Abstract:
:CD23, the low-affinity IgE receptor, is up-regulated on interleukin (IL)-4-stimulated B cells and monocytes, with a concomitant increase in the release of soluble fragments of CD23 (sCD23) into the medium by proteolytic processing of the surface-bound intact CD23. The effect of inhibition of the processing of CD23 on IgE production in human and mouse cells and in a mouse model in vivo was evaluated. CD23 processing to sCD23 from RPMI 8866 (a human Epstein-Barr virus-transformed B cell line) cell membranes was inhibited by a broad-spectrum matrix-metalloprotease inhibitor, batimastat, with an IC50 of 0.15 microM. Batimastat also inhibited CD23 processing in whole RPMI 8866 cells as well as in IL-4-stimulated purified human monocytes with similar IC50. Batimastat inhibited IgE production from IL-4/anti-CD40-stimulated human tonsil B cells as well as mouse splenic B cells in a manner consistent with inhibition of CD23 processing. Release of soluble fragments of CD23 in the cell supernatants of tonsil B cells was inhibited over the concentration range of 1-10 microM batimastat and intact cell surface CD23 was increased on mouse splenic B cells in the presence of these concentrations of batimastat. IgE production of IL-4-stimulated human peripheral blood mononuclear cells was also blocked by 1-10 microM batimastat, again with comparable inhibition of sCD23 release over the same concentration range. Finally, in a mouse model of IgE production, batimastat inhibited IgE production in response to ovalbumin challenge as determined by serum IgE levels. Taken together, the data support a role of CD23 in IgE production and point to CD23 processing to sCD23 as a therapeutically relevant control point in the regulation of IgE synthesis.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Christie G,Barton A,Bolognese B,Buckle DR,Cook RM,Hansbury MJ,Harper GP,Marshall LA,McCord ME,Moulder K,Murdock PR,Seal SM,Spackman VM,Weston BJ,Mayer RJdoi
10.1002/eji.1830271221subject
Has Abstractpub_date
1997-12-01 00:00:00pages
3228-35issue
12eissn
0014-2980issn
1521-4141journal_volume
27pub_type
杂志文章abstract::Beta1 integrins can provide T cell co-stimulation, but little is known concerning their downstream signaling pathways. We found that Pyk2, a focal adhesion kinase-related tyrosine kinase, is regulated by beta1 integrin signaling in human T cells. Stimulation of Jurkat T cells with the alpha4beta1 integrin ligand VCAM-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3867::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::It is widely accepted that females have superior immune responses than males, but the ways by which sex hormones may enhance T cell responses are still poorly understood. In the present study, we analyzed the effect of estrogens on CD4 T cell activation and differentiation after immunization with exogenous antigens. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/immu.200310027
更新日期:2003-02-01 00:00:00
abstract::The viral CC chemokine macrophage inhibitory protein-II (vMIP-II) encoded by human herpes virus 8 (HHV-8) binds to multiple chemokine receptors, however, its ability to control the initial recruitment of specific leukocyte subtypes from the peripheral circulation has not been fully clarified. Here we show that vMIP-II...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200108)31:8<2458::aid-immu24
更新日期:2001-08-01 00:00:00
abstract::Secretion of proinflammatory mediators by activated macrophages plays an important role in the immune response to Trypanosoma cruzi. We have previously reported that AgC10, a glycosylphosphatidylinositol-anchored mucin from T. cruzi, inhibits TNF secretion by activated macrophages (de Diego, J., Punzon, C., Duarte, M....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324660
更新日期:2004-06-01 00:00:00
abstract::The NZB mouse is genetically predisposed to the development of autoimmune disease that resembles the human autoimmune systemic lupus erythematosus and autoimmune hemolytic anemia, with increased titers of anti-DNA, and Coombs' autoantibodies. The various autoimmune traits are controlled separately by a limited number ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210415
更新日期:1991-04-01 00:00:00
abstract::The immunosuppressor sanglifehrin A (SfA) is a member of a family of immunophilin cyclophilin A-binding molecules and does not inhibit calcineurin activity. Sanglifehrin A inhibits M-CSF-dependent macrophage proliferation by arresting the G1 phase of the cell cycle but does not affect cell viability. This immunosuppre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535270
更新日期:2006-09-01 00:00:00
abstract::We have analyzed the T cell receptor (TcR) V alpha and TcR V beta regions in the spontaneous mouse model for insulin-dependent diabetes mellitus, the NOD mouse, and compared it to the regions in the two sister strains, the NON and CTS strains. Based on restriction fragment length polymorphism analysis the TcR V alpha ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220337
