当前位置： SCI文献检索 > LEUKEMIA RESEARCH期刊下所有文献 > Quantitative, real-time polymerase chain reactions for FLT3 internal tandem duplications are highly sensitive and specific.

Quantitative, real-time polymerase chain reactions for FLT3 internal tandem duplications are highly sensitive and specific.

Abstract：

:Internal tandem duplications (ITDs) of the FLT3 gene occur in approximately 20-30% of acute myeloid leukemia (AML) patients. We investigated if FLT3 ITDs could be used as minimal residual disease (MRD) markers for AML patients. Patient-specific polymerase chain reaction (PCR) assays for FLT3 ITDs were developed for four AML samples that contained FLT3 ITDs of varying size and location. The real-time, quantitative PCR assays for FLT3 ITDs were highly sensitive and specific, detecting between 0.01 and 0.001% of FLT3 ITD positive DNA in a background of 1 microg of normal bone marrow DNA. Our findings suggest that FLT3 ITDs can be used as molecular markers for MRD in patients with AML.

journal_name

Leuk Res

journal_title

Leukemia research

authors

Stirewalt DL,Willman CL,Radich JP

doi

10.1016/s0145-2126(01)00087-x

keywords:

subject

Has Abstract

pub_date

2001-12-01 00:00:00

pages

1085-8

issue

12

eissn

0145-2126

issn

1873-5835

pii

S014521260100087X

journal_volume

25

pub_type

杂志文章

相关文献

LEUKEMIA RESEARCH文献大全
  • WITHDRAWN: The effect of dihydroartemisinin on TfR and VEGF expression in iron overload human myeloid leukemia K562 cells.

    abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2009.01.006

    authors： Zengwang,Zhou HJ,Li A,Zhang JL

    更新日期：2009-02-20 00:00:00

  • Screening for induction of differentiation and toxicity to blast cells by chemotherapeutic compounds in human myeloid leukemia.

    abstract：:Bone marrow cells from 9 patients with acute myeloid leukemia and 1 patient with a blast crisis of chronic myeloid leukemia were cultured to determine their ability to be induced to differentiate by different chemotherapeutic compounds. Five of these 10 patients showed differentiation to granulocytic and/or monocytic ...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/0145-2126(85)90087-6

    authors： Lotem J,Berrebi A,Sachs L

    更新日期：1985-01-01 00:00:00

  • Enhanced topoisomerase II-induced DNA breaks and free radical production by a new anthracycline with potent antileukemic activity.

    abstract：:In a previous study we reported that a new anthracycline derivative (moflomycin) exhibited a higher antileukemic activity compared to other anthracyclines, such as daunorubicin and doxorubicin. To explain the superior antileukemic effect of moflomycin and to disclose a possible structure-activity relationship, we inve...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/0145-2126(95)00155-7

    authors： Andrivon W,Saucier JM,Auclair C,Monneret C,Florent JC,Guillosson JJ,Nafziger J

    更新日期：1996-02-01 00:00:00

  • Preclinical and clinical results with pomalidomide in the treatment of relapsed/refractory multiple myeloma.

    abstract：:Despite the evolution of effective frontline treatment strategies, many patients with myeloma inevitably relapse. Treatment can be complicated by the interplay of disease-, treatment-, and patient-related factors. Unfortunately, many patients eventually develop disease that is refractory to lenalidomide and bortezomib...

    journal_title：Leukemia research

    pub_type： 杂志文章,评审

    doi：10.1016/j.leukres.2014.02.008

    authors： Mark TM,Coleman M,Niesvizky R

    更新日期：2014-05-01 00:00:00

  • Effects of X-irradiation, ovariohysterectomy and estradiol-17 beta on incidence, benign/malignant ratio and multiplicity of rat mammary neoplasms--a preliminary report.

    abstract：:An overview is given of the effects of X-irradiation, ovariohysterectomy and estradiol-17 beta administration on mammary tumorigenesis in females of 3 rat strains, viz. the WAG/Rij, BN/BiRij and SD. The 3 rat strains differed significantly in their spontaneous mammary tumor incidence. Female SD rats had the highest in...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/0145-2126(86)90292-4

    authors： Solleveld HA,van Zwieten MJ,Broerse JJ,Hollander CF

    更新日期：1986-01-01 00:00:00

  • An autologous tumor vaccine for CLL.

