当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Human thioredoxin reductase from HeLa cells: selective alkylation of selenocysteine in the protein inhibits enzyme activity and reduction with NADPH influences affinity to heparin.

Human thioredoxin reductase from HeLa cells: selective alkylation of selenocysteine in the protein inhibits enzyme activity and reduction with NADPH influences affinity to heparin.

Abstract：

:Human thioredoxin reductase (TR) contains selenocysteine (Secys) in a redox center [cysteine (Cys)-497,Secys-498] near the C-terminus. The essential role of Secys in TR isolated from HeLa cells was demonstrated by the alkylation studies. Reaction of native NADPH reduced enzyme with bromoacetate at pH 6.5 inhibited enzyme activity 99%. Of the incorporated carboxymethyl (CM) group, 1.1 per subunit, >90% was in CM-Secys-498. Alkylation at pH 8 increased the stoichiometry to 1.6 per subunit with additional modification of the Cys-59, Cys-64 disulfide center. A minor tryptic peptide containing both CM-Cys-497 and CM-Secys-498 was isolated from enzyme alkylated at pH 6.5 or at pH 8. Preparations of TR isolated from HeLa cells grown in a fermentor under high aeration contained selenium-deficient enzyme species that had 50% lower activity. Decreasing oxygen to an optimal level increased cell yield, and fully active TR containing one Se per subunit was present. Reduction of fully active enzyme with tris-(2-carboxyethyl) phosphine converted it from a low to a high heparin affinity form. The tris-(2-carboxyethyl) phosphine-reduced enzyme was oxygen-sensitive and lost selenium and catalytic activity unless maintained under strictly anaerobic conditions. This enzyme could be converted to an oxygen-insensitive species by addition of NADPH, indicating that bound pyridine nucleotide is important for enzyme stability. An induced enzyme conformation in which the essential Secys is shielded from oxidative damage could explain these effects.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Gorlatov SN,Stadtman TC

doi

10.1073/pnas.95.15.8520

subject

Has Abstract

pub_date

1998-07-21 00:00:00

pages

8520-5

issue

15

eissn

0027-8424

issn

1091-6490

journal_volume

95

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Fatty acid-inducible ANGPTL4 governs lipid metabolic response to exercise.

    abstract：:Physical activity increases energy metabolism in exercising muscle. Whether acute exercise elicits metabolic changes in nonexercising muscles remains unclear. We show that one of the few genes that is more highly induced in nonexercising muscle than in exercising human muscle during acute exercise encodes angiopoietin...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1400889111

    authors： Catoire M,Alex S,Paraskevopulos N,Mattijssen F,Evers-van Gogh I,Schaart G,Jeppesen J,Kneppers A,Mensink M,Voshol PJ,Olivecrona G,Tan NS,Hesselink MK,Berbée JF,Rensen PC,Kalkhoven E,Schrauwen P,Kersten S

    更新日期：2014-03-18 00:00:00

  • Observations and interpretation of x-ray absorption edges in iron compounds and proteins.

    abstract：:X-ray absorption spectra near the Kalpha edge have been measured in various iron group compounds using the intense synchrotron radiation at the Stanford Synchrotron Research Project. In the cubic compounds KMF3 where M = Mn+2, Fe+2, Co+2, Ni+2, and Zn+2, well resolved lines were observed and assigned to the 1s leads t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.5.1384

    authors： Shulman GR,Yafet Y,Eisenberger P,Blumberg WE

    更新日期：1976-05-01 00:00:00

  • Regulation of p53 tetramerization and nuclear export by ARC.

    abstract：:Inactivation of the transcription factor p53 is central to carcinogenesis. Yet only approximately one-half of cancers have p53 loss-of-function mutations. Here, we demonstrate a mechanism for p53 inactivation by apoptosis repressor with caspase recruitment domain (ARC), a protein induced in multiple cancer cells. The ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0710017104

    authors： Foo RS,Nam YJ,Ostreicher MJ,Metzl MD,Whelan RS,Peng CF,Ashton AW,Fu W,Mani K,Chin SF,Provenzano E,Ellis I,Figg N,Pinder S,Bennett MR,Caldas C,Kitsis RN

    更新日期：2007-12-26 00:00:00

  • Regulatory T cells control tolerogenic versus autoimmune response to sperm in vasectomy.

    abstract：:Vasectomy is a well accepted global contraceptive approach frequently associated with epididymal granuloma and sperm autoantibody formation. To understand the long-term sequelae of vasectomy, we investigated the early immune response in vasectomized mice. Vasectomy leads to rapid epithelial cell apoptosis and necrosis...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1017615108

    authors： Wheeler K,Tardif S,Rival C,Luu B,Bui E,Del Rio R,Teuscher C,Sparwasser T,Hardy D,Tung KS

