Abstract:
:The regulation of insulin-like growth factor binding protein (IGFBP)-4 proteolytic degradation by insulin-like growth factors (IGFs) has been largely studied in vitro, but not in vivo. The aim of this study was to investigate the involvement of IGFs, IGFBP-2, IGFBP-3, and IGFBP-3 proteolytic fragments in the regulation of IGFBP-4 proteolytic activity in ovine ovarian follicles. Follicular fluid from preovulatory follicles contains proteolytic activity degrading exogenous IGFBP-4. The addition of an excess of IGF-I enhanced IGFBP-4 proteolytic degradation, whereas addition of IGFBP-2 or -3 or monoclonal antibodies against IGF-I and -II dose dependently inhibited IGFBP-4 proteolytic degradation. IGF-I and IGF-II, but not LongR3-IGF-I, reversed this inhibition in a dose-dependent manner. C-terminal, but not N-terminal, proteolytic fragments derived from IGFBP-3 (aa 161-264), as well as heparin-binding domain-containing peptides derived from the C-terminal domain of IGFBP-3 and -5 also induced the inhibition of IGFBP-4 proteolytic degradation. Other heparin-binding domain-containing peptides derived from the connective tissue growth factor (CTGF) and from proteins not related to IGFBP, heparan/heparin interacting protein (HIP) and vitronectin, but not from p36 subunit of annexin II tetramer, inhibited IGFBP-4 degradation. Furthermore, IGFBP-3, mutated on its heparin-binding domain, was not able to inhibit IGFBP-4 proteolytic degradation. So, in ovine preovulatory follicles, IGFBP-4 proteolytic degradation both 1) depends on IGFs, and 2) is inhibited by IGFBP-3 via its C-terminal heparin-binding domain as well as by heparin-binding domain containing peptides. These data suggest that in early atretic follicles, the increase in IGFBP-2 participates in the decrease in IGFBP-4 degradation. In late atretic follicles, the increase in the levels of C-terminal IGFBP-3 proteolytic fragments, generated by IGFBP-3 degradation, as well as the increase in IGFBP-5 expression would strengthen the inhibition of IGFBP-4 degradation. This inhibition might be partly mediated by direct interaction of IGFBP-4 proteinase(s) and heparin-binding domain within the C-terminal region from IGFBP-3 and -5.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Mazerbourg S,Zapf J,Bar RS,Brigstock DR,Lalou C,Binoux M,Monget Pdoi
10.1210/endo.140.9.6979subject
Has Abstractpub_date
1999-09-01 00:00:00pages
4175-84issue
9eissn
0013-7227issn
1945-7170journal_volume
140pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::IGF-binding protein-3 (IGFBP-3) is the major circulating carrier protein for IGF, and also acts as a potent antiproliferative agent in various cell types. Recently, IGFBP-3 was reported to mediate high glucose-induced apoptosis in mesangial cells and podocytes. In this study, we investigated the role of IGFBP-3 in hig...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1122
更新日期:2011-08-01 00:00:00
abstract::Steroid receptor activation in developing brain influences a variety of cellular processes that endure into adulthood, altering both behavior and physiology. We report that estrogen receptors can be activated in a ligand-independent manner within developing brain by membrane dopamine receptors. Neonatal treatment with...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0498
更新日期:2005-09-01 00:00:00
abstract::The SMRT coregulator functions as a dual coactivator and corepressor for estrogen receptor-α (ERα) in a gene-specific manner, and in several studies its elevated expression correlates with poor outcome for breast cancer patients. A specific role of SMRT in breast cancer progression has not been elucidated, but SMRT kn...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1002
更新日期:2014-09-01 00:00:00
abstract::Somatostatin (SRIH) inhibits cell proliferation by interacting with five distinct SRIH receptor subtypes (SSTRs) activating several pathways in many tissues. We previously demonstrated that SRIH, by activating Src homology-2-containing protein, inhibits cell proliferation of the human medullary thyroid carcinoma cell ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1479
更新日期:2006-07-01 00:00:00
abstract::The fate of cell-bound [125I]iodo-human GH ([125I]iodo-hGH) was studied in monolayer cultures of hepatocytes from pregnant and nonpregnant rats. Both the binding of [125I]iodo-hGH and its degradation were significantly higher in cells from pregnant than from nonpregnant rats. The positive correlation between the numbe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-5-1475
更新日期:1984-05-01 00:00:00
abstract::Although the increased expression of Igf-I in liver in response to GH is well characterized, the intracellular signaling pathways that mediate this effect have not been identified. Intracellular signaling molecules belonging to the Janus kinase-signal transducer and activator of transcription 5b (JAK2-STAT5b) pathway ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8400
