Ultrastructural localization of histamine in human basophils and mast cells; changes associated with anaphylactic degranulation and recovery demonstrated with a diamine oxidase-gold probe.


:Subcellular sites of histamine in purified human lung mast cells and human peripheral blood basophils were determined using a newly developed enzyme-gold affinity method for ultrastructural analysis. The evolving morphologies of regulated secretion and recovery from regulated secretion in each cell showed that granule stores of histamine were released at early times after stimulation and re-accumulated at later times post-stimulus. Renewal of histamine granule stores occurred by multiple mechanisms including synthesis and conservation of granule materials and membranes.






Dvorak AM




Has Abstract


1997-01-01 00:00:00




34 Suppl










  • Possible effect of landscape design on IgE recognition profiles of two generations revealed with micro-arrayed allergens.

    abstract::The aim of this study was to investigate possible effects of landscape design on the IgE sensitization profile toward inhalant allergens in patients with respiratory allergy from Uzbekistan where green areas have been changed during the last two decades by a State program. Sera from two different generations of Uzbek ...


    pub_type: 杂志文章


    authors: Garib V,Wollmann E,Djambekova G,Lemell P,Kmenta M,Berger U,Zieglmayer P,Valenta R

    更新日期:2017-10-01 00:00:00

  • Identification of B-cell epitopes of Bet v 1 involved in cross-reactivity with food allergens.

    abstract:BACKGROUND:The pollen-food syndrome (PFS) is an association of food allergies to fruits, nuts, and vegetables in patients with pollen allergy. Mal d 1, the major apple allergen, is one of the most commonly associated food allergens for birch pollen-allergic patients suffering from PFS. Although the reactions are due to...


    pub_type: 杂志文章


    authors: Klinglmayr E,Hauser M,Zimmermann F,Dissertori O,Lackner P,Wopfner N,Ferreira F,Wallner M

    更新日期:2009-04-01 00:00:00

  • Cord blood Tregs with stable FOXP3 expression are influenced by prenatal environment and associated with atopic dermatitis at the age of one year.

    abstract:BACKGROUND:Regulatory T cells (Tregs) with stable FOXP3 expression are characterized by a specific demethylated region in the FOXP3 gene (Treg-specific demethylated region, TSDR). The aim of this study was to analyse the influence of prenatal factors on cord blood Treg numbers, as detected by changes in the TSDR demeth...


    pub_type: 杂志文章


    authors: Hinz D,Bauer M,Röder S,Olek S,Huehn J,Sack U,Borte M,Simon JC,Lehmann I,Herberth G,LINA study group.

    更新日期:2012-03-01 00:00:00

  • Anaphylaxis caused by Hymenoptera stings: from epidemiology to treatment.

    abstract::Hymenoptera venom allergy occasionally causes fatal reactions. The prevalence of systemic reactions (SRs) is 0.3-8.9%, with anaphylaxis in 0.3-42.8% of cases. Factors contributing to reaction severity include older age, insect type, a previous less severe SR, preexisting diseases, concomitant treatments, mast cell dis...


    pub_type: 杂志文章


    authors: Bilò MB

    更新日期:2011-07-01 00:00:00

  • Asthma in the desert: spectrum of the sensitizing aeroallergens.

    abstract:BACKGROUND:Bronchial asthma is common in Kuwait, a desert country, but the sensitizing allergens are uncertain. This study investigated the sensitizing allergens in Kuwaiti patients with extrinsic asthma. METHODS:A total of 553 asthmatics (male:female ratio: 1.4; mean age: 31.7 years [range 3-76 years]) and 112 matche...


    pub_type: 杂志文章


    authors: Ezeamuzie CI,Thomson MS,Al-Ali S,Dowaisan A,Khan M,Hijazi Z

    更新日期:2000-02-01 00:00:00

  • Effects of BCG revaccination on asthma.

    abstract::In a study conducted 1 year ago, we found that Th1 immune enhancement following Bacille Calmette-Guérin (BCG) vaccination effectively suppressed human asthma. To investigate whether revaccination would further improve lung function, BCG vaccine was given again. Current lung function tended to improve in the Repeated B...


    pub_type: 临床试验,杂志文章


    authors: Choi IS,Koh YI

    更新日期:2003-11-01 00:00:00

  • Weight loss interventions in asthma: EAACI evidence-based clinical practice guideline (part I).

    abstract:BACKGROUND:Asthma and obesity are chronic multifactorial conditions that are associated with gene-environment interaction and immune function. Although the data are not fully consistent, it seems that obesity increases the risk of asthma and compromises asthma control. OBJECTIVE:To investigate the impact that weight c...