    更新日期：2011-05-03 00:00:00

  • Maintenance of an extrachromosomal plasmid vector in mouse embryonic stem cells.

    abstract：:We have constructed and characterized a polyoma virus-based plasmid that is maintained as an autonomously replicating extrachromosomal element (episome) in mouse embryonic stem (ES) cells. Plasmid pMGD20neo contains the polyoma origin of replication harboring a mutated enhancer (PyF101), a modified polyoma early regio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.5.1292

    authors： Gassmann M,Donoho G,Berg P

    更新日期：1995-02-28 00:00:00

  • Lithogenic hydrogen supports microbial primary production in subglacial and proglacial environments.

    abstract：:Life in environments devoid of photosynthesis, such as on early Earth or in contemporary dark subsurface ecosystems, is supported by chemical energy. How, when, and where chemical nutrients released from the geosphere fuel chemosynthetic biospheres is fundamental to understanding the distribution and diversity of life...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2007051117

    authors： Dunham EC,Dore JE,Skidmore ML,Roden EE,Boyd ES

    更新日期：2021-01-12 00:00:00

  • A gastrolith protein serving a dual role in the formation of an amorphous mineral containing extracellular matrix.

    abstract：:Despite the proclamation of Lowenstam and Weiner that crustaceans are the "champions of mineral mobilization and deposition of the animal kingdom," relatively few proteins from the two main calcification sites in these animals, i.e., the exoskeleton and the transient calcium storage organs, have been identified, seque...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0800193105

    authors： Shechter A,Glazer L,Cheled S,Mor E,Weil S,Berman A,Bentov S,Aflalo ED,Khalaila I,Sagi A

    更新日期：2008-05-20 00:00:00

  • All-carbon-based porous topological semimetal for Li-ion battery anode material.

    abstract：:Topological state of matter and lithium batteries are currently two hot topics in science and technology. Here we combine these two by exploring the possibility of using all-carbon-based porous topological semimetal for lithium battery anode material. Based on density-functional theory and the cluster-expansion method...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1618051114

    authors： Liu J,Wang S,Sun Q

    更新日期：2017-01-24 00:00:00

  • Characterization of potential selenium-binding proteins in the selenophosphate synthetase system.

    abstract：:Selenophosphate, an activated form of selenium that can serve as a selenium donor, is generated by the selD gene product, selenophosphate synthetase (SPS). Selenophosphate is required by several bacteria and by mammals for the specific synthesis of Secys-tRNA, the precursor of selenocysteine in selenoenzymes. Although...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0409042102

    authors： Ogasawara Y,Lacourciere GM,Ishii K,Stadtman TC

    更新日期：2005-01-25 00:00:00

  • Alarm calls evoke a visual search image of a predator in birds.

    abstract：:One of the core features of human speech is that words cause listeners to retrieve corresponding visual mental images. However, whether vocalizations similarly evoke mental images in animal communication systems is surprisingly unknown. Japanese tits (Parus minor) produce specific alarm calls when and only when encoun...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1718884115

    authors： Suzuki TN

    更新日期：2018-02-13 00:00:00

  • Molecular dissection of the evolution of carbapenem-resistant multilocus sequence type 258 Klebsiella pneumoniae.

    abstract：:Infections caused by drug-resistant bacteria are a major problem worldwide. Carbapenem-resistant Klebsiella pneumoniae, most notably isolates classified as multilocus sequence type (ST) 258, have emerged as an important cause of hospital deaths. ST258 isolates are predominantly multidrug resistant, and therefore infec...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1321364111

    authors： Deleo FR,Chen L,Porcella SF,Martens CA,Kobayashi SD,Porter AR,Chavda KD,Jacobs MR,Mathema B,Olsen RJ,Bonomo RA,Musser JM,Kreiswirth BN

    更新日期：2014-04-01 00:00:00

  • Dietary pesticides (99.99% all natural).

    abstract：:The toxicological significance of exposures to synthetic chemicals is examined in the context of exposures to naturally occurring chemicals. We calculate that 99.99% (by weight) of the pesticides in the American diet are chemicals that plants produce to defend themselves. Only 52 natural pesticides have been tested in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.19.7777

    authors： Ames BN,Profet M,Gold LS

    更新日期：1990-10-01 00:00:00

  • Distinct calcium-independent and calcium-dependent adhesion systems of chicken embryo cells.

    abstract：:Three criteria have been used to distinguish among different systems of embryonic cell adhesion: dependence on Ca2+, involvement of particular cell-surface molecules, and binding specificity. The characterization of the adhesion with respect to cell-surface molecules was carried out by using specific antibodies agains...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.1.387

    authors： Brackenbury R,Rutishauser U,Edelman GM

    更新日期：1981-01-01 00:00:00

  • The base rate principle and the fairness principle in social judgment.