    abstract：:Chronic Lymphocytic Leukemia B cells (CLL) are malignant cells which retain at least some functions of normal B cells. Paramount amongst the latter is that when such cells are appropriately stimulated, they are able to present antigens, including any potential tumor antigens, making them excellent choices as a candida...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2018.03.002

    authors： Zhu F,Khatri I,Spaner D,Gorczynski RM

    更新日期：2018-05-01 00:00:00

  • Prototypical HTLV-I/II infection is rare in LGL leukemia.

    abstract：:The etiology of LGL leukemia is not known; however, we recently detected HTLV-II in a patient with LGL leukemia. In this study, we found that sera from 6 of 28 patients with LGL leukemia were positive for HTLV-I/II using a whole virus ELISA; moreover, the ELISA-negative sera were near the positive cut-off value. There...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/0145-2126(94)90078-7

    authors： Loughran TP Jr,Sherman MP,Ruscetti FW,Frey S,Coyle T,Montagna RA,Jones B,Starkebaum G,Poiesz BJ

    更新日期：1994-06-01 00:00:00

  • 2CdA chemotherapy and rituximab in the treatment of marginal zone lymphoma.

    abstract：:Standard chemotherapic approach for MZL is missing. We are presenting our monocenter experience with 2CdA+/-rituximab. Patients received 2CdA, 5mg/m(2), weekly, for 6 weeks. Patients receiving rituximab underwent to antibody administration in association with 2CdA, or after the end of chemotherapy. Global ORR was 89.3...

    journal_title：Leukemia research

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.leukres.2009.04.003

    authors： Orciuolo E,Buda G,Sordi E,Baraté C,Galimberti S,Ciancia E,Petrini M

    更新日期：2010-02-01 00:00:00

  • Evidence that knock down of GSK-3β in Chronic Myelogenous Leukemia cells augments IFN-γ-induced apoptosis.

    abstract：:The role of interferon-gamma (IFN-γ) in Chronic Myelogenous/Myeloid Leukemia (CML) and in the treatment of CML remains unclear; specifically, the effect of IFN-γ on apoptosis. There is reported interplay between IFN-γ and glycogen synthase kinase-3 (GSK-3), a kinase which has been implicated in both cell death and, co...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2020.106464

    authors： Kauffman MR,Nazemidashtarjandi S,Ghazanfari D,Allen AE,Reynolds NM,Faik A,Burdick MM,McCall KD,Goetz DJ

    更新日期：2020-12-01 00:00:00

  • In vitro cellular drug sensitivity at diagnosis is correlated to minimal residual disease at end of induction therapy in childhood acute lymphoblastic leukemia.

    abstract：:Leukemic cells from 85 children with newly diagnosed precursor B-lineage ALL were tested for in vitro drug sensitivity to a panel of anti-cancer drugs. Minimal residual disease (MRD) was measured by RQ-PCR. There was a significant correlation between MRD day 29 and in vitro sensitivity to prednisolone (p<0.001) and do...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2008.06.012

    authors： Lönnerholm G,Thörn I,Sundström C,Frost BM,Abrahamsson J,Behrendtz M,Heldrup J,Jacobsson S,Li A,Olofsson T,Porwit A,Söderhäll S,Larsson R,Forestier E

    更新日期：2009-01-01 00:00:00

  • Genotype of glutathione S-transferase and other genetic configurations in myelodysplasia.

    abstract：:We examined polymorphisms of glutathione S-transferase (GST) genes in 159 Japanese patients with myelodysplasia and compared the incidence with that in 43 normal individuals to clarify their pathogenetic significance in myelodysplasia. In individuals with the GSTT1 null genotype, the odds ratios for disease risk were ...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/s0145-2126(99)00119-8

    authors： Sasai Y,Horiike S,Misawa S,Kaneko H,Kobayashi M,Fujii H,Kashima K,Taniwaki M

