In search of immunogenic Helicobacter pylori proteins by screening of expression library.

Abstract:

BACKGROUND:Prevention of Helicobacter pylori infection may help to control related gastritis, peptic ulcer and cancer. Of the possible preventive measures, immunization was successfully employed in various animal studies. However, no immunization protocol has been accepted for humans. A better characterization of the immune response against the pathogen may be required before a human vaccine is developed. AIM:To identify bacterial proteins which induce an immune response in infected humans or H. pylori-immunized rabbits. METHODS:An expression library of H. pylori genes was screened with sera from infected humans and from immunized rabbits. Positive clones were partially sequenced and identified on the basis of a homology search of a H. pylori genome database. Encoded proteins were expressed directly from positive clones and analyzed by SDS-PAGE/Western blot techniques. RESULTS:114 positive clones were isolated: 79 by screening with human sera and 35 by screening with rabbit sera. Western blot analysis demonstrated that selected clones encoded one or more strongly immunoreactive proteins. 64 clones selected with human sera had no counterparts among clones from screening with rabbit serum. 13 of these clones encoded a total of 21 unknown H. pylori proteins. 17 clones selected with rabbit sera were not immunostained with human sera. They represent 2 various regions of the H. pylori genome which encoded 3 bacterial proteins of unknown function. CONCLUSIONS:Screening of H. pylori expression library identified immunogenic proteins - potential vaccine antigens.

journal_name

Digestion

journal_title

Digestion

authors

Lazowska I,Trzeciak L,Godlewska R,Hennig E,Jagusztyn-Krynicka K,Popowski J,Regula J,Ostrowski J

doi

10.1159/000007731

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

14-21

issue

1

eissn

0012-2823

issn

1421-9867

pii

7731

journal_volume

61

pub_type

杂志文章
  • Patency Capsule Tolerability in School-Aged Children.

    abstract:BACKGROUND:A patency capsule (PC) can help predict capsule endoscope (CE) retention; however, PC tolerability is unknown in children. We retrospectively evaluated PC tolerability in school-aged children. METHODS:Sixty-one patients (median age, 12.9 years; range 7.4-17.3 years) who underwent PC examination were analyze...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000477579

    authors: Tokuhara D,Watanabe K,Cho Y,Shintaku H

    更新日期:2017-01-01 00:00:00

  • Severe exacerbation of chronic active hepatitis B during interferon alpha therapy.

    abstract::The case of a 52-year-old male with chronic active type B hepatitis in whom severe exacerbation of liver disease was associated with interferon alpha treatment is described. It seems that patients with very active forms of chronic hepatitis B may experience severe and symptomatic exacerbation of liver disease during i...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000200940

    authors: Laskus T,Radkowski M,Slusarczyk J,Cianciara J

    更新日期:1992-01-01 00:00:00

  • Prevention of relapse of reflux oesophagitis after endoscopic healing: the efficacy and safety of omeprazole compared with ranitidine.

    abstract::The prognosis for the acute healing of patients with persistent, erosive or ulcerative reflux oesophagitis depends upon the choice of drug treatment as well as the severity of the initial oesophagitis. The results of this study show that omeprazole, 40 mg once daily (n = 51), is more effective than ranitidine, 300 mg ...

    journal_title:Digestion

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1159/000200522

    authors: Lundell L

    更新日期:1990-01-01 00:00:00

  • Potential indications for octreotide in gastroenterology: summary of workshop.

    abstract::The potential therapeutic applications of somatostatin and octreotide in gastroenterology involve gut neuro-endocrine tumours, bleeding varices, bleeding peptic ulcers, gastro-intestinal fistulae, pancreatic fistulae, dumping syndrome, pancreatic pseudocysts, short bowel syndrome, acute pancreatitis, AIDS-related diar...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000201079

    authors: Burroughs AK,Malagelada R

    更新日期:1993-01-01 00:00:00

  • Serological and clinical follow-up of relatives of celiac disease patients from southern Brazil.