    pub_type: 杂志文章,meta分析


    authors: Moreira A,Bonini M,Garcia-Larsen V,Bonini S,Del Giacco SR,Agache I,Fonseca J,Papadopoulos NG,Carlsen KH,Delgado L,Haahtela T

    更新日期:2013-04-01 00:00:00

  • A microtiter assay for activation of eosinophils. Simultaneous monitoring of eosinophil adhesion and degranulation.

    abstract::A simple microtiter assay for eosinophil activation is described. The assay used 1000-4000 eosinophils/microtiter well, and the design allows for a separate or simultaneous quantitative assessment of eosinophil adhesion to protein-coated microtiter wells and degranulation after stimulation with eosinophil-activating f...


    pub_type: 杂志文章


    authors: Reimert CM,Skov PS,Poulsen LK

    更新日期:1998-02-01 00:00:00

  • Terbutaline depot tablets in asthma. A clinical evaluation.

    abstract::A sustained release preparation of terbutaline sulphate has been formulated (Bricanyl depot tablets) in order to extend the duration and accordingly change the dosage regimen to twice a day. This presentation gives a summary of a clinical trial performed in order to study effect and side effects of terbutaline depot t...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Taudorf E,Bundgaard A,Fagerström PO,Weeke E,Weeke B

    更新日期:1981-10-01 00:00:00

  • Allergy to jackfruit: a novel example of Bet v 1-related food allergy.

    abstract:BACKGROUND:Jackfruit allergy has been reported just once. It is unknown whether this food allergy is caused by direct sensitization or cross-sensitization to pollen allergens. OBJECTIVE:Establish whether jackfruit allergy is linked to birchpollen allergy. METHODS:Two jackfruit allergic patients and five patients with...


    pub_type: 杂志文章


    authors: Bolhaar ST,Ree R,Bruijnzeel-Koomen CA,Knulst AC,Zuidmeer L

    更新日期:2004-11-01 00:00:00

  • Langerhans cells mediate the skin-induced tolerance to ovalbumin via Langerin in a murine model.

    abstract:BACKGROUND:Epicutaneous sensitization is an important route of immunization for allergens in atopic diseases; however, studies have also shown that application with protein on the intact skin induces antigen-specific tolerance. Langerhans cells (LCs) play an immunosuppressive role in several inflammatory skin diseases ...


    pub_type: 杂志文章


    authors: Luo Y,Wang S,Liu X,Wen H,Li W,Yao X

    更新日期:2019-09-01 00:00:00

  • Plasma protein profiles in early asthmatic responses to inhalation allergen challenge.

    abstract::Although mediators, such as lipids, cytokines, and chemokines, are related to the appearance of an IPR, there has been no reliable indicator to predict conditions for the appearance of an IPR. In this study, we adopted a proteomic approach to investigate the pathogenesis at the level of the plasma proteins and to deve...


    pub_type: 杂志文章


    authors: Rhim T,Choi YS,Nam BY,Uh ST,Park JS,Kim YH,Paik YK,Park CS

    更新日期:2009-01-01 00:00:00

  • Prick test: evolution towards automated reading.

    abstract::The prick test is one of the most common medical methods for diagnosing allergies, and it has been carried out in a similar and laborious manner over many decades. In an attempt to standardize the reading of the test, many researchers have tried to automate the process of measuring the allergic reactions found by deve...


    pub_type: 杂志文章,评审


    authors: Justo X,Díaz I,Gil JJ,Gastaminza G

    更新日期:2016-08-01 00:00:00

  • Comparison of skin tests and RAST for the diagnosis of bee sting allergy.

    abstract::Skin tests and estimation of specific IgE-antibodies by the RAST are evaluated as diagnostic procedures in bee sting hypersensitivity with the followiny extracts. (2) With bee venom results of skin tests and RAST correspond in 82%, with bee wholebody extracts in 68%. (3) Both with skin tests and the RAST with bee veno...


    pub_type: 杂志文章


    authors: Patrizzi R,Müller U,Yman L,Hoigné R

    更新日期:1979-08-01 00:00:00

  • Fenoterol, a selective beta 2-adrenergic agonist, and inhibition of IgE-mediated basophil histamine release.

    abstract::The present study was undertaken to evaluate the effect of fenoterol, a selective beta 2-adrenergic agonist, on basophil histamine release. Fenoterol at 10(-7) to 10(-3) M did not inhibit the release of histamine induced by Dermatophagoides farinae extract (D.f.) from leukocytes from allergic patients sensitive to mit...


    pub_type: 杂志文章


    authors: Morita Y,Miyamoto T

    更新日期:1987-10-01 00:00:00

  • Current perspective on eicosanoids in asthma and allergic diseases: EAACI Task Force consensus report, part I.

