immunostained with human sera. They represent 2 various regions of the H. pylori genome which encoded 3 bacterial proteins of unknown function. CONCLUSIONS:Screening of H. pylori expression library identified immunogenic proteins - potential vaccine antigens.
journal_name
Digestionjournal_title
Digestionauthors
Lazowska I,Trzeciak L,Godlewska R,Hennig E,Jagusztyn-Krynicka K,Popowski J,Regula J,Ostrowski Jdoi
10.1159/000007731subject
Has Abstractpub_date
2000-01-01 00:00:00pages
14-21issue
1eissn
0012-2823issn
1421-9867pii
7731journal_volume
61pub_type
杂志文章相关文献
DIGESTION文献大全abstract:BACKGROUND:A patency capsule (PC) can help predict capsule endoscope (CE) retention; however, PC tolerability is unknown in children. We retrospectively evaluated PC tolerability in school-aged children. METHODS:Sixty-one patients (median age, 12.9 years; range 7.4-17.3 years) who underwent PC examination were analyze...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000477579
更新日期:2017-01-01 00:00:00
abstract::The case of a 52-year-old male with chronic active type B hepatitis in whom severe exacerbation of liver disease was associated with interferon alpha treatment is described. It seems that patients with very active forms of chronic hepatitis B may experience severe and symptomatic exacerbation of liver disease during i...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000200940
更新日期:1992-01-01 00:00:00
abstract::The prognosis for the acute healing of patients with persistent, erosive or ulcerative reflux oesophagitis depends upon the choice of drug treatment as well as the severity of the initial oesophagitis. The results of this study show that omeprazole, 40 mg once daily (n = 51), is more effective than ranitidine, 300 mg ...
journal_title:Digestion
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1159/000200522
更新日期:1990-01-01 00:00:00
abstract::The potential therapeutic applications of somatostatin and octreotide in gastroenterology involve gut neuro-endocrine tumours, bleeding varices, bleeding peptic ulcers, gastro-intestinal fistulae, pancreatic fistulae, dumping syndrome, pancreatic pseudocysts, short bowel syndrome, acute pancreatitis, AIDS-related diar...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000201079
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND/AIMS:In this study, a clinical and serological follow-up of 8-10 years was performed in relatives of celiac disease (CD) patients from southern Brazil. The occurrence of new CD cases in the families and the use of two different IgA-tTG enzyme-linked immunosorbent assay (ELISA) kits were also evaluated. METH...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000320451
更新日期:2011-01-01 00:00:00
abstract::Germ-free and conventional rats were fed a semi-synthetic diet including 10% wheat bran for 1 month. They were compared to similar rats fed the same diet without bran. Transit of polyethylene glycol (PEG) 4000 was studied in giving the marker either in saline or in the diet. The presence of a bacterial flora was found...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000199006
更新日期:1984-01-01 00:00:00
abstract::Chemiluminescence (CL) is a simple quantitative assay of polymorphonuclear leucocyte (PMNL) oxidative metabolism. PMNL CL was found to be significantly higher in patients with chronic inflammatory bowel disease than in normal controls (167 +/- 60 vs. 139 +/- 50 mV/10(5) cells, p less than 0.05). There were no signific...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000200238
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND/AIMS:People may have symptoms of multiple disorders at the same time. We aimed to determine prevalence and risk factors for overlaps between gastroesophageal reflux disease (GERD), dyspepsia and irritable bowel syndrome (IBS) in a Korean population. METHODS:A cross-sectional survey was performed on 1,688 ra...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000211715
更新日期:2009-01-01 00:00:00
abstract:INTRODUCTION:The incidence of early-onset inflammatory bowel disease is increasing in Japan. OBJECTIVE:This study aimed to analyze the treatment and progress of early-onset inflammatory bowel disease. METHODS:This prospective survey evaluated the data of 43 patients aged <8 years who were diagnosed with inflammatory ...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000507570
更新日期:2020-05-18 00:00:00
abstract:BACKGROUND AND AIM:Sonic hedgehog (Shh) is a morphogen involved in the homeostasis of the gastric fundic glands. Alterations of gastric mucosal Shh expression after eradication of Helicobacter pylori were examined. METHOD:Mongolian gerbils were inoculated with H. pylori at the age of 5 weeks. H. pylori eradication was...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000209219
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND/AIMS:While probiotic bacteria are successfully used in the treatment of ulcerative colitis, the effect of commercially available probiotic products is still controversial. Here, we study whether the number of living probiotic bacteria in yoghurts is altered by an interruption of the cold chain. METHODS:Thre...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000308715
更新日期:2011-01-01 00:00:00
abstract::Since arginine can stimulate lymphocyte proliferation in the healthy human, its effect on lymphocyte proliferation in vitro was studied in 7 patients with far advanced gastric cancer. These patients with normal nourishment were ambulatory and could consume a regular diet. A daily dietary supplement of 30 g arginine fo...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000201023
更新日期:1993-01-01 00:00:00
abstract::Lithium is a cation which has central nervous system effects and is able to interfere with both the release and action of polypeptide hormones. We examined its effect on basal and stimulated plasma gastrin and pancreatic polypeptide (PP) levels in 4 dogs with chronic gastric fistulas. Gastrin and PP release were stimu...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000198642
更新日期:1981-01-01 00:00:00
abstract::This prospective study was designed to validate a novel biopsy urease test as well as a simplified 13C-urea breath test for the detection of Helicobacter pylori. In addition, the hypothesis was tested that both the reaction velocity of the urease test and the 13CO2 excess of the urea breath test may allow a prediction...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000201366
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND/AIMS:Although considerable information exists regarding gastroesophageal reflux disease with erosions, much less is known of non-erosive reflux disease (NERD), the dominant form of reflux disease in the developed world. METHODS:An expert international group using the modified Delphi technique examined the q...
