Abstract:
:The nuclear orphan receptor human estrogen receptor-related receptor (ERR)-alpha is implicated in bone metabolism. We studied the effect of ERRalpha silencing in human mesenchymal stem cells (hMSCs) during osteoblastogenesis. We found that ERRalpha silencing led to an increase of bone sialoprotein and a decrease of osteopontin mRNA levels, suggesting enhanced osteoblastic differentiation. This was confirmed by an increased ability of hMSCs to deposit calcium. Concomitantly, knockdown of ERRalpha inhibited adipogenesis, resulting in a decrease in adipocyte number and adipocyte marker gene expression. In line with a negative role of ERRalpha in bone metabolism, we found that adult female and male ERRalpha-deficient mice displayed a moderate increase in femoral cancellous bone volume and density. Osteoblast surface was increased and marrow fat volume decreased in these animals. Furthermore, ERRalpha-deficient osteoblasts displayed increased differentiation properties in vitro in line with our observations in hMSCs. In summary, we identified a role for ERRalpha in bone mass regulation by affecting osteoblastic differentiation.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Delhon I,Gutzwiller S,Morvan F,Rangwala S,Wyder L,Evans G,Studer A,Kneissel M,Fournier Bdoi
10.1210/en.2009-0121subject
Has Abstractpub_date
2009-10-01 00:00:00pages
4463-72issue
10eissn
0013-7227issn
1945-7170pii
en.2009-0121journal_volume
150pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Animals maintained on retinol (vitamin A-alcohol)-deficient diets exhibit testicular atrophy and loss of the germinal epithelium. Retinoic acid (vitamin A-acid), when fed to retinol-deficient animals, does not prevent these lesions and had thus been thought not to play a role in the tests. Serum testosterone (T) level...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-6-2120
更新日期:1981-06-01 00:00:00
abstract::Immunological and binding methods have been used to demonstrate that acute inflammation induced in the pregnant mouse by a single sc turpentine injection elicits plasma protein responses in the fetal as well as in the maternal compartment. The maternal response involves, along with the classical pattern of positive an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-1782
更新日期:1987-05-01 00:00:00
abstract::Estrogen receptor alpha and beta proteins (ERalpha and ERbeta) at various stages of development of the rat mammary gland were quantified by Western blotting. ERalpha and ERbeta recombinant proteins were used as standards, and their molar concentrations were measured by ligand binding assays. In 3-week-old pregnant, la...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8260
更新日期:2001-07-01 00:00:00
abstract::To assess the consequences of prolonged exposure to elevated levels of LH/human chorionic gonadotropin (hCG) in the female, we developed a transgenic (TG) mouse model (hCGbeta+) that overexpresses the hCGbeta-subunit cDNA. Because of the promoter used, ubiquitin C, the transgene is expressed in multiple tissues, inclu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220490
更新日期:2002-10-01 00:00:00
abstract::This study analyzes the role of the autonomic nervous system, the pituitary gland, ACTH, dexamethasone (DEX), and thyroid hormones in the regulation of 5'deiodinase (5'D) in the hypothalamus (HP) and adrenal gland (AG) of the rat. 5'D activity was analyzed in rats under basal conditions (22 C) and during cold exposure...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628370
更新日期:1995-08-01 00:00:00
abstract::In the prostate, testosterone action depends on conversion to bioactive metabolites dihydrotestosterone and 17beta-estradiol (E2) via the 5alpha-reductase and aromatase enzymes, respectively. Exogenous estrogen inhibits prostate growth by indirect effects caused by suppression of pituitary gonadotropins and testicular...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220493
更新日期:2002-12-01 00:00:00
abstract::Diagnostic tests of hypothalamic-pituitary-adrenocortical function in psychiatric illness largely report the interaction of hypothalamic secretagogues with glucocorticoids at the pituitary level. This study investigated whether the efficiency of glucocorticoid inhibition is subject to modulation by intracellular proce...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220489
更新日期:2002-10-01 00:00:00
abstract::The potential role of basic fibroblast growth factor (bFGF) as a mediator of cell-cell interactions in the growth and development of the testis was examined. Nuclease protection analysis was used to evaluate bFGF gene expression in the testis and other male reproductive tract tissues. bFGF expression was evident in se...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1446630