    abstract:BACKGROUND/AIMS:In this study, a clinical and serological follow-up of 8-10 years was performed in relatives of celiac disease (CD) patients from southern Brazil. The occurrence of new CD cases in the families and the use of two different IgA-tTG enzyme-linked immunosorbent assay (ELISA) kits were also evaluated. METH...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000320451

    authors: Nass FR,Kotze LM,Nisihara RM,de Messias-Reason IJ,Ramos da Rosa Utiyama S

    更新日期:2011-01-01 00:00:00

  • Effect of wheat bran upon gastro-intestinal transit in germ-free and conventional rats.

    abstract::Germ-free and conventional rats were fed a semi-synthetic diet including 10% wheat bran for 1 month. They were compared to similar rats fed the same diet without bran. Transit of polyethylene glycol (PEG) 4000 was studied in giving the marker either in saline or in the diet. The presence of a bacterial flora was found...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000199006

    authors: Riottot M,Sacquet E,Leprince C

    更新日期:1984-01-01 00:00:00

  • Chemiluminescence by polymorphonuclear leucocyte subpopulations in chronic inflammatory bowel disease. Influence of the cell separation procedure.

    abstract::Chemiluminescence (CL) is a simple quantitative assay of polymorphonuclear leucocyte (PMNL) oxidative metabolism. PMNL CL was found to be significantly higher in patients with chronic inflammatory bowel disease than in normal controls (167 +/- 60 vs. 139 +/- 50 mV/10(5) cells, p less than 0.05). There were no signific...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000200238

    authors: Kelleher D,Feighery C,Weir DG

    更新日期:1990-01-01 00:00:00

  • Prevalence and risk factors for overlaps between gastroesophageal reflux disease, dyspepsia, and irritable bowel syndrome: a population-based study.

    abstract:BACKGROUND/AIMS:People may have symptoms of multiple disorders at the same time. We aimed to determine prevalence and risk factors for overlaps between gastroesophageal reflux disease (GERD), dyspepsia and irritable bowel syndrome (IBS) in a Korean population. METHODS:A cross-sectional survey was performed on 1,688 ra...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000211715

    authors: Lee SY,Lee KJ,Kim SJ,Cho SW

    更新日期:2009-01-01 00:00:00

  • A Multicenter Prospective Survey on Early-Onset Inflammatory Bowel Disease in Japan.

    abstract:INTRODUCTION:The incidence of early-onset inflammatory bowel disease is increasing in Japan. OBJECTIVE:This study aimed to analyze the treatment and progress of early-onset inflammatory bowel disease. METHODS:This prospective survey evaluated the data of 43 patients aged <8 years who were diagnosed with inflammatory ...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000507570

    authors: Kudo T,Fujii T,Maisawa SI,Sasaki M,Uchida K,Ida S,Kagimoto S,Yoden A,Shimizu T

    更新日期:2020-05-18 00:00:00

  • Early Helicobacter pylori eradication restores sonic hedgehog expression in the gastric mucosa of Mongolian gerbils.

    abstract:BACKGROUND AND AIM:Sonic hedgehog (Shh) is a morphogen involved in the homeostasis of the gastric fundic glands. Alterations of gastric mucosal Shh expression after eradication of Helicobacter pylori were examined. METHOD:Mongolian gerbils were inoculated with H. pylori at the age of 5 weeks. H. pylori eradication was...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000209219

    authors: Nishizawa T,Suzuki H,Nakagawa I,Minegishi Y,Masaoka T,Iwasaki E,Hibi T

    更新日期:2009-01-01 00:00:00

  • Dying in yoghurt: the number of living bacteria in probiotic yoghurt decreases under exposure to room temperature.

    abstract:BACKGROUND/AIMS:While probiotic bacteria are successfully used in the treatment of ulcerative colitis, the effect of commercially available probiotic products is still controversial. Here, we study whether the number of living probiotic bacteria in yoghurts is altered by an interruption of the cold chain. METHODS:Thre...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000308715

    authors: Scharl M,Geisel S,Vavricka SR,Rogler G

    更新日期:2011-01-01 00:00:00

  • Can daily dietary arginine supplement affect the function and subpopulation of lymphocytes in patients with advanced gastric cancer?