    abstract：:Meet Jonathan and Elizabeth. One person is a doctor and the other is a nurse. Who is the doctor? When nothing else is known, the base rate principle favors Jonathan to be the doctor and the fairness principle favors both individuals equally. However, when individuating facts reveal who is actually the doctor, base rat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1524268113

    authors： Cao J,Banaji MR

    更新日期：2016-07-05 00:00:00

  • Human-modified landscapes alter mammal resource and habitat use and trophic structure.

    abstract：:The broad negative consequences of habitat degradation on biodiversity have been studied, but the complex effects of natural-agricultural landscape matrices remain poorly understood. Here we used stable carbon and nitrogen isotopes to detect changes in mammal resource and habitat use and trophic structure between pres...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1904384116

    authors： Magioli M,Moreira MZ,Fonseca RCB,Ribeiro MC,Rodrigues MG,Ferraz KMPMB

    更新日期：2019-09-10 00:00:00

  • The junctional multidomain protein AF-6 is a binding partner of the Rap1A GTPase and associates with the actin cytoskeletal regulator profilin.

    abstract：:The AF-6 protein is a multidomain protein that contains two potential Ras-binding domains within its N terminus. Because of this feature, AF-6 has been isolated in both two-hybrid and biochemical approaches and is postulated to be a potential Ras-effector protein. Herein, we show that it is specifically the first Ras-...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.16.9064

    authors： Boettner B,Govek EE,Cross J,Van Aelst L

    更新日期：2000-08-01 00:00:00

  • Long-term depression of glutamate-induced gamma-aminobutyric acid release in cerebellum by insulin-like growth factor I.

    abstract：:We tested the possibility that insulin-like growth factor I (IGF-I) acts as a neuromodulator in the adult cerebellar cortex since previous observations indicated that IGF-I is located in the olivo-cerebellar system encompassing the inferior olive and Purkinje cells. We found that conjoint administration of IGF-I and g...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.15.7386

    authors： Castro-Alamancos MA,Torres-Aleman I

    更新日期：1993-08-01 00:00:00

  • Rapid elimination of mature autoreactive B cells demonstrated by Cre-induced change in B cell antigen receptor specificity in vivo.

    abstract：:Developing autoreactive B cells edit their B cell antigen receptor (BCR) in the bone marrow and are clonally deleted when they fail to reexpress an innocent BCR. Here, inducible Cre-loxP-mediated gene inversion is used to change the specificity of the BCR on mature IgM+ IgD+ B cells in vivo to address the fate of lymp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.22.13171

    authors： Lam KP,Rajewsky K

    更新日期：1998-10-27 00:00:00

  • Partial purification and characterization of a macromolecule which enhances fluoride activation of adenylate cyclase.

    abstract：:Fluoride activation of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] is significantly enhanced (2 to 5 times) by a protein factor isolated from rat brain. The fluoride-dependent adenylate cyclase stimulator (FCS) is nondialyzable, trypsin-labile, and stable at 90 degrees C for 10 min. FCS stimula...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.8.4628

    authors： Rasenick MM,Bitensky MW

    更新日期：1980-08-01 00:00:00

  • Initiation factor IF-3-dependent binding of Escherichia coli ribosomes and N-formylmethionine transfer-RNA to rabbit globin messenger.

    abstract：:An initiation complex has been formed in high yields from E. coli ribosomes, 9S messenger RNA for rabbit hemoglobin, and N-formylmethionine-tRNA. Initiation factor IF-3 is required for the binding and puromycin is required for the release of fMet. Valyl-tRNA fails to bind to the second codon, whereas a mixture of 15 a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.7.1843

    authors： Noll M,Noll H,Lingrel JB

    更新日期：1972-07-01 00:00:00

  • Functional coupling of Ca2+ channels and ryanodine receptors in cardiac myocytes.

    abstract：:In skeletal muscle, dihydropyridine receptors are functionally coupled to ryanodine receptors of the sarcoplasmic reticulum in triadic or diadic junctional complexes. In cardiac muscle direct physical or functional couplings have not been demonstrated. We have tested the hypothesis of functional coupling of L-type Ca2...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.1.121

    authors： Sham JS,Cleemann L,Morad M

    更新日期：1995-01-03 00:00:00

  • N-terminal domain of complexin independently activates calcium-triggered fusion.

    abstract：:Complexin activates Ca(2+)-triggered neurotransmitter release and regulates spontaneous release in the presynaptic terminal by cooperating with the neuronal soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and the Ca(2+)-sensor synaptotagmin. The N-terminal domain of complexin is importa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1604348113

    authors： Lai Y,Choi UB,Zhang Y,Zhao M,Pfuetzner RA,Wang AL,Diao J,Brunger AT

    更新日期：2016-08-09 00:00:00

  • A human peripheral blood monocyte-derived subset acts as pluripotent stem cells.

