Abstract:
:We investigated the effects of two sex steroids (17beta estradiol and testosterone) on five human leukemia cell lines. We observed a statistically significant inhibition of proliferation, dose and time dependent, of the human monoblastic leukemia cell line U937. This inhibition was associated with a dose dependent decrease in the number of CFU-blasts in clonogenic cultures. Cytostatic effect was obtained with doses of 5 microM for estrogen and 10 microM for androgen and was not due to a non-specific cytotoxic effect, some cell viability remained high (> 90%) even after 6 days of incubation. More accurately, we demonstrated that growth inhibition was associated with a cell cycle arrest, U937 cells accumulating in G2/M phase. This blockade was dose related with a maximum number of cells accumulating at day 4. Sensitivity of these cells to an S-phase specific agent (hydroxyurea) was not increased, suggesting that these cells were blocked in G2/M and did not undergo mitosis. Expression in U937 cells of high affinity nuclear receptors for estrogen and androgen was negative which was in favour of a type II estrogen binding site, mediated mechanism. Moreover, a small fraction of these cells underwent apoptosis or differentiation with about 12% apoptotic cells and a significant increase (more than 30%) of two myelomonocytic markers (CD13 and CD64). These results demonstrate that the proliferation of some leukemic cells may be inhibited by micromolar concentrations of sex steroids, independently of nuclear receptor expression. The main mechanism seems to be a block in cell cycle associated with modulation of apoptosis and differentiation. It provided additional evidence for the potential value of sex steroids and their analogues in the treatment of leukemias.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Mossuz P,Cousin F,Castinel A,Chauvet M,Sotto MF,Polack B,Sotto JJ,Kolodie Ldoi
10.1016/s0145-2126(98)00101-5subject
Has Abstractpub_date
1998-11-01 00:00:00pages
1063-72issue
11eissn
0145-2126issn
1873-5835pii
S0145-2126(98)00101-5journal_volume
22pub_type
杂志文章abstract::Internal tandem duplications (ITDs) of the FLT3 gene occur in approximately 20-30% of acute myeloid leukemia (AML) patients. We investigated if FLT3 ITDs could be used as minimal residual disease (MRD) markers for AML patients. Patient-specific polymerase chain reaction (PCR) assays for FLT3 ITDs were developed for fo...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00087-x
更新日期:2001-12-01 00:00:00
abstract::Adult T-cell leukemia/lymphoma is a distinct clinical entity characterized by a clonal proliferation of malignant T-lymphocytes. The etiologic agent of the disease is a Human T-cell lymphotropic virus type I. It occurs almost exclusively in areas where the virus is endemic; however the disease develops only in the min...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.02.011
更新日期:2010-12-01 00:00:00
abstract::We performed a systematic review and meta-analysis of all trials comparing MMF and methotrexate as GVHD prophylaxis. Our search yielded 11 studies; 3 were randomized-control trials (RCTs). While the incidence of grades 2-4 acute GVHD was comparable, the incidence of grades 3 and 4 acute GVHD was higher in patients giv...
journal_title:Leukemia research
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.leukres.2013.12.012
更新日期:2014-03-01 00:00:00
abstract::This study examines the occurrence, subtype and possible significance of haemopoietic inhibitory T cells (HIT cells) in patients with myelodysplasia (n = 21), acute myeloblastic leukaemia (n = 8) and in normal subjects (n = 13) using an autostimulary CFU-GM stem cell assay. HIT cells were detected when colony numbers ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(91)90028-r
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Most patients with myelodysplastic syndromes (MDS) require transfusions at the risk of iron overload and associated organ damage, and death. Emerging evidence indicates that iron chelation therapy (ICT) could reduce mortality and improve survival in transfusion-dependent MDS patients, especially those classi...
journal_title:Leukemia research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.leukres.2014.02.003
更新日期:2014-05-01 00:00:00
abstract::Cytogenetic analysis provides important diagnostic and prognostic information for patients with myelodysplastic syndromes (MDS). Prior studies, mostly comprised of small sample sizes, have reported conflicting results while evaluating the usefulness of FISH in addition to G-band karyotyping in MDS. In the current stud...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.09.013
更新日期:2010-04-01 00:00:00
abstract::Quantitative differences in HLe-1 expression were studied on normal lymphocytes and lymphoblasts of patients with acute lymphoblastic leukemia (ALL). A relationship was found between quantitative antigen expression and therapeutic outcome. Alterations in fluorescence intensity (FI) were demonstrated using quantitative...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90110-x
更新日期:1987-01-01 00:00:00
abstract::Many elderly patients with newly diagnosed acute myeloid leukemia (AML) present with cardiac comorbidity precluding the use of anthracycline containing chemotherapy regimens. Amsacrine, a topoisomerase II inhibitor, has been proposed as possible alternative to anthracyclines. Here, we report about the combination of a...