    abstract::Eicosanoids are biologically active lipid mediators, comprising prostaglandins, leukotrienes, thromboxanes, and lipoxins, involved in several pathophysiological processes relevant to asthma, allergies, and allied diseases. Prostaglandins and leukotrienes are the most studied eicosanoids and established inducers of air...


    pub_type: 杂志文章


    authors: Sokolowska M,Rovati GE,Diamant Z,Untersmayr E,Schwarze J,Lukasik Z,Sava F,Angelina A,Palomares O,Akdis CA,O'Mahony L,Sanak M,Dahlen SE,Woszczek G

    更新日期:2021-01-01 00:00:00

  • Oral disodium cromoglycate treatment of atopic dermatitis.

    abstract::Fourteen adults and 10 children with active atopic dermatitis entered this double blind cross-over study of oral disodium cromoglycate (DSCG) (adults 200 mg qid, children 100 mg qid) compared with placebo. Oral DSCG and placebo were given for 6 weeks in random order. According to the investigators' assessments of ecze...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Lindskov R,Knudsen L

    更新日期:1983-04-01 00:00:00

  • Allergen sensitization and exposure to irritants in infancy.

    abstract::We investigated the relationship between residence in the neighbourhood of an aluminium smelter and the prevalence of atopy in schoolchildren (7-13 years of age). Atopy was assessed in 556 of the 620 participants by a skin prick test with eight common aeroallergens. The median exposures to sulphur dioxide and fluoride...


    pub_type: 杂志文章


    authors: Søyseth V,Kongerud J,Boe J

    更新日期:1996-10-01 00:00:00

  • Advances and novel developments in environmental influences on the development of atopic diseases.

    abstract::Although genetic factors play a role in the etiology of atopic disease, the rapid increases in the prevalence of these diseases over the last few decades suggest that environmental, rather than genetic factors are the driving force behind the increasing prevalence. In modern societies, there is increased time spent in...


    pub_type: 杂志文章,评审


    authors: Alkotob SS,Cannedy C,Harter K,Movassagh H,Paudel B,Prunicki M,Sampath V,Schikowski T,Smith E,Zhao Q,Traidl-Hoffmann C,Nadeau KC

    更新日期:2020-10-09 00:00:00

  • Cat, dog, and house-dust-mite allergen levels of house dust in Finnish apartments.

    abstract::The presence of indoor allergens in Finnish homes was studied for the first time. Dust samples (n = 30) were collected by vacuuming a 1 m2 area from a living-room carpet in 30 apartments divided into three groups: homes with cats (n = 10), homes with dogs (n = 10), and homes without pets (n = 10). The levels of major ...


    pub_type: 杂志文章


    authors: Raunio P,Pasanen AL,Reiman M,Virtanen T

    更新日期:1998-02-01 00:00:00

  • Short-term venom immunotherapy induces desensitization of FcεRI-mediated basophil response.

    abstract:BACKGROUND:The precise immunological mechanisms for the early clinical protection of venom immunotherapy (VIT) have not yet been explained. Our aim was to evaluate whether high-affinity IgE receptor (FcεRI) and the related basophil function have a role in the induction of short-term VIT protection. METHODS:We included...


    pub_type: 杂志文章


    authors: Čelesnik N,Vesel T,Rijavec M,Šilar M,Eržen R,Košnik M,Kloft Žitnik SE,Avčin T,Korošec P

    更新日期:2012-12-01 00:00:00

  • House-dust mites and associated environmental conditions in Danish homes.

    abstract::The concentration of house-dust mites (Dermatophagoides pteronyssinus) was investigated in 96 Danish homes with one or more members suffering from asthma. The air-exchange rates, humidity, and temperature were measured in all the homes. A positive correlation was found between indoor air humidity and mite concentratio...


    pub_type: 杂志文章


    authors: Harving H,Korsgaard J,Dahl R

    更新日期:1993-02-01 00:00:00

  • Montelukast 10 mg improves nasal function and nasal response to aspirin in ASA-sensitive asthmatics: a controlled study vs placebo.

    abstract:UNLABELLED:Aspirin-induced asthma (AIA) is a clinical syndrome characterized by acute airway reaction to aspirin and other nonsteroidal anti-inflammatory drugs (NSAIDS). The most recent etiological hypothesises is that an overexpression of the enzyme LTC(4) synthase occurs in AIA, with the consequent production of sulf...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Micheletto C,Tognella S,Visconti M,Pomari C,Trevisan F,Dal Negro RW