    abstract：:We have identified, cultured, characterized, and propagated adult pluripotent stem cells (PSC) from a subset of human peripheral blood monocytes. These cells, which in appearance resemble fibroblasts, expand in the presence of macrophage colony-stimulating factor and display monocytic and hematopoietic stem cell marke...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0536882100

    authors： Zhao Y,Glesne D,Huberman E

    更新日期：2003-03-04 00:00:00

  • Nonlinear Laplacian spectral analysis for time series with intermittency and low-frequency variability.

    abstract：:Many processes in science and engineering develop multiscale temporal and spatial patterns, with complex underlying dynamics and time-dependent external forcings. Because of the importance in understanding and predicting these phenomena, extracting the salient modes of variability empirically from incomplete observati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1118984109

    authors： Giannakis D,Majda AJ

    更新日期：2012-02-14 00:00:00

  • Benzodiazepine/gamma-aminobutyric acid receptor deficit in the midbrain of the seizure-susceptible gerbil.

    abstract：:The density of benzodiazepine/gamma-aminobutyric acid receptor binding sites was lower in the midbrain of seizure-susceptible gerbils compared to control seizure-resistant gerbils. Binding of [3H]diazepam to high-affinity brain-specific sites in membrane homogenates of gerbil brain showed a 20-30% lower binding in mid...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.19.6701

    authors： Olsen RW,Wamsley JK,McCabe RT,Lee RJ,Lomax P

    更新日期：1985-10-01 00:00:00

  • Translation and a 42-nucleotide segment within the coding region of the mRNA encoded by the MAT alpha 1 gene are involved in promoting rapid mRNA decay in yeast.

    abstract：:In yeast, the mRNA encoded by the MAT alpha 1 gene is unstable (t1/2 = 5 min) and the mRNAs encoded by the ACT1 gene (t1/2 = 30 min) and the PGK1 gene (t1/2 = 45 min) are stable. To understand the RNA structural features that dictate mRNA decay rates in yeast, we have constructed PGK1/MAT alpha 1 and ACT1/MAT alpha 1 ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.7.2780

    authors： Parker R,Jacobson A

    更新日期：1990-04-01 00:00:00

  • A link between host plant adaptation and pesticide resistance in the polyphagous spider mite Tetranychus urticae.

    abstract：:Plants produce a wide range of allelochemicals to defend against herbivore attack, and generalist herbivores have evolved mechanisms to avoid, sequester, or detoxify a broad spectrum of natural defense compounds. Successful arthropod pests have also developed resistance to diverse classes of pesticides and this adapta...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1213214110

    authors： Dermauw W,Wybouw N,Rombauts S,Menten B,Vontas J,Grbic M,Clark RM,Feyereisen R,Van Leeuwen T

    更新日期：2013-01-08 00:00:00

  • Defining the remarkable structural malleability of a bacterial surface protein Rib domain implicated in infection.

    abstract：:Streptococcus groups A and B cause serious infections, including early onset sepsis and meningitis in newborns. Rib domain-containing surface proteins are found associated with invasive strains and elicit protective immunity in animal models. Yet, despite their apparent importance in infection, the structure of the Ri...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1911776116

    authors： Whelan F,Lafita A,Griffiths SC,Cooper REM,Whittingham JL,Turkenburg JP,Manfield IW,St John AN,Paci E,Bateman A,Potts JR

    更新日期：2019-12-09 00:00:00

  • Direct three-dimensional visualization of membrane disruption by amyloid fibrils.

    abstract：:Protein misfolding and aggregation cause serious degenerative conditions such as Alzheimer's, Parkinson, and prion diseases. Damage to membranes is thought to be one of the mechanisms underlying cellular toxicity of a range of amyloid assemblies. Previous studies have indicated that amyloid fibrils can cause membrane ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1206325109

    authors： Milanesi L,Sheynis T,Xue WF,Orlova EV,Hellewell AL,Jelinek R,Hewitt EW,Radford SE,Saibil HR

    更新日期：2012-12-11 00:00:00

  • Human Argonaute2 and Argonaute3 are catalytically activated by different lengths of guide RNA.

    abstract：:RNA interfering is a eukaryote-specific gene silencing by 20∼23-nucleotide (nt) microRNAs and small interfering RNAs that recruit Argonaute proteins to complementary RNAs for degradation. In humans, Argonaute2 (AGO2) has been known as the only slicer while Argonaute3 (AGO3) barely cleaves RNAs. Therefore, the intrinsi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2015026117

    authors： Park MS,Sim G,Kehling AC,Nakanishi K

    更新日期：2020-11-17 00:00:00