Abstract:
:The aim of this study was to assess the performance of a commercially available procedure for detecting anti-Sjögren's syndrome A (anti-SSA) and anti-Sjögren's syndrome B (anti-SSB) antibodies by immunoblotting (IB) and compare it with double immunodiffusion (DID). We also studied the clinical significance of these profiles in a series of unselected anti-SSA positive patients. Serum samples from 534 patients that were positive on an immunofluorescent screening test using HEp-2 cells were analysed for anti-SSA and anti-SSB antibodies by DID and IB (Biolab Anablot System II), and the results on anti-SSA antibodies were confirmed by an enzyme-linked immunosorbent assay (ELISA). Fifty-five serum samples were found to be positive for anti-SSA antibodies. Among these, 24 were anti-SSA negative by IB but positive by DID and ELISA ('non-blotter sera'), whereas only three serum samples were anti-SSA negative by DID but positive by IB and ELISA. Of the 18 anti-SSB positive serum samples, eight were negative by DID. All the serum samples that were anti-SSB positive by DID were also positive by IB. Anti-SSB antibodies showed a significant association with eye dryness and leucopenia. Anti-52 kDa SSA antibodies were associated with anti-SSB antibodies but showed no significant association with sicca symptoms, while anti-60 kDa SSA antibodies were associated with lower rates of leucopenia. The 'non-blotter' profile showed no significant association with any clinical parameter. IB is less sensitive than DID for detecting anti-SSA antibodies but more sensitive than DID for detecting anti-SSB antibodies. The determination of anti-SSA immunoblotting profiles in patients positive for anti-SSA antibodies by DID does not significantly improve the clinical usefulness of this test. As expected, anti-SSB antibodies were associated with clinical features of Sjögren's disease. Non-blotting (probably conformational) anti-SSA antibodies did not show any further association with clinical parameters and seem to have no clinical relevance.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Yago M,Belmonte MA,Olmos MJ,Beltran J,Teruel C,Segarra Mdoi
10.1177/000456329903600310subject
Has Abstractpub_date
1999-05-01 00:00:00pages
365-71eissn
0004-5632issn
1758-1001journal_volume
36 ( Pt 3)pub_type
杂志文章abstract::This study was undertaken to investigate the extent of the reported discrepancy between serum and plasma albumin concentrations measured by the bromocresol purple (BCP) method. We have shown that plasma albumin concentrations are falsely increased by turbidity due to the precipitation of fibrinogen when plasma is dilu...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328302000503
更新日期:1983-09-01 00:00:00
abstract::17-Hydroxyprogesterone (17-OHP) is an intermediate steroid in the adrenal biosynthetic pathway from cholesterol to cortisol and is the substrate for steroid 21-hydroxylase. An inherited deficiency of 21-hydroxylase leads to greatly increased serum concentrations of 17-OHP, while the absence of cortisol synthesis cause...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214529748
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abstract::591 patients with a history of coronary heart disease had one or more biochemical markers of tobacco smoking measured. 26% were self reported smokers and a further 4% were apparent 'smoking deceivers'. The urinary nicotine metabolite concentration is an excellent marker for tobacco smoking; breath CO would be a suitab...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329503200213
更新日期:1995-03-01 00:00:00
abstract::Proteinuria is the cardinal sign of renal disease, therefore accurate identification of clinically significant proteinuria is essential to the diagnosis and management of kidney disease. Spot samples are now widely used, namely protein: creatinine ratio (uPCR) and albumin: creatinine ratio (uACR). In this article we r...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/0004563212473283
更新日期:2013-07-01 00:00:00
abstract::Auto-antibodies to oxidized low-density lipoprotein (ox-LDL) are thought to play a pivotal role in the pathogenesis of atherosclerosis. This study investigates the value of auto-antibodies to ox-LDL as a predictive marker of atherosclerosis in patients with both homozygous and heterozygous familial hypercholesterolaem...
