Abstract:
PURPOSE:Previous studies on the use of human amniotic membrane (HAM) in rabbit stem cell deficiency models have found the new epithelium growing over the HAM to express cornea-specific keratins (K3 and K12) in 40% of the cases, suggesting that HAM may have induced conjunctival epithelial cells to transdifferentiate into cornea-type epithelial cells. The current study was performed to determine whether HAM could induce transdifferentiation of conjunctival epithelia] cells when cultured in vitro. METHODS:Conjunctival grafts taken from the fornices of New Zealand white rabbits (6-12 weeks old) were placed over HAMs and lifted to an air-media interface using polypropylene double rings. These cultures were maintained in supplemented hormonal epithelial medium with and without 3T3 feeder cells. Rabbit corneal epithelial cells were cultured similarly using strips of keratolimbal grafts placed over HAM. The cultures were terminated at various times between the 8th and 15th day. The cultured epithelial cells were examined histologically and immunohistochemically using monoclonal antibodies AK-2 (to K12 keratin), AM-3 (to goblet cell mucin), and AE-5 (to K3 keratin). RESULTS:Both conjunctival and corneal epithelial cells cultured on HAMs showed multilayered, differentiated epithelial structures. On immunohistochemical examination, both epithelial cells stained positive for AE-5. None of the cultured conjunctival epithelial cells stained positively for AK-2, while the corneal epithelial cells showed positive staining with AK-2. There were no AM-3-positive goblet cells in either epithelial cell culture. There was no difference in the immunohistochemical patterns between cultures with or without 3T3 feeder cells. However, culture without feeder cells seemed to manifest a more degenerative appearance than those with feeders. CONCLUSION:HAM does not induce transdifferentiation of conjunctival epithelial cells into corneal-type epithelial cells under the in vitro culture conditions used in this study.
journal_name
Corneajournal_title
Corneaauthors
Cho BJ,Djalilian AR,Obritsch WF,Matteson DM,Chan CC,Holland EJdoi
10.1097/00003226-199903000-00013subject
Has Abstractpub_date
1999-03-01 00:00:00pages
216-24issue
2eissn
0277-3740issn
1536-4798pii
00003226-199903000-00013journal_volume
18pub_type
杂志文章相关文献
CORNEA文献大全abstract::A randomized, double-blind, placebo-controlled, multicenter clinical trial was conducted in five centers to assess safety, ocular tolerance, and efficacy of an ophthalmic solution of epidermal growth factor (EGF) for the treatment of traumatic corneal epithelial defects. One hundred four patients completed the study: ...
journal_title:Cornea
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1097/00003226-199207000-00007
更新日期:1992-07-01 00:00:00
abstract:PURPOSE:To evaluate the treatment with topical 1% cyclosporine A (CsA) in patients with subepithelial corneal infiltrates (SEIs). METHODS:We retrospectively reviewed the records of 9 patients (12 eyes) before and after the treatment with CsA 1% eyedrops twice daily. All patients had been treated with topical corticost...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181c33034
更新日期:2010-06-01 00:00:00
abstract:PURPOSE:To investigate the surgical procedure and therapeutic efficacy of femtosecond (FS) laser-assisted keratolimbal allograft (KLAL) transplantation in the treatment of eyes with total limbal stem cell deficiency. METHODS:Ten eyes from 10 patients who underwent FS laser-assisted KLAL transplantation were enrolled. ...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002041
更新日期:2019-10-01 00:00:00
abstract:PURPOSE:To evaluate the association between keratoconus (KC) and immune disorders (IDs). METHODS:A retrospective observational case-control study of all the members in the Central District of Clalit Health Services in Israel who were diagnosed to have KC (years 2000-2007; n = 426) and 1704 age- and gender-matched cont...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181cb410b
更新日期:2010-11-01 00:00:00
abstract:PURPOSE:This study was designed to report a case of biopsy-proven mucous membrane pemphigoid with severe bronchial involvement in a young woman. METHODS:Case report of a 17-year-old girl who presented with worsening dyspnea, skin rash, and bilateral ocular injection, symblepharon, and fornix foreshortening. Conjunctiv...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/01.ico.0000178730.34564.c7
更新日期:2006-05-01 00:00:00
abstract:PURPOSE:To demonstrate the feasibility of Descemet membrane endothelial keratoplasty (DMEK) performed after previous penetrating keratoplasty (PK) failure and to describe primary outcomes. METHODS:Twenty-eight eyes of 28 patients who underwent DMEK after primary PK failure between January 2013 and November 2017 were i...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001834
更新日期:2019-03-01 00:00:00