更新日期:1992-12-01 00:00:00
abstract::We examined the effects of bombesin on rat pancreatic digestive enzyme gene expression using cloned complementary DNA probes for amylase, trypsinogen I, chymotrypsinogen B, and lysophospholipase. Rats were injected sc three times daily with 5 nmol/kg body wt bombesin. Pancreata were investigated after 6, 12, 24, 48, a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.3.1371450
更新日期:1992-03-01 00:00:00
abstract::Extensive remodeling of the follicular extracellular matrix occurs during the process of ovulation. This remodeling involves the breakdown of collagen, which is regulated, in part, by the action of the metalloproteinase collagenase and its associated inhibitors. In the present study, follicular metalloproteinase inhib...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-1-63
更新日期:1990-07-01 00:00:00
abstract::Although GH plays a key role in postnatal growth, prenatal growth is thought to be GH independent. However, recent data has shown GH receptor/binding protein (GHR/BP) to be present in rat fetal tissues as early as fetal stage E12. The aim of the present study was to investigate tissue-specific production of the GHR/BP...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.10.7664680
更新日期:1995-10-01 00:00:00
abstract::The metabolism of cholesterol sulfate (5-cholesten-3 beta-ol sodium sulfate) and cholesterol to pregnenolone sulfate and pregnenolone, respectively, in human fetal adrenal mitochondria has been investigated. Cholesterol sulfate (50 microM) was converted efficiently to pregnenolone sulfate (5-pregnen-3 beta-ol-20-one s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-1-208
更新日期:1982-07-01 00:00:00
abstract::Flavonoids are functional constituents of many fruits and vegetables. Some flavonoids have antidiabetic properties because they improve altered glucose and oxidative metabolisms of diabetic states. Procyanidins are flavonoids with an oligomeric structure, and it has been shown that they can improve the pathological ox...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0764
更新日期:2004-11-01 00:00:00
abstract::The roles of steroids in zebrafish sex differentiation, gonadal development, and function of the adult gonad are poorly understood. Herein, we used ferredoxin 1b (fdx1b) mutant zebrafish to explore such processes. Fdx1b is an essential electron-providing cofactor to mitochondrial steroidogenic enzymes, which are cruci...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2019-00068
更新日期:2019-10-01 00:00:00
abstract::DNA damage occurs as a result of environmental insults and aging and, if unrepaired, may lead to chromosomal instability and tumorigenesis. Because GH suppresses ataxia-telangiectasia mutated kinase phosphorylation, decreases DNA repair, and increases DNA damage accumulation, we elucidated whether GH effects on DNA da...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2019-00132
更新日期:2019-06-01 00:00:00
abstract::We have previously established the value of 2-dimensional electrophoretic mRNA activity profiles for investigating the hepatic genomic response to several metabolic perturbations, such as thyroid hormone or GH treatment, diabetes, high carbohydrate diet, starvation, and uremia. We now report the effects of adrenalecto...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2669
更新日期:1985-06-01 00:00:00
abstract::The GnRH receptor (GnRH-R) belongs to the rhodopsin/beta-adrenergic family of G protein-coupled receptors. The intracellular domains of these receptors, particularly the regions closest to the plasma membrane in intracellular loops 2 (2i) and 3 (3i) as well as some regions located in the membrane-proximal end of the C...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.5.6022
更新日期:1998-05-01 00:00:00
abstract::Variation in the onset of puberty among inbred strains of mice suggests that quantitative trait loci (QTLs) affect neurological and hormonal aspects of sexual maturation. Taking a novel approach toward identifying factors that regulate the hypothalamic-pituitary-gonadal (HPG) axis, we evaluated pubertal timing [as ass...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0543
更新日期:2004-10-01 00:00:00
abstract::The antiestrogen ICI 164,384 (ICI) binds the estrogen receptor (ER) with approximately 20% the affinity of estradiol, but without the partial agonistic effects caused by tamoxifen. Investigations into the mechanism of ICI action have used ER molecules expressed in vitro to examine the binding of ER to ICI and the capa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-2000
