Abstract:
:Bovine tuberculosis, which persists as a residual level of infection in many European countries, has implications not only for the economy of farming communities but also for human health. The aim of this study was to identify a common mycobacterial antigen which was recognized in bovine tuberculosis and to characterize the response to this antigen at the epitope level. A T-cell clone, phenotype CD4+, raised from an animal experimentally infected with Mycobacterium bovis was shown to proliferate in response to a panel of sonicates derived from different mycobacterial species indicating recognition of an antigen with broad specificity. This antigen was subsequently shown to be MPB59. Recognition of MPB59 at the epitope level was determined in experimental and field cases of bovine tuberculosis using a panel of synthetic peptides (20-mers with 10-residue overlaps) incorporating the signal sequence and mature protein. The results showed that in vitro interferon-gamma was predominantly produced in response to adjacent peptides numbers 10 and 11, suggesting that the dominant epitope was contained in the overlap, correlating to residues 101-110 (YYQSGLSIVM). This epitope was recognized by 54% of tuberculous cattle of mixed breeds, which suggests that it may be genetically permissive in terms of major histocompatibility complex presentation. Sequence analysis confirmed that there were only minor differences in the amino acid composition within this region for various mycobacterial species, which could explain the common T-cell recognition described in this study. Common recognition of this epitope indicates that it would have limited potential for use as a diagnostic reagent per se but may have potential for inclusion in a subunit vaccine.
journal_name
Immunologyjournal_title
Immunologyauthors
Lightbody KA,Girvin RM,Pollock DA,Mackie DP,Neill SD,Pollock JMdoi
10.1046/j.1365-2567.1998.00449.xsubject
Has Abstractpub_date
1998-03-01 00:00:00pages
314-22issue
3eissn
0019-2805issn
1365-2567journal_volume
93pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::The delayed-type-hypersensitivity (DTH) reaction observed in mice primed i.v. with low doses of sheep red blood cells is not detectable after priming with large doses. Estimation of the number of DTH mediating cells (DTH-C) was performed by titration (D50 determination) using local adoptive transfer of these cells mix...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-12-01 00:00:00
abstract::Innate immune responses vary in a circadian manner, and more recent investigations aim to understand the underlying molecular mechanisms. Cytokine production varies significantly over the course of a day depending on the time of stimulation by pathogens or Toll-like receptor ligands, and multiple signaling pathways li...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13053
更新日期:2019-06-01 00:00:00
abstract::Interleukin-15 (IL-15) is a proinflammatory cytokine that is overexpressed in rheumatoid arthritis (RA), a disease characterized by activation of monocytes/macrophages (MPhi), and by expansion of autoreactive CD4(+) T cells. We hypothesized that IL-15 plays a major role for this expansion of CD4(+) T cells and modulat...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02878.x
更新日期:2009-01-01 00:00:00
abstract::Degradation of the extracellular matrix plays an important role in rheumatoid articular destruction. Rheumatoid synovial fibroblasts secrete a large amount of matrix-degrading metalloproteinases (MMPs), which initiate tissue damage by proteolytic degradation of collagens and proteoglycans. Cytokines, such as interleuk...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.d01-789.x
更新日期:1996-12-01 00:00:00
abstract::The immunosuppressive effect of cyclosporin A (CS-A) was investigated in RIC-Sprague-Dawley rats. In vivo, CS-A totally abolished the formation of antibodies to the hapten dinitrophenyl (DNP) in rats immunized with DNP-keyhole limpet haemocyanin. In vitro, the effect of CS-A was investigated in spleen cell cultures st...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::Lymphoid cells in decidualized uterine endometrium in early human pregnancy may be essential for successful implantation and placentation. Endometrial granulocytes are prominent in early pregnancy decidua and express some T-cell associated surface antigens (CD2+, CD7+), but little is known of their function. We report...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::Compared with adults, the circulating Vγ2Vδ2 T-cell population in cord blood is present at low levels and does not show the strong bias for Vγ2-Jγ1.2 rearrangements. These features may be a result of limited exposure to stimulatory phosphoantigens, lack of T-cell-derived interleukin-2 (IL-2) or both. In cord blood mon...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12039
