Inhibition of the synthesis of glycosphingolipids affects the translocation of proteolipid protein to the myelin membrane.

Abstract:

:Brain slices obtained from young rats were incubated with different radioactive precursors, in the presence and absence of L-cycloserine (an inhibitor of the synthesis of sphingosine) in order to explore the possibility that transport of proteolipids--and specifically of the major myelin proteolipid PLP--to the myelin membrane could be coupled to the transport of cerebrosides or sulfatides. At a concentration of 0.15 mM L-cycloserine, the incorporation of [3H] glycine into total proteins, proteolipid apoproteins (APL), PLP, and myelin basic proteins (MBP) of the total homogenate was unaffected by the presence of the inhibitor, whereas the incorporation of [3H] serine into glycosphingolipids decreased markedly. Under similar incubation conditions, the entry of labeled APL and of PLP into the myelin membranes in the presence of L-cycloserine decreased markedly (50%) in comparison to controls. Entry of MBP was not affected by the inhibitor. These results indicate that when synthesis of glycosphingolipids is inhibited by L-cycloserine, thus decreasing the availability of cerebrosides and sulfatides, the translocation of PLP to myelin is disrupted, suggesting that its transport through the oligodendroglial cell could be coupled to the transport of glycosphingolipids and, most probably, of sulfatides.

journal_name

J Neurosci Res

authors

Pasquini JM,Guarna MM,Besio-Moreno MA,Iturregui MT,Oteiza PI,Soto EF

doi

10.1002/jnr.490220309

subject

Has Abstract

pub_date

1989-03-01 00:00:00

pages

289-96

issue

3

eissn

0360-4012

issn

1097-4547

journal_volume

22

pub_type

杂志文章
  • Regulation of cAMP levels by protein kinase C in C6 rat glioma cells.

    abstract::Cultures of rat C6 rat glioma cells exhibit a diminished response to isoproterenol and forskolin after being treated with phorbol 12,13-dibutyrate (PDbU). An IC50 for PDbU of 38 +/- 5 nM and 62 +/- 8 nM was observed in the isoproterenol and forskolin response, respectively. Similarly, C6 cultures exhibited a diminishe...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490250110

    authors: Bressler JP,Tinsely P

    更新日期:1990-01-01 00:00:00

  • Pigment epithelium-derived factor protects cultured retinal neurons against hydrogen peroxide-induced cell death.

    abstract::Pigment epithelium-derived factor (PEDF) is a neurotrophic protein synthesized and secreted by retinal pigment epithelial (RPE) cells in early embryogenesis and has been shown to be present in the extracellular matrix between the RPE cells and the neural retina. It induces neuronal differentiation and promotes surviva...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:

    authors: Cao W,Tombran-Tink J,Chen W,Mrazek D,Elias R,McGinnis JF

    更新日期:1999-09-15 00:00:00

  • Preferential interneuron survival in the transition zone of 3-NP-induced striatal injury in rats.

    abstract::Histology, immunohistochemistry, and Western blotting were used to characterize the changes in morphology, distribution pattern, and marker protein expression of striatal interneurons in the transition zone of striatal injury induced by 3-NP. The 3-NP treatment in rats yielded movement, motor coordination, and cogniti...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22591

    authors: Mu S,OuYang L,Liu B,Zhu Y,Li K,Zhan M,Liu Z,Jia Y,Lei W,Reiner A

    更新日期:2011-05-01 00:00:00

  • Changes on cerebral antioxidant enzymes, peroxidation, and the glutathione system of frogs after aging and catalase inhibition.

    abstract::The five major antioxidant enzymes, glutathione, and in vivo or in vitro stimulated (Fe(++)-ascorbate) peroxidation were similar in old and young Rana perezi frogs. Long-term (2.5 months) treatment with aminotriazole strongly decreased cerebral catalase (CAT) activity and increased in vivo but not in vitro peroxidatio...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490260314

    authors: Barja de Quiroga G,Pérez-Campo R,López-Torres M

    更新日期:1990-07-01 00:00:00

  • Comparison of neuregulin-1 expression in olfactory ensheathing cells, Schwann cells and astrocytes.

    abstract::Recently we demonstrated that a member of the neuregulin-1 (NRG-1) family of growth factors is a mitogen and survival factor for olfactory ensheathing cells (OECs). OECs are specialized glial cells within the olfactory system that are believed to play a role in the continual nerve re-growth of this tissue. OECs share ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/1097-4547(20000715)61:2<172::AID-JNR8>3.0.

