Abstract：
:Several models have been proposed for the quaternary structure of alpha-crystallin. Some suggest the subunits are arranged in concentric shells. Others propose that the subunits are in a micelle-like arrangement. However, none is able to satisfactorily account for all observations on the protein and the quaternary structure of alpha-crystallin remains to be established. In this review, factors contributing to the assembly and polymerization are examined in order to evaluate the different models. Consideration of the variations in particle size and molecular weight under different conditions leads to the conclusion that alpha-crystallin cannot be a micelle or a layered structure. Instead, it is suggested that the protein may be assembled from a 'monomeric' unit comprising eight subunits arranged in two tetramers with cyclic symmetry. The octameric unit is proposed to be disc-like particle with a diameter of 9.5 nm and a height of 3 nm. The larger particles, chains and sheet-like structures commonly observed are assembled from the octamers. Structural predictions indicate that the polypeptide may be folded into three independent domains which have different roles in the structural organization and functions of the protein. It is suggested that the tetramers are stabilized through interactions involving the second domain (residues 64-104) while assembly into the octamers and higher polymers requires hydrophobic interactions involving the N-terminal domain. Deletion of parts of this domain by site directed mutagenesis revealed that residues 46-63 play a critical role in the assembly. Current research aims to identify the specific amino acids involved.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Augusteyn RCdoi
10.1016/s0141-8130(98)00023-3subject
Has Abstractpub_date
1998-05-01 00:00:00pages
253-62issue
3-4eissn
0141-8130issn
1879-0003pii
S0141-8130(98)00023-3journal_volume
22pub_type
杂志文章abstract：:Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method. The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fo...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.06.077
更新日期：2016-10-01 00:00:00
abstract：:Naturally produced polyhydroxyalkanoates (PHAs) biopolymers have limited medical applications due to their brittle and hydrophobic nature. In this study poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) copolymer was produced using engineered Escherichia coli YJ101, and further functionalized with ascorbic ac...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.052
更新日期：2019-02-15 00:00:00
abstract：:The potential gastroprotection of polysaccharides (SP) isolated from maté (Ilex paraguariensis) leaves of different growth stages, under different sunlight conditions and of processing methods were evaluated. The SP consist of type I arabinogalactan (AG1) containing a (1→4)-linked β-Galp chain, with substituents of ar...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.04.038
更新日期：2013-08-01 00:00:00
abstract：:The interaction of novel oligo(L-lysine)-shelled dendrimers (G3-PLL) with DNA was studied by means of circular dichroism spectroscopy, dynamic light scattering, and melting behavior of double-stranded DNA. G3-PLLs having various oligo(L-lysine) (PLL) segment (n = 5-40) were successfully synthesized by graft-polymeriza...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2006.01.017
更新日期：2006-03-30 00:00:00
abstract：:To investigate the antitumor and antimetastatic polysaccharide from the mature leaves of green tea, GTE-II was purified using size exclusion chromatography. GTE-II consisted of 15 different sugars including rarely observed sugars such as 2-O-methyl-fucose, 2-O-methyl-xylose, apiose, aceric acid, 3-deoxy-d-manno-2-octu...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.02.043
更新日期：2017-06-01 00:00:00
abstract：:EPPS, a new heteropolysaccharide from Echinops pungens roots, was extracted by warm water (70 °C) and fortuitously purified using column chromatographies on DEAE-cellulose A52 and Sephadex G-100. Total carbohydrate content, specific optical rotation and molecular mass of EPPS were 98.3%, -88.2 and 65.7 kDa, respective...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.06.128
更新日期：2020-10-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.04.006
更新日期：2009-08-01 00:00:00
abstract：:The present study investigates the hypolipidemic effects of sulphated polysaccharide obtained from Codium fragile (CFSP) in induced obese rats (HFD). The results showed an increase in body weight of HFD rats by 21.56% as compared to control normal rats. Moreover, serum lipase activity underwent an increase which led t...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.04.017
更新日期：2017-09-01 00:00:00
abstract：:Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) was grown on crude glycerol from biodiesel production to produce a medium-chain-length polyhydroxyalkanoate (mcl-PHA), composed of 3-hydroxydodecanoate (43 ± 1.8 mol%), 3-hydroxydecanoate (29 ± 3.1 mol%), 3-hydroxytetradecanoate (12 ± 0.4 mol%), 3-hydroxyoctanoate...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.09.064
更新日期：2019-02-01 00:00:00
abstract：:In this study, the green synthesis of gum kondagogu capped gold nanoparticles (GK-GNPs) was prepared using a naturally available polysaccharide. The anionic gum capped GK-GNPs enabled the successful coupling of folic acid (FA) and fluorescein isothiocyanate (FITC) to produce a fluorescently labelled GNP (F2-GNP). F2-G...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.12.069
更新日期：2018-04-01 00:00:00
abstract：:For the cholesterol oxidase from Brevibacterium sp. M201008 was not stable as free enzyme form, it had been covalently immobilized onto functionalized sepharose particles that were activated with N-ethyl-N'-3-dimethylaminopropyl carbodiimide (EDC).The optimal conditions of enzyme immobilization were determined, and th...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.01.026
更新日期：2013-05-01 00:00:00
abstract：:A low molecular weight sulfated chitosan (SP-LMWSC) was isolated from the cuttlebone of Sepia pharaonis. Elemental analysis established the presence of C, H and N. The sulfation of SP-LMWSC was confirmed by the presence of characteristic peaks in FT-IR and FT-Raman spectra. The thermal properties of SP-LMWSC were stud...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.12.030
更新日期：2016-03-01 00:00:00