更新日期:1991-10-01 00:00:00
abstract::FSH and IGF-I are both important determinants of testicular development and Sertoli cell function. The present studies were performed to determine the actions of FSH and IGF-I on PI3K/AKT protein kinase signaling in immature rat Sertoli cells. Primary cultures of rat Sertoli cells were prepared from 10-d-old rats. Aft...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.6.8838
更新日期:2002-06-01 00:00:00
abstract::Mature 31- and 34-kDa inhibin A and B negatively regulate the release of FSH from the anterior pituitary; however, a direct comparison of these hormones in vivo has not been undertaken. The bioactivities of highly purified preparations of recombinant human 31-kDa inhibin A and B were determined in rat pituitary cells ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1783
更新日期:2009-10-01 00:00:00
abstract::Stimulation of rat granulosa cell aromatase activity by FSH has recently been used as a sensitive biological end point to develop an in vitro FSH bioassay. The present report provides a detailed validation and application of this assay. In the presence of androstenedione and diethylstilbestrol, FSH stimulated estrogen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1570
更新日期:1986-10-01 00:00:00
abstract::Corticosteroids stimulate and insulin inhibits energy acquisition (food intake); conversely, corticosteroids inhibit and insulin stimulates energy storage (body weight gain). Thus, together these hormones mediate long-term energy balance. This study tested whether the stimulatory action of corticosteroids on food inta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.5.7720670
更新日期:1995-05-01 00:00:00
abstract::Posttranslational processing of proadrenomedullin generates two biologically active peptides, adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP). Sequence comparison of homologous proadrenomedullin genes in vertebrate evolution shows a high degree of stability in the reading frame for AM, whereas P...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0105
更新日期:2006-07-01 00:00:00
abstract::The brain has traditionally been considered to be a target site of peripheral steroid hormones. By contrast, new findings over the past decade have shown that the brain itself also has the capability of forming steroids de novo, the so-called neurosteroids. De novo neurosteroidogenesis in the brain from cholesterol is...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2007-1592
更新日期:2008-06-01 00:00:00
abstract::Patients with noninsulin-dependent diabetes mellitus exhibit increased proportions of plasma proinsulin and proinsulin conversion intermediates. We used hyperinsulinemic diabetic and nondiabetic Psammomys obesus to study the possible relationship between steady state pancreatic insulin stores and the proportion of pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.2.8033810
更新日期:1994-08-01 00:00:00
abstract::Expression of annexin-I was investigated in the rat mammary gland during pregnancy, lactation, and involution. Both the mRNA and protein were very abundant in the mature virgin gland, but declined significantly by midpregnancy. In the lactating gland, little or no annexin-I was detected. After weaning, mRNA and protei...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1574
更新日期:1991-03-01 00:00:00
abstract::Recently, California condors (Gymnogyps californianus) have been reintroduced to coastal regions of California where they feed on marine mammal carcasses. There is evidence that coastal-dwelling condors experience reproductive issues, such as eggshell thinning, likely resulting from exposure to endocrine-disrupting ch...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1448
更新日期:2015-12-01 00:00:00
abstract::Recent evidence suggests that the aromatization of testosterone to estrogen is important for the organizing effects of neonatal testosterone on neuroendocrine responses to acute challenges. However, the extent to which neonatal inhibition of aromatase alters the stress-induced activation of neural pathways has not bee...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1749
更新日期:2012-04-01 00:00:00
abstract::Although estrogens and estrogen receptors (ERs) are known to function in the male brain and reproductive tract, few studies have evaluated their involvement in the male hematopoietic and immune systems. This study was undertaken to determine the role of ERalpha in hematopoietic progenitor and B lymphocyte maturation. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.7.7560
更新日期:2000-07-01 00:00:00