abstract:PURPOSE:To compare the rebubbling rate and clinical outcomes of Descemet membrane endothelial keratoplasty (DMEK) with 5% sulfur hexafluoride (SF6) gas versus 100% air as a tamponade for graft attachment. METHODS:Retrospective, comparative, interventional case series including 368 consecutive pseudophakic eyes with Fu...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001299
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:To investigate the long-term clinical course of scleromalacia with calcified plaque as a complication of cosmetic conjunctivectomy with topical mitomycin C (MMC) application and to introduce a surgical method for this complication using calcified plaque removal and/or conjunctival flap surgery. METHODS:Eleven ...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e318277ac92
更新日期:2013-06-01 00:00:00
abstract:PURPOSE:To analyze the variations in tear quantity and quality after corneal collagen cross-linking (CXL) in patients with keratoconus. METHODS:This is a prospective observational study of 24 eyes with keratoconus that underwent epithelium-off CXL. The variables studied were tear film osmolarity, subjective symptoms (...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001085
更新日期:2017-02-01 00:00:00
abstract:PURPOSE:To compare the outcome of bifold forceps and cartridge injector suture pull-through insertion techniques for Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS:A retrospective review was conducted of the medical records of all patients treated with DSAEK at the University of Iowa Hospitals ...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181eadb84
更新日期:2011-03-01 00:00:00
abstract:PURPOSE:To assess the reproducibility of relatives' replies to questions asked at the time of corneal donation and again 2 months later concerning infectious disease risk factors and to identify characteristics associated with inconsistent responses. METHODS:A reinterview study involving individuals who answered quest...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-200211000-00014
更新日期:2002-11-01 00:00:00
abstract::Primary graft failure is a rare but major complication of penetrating keratoplasty. An apparent clustering of four cases during a 1-month period led to a review of all 778 keratoplasties performed by nine surgeons at Massachusetts Eye and Ear Infirmary from July 1, 1986 to June 30, 1988. The objectives of this study w...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-199407000-00005
更新日期:1994-07-01 00:00:00
abstract:PURPOSE:Posterior amorphous corneal dystrophy (PACD) is a rare disorder characterized by sheet-like opacification of the posterior corneal stroma, corneal thinning, and decreased corneal curvature. It is not known to be associated with progressive corneal ectasia. In this report, we examine the course of a patient with...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001304
更新日期:2017-12-01 00:00:00
abstract:PURPOSE:To report 2 cases of corneal perforation associated with a persistent epithelial defect (PED), which were treated with eye drops containing the fibronectin-derived peptide PHSRN (Pro-His-Ser-Arg-Asn). METHODS:A 67-year-old man and a 58-year-old man presented with corneal perforation associated with a PED cause...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000592
更新日期:2015-11-01 00:00:00
abstract:PURPOSE:To investigate agreement between rotating Scheimpflug camera (Pentacam HR, Oculus) and rotating Scheimpflug camera combined with Placido disc corneal topography (Sirius, CSO) in measuring total corneal astigmatism (TCA). METHODS:In this observational study, all patients undergoing cataract surgery with preoper...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001117
更新日期:2017-04-01 00:00:00
abstract:PURPOSE:To report our experience in the treatment of intraepithelial neoplasia of the conjunctiva using topical bevacizumab. METHODS:Ten eyes of 10 patients with conjunctival neoplasia received 25 mg/mL bevacizumab topically. Changes in the lesions were documented weekly using digital photography. After topical treatm...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000194
更新日期:2014-11-01 00:00:00
abstract:PURPOSE:To analyze tear meniscus dimensions with Fourier domain-optical coherence tomography (FD-OCT) in patients with type 2 diabetes mellitus. METHODS:Patients with type 2 diabetes without diabetic retinopathy (DR) (n = 54), with nonproliferative DR (n = 45), with proliferative DR (PDR) (n = 25), and controls (n = 4...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000651
更新日期:2015-12-01 00:00:00
abstract:PURPOSE:To describe a new technique for donor insertion in cases of Descemet-stripping automated endothelial keratoplasty (DSAEK). METHODS:This is a retrospective case series study of a novel method to introduce the donor tissue in DSAEK. This is a report of 16 cases that underwent the DSAEK procedure. The surgical te...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e31816f62bd
更新日期:2008-08-01 00:00:00