journal_title:Leukemia research
pub_type: 临床试验,杂志文章
doi:10.1016/j.leukres.2007.06.015
更新日期:2008-03-01 00:00:00
abstract::To examine the possibility that ETS family transcription factors, PU.1, SPI-B, ELF-1, ERG-3, ETS-1 and TEL, and homeodomain proteins, HOXA10, HOXC13, MEIS1 and PBX1B, function cooperatively, we investigated their interactions. In luciferase assays, HOXA10 and HOXC13 augmented the activity of PU.1 and SPI-B while dimin...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.07.002
更新日期:2009-03-01 00:00:00
abstract::1,25-Dihydroxyvitamin D3 (1,25D) induces differentiation of myeloid leukemia cells, but resistant cells are also encountered. We studied the mechanistic basis for the resistance in a model system using enhancers of 1,25D, the antioxidant carnosic acid and a kinase inhibitor SB202190. Knock-down (KD) of JNK2p54 unexpec...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.03.003
更新日期:2009-10-01 00:00:00
abstract::Most clinicians caring for patients with AML do not use the word "cure" casually, since for many patients diagnosed with AML, a state of cure or even of long term survival remains elusive. Analysis of prognostic factors may aid in defining the chance for cure in various AML subtypes, and improvements are required at a...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2012.08.006
更新日期:2012-12-01 00:00:00
abstract::Defective apoptosis is a hallmark of the progression of B chronic lymphocytic leukaemia (B-CLL). Smac-mimetics have been shown to induce apoptosis in several tumours. We describe the in vitro pro-apoptotic activity and regulation of the molecular pathway induced by new Smac-mimetics in B-CLL. The cytotoxic effect was ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.03.016
更新日期:2013-07-01 00:00:00
abstract::Platelet transfusion refractoriness (PTR) is a major adverse event in the management of acute myeloid leukemia (AML). In a series of 897 adult patients with AML receiving intensive chemotherapy, we identified 41 patients (4.8%) with PTR. PTR was more frequently observed in parous women, patients with extra-medullary d...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2017.08.015
更新日期:2017-10-01 00:00:00
abstract::The aberrant changes of tumor suppressor genes (TSGs) are now recognized as an important mechanism contributing to the development of acute myeloid leukemia (AML). CCAAT/enhancer binding protein zeta (C/EBPζ), a candidate TSG, has been found to be involved in cancers including AML. We detected the methylation status o...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.10.031
更新日期:2011-07-01 00:00:00
abstract::We report a patient with Philadelphia chromosome positive (Ph +ve) chronic myelogenous leukemia (CML), treated with hydroxyurea alone, who upon disease progression developed an additional Ph - ve clone containing chromosomal abnormalities typical of myelodysplastic syndrome (MDS). Retrospective analysis of a cryoprese...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00140-0
更新日期:2002-04-01 00:00:00
abstract::Chronic myeloid leukaemia (CML) patients in chronic phase (CP) are currently treated with a standard dose of imatinib of 400mg/daily. However, once in complete cytogenetic remission (CCR) it is possible that some patients maintain this status with reduced dose of the drug. Here, we describe five cases of CML in late C...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.10.024
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:T- cell prolymphocytic leukemia (T- PLL) is a rare aggressive hematological malignancy. Alemtuzumab, an anti-CD52 humanized monoclonal antibody, is the treatment of choice for remission induction. Allogeneic hematopoietic cell transplantation (allo-HCT) has been described to induce durable remissions and imp...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2018.01.009
更新日期:2018-04-01 00:00:00
abstract::Delta retrovirus-mediated leukemogenesis is dependent on the oncogenic potential of Tax. It is not clear, however, whether Tax-specific immune responses play a role in leukemia onset and progression. Using the BLV-associated leukemia model in sheep, we found that Tax-specific cytotoxic responses induced by DNA immuniz...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.06.003
更新日期:2010-12-01 00:00:00
abstract::The mechanism of the inhibition of proliferation of human leukaemia 60 (HL60) cells by S-D-lactoylglutathione in vitro was investigated. The median inhibitory concentration IC50 value was 66 microM (95% C.I. 50-87 microM; n = 18). The inhibition of leukaemia cell growth required exposure of HL60 cells to S-D-lactoylgl...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(93)90017-f