更新日期:2013-04-01 00:00:00
abstract::We examined whether protein-calorie (PC) and vitamin A (VA) deficiencies altered the intestinal localization of mesenteric lymph node (MLN) lymphoblasts using an adoptive lymphocyte transfer method. MLN cells from donor rats were labelled in vitro with [125I]-deoxyuridine and injected i.v. into various recipients. Twe...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Predisposition to autoimmune disorder in Lewis rats has been associated with abnormal hypothalamic regulation of circulating steroids, leading to inadequate suppression of T helper 1 (Th1) cell-mediated inflammatory reactions. In addition, autoimmune syndromes can be triggered within formerly resistant animals, follow...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00576.x
更新日期:1998-09-01 00:00:00
abstract::Interleukin-6 (IL-6) is an important B-cell growth and differentiation factor. IL-6 treatment of the human lymphoblastoid cell line, SKW6.4, leads to increased IgM production. We have previously shown that IL-6 induces activation of JAK1 and JAK2 in human B cell lines. A chimeric IL-6 receptor, comprised of the intrac...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.1997.00350.x
更新日期:1997-03-01 00:00:00
abstract::DNA breaks play an essential role in germinal centre B cells as intermediates to immunoglobulin class switching, a recombination process initiated by activation-induced cytidine deaminase (AID). Immunoglobulin gene hypermutation is likewise catalysed by AID but is believed to occur via single-strand DNA breaks. When i...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2008.03050.x
更新日期:2009-03-01 00:00:00
abstract::Bisphenol A (BPA) and p-nonylphenol (NP) are representative endocrine disruptors (EDs) that may have adverse effects on human health. The influence of these compounds on allergic immune responses remains unclear. In this study, we have examined the effects of BPA and NP on production of interleukin-4 (IL-4), a pro-inf...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01631.x
更新日期:2003-05-01 00:00:00
abstract::The L2C leukaemia is a B-lymphocytic neoplasm of strain 2 guinea-pigs, maintained by passaging in vivo. It synthesizes mu and lambda immunoglobulin chains. These combines to form monomeric (7S) IgM molecules which are inserted into the plasma membranes. From here they are shed as monomeric IgM and as a species of high...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-05-01 00:00:00
abstract::Supernatants of IL-2-activated mononuclear cells (MNC) that displayed an optimal lymphokine-activated killer (LAK) cell activity at 48-72 hr in culture were found to contain increased levels of tumour necrosis factor alpha (TNF alpha), interleukin-1 alpha (IL-1 alpha) and interferon-gamma (IFN-gamma) when compared wit...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::Neutrophils make up an essential part of the innate immune system, and are involved both in the initial responses to pathogens, and in orchestrating later immune responses. Neutrophils recognize pathogens through pattern-recognition receptors (PRRs), which are activated by microbial motifs. The Nod-like receptors (nuc...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2009.03212.x
更新日期:2010-05-01 00:00:00
abstract::Activated T cells, through the production of the receptor activator of NF-kappaB ligand (RANKL) cytokine, have been implicated in the osteoclast development and bone loss that are associated with autoimmune diseases such as rheumatoid arthritis. However, the cellular pathways that regulate the expression of RANKL and ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02858.x
更新日期:2008-11-01 00:00:00
abstract::This study demonstrated that the greater infectivity of stationary-phase promastigotes of Leishmania donovani is related to increased complement fixation on the parasite surface, resulting in increased binding to host mononuclear phagocytes (MPs) via complement type 3 receptors (CR3). The in vivo infectivity of log- a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
abstract::Macrophage (MΦ) polarization is triggered during the innate immune response to defend against microbial pathogens, but can also contribute to disease pathogenesis. In a previous study, we found that interleukin-15 (IL-15) -derived classically activated macrophages (M1 MΦ) have enhanced antimicrobial activity, whereas ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13013
更新日期:2019-02-01 00:00:00