abstract:PURPOSE:The purpose of this case series was to evaluate the effectiveness of modified deep anterior lamellar keratoplasty in eyes with scarring secondary to hydrops associated with keratoconus. METHODS:Four patients underwent modified deep anterior lamellar keratoplasty. This was performed by controlled air injection ...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181ca2b8c
更新日期:2011-02-01 00:00:00
abstract:PURPOSE:To test the hypothesis that patients with keratoconus and pellucid who have glaucoma or are glaucoma suspects have lower corneal hysteresis (CH) and/or corneal resistance factor (CRF) measurements compared with controls. METHODS:A prospective study at a tertiary eye center of patients with keratoconus and pell...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181ca363c
更新日期:2010-09-01 00:00:00
abstract::Severe microbial keratitis developed in a 74-year-old women receiving long-term corticosteroids. Corneal smears revealed slender, fusiform gram-negative bacilli, but the lesion continued to worsen despite intensive antibiotic treatment. After several days, Capnocytophaga sputigena was identified on culture. Specific a...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract:PURPOSE:To evaluate the morphological features of the corneal limbus as measured by in vivo confocal microscopy (IVCM) following standard and accelerated corneal collagen cross-linking (CXL) for keratoconus. METHODS:Patients with progressive keratoconus scheduled to undergo standard CXL (group 1; 31 patients, 3 mW/cm,...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001029
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:To evaluate the efficacy, predictability, and safety of combined corneal collagen cross-linking (CXL), intracorneal ring segment (ICRS) implantation, and superficial phototherapeutic keratectomy (PTK) in patients with keratoconus. METHODS:Fifty-five eyes received ICRS implantation, followed by CXL and PTK comb...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002073
更新日期:2019-10-01 00:00:00
abstract:PURPOSE:To report the histopathologic findings in the host tissue of 2 human keratoconic corneas undergoing maximum-depth anterior lamellar keratoplasty (MDALK) using the manual dissection technique described by Melles. METHODS:Corneal buttons were examined from 2 patients with keratoconus who underwent MDALK using th...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/01.ico.0000179930.97651.8f
更新日期:2006-09-01 00:00:00
abstract:PURPOSE:To assess whether omega-3 fatty acid (FA) supplementation is more efficacious than placebo in amelioration of signs and symptoms of dry eye disease. METHODS:We performed a systematic literature search in PubMed, Scopus, Web of Science, and Cochrane Central Register of Controlled Trials databases. We included r...
journal_title:Cornea
pub_type: 杂志文章,meta分析
doi:10.1097/ICO.0000000000001884
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:This study was designed to report a case of acanthamoeba keratitis in a 5-year-old child without a history of trauma or contact lens usage. METHODS:The history, clinical presentation, diagnostic, and therapeutic approaches were reviewed. RESULTS:A 5-year-old child without any history of trauma or contact lens...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/01.ico.0000178723.26940.74
更新日期:2006-04-01 00:00:00
abstract:PURPOSE:To evaluate the effectiveness of intraoperative mitomycin C in preventing the recurrence of primary pterygium. METHODS:In this prospective, randomized study, 38 eyes of 35 patients with primary pterygium were evaluated. In 19 eyes, the "bare sclera technique" combined with intraoperative application of 0.2 mg/...
journal_title:Cornea
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00003226-200007000-00013
更新日期:2000-07-01 00:00:00
abstract:PURPOSE:To describe the association of water exposure with contact lens (CL)-related disease and explore the guidelines regarding water exposure to CL wearers, provided by CL manufacturing industry, global public health, and CL-related professional associations. METHODS:A review of the literature was conducted by sear...
journal_title:Cornea
pub_type: 杂志文章,评审
doi:10.1097/ICO.0000000000001898
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:To describe the management and outcome of an ocular surface alkali burn in the setting of previous laser in situ keratomileusis (LASIK). METHODS:This is a case report and review of relevant literature. RESULTS:A 25-year-old man with a history of LASIK presented 4 weeks after a sodium hydroxide splash to his l...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002604
更新日期:2020-12-03 00:00:00
abstract::Two patients developed graft versus host disease (GvHD) following allogeneic bone marrow transplantation for leukemia. One patient developed acute GvHD 12 days after transplantation, and the second developed chronic GvHD 100 days after transplantation. Tear analysis and conjunctival impression cytology were performed....
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00