更新日期:2001-09-01 00:00:00
abstract::We have recently demonstrated that GnRH-stimulated gonadotropin secretion is augmented by coadministration with neuropeptide-Y (NPY) in anterior pituitaries removed in the afternoon from proestrous, but not metestrous, rats. To test the hypothesis that these effects of NPY are due to an interaction with progesterone (...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.1.7828530
更新日期:1995-01-01 00:00:00
abstract::In previous studies we found that plasma angiotensinogen levels were reduced by lesions of the hypothalamic paraventricular nuclei. To determine if the decrease was caused by decreased secretion of hormones that normally stimulate angiotensinogen secretion by the liver, we correlated the changes in plasma angiotensino...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-901
更新日期:1991-08-01 00:00:00
abstract::Although the main role of prolactin (PRL) in pregnant rodents is to sustain progesterone production by the corpus luteum, progesterone treatment of PRL or PRL receptor (PRL-R) null mice is unable to prevent fetal loss. We have previously shown that the rat decidua is a site of PRL production and action. In this report...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1643
更新日期:2007-05-01 00:00:00
abstract::During the third week of postnatal life, dramatic ontogenic changes occur in the morphology and enzymology of the small intestine of the infant rat, enabling the animal to make the transition from milk to solid food. To investigate the roles of T4 and GH in regulation of these changes, infant rats were hypophysectomiz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-3-1417
更新日期:1991-09-01 00:00:00
abstract::Prenatal dexamethasone exposure (PDE) induces multiorgan developmental toxicities in offspring. Here we verified the transgenerational inheritance effect of ovarian developmental toxicity by PDE and explored its intrauterine programming mechanism. Pregnant rats subcutaneously received 0.2 mg/kg/d dexamethasone from ge...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00044
更新日期:2018-03-01 00:00:00
abstract::Obesity induced by high-fat (HF) feeding is associated with low-grade inflammation in peripheral tissues that predisposes to insulin resistance. Recent evidence suggests the occurrence of a similar process in the hypothalamus, which favors weight gain through impairment of leptin and insulin signaling. In addition to ...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2010-0336
更新日期:2010-09-01 00:00:00
abstract::To study conversion of 3,5,3'-triiodothyroinine (T3) and 3,3',5'-triiodothyronine (rT3) to 3,3'-diiodothyronine (T2) in vitro, T3 or rT3 was incubated at pH 7.35 with homogenates of several rat tissues (liver, kidney, muscle, heart, ling, spleen, intestines, and brain) for 15 min at 37 C. The T2 generated during incub...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-4-1099
更新日期:1978-04-01 00:00:00
abstract::The peptides angiotensin II (ANGII) and atrial natriuretic factor (ANF) regulate blood pressure and salt and water balance by producing antagonistic physiological effects in a variety of tissues. We used in vitro autoradiography with [125I] ANGII and [125I]ANF to compare receptor regulation for both peptides in variou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-2799
更新日期:1989-06-01 00:00:00
abstract::Basal serum gonadotropin levels were monitored weekly in female hamsters from birth to 10 weeks of age. Hamsters raised on three different photoperiods presented uniform pre- and postpubertal patterns of serum LH and FSH, suggesting that gonadotropin release in the young hamster occurs independently of ambient photope...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-4-1262
更新日期:1982-04-01 00:00:00
abstract::The nuclear orphan receptor human estrogen receptor-related receptor (ERR)-alpha is implicated in bone metabolism. We studied the effect of ERRalpha silencing in human mesenchymal stem cells (hMSCs) during osteoblastogenesis. We found that ERRalpha silencing led to an increase of bone sialoprotein and a decrease of os...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0121
更新日期:2009-10-01 00:00:00
abstract::The heart has epicardial adipose tissue that produces adipokines and mesenchymal stem cells. Systemic adipose tissue is involved in the pathophysiology of obesity-related heart diseases. However, the method for analyzing the direct interaction between adipose tissue and cardiomyocytes has not been established. Here we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1106
更新日期:2011-04-01 00:00:00
abstract::Prostaglandin E2 (PGE2) is specifically and reversibly bound by particulate fractions from adrenals from fetal calves and lambs. These binding sites appear to be located primarily in the adrenal medulla and have properties very similar to those which we have detected in adult human, bovine, and ovine adrenal medulla. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-6-2124
更新日期:1981-12-01 00:00:00