更新日期:1992-03-01 00:00:00
abstract::Mycoplasma arthritidis produces an as yet undefined soluble molecule (MAS) that has a potent mitogenic effect on T cells of several species. We have used cloned human cytotoxic and proliferative T lymphocytes to dissect the molecular mechanism of T cell activation by this mitogen. Reactivity to MAS is clonally express...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181112
更新日期:1988-11-01 00:00:00
abstract::Adoptive T-cell therapy (ATCT) can result in tumor rejection, yet the behavior and fate of the introduced T cells remain unclear. We developed a novel bioluminescence mouse model, which enabled highly sensitive detection of T-cell signals at the single-cell level. Transferred T cells preferentially accumulated within ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141452
更新日期:2011-11-01 00:00:00
abstract::The cytokine interleukin 1 (IL 1) plays an important role in the induction of IL 2 secretion and high-affinity IL 2 receptor (IL 2R) expression by T cells. The events that follow binding of IL 1 to IL 1R, however, are still unknown. In this study we describe two variants of the murine thymoma EL4 (5D3 and D6/76) that ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190217
更新日期:1989-02-01 00:00:00
abstract::The thymus provides the microenvironment in which thymocytes develop into mature T cells, and interactions with thymic stromal cells are thought to provide the necessary signals for thymocyte maturation. Recognition of self-MHC by T cells is a basic requirement for mature T-cell functions, and those thymocytes that do...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201142338
更新日期:2012-07-01 00:00:00
abstract::To determine whether antigen (Ag)-specific cytotoxic T lymphocytes are generated during experimental Candida albicans infection, purified L3T4+ and Ly-2+ lymphocytes from immunized mice were cultured in the presence of syngeneic accessory cells, C. albicans Ag, and interleukin 2. Yeast-infected bone marrow macrophages...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210636
更新日期:1991-06-01 00:00:00
abstract::Disseminated infections by the opportunistic yeast Cryptococcus neoformans are characterized by accumulation in tissues of glucuronoxylomannan (GXM), the major component of the capsular polysaccharide. We investigated binding, uptake, and disposal of GXM by peripheral blood neutrophils and monocytes, and the effect of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323388
更新日期:2003-04-01 00:00:00
abstract::A T3 complex-bearing subpopulation was characterized within an in vivo cycling T4-8- early thymocyte compartment which contains cells constitutively expressing interleukin 2 and transferrin receptors. We show differentiation in vitro of both mature subsets of thymocytes (T3+4+8- and T3+4-8+) from the above T4-8- compa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160611
更新日期:1986-06-01 00:00:00
abstract::In the present study, we have investigated the anatomic distribution and the function of different populations of HIV-1- and cytomegalovirus (CMV)-specific memory CD8 T cells. The different populations of virus-specific memory CD8 T cells were distinguished on the basis of the expression of CD45RA and CCR7, and the co...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200212)32:12<3756::AID-IMMU3756>
更新日期:2002-12-01 00:00:00
abstract::The C-terminal fragment of merozoite surface protein-1 (MSP-1) of the mouse malaria parasite Plasmodium chabaudi chabaudi (AS) stimulates a weak CD4 T cell response when compared to the response to a more structurally simple region of the molecule. The tertiary structure of the C-terminal region of MSP-1 is maintained...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200101)31:1<72::aid-immu72
更新日期:2001-01-01 00:00:00
abstract::Previous studies have shown that the fecal flora of patients with Crohn's disease (CD) differed from the flora of healthy subjects by a higher number of anaerobic gram-positive coccoid rods. Sera from patients with CD agglutinated four strains of coccoid rods (Me44, C18, Me46 and Me47) more frequently and stronger tha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150823
更新日期:1985-08-01 00:00:00
abstract::This study was conducted to further characterize the effector cells of experimental allergic encephalomyelitis (EAE) which are activated in vitro when spleen cells from Lewis rats previously immunized with myelin basic protein and adjuvant are cultured with antigen prior to transfer to syngeneic recipients. The effect...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120519
更新日期:1982-05-01 00:00:00