journal_title:Digestion
pub_type: 共识发展会议,杂志文章
doi:10.1159/000219365
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:The combined effects of nutrient malabsorption and adiposity on vitamin D status are unclear in pediatric malabsorption syndromes. AIM:To determine the relationship between adiposity and serum 25-hydroxyvitamin D (25(OH)D) in malabsorption disorders. METHODS:Prepubertal children of ages 3-12 with either la...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000381895
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND/AIMS:Evaluation of the response of gastroesophageal reflux disease (GERD) symptoms to treatment would be facilitated by a brief, valid, reliable and responsive, self-assessed GERD-sensitive scale. We therefore developed the Reflux Questionnaire (ReQuest). This publication describes the psychometric evaluatio...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000079708
更新日期:2004-01-01 00:00:00
abstract::This study examined the morphological and functional adaptations occurring in the jejunum of adult rats fed totally by parenteral nutrition during a 4-day period. Comparison was made with sham-operated animals receiving orally a similar isocaloric diet. The intravenously fed rats exhibited a 20% shortening of the vill...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000199030
更新日期:1984-01-01 00:00:00
abstract::The effect of the new antiulcer agent plaunotol on the release of endogenous secretin and the pancreatic exocrine secretion was investigated in anesthetized rats. In 48 rats with pancreatic and biliary diversion, continuous intraduodenal infusion of plaunotol in 3 graded doses (5, 20 and 80 mg/h/rat) resulted in signi...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000199903
更新日期:1989-01-01 00:00:00
abstract::Somatostatin (SST) has been shown to induce cholestasis in the dog and in the rat. In man, it is still unknown whether SST modifies bile formation. The present study was undertaken to examine the influence of SST on bile secretion in man. Two volunteers who had a total external biliary fistula received 1-hour SST infu...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000199820
更新日期:1989-01-01 00:00:00
abstract:INTRODUCTION:Multiple Lugol-voiding lesions (LVLs) in the esophagus increase the risk of synchronous and metachronous development of esophageal squamous cell carcinoma (ESCC). Chemoradiotherapy (CRT) following endoscopic submucosal dissection (ESD) may reduce the incidence of metachronous ESCC, but few studies have inv...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000510368
更新日期:2020-09-29 00:00:00
abstract:BACKGROUND:Neoadjuvant chemotherapy for advanced gastric cancer is expected to improve prognoses. However, as there is no method to evaluate neoadjuvant chemotherapeutic efficacy before gastrectomy, some patients at high risk for a poor prognosis undergo gastrectomy. The aim of the present study was to investigate whet...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000500907
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND/AIMS:The changes in gastroesophageal reflux disease (GERD)-related symptoms on treatment are variously described, but currently available questionnaires have shortcomings. We therefore developed a self-assessment reflux questionnaire (ReQuest). This article describes the process of development and testing. ...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000101080
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND/AIMS:Acotiamide, a prokinetic drug, is used to treat functional dyspepsia (FD), especially postprandial distress syndrome (PDS). However, a treatment for FD patients with PDS and/or epigastric pain syndrome (EPS) has not been established. We investigated the efficacy of famotidine in combination with acotiam...
journal_title:Digestion
pub_type: 杂志文章,随机对照试验
doi:10.1159/000477345
更新日期:2017-01-01 00:00:00
abstract::Life-long administration (greater than or equal to 2 years) of a number of long-acting gastric acid inhibitors (including H2-receptor antagonists, omeprazole and ciprofibrate) has been associated with the development of gastric enterochromaffin-like cell (ECL cell) carcinoids in rats. It has been postulated that they ...
journal_title:Digestion
pub_type: 杂志文章,评审
doi:10.1159/000200510
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Determining the depth of invasion is important when considering therapeutic strategies for early gastric cancer (EGC). We determined the effects of learning the non-extension sign, that is, an index of T1b2 in EGC, on identifying its depth of invasion. METHODS:Endoscopic images of 40 EGC cases (20 showing p...
journal_title:Digestion
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1159/000498845
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Endoscopic submucosal dissection (ESD) is recognized as a minimally invasive and curative treatment for superficial gastrointestinal (GI) cancers. However, ESD is still challenging and time-consuming with a high risk of adverse events such as bleeding and perforation. Various traction methods have been explo...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000511731
更新日期:2020-12-22 00:00:00
abstract::Chornic exogenous administration of cholecystokinin octapeptide (CCK8) to rats led to a reduced sensitivity of pancreatic acinar cells to both CCK8 and carbachol stimulation without changes in affinity or number of CCK or muscarinic receptors. In addition, repeated feeding of camostate, a synthetic protease inhibitor ...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000200386
更新日期:1990-01-01 00:00:00
abstract::Autonomic hyperactivity with increased cardiac output and other manifestations occurs in patients with both cirrhosis and noncirrhotic portal hypertension. The role of the disordered hepatic autonomic innervation in the mediation of these abnormalities is unknown. We have studied the hepatic nerve network and associat...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000201156
更新日期:1994-01-01 00:00:00
abstract::Life events and daily stress were assessed in patients with duodenal ulcer, active or in remission, and in matched disease and normal controls. The mean positive life event scores were significantly lower in duodenal ulcer patients than in disease and healthy controls, and lower in patients with active ulcer than in t...
journal_title:Digestion
pub_type: 杂志文章
doi:10.1159/000200949
更新日期:1992-01-01 00:00:00