Abstract:
:Using subtype-specific Ca-channel blockers, we have characterised the voltage-sensitive Ca2+ currents as well as neurotransmitter release from cultured mouse cerebellar granule cells. The whole cell version of the patch clamp technique was adapted to monitor the isolated Ca-channel currents. The currents were activated at potentials more positive than -40 mV and were composed of at least four pharmacological distinct components being sensitive to nifedipine (35%), omega-conotoxin GVIA (10%), and omega-agatoxin IVA (42%) corresponding to L-, N-, and P-channel-mediated currents. The insensitive fraction (13%) possibly represented R channels. High potassium-evoked release of 3H-D-aspartate was used as a model of synaptic release. These studies were performed at relatively mild stimulation conditions (30 mM K+, 0.4 mM Ca2+), and 85% of the evoked release was Ca2+ dependent as well as tetrodotoxin and Cd2+ sensitive. Nifedipine and omega-agatoxin IVA dose dependently (IC50 values of 10 nM and 0.7 nM, respectively) blocked most of the release, whereas omega-conotoxin MVIIA (IC50 = 5 nM) caused partial blockage. The results indicate that several subtypes of voltage-sensitive Ca channels are present in mouse cerebellar granule cells. Furthermore, the data suggest that L, N, and P channels act in concert in the neurotransmitter release process.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Varming T,Christopherson P,Schousboe A,Drejer Jdoi
10.1002/(sici)1097-4547(19970401)48:1<43::aid-jnr4subject
Has Abstractpub_date
1997-04-01 00:00:00pages
43-52issue
1eissn
0360-4012issn
1097-4547pii
10.1002/(SICI)1097-4547(19970401)48:1<43::AID-JNR4journal_volume
48pub_type
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