    更新日期:2004-03-01 00:00:00

  • Clinical correlates of rhinovirus infection in preschool asthma.

    abstract:BACKGROUND:Investigation of preschool asthma is important since not all children outgrow their illness during this age. Data are scarce on the role of rhinovirus (RV) infections in this patient group. OBJECTIVES:To investigate the role of RV infections in preschool asthma: (i) susceptibility factors, (ii) clinical cou...


    pub_type: 杂志文章


    authors: Jartti T,Liimatainen U,Xepapadaki P,Vahlberg T,Bachert C,Finotto S,Kowalski ML,Sobanska A,Lukkarinen H,Pasioti M,Vuorinen T,Zhang N,Zimmermann T,Papadopoulos NG

    更新日期:2021-01-01 00:00:00

  • Safety of rush insect venom immunotherapy. The results of a retrospective study in 178 patients.

    abstract:BACKGROUND:Venom immunotherapy (VIT) is regarded as an established and efficient treatment of Hymenoptera venom allergy. Different frequencies of side-effects have been reported in the literature, depending on the hyposensitization regimen (conventional outpatient programme, rush immunotherapy, ultra rush immunotherapy...


    pub_type: 杂志文章


    authors: Wenzel J,Meissner-Kraemer M,Bauer R,Bieber T,Gerdsen R

    更新日期:2003-11-01 00:00:00

  • Activation of KIT modulates the function of tumor necrosis factor-related apoptosis-inducing ligand receptor (TRAIL-R) in mast cells.

    abstract:BACKGROUND:Mastocytosis is characterized by the accumulation of mast cells (MCs) associated with activating mutations of KIT. Tumor necrosis factor-related apoptosis-inducing ligand receptors (TRAIL-Rs) are preferentially expressed on neoplastic cells and induce the extrinsic apoptotic pathway. Recent studies reported ...


    pub_type: 杂志文章


    authors: Förster A,Grotha SP,Seeger JM,Rabenhorst A,Gehring M,Raap U,Létard S,Dubreuil P,Kashkar H,Walczak H,Roers A,Hartmann K

    更新日期:2015-07-01 00:00:00

  • Polymorphisms in interleukin-1 receptor-associated kinase 4 are associated with total serum IgE.

    abstract:BACKGROUND:Serum immunoglobulin E (IgE) level is recognized to be under strong genetic control, but the causal and susceptibility genes remain to be identified. We sought to investigate the association between single nucleotide polymorphisms (SNPs) in the Toll-like receptor (TLR) signaling pathway and total serum IgE l...


    pub_type: 杂志文章


    authors: Tewfik MA,Bossé Y,Lemire M,Hudson TJ,Vallée-Smejda S,Al-Shemari H,Laprise C,Desrosiers M

    更新日期:2009-05-01 00:00:00

  • Analysis of hsp70 gene polymorphism in allergic asthma.

    abstract:BACKGROUND:Allergic asthma is a multifactorial and probably multigenic inflammatory disease of the upper airways, and has been associated with the HLA class II alleles DR4 and DR7. Here we investigated possible associations with other polymorphic susceptibility/resistance genes located within the major histocompatibili...


    pub_type: 杂志文章


    authors: Aron Y,Busson M,Polla BS,Dusser D,Lockhart A,Swierczewski E,Favatier F

    更新日期:1999-02-01 00:00:00

  • Kiwifruit Act d 11 is the first member of the ripening-related protein family identified as an allergen.

    abstract:BACKGROUND:Kiwifruit is an important cause of food allergy. A high amount of a protein with a molecular mass compatible with that of Bet v 1 was observed in the kiwifruit extract. OBJECTIVE:To identify and characterize kirola, the 17-kDa protein of green kiwifruit (Act d 11). METHODS:Act d 11 was purified from green ...


    pub_type: 杂志文章


    authors: D'Avino R,Bernardi ML,Wallner M,Palazzo P,Camardella L,Tuppo L,Alessandri C,Breiteneder H,Ferreira F,Ciardiello MA,Mari A

    更新日期:2011-07-01 00:00:00

  • Heat treatment modifies the allergenicity of beef and bovine serum albumin.

    abstract::The effect of heat on the allergenicity of beef and bovine serum albumin was investigated among 10 toddlers skin prick test (SPT)-positive to raw and cooked beef. The meat-allergy diagnosis was confirmed during double-blind, placebo-controlled food challenge (DBPCFC) with 180 g of beef cooked for 5 min at 100 degrees ...


    pub_type: 杂志文章


    authors: Fiocchi A,Restani P,Riva E,Mirri GP,Santini I,Bernardo L,Galli CL

    更新日期:1998-08-01 00:00:00