    更新日期：1999-11-01 00:00:00

  • Comparison of imatinib, dasatinib, nilotinib and INNO-406 in imatinib-resistant cell lines.

    abstract：:We compared the growth-inhibitory effects and inhibition profile of the SRC family kinases (SFKs) of imatinib, dasatinib, nilotinib and INNO-406. Dasatinib exhibited the strongest potency against BCR-ABL with little selectivity over SFKs. Nilotinib exhibited a weaker affinity than the other inhibitors, but was highly ...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2007.11.008

    authors： Deguchi Y,Kimura S,Ashihara E,Niwa T,Hodohara K,Fujiyama Y,Maekawa T

    更新日期：2008-06-01 00:00:00

  • The sensitivity to methylnitrosourea-induced T-cell leukemogenesis in mice is modified by the injection of hydrocortisone.

    abstract：:T-cell leukemias were induced in adult BDF1 mice by a single i.v. injection of methylnitrosourea (MNU). Leukemogenesis was delayed by a single or repeated injections of hydrocortisone (HC) after MNU and also when HC was given one day before MNU. Enhancement of leukemogenesis was seen in experiments with 10 and 14 days...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/0145-2126(85)90143-2

    authors： Seidel HJ,Kreja L

    更新日期：1985-01-01 00:00:00

  • Monosomy 7 and Ph-positive acute lymphoblastic leukaemia: cytogenetic and molecular aspects.

    abstract：:A combination of monosomy 7 and translocation t(9;22) (q34;q11), rarely observed in acute lymphoblastic leukaemia (ALL), is here reported: a peculiarity of this case was that the "breakpoint cluster region" on chromosome 22 was not rearranged, as demonstrated by molecular analysis, and a new c-abl protein (p190) was f...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/0145-2126(87)90114-7

    authors： Eridani S,Gorman P,Sheer D,Duncombe AS,Glass UH,Shivji MK

    更新日期：1987-01-01 00:00:00

  • Imatinib has limited therapeutic activity for hypereosinophilic syndrome patients with unknown or negative PDGFRalpha mutation status.

    abstract：:Hypereosinophilic syndrome (HES) is characterized by sustained non-clonal blood and tissue eosinophilia, leading to end-organ damage. With a molecular/cytogenetic clonality marker, the disease is classified as chronic eosinophilic leukemia (CEL). Efficacy of imatinib mesylate is well established in CEL with FIP1L1-pla...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2008.10.004

    authors： Jain N,Cortes J,Quintás-Cardama A,Manshouri T,Luthra R,Garcia-Manero G,Kantarjian H,Verstovsek S

    更新日期：2009-06-01 00:00:00

  • MCP-1 in the cerebrospinal fluid of children with acute lymphoblastic leukemia.

    abstract：BACKGROUND:Monocyte chemoattractant protein-1 (MCP-1) is a member of the CC chemokines. MCP-1 has been previously shown to have a major role in the migration of monocytes towards human leukemic cells, yet it cannot increase cytotoxic effects of monocytes on human leukemic cells. AIM:To determine levels of MCP-1 in the...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2006.01.017

    authors： Eisenkraft A,Keidan I,Bielorai B,Keller N,Toren A,Paret G

    更新日期：2006-10-01 00:00:00

  • Phenotypic and functional characterization of mobilized peripheral blood CD34+ cells coexpressing different levels of c-Kit.

    abstract：:In this report we evaluated the exact expression pattern of c-Kit on mobilized peripheral blood (PB) CD34+ cells. Using a monoclonal antibody against CD117 antigen (95C3), flow cytometric analysis revealed that approximately 25% of the mobilized PB CD34+ cells coexpress c-Kit. This cell fraction showed a considerable ...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/s0145-2126(98)00002-2

    authors： Kuçi S,Taylor G,Neu S,Schumm M,Niethammer D,Handgretinger R

    更新日期：1998-04-01 00:00:00

  • c-myc locus amplification and the acquisition of trisomy 8 in the evolution of chronic myeloid leukaemia.