abstract::The effect of an activator of protein kinase C, 12-O-tetradecanoylphorbol-13-acetate (TPA), on the 5'-deiodinase (5'D) activity was studied in rat astroglial cells cultured in chemically defined medium. TPA promoted a large increase in the type II 5'D activity, which was maximal 5-10 h after addition of TPA and then d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1277
更新日期:1989-09-01 00:00:00
abstract::The secretion of LH, PRL, and cortisol was investigated in 4 sexually mature female rhesus macaques with cardiac catheters protected by tethers. Based on endocrine parameters, all 4 of the animals ovulated within 2 months from the time they were tethered, and regular menstrual cycles of 24-34 days were observed. The c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-261
更新日期:1984-07-01 00:00:00
abstract::Starvation depresses thyroid gland function. In addition, the peripheral turnover of thyroxine (T4) is reduced, in part due to decreased fecal elimination of T4. The present studies were performed to determine if starvation also affects the deiodinative pathway for T4 degradation. Rats were isotopically equilibrated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-6-1525
更新日期:1975-06-01 00:00:00
abstract::The epidermis has a great potential as a bioreactor to produce proteins with systemic action. However, the consequences of ectopic epidermal protein overexpression need to be carefully addressed to avoid both local and systemic adverse effects. Thus, the long-term effects of leptin on skin physiology have not been stu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0156
更新日期:2005-10-01 00:00:00
abstract::Ghrelin is an acylated peptide recently isolated from rat stomach that potently stimulates GH release in vitro and in vivo in rat and man. Ghrelin specifically activates the receptor for the growth hormone secretagogues (GHS-Rla), and it has been proposed as the endogenous ligand mimicked by these synthetic compounds....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.5.8894
更新日期:2002-05-01 00:00:00
abstract::The perinatal rat exhibits low serum TSH levels in the presence of very low concentrations of T3 and T4. We had reported previously that this animal does not require endogenous TRH for TSH release. We investigated here whether the inhibitory hormone somatostatin (SS) has a role in maintaining the low TSH secretory set...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1683
更新日期:1985-10-01 00:00:00
abstract::Task3 K(+) channels are highly expressed in the adrenal cortex and contribute to the angiotensin II and K(+) sensitivity of aldosterone-producing glomerulosa cells. Adult Task3(-/-) mice display a partially autonomous aldosterone secretion, subclinical hyperaldosteronism, and salt-sensitive hypertension. Here, we inve...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1101
更新日期:2013-08-01 00:00:00
abstract::Thyroid hormones are of considerable importance for vertebrate reproductive function and during development. To further assess the role of these compounds in this capacity, we examined the expression pattern of the type 2 iodothyronine deiodinase (D2), which converts T(4) to the more active hormone T(3), in the rat ut...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.5.8169
更新日期:2001-05-01 00:00:00
abstract::The ability of cells to directly respond to glucocorticoids and aldosterone is a function of GR and MR expression, and coexpression of 11beta-hydroxysteroid dehydrogenases (11betaHSDs), which convert glucocorticoids and their 11-ketometabolites into either receptor inactive or active derivatives. The aim of the presen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.1.8583
更新日期:2002-01-01 00:00:00
abstract::We investigated the effect of glucocorticoid on the generation of osteoclasts. In mouse bone marrow culture systems, dexamethasone, a synthetic glucocorticoid analog, enhanced osteoclast-like cell formation induced by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in a dose-dependent manner. Conversely, dexamethasone inhibit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.8119150
更新日期:1994-03-01 00:00:00
abstract::We developed a coculture system comprising primary rat adipocytes and L6 rat skeletal muscle cells to allow investigation of the effects of physiologically relevant mixtures of adipokines. We observed that coculture, or adipocyte-conditioned media, increased glucose uptake in muscle cells. An adipokine that could pote...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0020
更新日期:2007-09-01 00:00:00
abstract::Nitric oxide is a ubiquitous estrogen-regulated signaling molecule that has been implicated in the regulation of bone maturation and remodeling. To better understand the role that bone-cell-secreted nitric oxide plays in ovariectomy-induced modifications of bone turnover, we examined the expression of endothelial NO s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0334
更新日期:2006-09-01 00:00:00