abstract::Cells present in the polyoma virus-induced murine ascites tumor SEYF-a showed the capacity to fix soluble immune complexes of ovalbumin anti-ovalbumin. The fixation could be inhibited by preincubating the cells with antisera directed against H-2 antigens and with syngeneic anti-tumor antibodies. The latter did not rea...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060810
更新日期:1976-08-01 00:00:00
abstract::CD40 ligand (CD40L) is a 33-kDa type II membrane glycoprotein induced on T cells upon activation. CD40L has previously been shown to induce proliferation of resting B cells, immunoglobulin (Ig) secretion from B cells cultured with cytokines and cytokine secretion and tumoricidal activity from monocytes. In this report...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230941
更新日期:1993-09-01 00:00:00
abstract::The structure of two T-immunogenic peptides, one from the gag p24 protein of the human immunodeficiency virus, the other from the 11.1 gene product of Plasmodium falciparum, was studied by circular dichroism spectroscopy in various pH and solvent conditions. Although both sequences are predicted to adopt an alpha-heli...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191033
更新日期:1989-10-01 00:00:00
abstract::Lymphocyte proliferation in response to monocytes pulsed with an antigenic extract of Candida albicans was measured in vitro and the effects of modifying major histocompatibility complex (MHC) class II antigen expression at the surface of the antigen-presenting cells was investigated. The study shows that no simple co...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160410
更新日期:1986-04-01 00:00:00
abstract::The distribution of idiotype-positive plaques in mice of various strains injected with T-independent (TI) and T-dependent conjugates of p-azobenzenearsonate (Ars) has been investigated. With maturation of the responses there is a progressive loss of dominance of the major cross-reactive idiotype (CRI) in A/J and C.AL-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120302
更新日期:1982-03-01 00:00:00
abstract::Chronic skin inflammation resulting from a defective epidermal barrier is a hallmark of atopic dermatitis (AD). We previously demonstrated that mice lacking FGF receptors 1 and 2 in keratinocytes (K5-R1/R2 mice) develop an AD-like chronic dermatitis as a result of an impaired epidermal barrier. Here, we show that γδ T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545675
更新日期:2015-09-01 00:00:00
abstract::The cloned human gamma 1 heavy chain gene (HIG1) was scarcely expressed in the stable transformants of a mouse fibroblast line, L cell or a T cell line, EL4, and no gamma 1 heavy chain was produced in these cells. Upon treatment with cycloheximide or other kinds of protein synthesis inhibitors, the transcription of HI...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170906
更新日期:1987-09-01 00:00:00
abstract::Restriction fragment length polymorphism has been compared between the Igh loci of C57BL/6 and MOLF/EI (Mus musculus molossinus) mice utilizing probes which detect the C gamma 2b gene and genes from nine VH-gene families. Distinct restriction site patterns were found for the CH genes and for VH families PC7183, Q52 an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170920
更新日期:1987-09-01 00:00:00
abstract::Previously, we found that co-expression of CD25 and TNFR2 identified the most suppressive subset of mouse Treg. In this study, we report that human peripheral blood (PB) FOXP3(+) cells present in CD25(high), CD25(low) and even CD25(-) subsets of CD4(+) cells expressed high levels of TNFR2. Consequently, TNFR2-expressi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940022
更新日期:2010-04-01 00:00:00
abstract::Antibodies against bacterial capsular polysaccharides play a critical protective role. Responses to these antigens can occur without the help or control of T cells and are associated with marginal zone (MZ) B cells. Capsular antigens are diverse and some cross-react with self-carbohydrate epitopes. This diversity may ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/immu.200310003
更新日期:2003-02-01 00:00:00
abstract::Dendritic cells (DC) play an essential role in the induction of primary immune responses; however, very little information is available on cytokine production by DC. Here we determined the cytokine gene expression profile of two immortalized DC clones, CB1 and D2SC/1, both generated from mouse spleen but differing in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241039
更新日期:1994-10-01 00:00:00
abstract::Dendritic cells (DC) are sentinels of the immune system, transporting antigens from the periphery to secondary lymphoid organs. This study investigates the interactions of DC with B cells for the induction of anti-viral neutralizing antibody responses. Using the vesicular stomatitis virus glycoprotein (VSV-G) as a mod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200001)30:1<185::AID-IMMU185>3.0
更新日期:2000-01-01 00:00:00