更新日期:1993-04-01 00:00:00
abstract::Hyaluronan (HA) modulates multidrug resistance (MDR) as well as cell migration. Tiam1 is involved in cytoskeleton reorganization during tumor invasion. In this report we show the relationship among HA, Tiam1, migration and MDR in murine lymphoma cell lines. We observed that MDR cells presented higher migratory capacit...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.02.020
更新日期:2010-11-01 00:00:00
abstract::This study examined the immunoregulatory role of recombinant interleukin 4 (IL-4), also known as B-cell stimulating factor 1, on the generation of cytotoxic effector cells from normal and leukaemic human blood mononuclear cells. When tested on cells from normal individuals, the addition of IL-4 to mixed lymphocyte cul...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90066-0
更新日期:1989-01-01 00:00:00
abstract::microRNA profiling of acute myeloid leukemia patient samples identified miR-125a as being decreased. Current literature has investigated miR-125a's role in normal hematopoiesis but not within acute myeloid leukemia. Analysis of the upstream region of miR-125a identified several CpG islands. Both precursor and mature m...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.12.021
更新日期:2014-03-01 00:00:00
abstract::In acute lymphoblastic leukemia (ALL) patients, automated fluorescence fragment analysis (ALF) has been reported to improve the monoclonality detection rate of immunoglobulin heavy chain genes (IgH) polymerase chain reaction (PCR) analysis. This study performed complementary determining region (CDR) I and III PCR on s...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00025-x
更新日期:2001-09-01 00:00:00
abstract::We have demonstrated that constitutive signal transducer and activator of transcription (STAT) 3 activity, observed in approximately 50% of acute myeloid leukemia (AML) cases, is associated with adverse treatment outcome. Constitutive STAT3 activation may result from the expression of oncogenic protein tyrosine kinase...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2007.11.035
更新日期:2008-07-01 00:00:00
abstract::Ten patients with acute nonlymphocytic leukemia (ANLL) received bromodeoxyuridine (BrdU) at 100 mg/M2 intravenously over 1 h. BrdU is incorporated into the DNA by S-phase cells and was detected by using a monoclonal anti-BrdU antibody in the bone marrow aspirate (BM) and biopsy specimens obtained at the end of the inf...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90160-3
更新日期:1987-01-01 00:00:00
abstract::One of the major limitations of DNA flow cytometry (FCM) in hematologic malignancies is the lack of information about the proliferation activity of subpopulations of the heterogeneous bone marrow (BM) compartment. We studied the S-phase DNA content of immunophenotypically defined BM subpopulations (CD2+; CD19+; CD2/CD...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(91)90467-8
更新日期:1991-01-01 00:00:00
abstract::We investigated the therapeutic activity of recombinant erythropoietin (r-EPO) in association with thalidomide in 30 patients with myelodysplastic syndromes (MDS), previously treated with r-EPO (n.15, group A) or thalidomide (n.15, group B) as single agents, respectively, without any significant benefit on their anemi...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.08.020
更新日期:2006-04-01 00:00:00
abstract::Fifty-three patients with Ph positive chronic myeloid leukaemia in blastic phase were studied. Additional abnormalities were found in 29 (55%) patients and were more common in myeloid (64%) than lymphoid (45%) blast crisis. The most frequent were +Ph (32%), +8 (28%), +19 (19%), +20 (9%) and +21 (9%). i(17q) (9%) was a...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(94)90023-x
更新日期:1994-05-01 00:00:00
abstract::An insertion (10;11)(p11;q23q24) was found in bone marrow metaphase cells from two children with acute monocytic leukemia (AMoL-M5b). This rearrangement involves a small chromosomal segment of 11q and may be misinterpreted as a deletion of 11q. Insertion (10;11) may represent a new recurring abnormality involving 11q ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(85)90140-7
更新日期:1985-01-01 00:00:00
abstract::We compared the growth-inhibitory effects and inhibition profile of the SRC family kinases (SFKs) of imatinib, dasatinib, nilotinib and INNO-406. Dasatinib exhibited the strongest potency against BCR-ABL with little selectivity over SFKs. Nilotinib exhibited a weaker affinity than the other inhibitors, but was highly ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.11.008
更新日期:2008-06-01 00:00:00