abstract：:The aim of this study was to improve the physicochemical stability of neohesperidin (NH) using nanoliposomal encapsulation in association with surface decoration strategy employing chitosan (CH) and pectin (P). Different nanoliposomal systems, i.e. NH-loaded nanoliposome (NH-NL), CH-coated NH-NL (CH-NH-NL), and P-coat...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.08.174
更新日期：2020-12-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.063
更新日期：2019-02-15 00:00:00
abstract：:Immunoscreening of a cDNA expression library of the Rhipicephalus (Boophilus) annulatus tick with purified rabbit anti-R annulatus salivary glands antigens polyclonal antibodies led to the identification of a 661bp sequence. The sequence includes an open reading frame of 543bp encoding a protein of 180 amino acids wit...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.08.006
更新日期：2010-12-01 00:00:00
abstract：:In most of the recent research on emulsions related to food products containing protein-polysaccharide interactions established via the Maillard reaction have been used as emulsifiers. Key challenges in such studies include long reaction times, uncontrollable extent of reaction, and protein denaturation and aggregatio...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.007
更新日期：2020-12-01 00:00:00
abstract：:The research aims the development and optimization of capecitabine loaded interpenetrating polymeric network by ionotropic gelation method using polymers locust bean gum and sodium alginate by QbD approach. FMEA was performed to recognize the risks influencing CQAs. BBD was applied to study the effect of factors (poly...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.04.123
更新日期：2018-08-01 00:00:00
abstract：:Using high pressure microfluidization, we prepared micro-fibrillated soybean cellulose (MFSC) and analyzed its morphology and structure. MFSC was then incorporated into low-methoxyl pectin (PC) to coat lactic acid bacteria (LAB) by ionotropic gelation, and the effects of PC-MFSC microgel on LAB survival in a simulated...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.04.161
更新日期：2020-05-06 00:00:00
abstract：:Liposomes, which release their contents in response to the concentration of glucose, were prepared by modifying the liposomal surface with the conjugate of poly(N-isopropylacrylamide-co-methacrylic acid-co-octadecylacrylate) (P(NIPAM-co-MAA-co-ODA)) and glucose oxidase (GOD). The maximum enzymatic activity of copolyme...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.06.008
更新日期：2009-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.133
更新日期：2019-03-15 00:00:00
abstract：:In this study, the physicochemical and in vitro digestion of millet starch with different moisture content after microwave treatment were investigated. The structure and property changes of native (NA) and microwave treated (MT) millet starches were characterized using Brabender viscometer, Fourier transforms infrared...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.046
更新日期：2019-08-01 00:00:00
abstract：:Asialoerythropoietin (asialo-EPO) is a desialylated form of human glycoprotein hormone erythropoietin (EPO), which has been reported to be neuro-, cardio-, and renoprotective in animal models of organ injuries. Since the current method of production of asialo-EPO from mammalian cell-made recombinant human EPO (rhuEPO(...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.10.033
更新日期：2015-01-01 00:00:00
abstract：:Alginate was grafted with NIPAM and NHMAM successfully, and a new responsive copolymer, alginate-g-P(NIPAM-co-NHMAM), was obtained. A novel dual responsive polysaccharide-based aerogel with thermo/pH sensitive properties was designed from the copolymer as drug controlled release system. The chemical structure of the c...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.03.166
更新日期：2018-07-15 00:00:00
abstract：SCOPE:In China, the root of M. officinalis has been widely used over thousands of years against a wide range of bone disease such as lumbago, limb-ache, sciatica and rheumatic arthralgia, and has tremendous medicinal value. But the bioactive constituents responsible for the osteoprotective effects in M. officinalis rem...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.082
更新日期：2018-12-01 00:00:00
abstract：:Agrobacterium tumefaciens uronate dehydrogenase (AtuUdh) belongs to the short-chain dehydrogenase superfamily, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. It is apparently required for the production of D-glucaric acid. AtuUdh-catalyzed reaction is reversible with dual substra...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.08.194
更新日期：2019-11-01 00:00:00
abstract：:Lanthanide nanoparticles and nanorods especially their biocompatible oxide forms like cerium oxide nanoparticles (CNPs) with therapeutic applications are used to cure neurological oxidative stresses. Thus it tempts to study their biocompatible aspects by interactions with several biologically significant molecules. In...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.067
更新日期：2020-08-01 00:00:00
abstract：:In this work, we have fabricated a novel amperometric cholesterol (CHO) biosensor because of the importance of determination of CHO levels in blood which is an important parameter for diagnosis and prevention of disease. To achieve this goal, cholesterol oxidase, cholesterol esterase and horseradish peroxidase were si...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.163
更新日期：2018-12-01 00:00:00
abstract：:This paper concerns the characterization of the chemical structure of a DP3 glucosamine oligomer. The assignments of nearly all protons are reported. Variations of 1H chemical shifts with pD and temperature are correlated to the pKa of amino groups, and not with substantial conformational changes. ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(91)90057-2
更新日期：1991-04-01 00:00:00
abstract：:It is well established that a sequence template along with the database is a powerful tool for identifying the biological function of proteins. Here, we describe a method for predicting the catalytic nature of certain proteins among the several protein structures deposited in the Protein Data Bank (PDB). For the prese...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.01.011
更新日期：2010-04-01 00:00:00
abstract：:Protein misfolding and aggregation has been implicated as the cause of more than 20 diseases in humans such as Alzheimer's and Parkinson's and systemic amyloidosis. Retardation of Aβ- 42 aggregation is considered as a promising and challenging strategy for developing effective therapeutics against Alzheimer's disease....
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.03.001
更新日期：2017-06-01 00:00:00