    abstract::Since arginine can stimulate lymphocyte proliferation in the healthy human, its effect on lymphocyte proliferation in vitro was studied in 7 patients with far advanced gastric cancer. These patients with normal nourishment were ambulatory and could consume a regular diet. A daily dietary supplement of 30 g arginine fo...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000201023

    authors: Wu CW,Chi CW,Chiu CC,Wu HS,Liu WY,P'eng FK,Wang SR

    更新日期:1993-01-01 00:00:00

  • Variable effect of lithium on canine pancreatic polypeptide and gastrin release by a meal or bombesin.

    abstract::Lithium is a cation which has central nervous system effects and is able to interfere with both the release and action of polypeptide hormones. We examined its effect on basal and stimulated plasma gastrin and pancreatic polypeptide (PP) levels in 4 dogs with chronic gastric fistulas. Gastrin and PP release were stimu...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000198642

    authors: Modlin IM,Ehrlich BE,Lamers CB,Diamond JM,Walsh JH

    更新日期:1981-01-01 00:00:00

  • Validity of a novel biopsy urease test (HUT) and a simplified 13C-urea breath test for diagnosis of Helicobacter pylori infection and estimation of the severity of gastritis.

    abstract::This prospective study was designed to validate a novel biopsy urease test as well as a simplified 13C-urea breath test for the detection of Helicobacter pylori. In addition, the hypothesis was tested that both the reaction velocity of the urease test and the 13CO2 excess of the urea breath test may allow a prediction...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000201366

    authors: Labenz J,Bärsch G,Peitz U,Aygen S,Hennemann O,Tillenburg B,Becker T,Stolte M

    更新日期:1996-11-01 00:00:00

  • Diagnosis and management of non-erosive reflux disease--the Vevey NERD Consensus Group.

    abstract:BACKGROUND/AIMS:Although considerable information exists regarding gastroesophageal reflux disease with erosions, much less is known of non-erosive reflux disease (NERD), the dominant form of reflux disease in the developed world. METHODS:An expert international group using the modified Delphi technique examined the q...

    journal_title:Digestion

    pub_type: 共识发展会议,杂志文章

    doi:10.1159/000219365

    authors: Modlin IM,Hunt RH,Malfertheiner P,Moayyedi P,Quigley EM,Tytgat GN,Tack J,Heading RC,Holtman G,Moss SF,Vevey NERD Consensus Group.

    更新日期:2009-01-01 00:00:00

  • Vitamin D Status and Adiposity in Pediatric Malabsorption Syndromes.

    abstract:BACKGROUND:The combined effects of nutrient malabsorption and adiposity on vitamin D status are unclear in pediatric malabsorption syndromes. AIM:To determine the relationship between adiposity and serum 25-hydroxyvitamin D (25(OH)D) in malabsorption disorders. METHODS:Prepubertal children of ages 3-12 with either la...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000381895

    authors: Nwosu BU,Maranda L

    更新日期:2015-01-01 00:00:00

  • Evaluation of GERD symptoms during therapy. Part II. Psychometric evaluation and validation of the new questionnaire ReQuest in erosive GERD.

    abstract:BACKGROUND/AIMS:Evaluation of the response of gastroesophageal reflux disease (GERD) symptoms to treatment would be facilitated by a brief, valid, reliable and responsive, self-assessed GERD-sensitive scale. We therefore developed the Reflux Questionnaire (ReQuest). This publication describes the psychometric evaluatio...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000079708

    authors: Mönnikes H,Bardhan KD,Stanghellini V,Berghöfer P,Bethke TD,Armstrong D

    更新日期:2004-01-01 00:00:00

  • Stimulation of disaccharidase activities in the jejunal brush border membrane of adult rat by total parenteral nutrition. Effects of thyroid hormones.