journal_title:Annals of clinical biochemistry
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abstract:BACKGROUND:As lifespan in HIV infection increases, cardiovascular disease has emerged as a cause of morbidity and mortality. Asymmetric dimethylarginine is an established marker of endothelial dysfunction and predicts cardiovascular events. The role of asymmetric dimethylarginine in HIV-related cardiovascular disease h...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563213505848
更新日期:2014-09-01 00:00:00
abstract:BACKGROUND:The rapid exclusion of acute myocardial infarction in patients with chest pain can reduce the length of hospital admission, prevent unnecessary diagnostic work-up and reduce the burden on our health-care systems. The combined use of biomarkers that are associated with different pathophysiological aspects of ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215578189
更新日期:2015-09-01 00:00:00
abstract::The seasonal variation of serum 25-hydroxy vitamin D3 and 1,25-dihydroxy-vitamin D has been investigated. Blood was taken from 27 healthy volunteers, aged 21-44 years old at 3 monthly intervals over a period of 1 year. A scrolling monthly programme with 12 quarterly (3 month) time periods was developed. A summer assoc...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329403100105
更新日期:1994-01-01 00:00:00
abstract::An investigation into the use of bilirubin oxidase to measure the apparent concentration of unbound bilirubin in solutions of bilirubin or bilirubin/human serum albumin demonstrated (1) near linearity of oxidation rate with bilirubin concentration up to 16 mumol/L, (2) linearity with respect to enzyme concentration up...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500110
更新日期:1988-01-01 00:00:00
abstract::Clinical biochemists have long known the analytical and clinical limitations of creatinine and creatinine clearance measurement in the assessment of glomerular filtration rate (GFR). This background is reviewed in the article before assessing the utility of cystatin C, the most promising replacement biochemical marker...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
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更新日期:2002-03-01 00:00:00
abstract::Adequate control of performance in immunoassays of maternal serum alpha-fetoprotein is an essential part of screening for neural tube defects. Information derived from external quality assessment schemes may be of limited value in monitoring individual laboratory performance and stresses the need for proper internal q...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328001700611
更新日期:1980-11-01 00:00:00
abstract::The ischaemic lactate-ammonia test is widely used for investigating patients with muscle pain and fatigue. It involves measuring plasma lactate and ammonia produced as a result of forearm exercise under ischaemic conditions in a fasted subject. Its clinical use is to screen patients with muscle complaints for disorder...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/0004563011900786
更新日期:2001-07-01 00:00:00
abstract::Circulating insulin-like growth factor-1 (IGF-1) is increasingly being used as a screening test and in ongoing monitoring of treated acromegaly. We here present three cases of women (two of whom were on the oestrogen containing contraceptive pill at the time of presentation) who had normal circulating IGF-1 and no ove...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307779596075
更新日期:2007-01-01 00:00:00
abstract::The deproteinised sample (150 microliters) is 'injected' into a continuously flowing stream of deionised water which is pumped, via a capillary tube, to the nebuliser of an atomic absorption spectrophotometer. Analytical readout is obtained, in the form of transient peaks, 6 s after sample injection. Before injection ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328302000202
更新日期:1983-03-01 00:00:00
abstract::Addison's disease is a relatively common disorder to endocrinologists, but is rare and potentially fatal when presenting acutely. Treatment now involves replacement of glucocorticoids and mineralocorticoids with synthetic compounds, although historically patients took common salt and plant-based preparations. We descr...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307780945624
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:Patient-based real-time quality control (PBRTQC) techniques have been described in clinical chemistry for over 50 years. PBRTQC has a number of advantages over traditional quality control including commutability, cost and the opportunity for real-time monitoring. However, there are few systematic investigati...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563220902174
更新日期:2020-05-01 00:00:00
abstract::The hypoglycaemia caused by non-islet cell tumours is often associated with an increase in plasma insulin-like activity. In many cases there is a relative if not always absolute increase in plasma insulin-like growth factor II (IGF-II). Growth hormone (GH) secretion is almost invariably depressed as is the plasma insu...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900312
更新日期:1992-05-01 00:00:00
abstract:BACKGROUND:To investigate the reasons why general practitioners (GPs) request rheumatoid factor (RF) assays, we studied 200 consecutive requests for RF from general practice in 1995. METHOD:By means of an audit questionnaire, we studied 100 negative, 50 positive and 50 borderline RF results and compared these with the...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303763046049
更新日期:2003-03-01 00:00:00
abstract::Most thyroid-stimulating hormone (TSH) assays now have the sensitivity required by thyroid guidelines and allow the reliable identification of patients with both overt and subclinical hyperthyroidism. Clinical guidelines usually quote decision limits for TSH, but often ignore the issue of whether variability in bias b...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307780480945
更新日期:2007-05-01 00:00:00
abstract:BACKGROUND:LC-MS/MS is rapidly becoming the technology of choice for measuring steroid hormones. We have developed a rapid LC-MS/MS assay for the routine analysis of serum cortisol. We have used this assay to investigate the effects of gender and exogenous steroid interference on the immunoassay measurement of serum co...
