Abstract:
:Brain slices obtained from young rats were incubated with different radioactive precursors, in the presence and absence of L-cycloserine (an inhibitor of the synthesis of sphingosine) in order to explore the possibility that transport of proteolipids--and specifically of the major myelin proteolipid PLP--to the myelin membrane could be coupled to the transport of cerebrosides or sulfatides. At a concentration of 0.15 mM L-cycloserine, the incorporation of [3H] glycine into total proteins, proteolipid apoproteins (APL), PLP, and myelin basic proteins (MBP) of the total homogenate was unaffected by the presence of the inhibitor, whereas the incorporation of [3H] serine into glycosphingolipids decreased markedly. Under similar incubation conditions, the entry of labeled APL and of PLP into the myelin membranes in the presence of L-cycloserine decreased markedly (50%) in comparison to controls. Entry of MBP was not affected by the inhibitor. These results indicate that when synthesis of glycosphingolipids is inhibited by L-cycloserine, thus decreasing the availability of cerebrosides and sulfatides, the translocation of PLP to myelin is disrupted, suggesting that its transport through the oligodendroglial cell could be coupled to the transport of glycosphingolipids and, most probably, of sulfatides.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Pasquini JM,Guarna MM,Besio-Moreno MA,Iturregui MT,Oteiza PI,Soto EFdoi
10.1002/jnr.490220309subject
Has Abstractpub_date
1989-03-01 00:00:00pages
289-96issue
3eissn
0360-4012issn
1097-4547journal_volume
22pub_type
杂志文章abstract::Cultures of rat C6 rat glioma cells exhibit a diminished response to isoproterenol and forskolin after being treated with phorbol 12,13-dibutyrate (PDbU). An IC50 for PDbU of 38 +/- 5 nM and 62 +/- 8 nM was observed in the isoproterenol and forskolin response, respectively. Similarly, C6 cultures exhibited a diminishe...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250110
更新日期:1990-01-01 00:00:00
abstract::Pigment epithelium-derived factor (PEDF) is a neurotrophic protein synthesized and secreted by retinal pigment epithelial (RPE) cells in early embryogenesis and has been shown to be present in the extracellular matrix between the RPE cells and the neural retina. It induces neuronal differentiation and promotes surviva...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-09-15 00:00:00
abstract::Histology, immunohistochemistry, and Western blotting were used to characterize the changes in morphology, distribution pattern, and marker protein expression of striatal interneurons in the transition zone of striatal injury induced by 3-NP. The 3-NP treatment in rats yielded movement, motor coordination, and cogniti...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22591
更新日期:2011-05-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490260314
更新日期:1990-07-01 00:00:00
abstract::Recently we demonstrated that a member of the neuregulin-1 (NRG-1) family of growth factors is a mitogen and survival factor for olfactory ensheathing cells (OECs). OECs are specialized glial cells within the olfactory system that are believed to play a role in the continual nerve re-growth of this tissue. OECs share ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/1097-4547(20000715)61:2<172::AID-JNR8>3.0.
更新日期:2000-07-15 00:00:00
abstract::The effects of bilateral lesions of the ventral noradrenergic bundle (VNA) were studied in male rats. In contrast to data reported by others, hyperphagia and obesity were not observed following VNA lesions. Indeed, except for a depression during the first three postoperative days, food intake (FI) of the VNA lesioned ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490020305
更新日期:1976-01-01 00:00:00
abstract::Previous histochemical studies of iron in the brain demonstrated iron enrichment in oligodendrocytes, but the iron-enriched oligodendrocytes had a spatially restricted distribution that excluded major white matter tracts. In this study we used techniques designed to permeabilize tissue, combined with iron histochemist...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490260415
更新日期:1990-08-01 00:00:00
abstract::gammadelta T cells represent a small subpopulation of T cells expressing a restricted repertoire of T-cell receptors and, unlike alphabeta T cells, function more as cells of the innate immune system. These cells are found in skin and mucosal sites as well as secondary lymphoid tissues and frequently act as first line ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.22176
更新日期:2010-01-01 00:00:00
abstract::This study was undertaken in order to establish the presence of pluripotential neuroblasts in the developing chick CNS. This has been suggested by our previous observations that expression of emerging neuronal phenotypes in the chick embryo CNS is affected by exposure to neurotrophic substances (i.e., GHRH, SRIF, NGF,...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410516
更新日期:1995-08-01 00:00:00
abstract::Apoptosis is a form of cell death historically defined by morphological and biochemical changes that occur in the cell body and nucleus. However, in contrast to nonneuronal cells in which apoptosis has been most intensively studied, neurons exhibit elaborate morphologies with synaptic connections often located at site...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:
更新日期:1999-10-01 00:00:00
abstract::Factor Xa (FXa) plays a critical role in the coagulation cascade by generation of thrombin. During focal ischemia thrombin levels increase in the brain tissue and cause neural damage. This study examined the hypothesis that administration of the FXa inhibitor, apixaban, following focal ischemic stroke may have therape...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24253
更新日期:2018-08-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF-alpha) has attracted the greatest attention as a major factor in experimental autoimmune encephalomyelitis (EAE) pathogenesis. We compared rats undergoing EAE with manipulated but healthy animals by examining TNF-alpha gene expression in cells recovered from the brain. We used reverse ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960815)45:4<400::AID-JNR
更新日期:1996-08-15 00:00:00
abstract::Adrenal chromaffin cell (ACC) transplants, alone or combined with levodopa treatment, were used in attempted therapy for Parkinson's disease (PD). In a previous study, we demonstrated that levodopa caused chromaffin cell death either by necrosis or by apoptosis in cell culture. Here we report the beneficial effect of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10456
更新日期:2003-01-01 00:00:00
abstract::In this study, the responses of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) and neuronal nitric oxide synthase (nNOS) activities were quantitatively analyzed at different times in both ipsilateral and contralateral sides of trigeminal nuclei, after unilateral trigeminal muscle nerve transection, i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1235