    abstract::This study examined the morphological and functional adaptations occurring in the jejunum of adult rats fed totally by parenteral nutrition during a 4-day period. Comparison was made with sham-operated animals receiving orally a similar isocaloric diet. The intravenously fed rats exhibited a 20% shortening of the vill...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000199030

    authors: Raul F,Galluser M,Doffoel M

    更新日期:1984-01-01 00:00:00

  • Plaunotol stimulates endogenous secretin release and exocrine pancreatic secretion in rats.

    abstract::The effect of the new antiulcer agent plaunotol on the release of endogenous secretin and the pancreatic exocrine secretion was investigated in anesthetized rats. In 48 rats with pancreatic and biliary diversion, continuous intraduodenal infusion of plaunotol in 3 graded doses (5, 20 and 80 mg/h/rat) resulted in signi...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000199903

    authors: Chang JH,Watanabe S,Shiratori K,Moriyoshi Y,Takeuchi T

    更新日期:1989-01-01 00:00:00

  • Pharmacological effect of somatostatin on bile secretion in man.

    abstract::Somatostatin (SST) has been shown to induce cholestasis in the dog and in the rat. In man, it is still unknown whether SST modifies bile formation. The present study was undertaken to examine the influence of SST on bile secretion in man. Two volunteers who had a total external biliary fistula received 1-hour SST infu...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000199820

    authors: Marteau P,Chrétien Y,Calmus Y,Parc R,Poupon R

    更新日期:1989-01-01 00:00:00

  • Effectiveness of Chemoradiotherapy for Metachronous Esophageal Squamous Cell Carcinoma.

    abstract:INTRODUCTION:Multiple Lugol-voiding lesions (LVLs) in the esophagus increase the risk of synchronous and metachronous development of esophageal squamous cell carcinoma (ESCC). Chemoradiotherapy (CRT) following endoscopic submucosal dissection (ESD) may reduce the incidence of metachronous ESCC, but few studies have inv...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000510368

    authors: Suzuki Y,Kikuchi D,Hoteya S,Okamura T,Ochiai Y,Hayasaka J,Dan N,Mitsunaga Y,Tanaka M,Odagiri H,Nomura K,Yamashita S,Matsui A,Iizuka T

    更新日期:2020-09-29 00:00:00

  • Endoscopic Evaluation of Neoadjuvant Chemotherapeutic Efficacy in Gastric Cancer before Gastrectomy Might be as Useful as Histological Assessment after Gastrectomy.

    abstract:BACKGROUND:Neoadjuvant chemotherapy for advanced gastric cancer is expected to improve prognoses. However, as there is no method to evaluate neoadjuvant chemotherapeutic efficacy before gastrectomy, some patients at high risk for a poor prognosis undergo gastrectomy. The aim of the present study was to investigate whet...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000500907

    authors: Sugawara N,Ota K,Terazawa T,Tanaka R,Akutagawa H,Yamaguchi T,Imai Y,Harada S,Kojima Y,Tashiro K,Kii T,Lee SW,Kawai M,Egashira Y,Goto M,Uchiyama K,Hirose Y,Takeuchi T,Higuchi K

    更新日期:2020-01-01 00:00:00

  • Evaluation of GERD symptoms during therapy. Part I. Development of the new GERD questionnaire ReQuest.

    abstract:BACKGROUND/AIMS:The changes in gastroesophageal reflux disease (GERD)-related symptoms on treatment are variously described, but currently available questionnaires have shortcomings. We therefore developed a self-assessment reflux questionnaire (ReQuest). This article describes the process of development and testing. ...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000101080

    authors: Bardhan KD,Stanghellini V,Armstrong D,Berghöfer P,Gatz G,Mönnikes H

    更新日期:2007-01-01 00:00:00

  • A Randomized, Double-Blind, Pilot Study of the Effect of Famotidine on Acotiamide Treatment for Functional Dyspepsia.

    abstract:BACKGROUND/AIMS:Acotiamide, a prokinetic drug, is used to treat functional dyspepsia (FD), especially postprandial distress syndrome (PDS). However, a treatment for FD patients with PDS and/or epigastric pain syndrome (EPS) has not been established. We investigated the efficacy of famotidine in combination with acotiam...