journal_title:Annals of clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1177/0004563212473448
更新日期:2013-07-01 00:00:00
abstract::We developed a new simple assay for potassium ion in serum using urea amidolyase (UAL) from yeast sp. The method is based on activation of the enzyme by potassium ion. We eliminated endogenous ammonium ion by use of glutamate dehydrogenase (GLDH), and then monitored the production of ammonium ion by UAL, urea, ATP, bi...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329703400408
更新日期:1997-07-01 00:00:00
abstract:BACKGROUND:Ascorbic acid can interfere with methodologies involving redox reactions, while comprehensive studies on main chemistry analysers have not been reported. We therefore attempted to determine the interference of ascorbic acid with analytes on the Beckman Synchron LX20. METHODS:Various concentrations of ascorb...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456305774538274
更新日期:2005-11-01 00:00:00
abstract::Background After adrenalectomy, urinary fractionated metanephrine concentrations are expected to be reduced. However, there are few studies suggesting cut-offs for adrenalectomy patients. Methods Urinary metanephrine and normetanephrine concentrations in adrenalectomy patients and two controls were compared and hormon...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215620822
更新日期:2017-01-01 00:00:00
abstract::A binding assay for sex hormone binding globulin (SHBG) has been developed in which SHBG is saturated with tritiated dihydrotestosterone (DHT). Separation of bound and free DHT is achieved by using Concanavalin A-Sepharose as a solid phase matrix. The method is described and its performance, including linearity, impre...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900208
更新日期:1992-03-01 00:00:00
abstract::The effect on the concentrations of specific serum proteins and tumour markers, of heat-treating samples at 56 degrees C for 30 min to inactivate HIV, was investigated. Statistically significant decreases, that may affect clinical decisions, were observed for alpha-1-antitrypsin, beta-2-microglobulin, IgE, fibrinogen,...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329002700611
更新日期:1990-11-01 00:00:00
abstract::Quadriceps muscle specimens from autopsy of 28 neonates (gestational age 25-42 weeks) were investigated to determine pyruvate and malate oxidation rates and several enzymes of the mitochondrial oxidative process. In general, the levels of all mitochondrial parameters measured, including carnitine levels, were lower in...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900604
更新日期:1992-11-01 00:00:00
abstract::The effect of ambient temperature on the analytical and clinical performance of a glucose meter was examined. A total of 114 venous whole blood samples were analysed for glucose by a reference method, and by a glucose meter at 21-22 degrees C, room temperatures, 26-27 degrees C and 33-34 degrees C. Glucose meter readi...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563011901091
更新日期:2001-11-01 00:00:00
abstract::The purpose of this study was to assess retrospectively the prevalence of severe hyponatraemia in a hospital population and its laboratory investigation, treatment and clinical outcome. Over a 6-month period 47 patients (27 women and 20 men) were found to have a plasma sodium concentration of < or = 120 mmol/L (this n...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329903600204
更新日期:1999-03-01 00:00:00
abstract:PURPOSE:We assessed erythrocyte sedimentation rate and high-sensitivity C-reactive protein concentration in knee osteoarthritis and non-knee osteoarthritis. In addition, we investigated potential relationship between the levels of erythrocyte sedimentation rate and high-sensitivity C-reactive protein with clinical find...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215610142
更新日期:2016-09-01 00:00:00
abstract::We hereby describe the rare case of a 59-year-old patient presenting with marked hyperamylasaemia mimicking acute pancreatitis upon admission. Investigation of co-existent hypokalemia revealed the presence of ectopic adrenocorticotropic hormone secretion, leading to the final diagnosis of small cell lung cancer, exhib...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563213500658
更新日期:2014-01-01 00:00:00