更新日期:2001-11-01 00:00:00
abstract::We suggest that a dysregulation of energy metabolism in the brain of genetic absence epilepsy rats from Strasbourg (GAERS) could create a specific cerebral environment that would favor the expression of spike-and-wave discharges (SWD) in the thalamocortical loop, largely dependent on glutamatergic and gamma-aminobutyr...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10086
更新日期:2001-12-01 00:00:00
abstract::Living organotypic cocultures of rat thalamic and cortical explants were used to examine the effects of blocking action potential activity on the morphological development of axons in the mammalian neocortex. Studies in vivo have suggested that blocking sodium channel-dependent activity influences the growth character...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960315)43:6<707::AID-JNR
更新日期:1996-03-15 00:00:00
abstract::Neuronal degeneration has been shown to be involved in various neurological disorders. Growth/trophic factors and their receptors are known to be important for the regeneration and survival of neurons. We report here the molecular cloning of a receptor-like protein tyrosine kinase, bsk, (for brain specific kinase). Bs...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490370117
更新日期:1994-01-01 00:00:00
abstract::Molecular mechanisms which underlie the heat shock response have commonly been analyzed using tissue culture systems, with less investigation of the intact mammal. In tissue culture, a temperature elevation of 5 degrees C is required to activate mammalian heat shock transcription factor 1 (HSF1) to the DNA-binding for...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960401)44:1<52::AID-JNR7
更新日期:1996-04-01 00:00:00
abstract::The neurotrophins are important for their long-term effects on the survival and differentiation of many types of neurons during development. They also appear to protect mature neurons from injury caused by nutrient or oxygen deprivation. More recently, the neurotrophins have been implicated in such short-term processe...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-4547(19971101)50:3<355::AID-JNR
更新日期:1997-11-01 00:00:00
abstract::Transferrin is the second most abundant plasma protein and functions to transport iron. It is an essential constituent in culture media for virtually all cells. In a recent study, we reported that transferrin (Tf) is specifically located in oligodendrocytes in the rat nervous system. This investigation examines immuno...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170108
更新日期:1987-01-01 00:00:00
abstract::In offspring, an adequate maternal diet is important for neurodevelopment. One mechanism by which maternal diet impacts neurodevelopment is through its dynamic role in the development of the gut microbiota. Communication between the gut, and its associated microbiota, and the brain is facilitated by the vagus nerve, i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24605
更新日期:2021-01-01 00:00:00
abstract::The number of older people who are suffering from memory impairment is increasing among populations throughout the world. Alzheimer's disease (AD) affects about 5% of people over 65 years old. The hippocampus, a brain area critical for learning and memory, is especially vulnerable to damage in the early stages of AD. ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23319
更新日期:2014-04-01 00:00:00
abstract::In the present study, following previous experience with electrolytic lesion of the rat brain, and subsequent reduction of reactive gliosis with 7 beta-hydroxycholesterol derivatives (Bochelen et al.: Neuroscience 51:827-834, 1992), we have performed a hemisection of the spinal cord in adult rats and investigated the ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410110
更新日期:1995-05-01 00:00:00
abstract::Glial and glia-derived cells express a variety of receptors for neurotransmitters and hormones, the majority of which evoke both Ca(2+) release from intracellular stores and Ca(2+) entry across the plasma membrane. We investigated the links between histamine H(1) receptor activation, Ca(2+) release from intracellular ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21784
更新日期:2008-11-15 00:00:00
abstract::Spinocerebellar ataxia type 17 (SCA17) is a type of autosomal dominant cerebellar ataxia (ADCA) characterized by variable manifestations, including cerebellar ataxia, dementia, and psychiatric symptoms. Since the identification of a CAG repeat expansion in the TATA-box binding protein (TBP) gene in a patient with atax...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23984
更新日期:2017-08-01 00:00:00
abstract::Tissue culture media with serum generally cause excessive astrocyte proliferation in neonatal brain cultures, and often fail to support neonatal neurons. Published serum-free media for brain cultures contain sodium pyruvate, which interferes with lactate dehydrogenase (LDH) assays for cell death. We wanted to use neon...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410212
更新日期:1995-06-01 00:00:00
abstract::Among the ion channels and pumps activated by growth factor stimulation, K+ channels have been implicated in the growth and proliferation of several cancer cell lines. The role of these channels in central nervous system tumors, however, has not been described. This study used the malignant astrocytoma cell lines U87 ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1997-04-15 00:00:00
abstract::A growing body of evidence suggests that neurotrophins (NTs) play a critical role in synaptic plasticity and other activity dependent processes in the CNS. Release of these growth factors by neurons and neuroendocrine cells was recently shown to occur via the regulated secretory pathway, representing a possible mechan...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980215)51:4<463::AID-JNR
更新日期:1998-02-15 00:00:00
abstract::An aminopeptidase purified to homogeneity from cytosol of rat brain cleaved dynorphins having 5-17 residues and selected proenkephalins at the Tyr-Gly bond only to release Tyr and the desTyr fragments. The enzyme protein consisted of a single polypeptide chain of Mr 103,000 and was inhibited by puromycin, bestatin, an...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490110311
更新日期:1984-01-01 00:00:00
abstract::Given the ubiquitous nature of opioids in the treatment of pain, it is an interesting paradox that this class of medications also represents one of the least understood components of clinical pain medicine. For many years, there has been intense interest in the mechanisms of opioid activity, but this has not resulted ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24695
更新日期:2020-08-07 00:00:00