    journal_title:Digestion

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000477345

    authors: Hojo M,Nagahara A,Asaoka D,Takeda T,Izumi K,Matsumoto K,Ueyama H,Shimada Y,Matsumoto K,Nojiri S,Watanabe S

    更新日期:2017-01-01 00:00:00

  • Gastrin and gastric enterochromaffin-like cell carcinoids in the rat.

    abstract::Life-long administration (greater than or equal to 2 years) of a number of long-acting gastric acid inhibitors (including H2-receptor antagonists, omeprazole and ciprofibrate) has been associated with the development of gastric enterochromaffin-like cell (ECL cell) carcinoids in rats. It has been postulated that they ...

    journal_title:Digestion

    pub_type: 杂志文章,评审

    doi:10.1159/000200510

    authors: Carlsson E,Havu N,Mattsson H,Ekman L

    更新日期:1990-01-01 00:00:00

  • Learning Effect of Diagnosing Depth of Invasion Using Non-Extension Sign in Early Gastric Cancer.

    abstract:BACKGROUND:Determining the depth of invasion is important when considering therapeutic strategies for early gastric cancer (EGC). We determined the effects of learning the non-extension sign, that is, an index of T1b2 in EGC, on identifying its depth of invasion. METHODS:Endoscopic images of 40 EGC cases (20 showing p...

    journal_title:Digestion

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1159/000498845

    authors: Takeda T,So S,Sakurai T,Nakamura S,Yoshikawa I,Yada S,Itaba S,Yamagata H,Akiho H,Takatsu N,Wada Y,Ohtsu K,Nagahama T,Yao K

    更新日期:2020-01-01 00:00:00

  • Endoscopic Submucosal Dissection Using EndoTrac, a Novel Traction Device.

    abstract:BACKGROUND:Endoscopic submucosal dissection (ESD) is recognized as a minimally invasive and curative treatment for superficial gastrointestinal (GI) cancers. However, ESD is still challenging and time-consuming with a high risk of adverse events such as bleeding and perforation. Various traction methods have been explo...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000511731

    authors: Kaku H,Toyonaga T,Tanaka S,Takihara H,Baba S,Tsubouchi E,Ikeda Y,Orita H,Nakamoto M,Horikawa Y,Chiba H,Ban H,Furumoto Y,Morita R,Kodama Y

    更新日期:2020-12-22 00:00:00

  • Intracellular mechanism responsible for reduced enzyme secretion from camostate-induced hypertrophied pancreas.

    abstract::Chornic exogenous administration of cholecystokinin octapeptide (CCK8) to rats led to a reduced sensitivity of pancreatic acinar cells to both CCK8 and carbachol stimulation without changes in affinity or number of CCK or muscarinic receptors. In addition, repeated feeding of camostate, a synthetic protease inhibitor ...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000200386

    authors: Herzig KH,May D,Louie DS,Takahashi T,Göke B,Owyang C

    更新日期:1990-01-01 00:00:00

  • Distribution of hepatic nerve fibers in liver diseases.

    abstract::Autonomic hyperactivity with increased cardiac output and other manifestations occurs in patients with both cirrhosis and noncirrhotic portal hypertension. The role of the disordered hepatic autonomic innervation in the mediation of these abnormalities is unknown. We have studied the hepatic nerve network and associat...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000201156

    authors: Jaskiewicz K,Voigt MD,Robson SC

    更新日期:1994-01-01 00:00:00

  • Life events and daily stress in duodenal ulcer disease. A prospective study of patients with active disease and in remission.

    abstract::Life events and daily stress were assessed in patients with duodenal ulcer, active or in remission, and in matched disease and normal controls. The mean positive life event scores were significantly lower in duodenal ulcer patients than in disease and healthy controls, and lower in patients with active ulcer than in t...

    journal_title:Digestion

    pub_type: 杂志文章

    doi:10.1159/000200949

    authors: Hui WM,Shiu LP,Lok AS,Lam SK

    更新日期:1992-01-01 00:00:00