Expression of Rhizobium meliloti symbiotic promoters in Azorhizobium caulinodans.

Abstract:

:Plasmids containing Rhizobium meliloti symbiotic promoters P1 (promoter of nifHDK) and P2 (promoter of fixABCX) when mobilized into the cells of Azorhizobium caulinodans strain IRBG 46 showed strong expression of these promoters under free-living microaerobic as well as symbiotic conditions. Under free-living conditions microaerobiosis (3% or less O2) was found to be sufficient to activate these promoters; expression being higher at 1% than at 3% O2 concentration. Under symbiotic conditions the expression was much more stronger-with bacteroids in stem nodules showing higher expression than those in root nodules. Under both the conditions expression of the promoters in the native R. meliloti strain Rm102F34 was lower than that in the A. caulinodans strain IRBG 46. The results suggest a functional homology of these promoters in the heterologous background of A. caulinodans.

journal_name

Indian J Exp Biol

authors

Das A,Khanuja SP,Lodha ML

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

1331-5

issue

12

eissn

0019-5189

issn

0975-1009

journal_volume

35

pub_type

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