    abstract：:The biological progression of chronic myeloid leukaemia is often associated with secondary cytogenetic abnormalities but the molecular mechanisms underlying this progression are poorly understood. This study explores the association of c-myc gene amplification with the progression of chronic myeloid leukaemia in fourt...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/s0145-2126(98)00097-6

    authors： Jennings BA,Mills KI

    更新日期：1998-10-01 00:00:00

  • Acid hydrolases in B-chronic lymphocytic leukemia (B-CLL): a comparison with normal peripheral B lymphocytes and normal B-cell subset with the phenotype of B-leukaemic cells.

    abstract：:The acid phosphatase (AP), beta-glucuronidase (BG), and alpha-naphthyl-acetate esterase (ANAE) distribution and the morphology of stage 0 B-CLL lymphocytes were studied. The results were compared with the same hydrolase equipment and morphology of normal B-cell populations showing the B-CLL-like phenotype, and thus re...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/0145-2126(87)90074-9

    authors： Palestro G,Novero D,Godio L,Micca FB,Resegotti L,Stramignon A

    更新日期：1987-01-01 00:00:00

  • Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: utility of fluorescence in situ hybridization (FISH) in a case-control study.

    abstract：:We used fluorescence in situ hybridization (FISH) assays to identify t(14;18) translocations in archival paraffin-embedded tumor sections from non-Hodgkin lymphoma (NHL) cases enrolled in a population-based study. t(14;18) was identified in 54% of 152 cases, including 39% of diffuse large cell lymphomas (26 of 66 case...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2009.05.007

    authors： Chang CM,Schroeder JC,Huang WY,Dunphy CH,Baric RS,Olshan AF,Dorsey KC,Dent GA,Cerhan JR,Lynch CF,Rothman N,Cantor KP,Blair A

    更新日期：2010-02-01 00:00:00

  • Balance of NF-kappaB and p38 MAPK is a determinant of radiosensitivity of the AML-2 and its doxorubicin-resistant cell lines.

    abstract：:This study investigated radioresistance mechanisms in the doxorubicin-resistant acute myelogenous leukemia (AML)-2/DX100. AML-2/DX100 also showed resistance to radiation. AML-2/DX100 characterized by down-regulated catalase expression was supersensitive to exogenous hydrogen peroxide whereas they increased defense mec...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2006.11.006

    authors： Choi CH,Xu H,Bark H,Lee TB,Yun J,Kang SI,Oh YK

    更新日期：2007-09-01 00:00:00

  • Decreased chronic lymphocytic leukemia incidence in Asians in Los Angeles County.

    abstract：:Chronic lymphocytic leukemia (CLL) is rare in Asians living in Asia and possibly in US Asians. In contrast, CLL is the most common leukemia in whites. The basis for this ethnic and geographic variation is unknown. We compared average annual age-adjusted incidence rates (AAIR) of CLL diagnosed from 1972 to 1995 among L...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/s0145-2126(00)00038-2

    authors： Gale RP,Cozen W,Goodman MT,Wang FF,Bernstein L

    更新日期：2000-08-01 00:00:00

  • Cytotoxic effect of a lipophilic alkylating agent after incorporation into low density lipoprotein or emulsions: studies in human leukemic cells.

    abstract：:The use of low density lipoprotein (LDL) as drug carrier in acute myeloblastic leukemia chemotherapy is attractive due to high LDL uptake by leukemic cells. Lipid-based formulations, such as liposomes or microemulsions are promising alternatives. In the current study, we incorporated N-trifluoroacetyl-adriamycin-14-va...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2005.06.012

    authors： Masquelier M,Lundberg B,Peterson C,Vitols S

    更新日期：2006-02-01 00:00:00

  • Angiogenic activity of classical hematopoietic cytokines.

    abstract：:Hematopoiesis is regulated by several cytokines with pleiotropic activity. Several evidences have clearly demonstrated that these molecules, formerly regarded as specific for the hematopoietic system, also affect certain endothelial cell functions and that hematopoietic factors clearly influence angiogenesis. This rev...