abstract::Four types of lymphocyte chemotactic factor (LCF-a, -b, -c and -d) could be isolated from extract of 24-hr-old delayed-type hypersensitivity (DTH) skin reaction sites induced with purified protein derivative (PPD) in guinea-pigs by gel filtration on Sephadex G-100 followed by chromatography with DEAE-Sephadex. Partial...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract::Regulatory T (Treg) cells act to suppress activation of the immune system and thereby maintain immunological homeostasis and tolerance to self-antigens. The frequency and suppressing activity of Treg cells in general are high in different malignancies. We wanted to identify the role and regulation of CD4(+) CD25(+) ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12237
更新日期:2014-05-01 00:00:00
abstract::Immunization with intact IgG has allowed the isolation of four hybridomas producing antibodies recognizing epitopes expressed within subpopulations of human kappa light chains unrelated to known polymorphisms (Km) and previously defined V-region subgroups. The V-region-associated epitopes recognized are conformation-d...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
abstract::Resident glomerular macrophages from normal rats were isolated and grown through long-term cultures in order to obtain confluent cell monolayers. These cells have a secretory function as revealed by the production of a cytokine with a molecular weight similar to interleukin-1 (IL-1). The demonstration that rat glomeru...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
abstract::Fifty-seven serum samples positive for antibodies to double-stranded DNA (dsDNA), single-stranded DNA (ssDNA) or small nuclear ribonucleoproteins (snRNP) selected from patients with systemic lupus erythematosus and mixed connective tissue disease, were examined for the IgG subclass distribution of these autoantibodies...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::Crohn's disease (CD) is a chronic inflammatory disease of the gastrointestinal tract. Its pathogenesis is not completely understood, though the prevailing model is that the intestinal flora drives a strong intestinal T helper 1 (Th1)/Th17 type immune response and inflammation in the genetically susceptible host. This ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02839.x
更新日期:2008-10-01 00:00:00
abstract::To determine whether the paracrine secretion of interleukin-12 (IL-12) can efficiently stimulate the resistance to Mycobacterium avium complex (MAC) infection, 3T3 fibroblasts were stably transfected to secrete IL-12 (480 U/106 cells/48 hr) and their effect on MAC infection was investigated in genetically susceptible ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00796.x
更新日期:1999-07-01 00:00:00
abstract::We describe a high-throughput screening system to detect interactions between leucocyte surface proteins, taking into account that these interactions are usually of very low affinity. The method involves producing the extracellular regions of leucocyte proteins with tags so that they can be bound to nanoparticles to p...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2009.03153.x
更新日期:2010-01-01 00:00:00
abstract::Dibutyryl-cyclic adenosine 3'5'-monophosphate (cAMP) added to mouse spleen cell cultures under serum free conditions inhibited the stimulation of [3H]thymidine incorporation induced by T-cell mitogens much more than the one induced by B-cell mitogens. The inhibition was most impressive with phytohaemagglutinin, follow...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-05-01 00:00:00
abstract::Repetitive stimulation of naïve T cells by immature splenic dendritic cells (DC) can result in the differentiation of T-cell lines with regulatory properties. In the present study we identified a population of DC in the mucosae that exhibit the plasmacytoid phenotype, secrete interferon-alpha (IFN-alpha) following sti...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01606.x
更新日期:2003-04-01 00:00:00
abstract::Cholera toxin (CT) or its subunits were given orally to mice and division of intestinal intraepithelial lymphocytes (IEL) in vivo measured by double immunofluorescence using 5-bromo-2'-deoxyuridine (BRdU) and membrane alpha beta T-cell receptors (TCR) or gamma delta TCR staining in frozen sections. Cholera toxin (10 m...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.d01-721.x
更新日期:1996-09-01 00:00:00
abstract::The immunological outcome of infections and vaccinations is largely determined during the initial first days in which antigen-presenting cells instruct T cells to expand and differentiate into effector and memory cells. Besides the essential stimulation of the T-cell receptor complex a plethora of co-stimulatory signa...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12884
更新日期:2018-06-01 00:00:00