    journal_title：Leukemia research

    pub_type： 杂志文章,评审

    doi：10.1016/j.leukres.2012.02.003

    authors： Ribatti D

    更新日期：2012-05-01 00:00:00

  • Ionic currents in multidrug resistant K562 human leukemic cells.

    abstract：:In this study, the expression and functional characterization of currents through the CFTR (cystic fibrosis transmembrane regulator) and ORCC (outwardly rectifying chloride channels) were determined in wild-type K562 chronic human leukemia cells (K562-WT) and in its resistant counterpart, the vincristine resistant cel...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2005.02.007

    authors： Assef YA,Cavarra SM,Damiano AE,Ibarra C,Kotsias BA

    更新日期：2005-09-01 00:00:00

  • Sharing post-AML consolidation supportive therapy with local centers reduces patient travel burden without compromising outcomes.

    abstract：:Acute myeloid leukemia (AML) is frequently treated with induction and consolidation chemotherapy. Consolidation chemotherapy can be delivered on an ambulatory basis, requiring some patients to travel long distances for treatment at specialized centers. We developed a shared care model where patients receive consolidat...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2017.05.023

    authors： Hershenfeld SA,Maki K,Rothfels L,Murray CS,Nixon S,Schimmer AD,Doherty MC

    更新日期：2017-08-01 00:00:00

  • Transcriptional deregulation of homeobox gene ZHX2 in Hodgkin lymphoma.

    abstract：:Recently, we identified a novel chromosomal rearrangement in Hodgkin lymphoma (HL), t(4;8)(q27;q24), which targets homeobox gene ZHX2 at the recurrent breakpoint 8q24. This aberration deletes the far upstream region of ZHX2 and results in silenced transcription pinpointing loss of activatory elements. Here, we have lo...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2011.10.019

    authors： Nagel S,Schneider B,Meyer C,Kaufmann M,Drexler HG,Macleod RA

    更新日期：2012-05-01 00:00:00

  • Myelodysplastic syndromes in patients under 50 years old: a single institution experience.

    abstract：:We report on our experience relating to 62 patients with myelodysplastic syndrome (MDS) aged less than 50 years, seen at our Institution and conservatively treated from July 1983 to December 2000. Patients demographics and clinical features at diagnosis were analysed for their prognostic value on survival and on risk ...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/j.leukres.2005.01.003

    authors： Breccia M,Mengarelli A,Mancini M,Biondo F,Gentilini F,Latagliata R,Mandelli F,Alimena G

    更新日期：2005-07-01 00:00:00

  • High-dose cytarabine-based consolidation shows superior results for older AML patients with intermediate risk cytogenetics in first complete remission.

    abstract：:We evaluated outcomes in two consecutive groups of AML patients age>60 years in CR after 7+3 induction therapy. Group 1 received consolidation with cytarabine 1.5g/m(2) q12h×6+daunorubicin for two cycles, while group 2 received consolidation with 7+3 followed by mitoxantrone+etoposide. For patients with intermediate-r...

    journal_title：Leukemia research

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.leukres.2013.01.001

    authors： Hassanein M,Atenafu EG,Schuh AC,Yee KW,Minden MD,Schimmer AD,Gupta V,Brandwein JM

    更新日期：2013-05-01 00:00:00

  • Bone and bone marrow interactions: hematological activity of osteoblastic growth peptide (OGP)-derived carboxy-terminal pentapeptide. II. Action on human hematopoietic stem cells.

    abstract：:Osteogenic growth peptide (OGP) is a peptide exerting regulatory effects on the bone and on bone marrow. The carboxy-terminal pentapeptide (OGP10-14) is the biologically active portion of OGP. We evaluated OGP10-14 hematopoietic activity performing colony-forming tests on human stem cells derived by bone marrow, perip...

    journal_title：Leukemia research

    pub_type： 杂志文章

    doi：10.1016/s0145-2126(02)00008-5

    authors： Fazzi R,Galimberti S,Testi R,Pacini S,Trasciatti S,Rosini S,Petrini M

    更新日期：2002-09-01 00:00:00