    authors: Thompson RJ,Roberts B,Alexander CL,Williams SK,Barnett SC

    更新日期:2000-07-15 00:00:00

  • Mesencephalic lesions resulting in normophagia, reduced weight and altered metabolism.

    abstract::The effects of bilateral lesions of the ventral noradrenergic bundle (VNA) were studied in male rats. In contrast to data reported by others, hyperphagia and obesity were not observed following VNA lesions. Indeed, except for a depression during the first three postoperative days, food intake (FI) of the VNA lesioned ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490020305

    authors: Bellinger LL,Bernardis LL,Goldman JK

    更新日期:1976-01-01 00:00:00

  • Iron-enriched oligodendrocytes: a reexamination of their spatial distribution.

    abstract::Previous histochemical studies of iron in the brain demonstrated iron enrichment in oligodendrocytes, but the iron-enriched oligodendrocytes had a spatially restricted distribution that excluded major white matter tracts. In this study we used techniques designed to permeabilize tissue, combined with iron histochemist...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490260415

    authors: LeVine SM,Macklin WB

    更新日期:1990-08-01 00:00:00

  • gammadelta T cells: the overlooked T-cell subset in demyelinating disease.

    abstract::gammadelta T cells represent a small subpopulation of T cells expressing a restricted repertoire of T-cell receptors and, unlike alphabeta T cells, function more as cells of the innate immune system. These cells are found in skin and mucosal sites as well as secondary lymphoid tissues and frequently act as first line ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.22176

    authors: Wohler JE,Smith SS,Barnum SR

    更新日期:2010-01-01 00:00:00

  • Early neuroblasts are pluripotential: colocalization of neurotransmitters and neuropeptides.

    abstract::This study was undertaken in order to establish the presence of pluripotential neuroblasts in the developing chick CNS. This has been suggested by our previous observations that expression of emerging neuronal phenotypes in the chick embryo CNS is affected by exposure to neurotrophic substances (i.e., GHRH, SRIF, NGF,...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490410516

    authors: Kentroti S,Vernadakis A

    更新日期:1995-08-01 00:00:00

  • "Apoptotic" biochemical cascades in synaptic compartments: roles in adaptive plasticity and neurodegenerative disorders.

    abstract::Apoptosis is a form of cell death historically defined by morphological and biochemical changes that occur in the cell body and nucleus. However, in contrast to nonneuronal cells in which apoptosis has been most intensively studied, neurons exhibit elaborate morphologies with synaptic connections often located at site...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:

    authors: Mattson MP,Duan W

    更新日期:1999-10-01 00:00:00

  • Apixaban decreases brain thrombin activity in a male mouse model of acute ischemic stroke.

    abstract::Factor Xa (FXa) plays a critical role in the coagulation cascade by generation of thrombin. During focal ischemia thrombin levels increase in the brain tissue and cause neural damage. This study examined the hypothesis that administration of the FXa inhibitor, apixaban, following focal ischemic stroke may have therape...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.24253

    authors: Bushi D,Chapman J,Wohl A,Stein ES,Feingold E,Tanne D

    更新日期:2018-08-01 00:00:00

  • Production of tumor necrosis factor-alpha as a result of glia-T-cell interaction correlates with the pathogenic activity of myelin basic protein-reactive T cells in experimental autoimmune encephalomyelitis.

    abstract::Tumor necrosis factor-alpha (TNF-alpha) has attracted the greatest attention as a major factor in experimental autoimmune encephalomyelitis (EAE) pathogenesis. We compared rats undergoing EAE with manipulated but healthy animals by examining TNF-alpha gene expression in cells recovered from the brain. We used reverse ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19960815)45:4<400::AID-JNR

    authors: Sun D,Hu X,Shah R,Zhang L,Coleclough C

    更新日期:1996-08-15 00:00:00

  • Fullerene C60 and ascorbic acid protect cultured chromaffin cells against levodopa toxicity.

    abstract::Adrenal chromaffin cell (ACC) transplants, alone or combined with levodopa treatment, were used in attempted therapy for Parkinson's disease (PD). In a previous study, we demonstrated that levodopa caused chromaffin cell death either by necrosis or by apoptosis in cell culture. Here we report the beneficial effect of ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10456

    authors: Corona-Morales AA,Castell A,Escobar A,Drucker-Colín R,Zhang L

    更新日期:2003-01-01 00:00:00

  • Nitric oxide synthase/nicotinamide adenine dinucleotide phosphate-diaphorase in the brainstem trigeminal nuclei after transection of the masseteric nerve in rats.

    abstract::In this study, the responses of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) and neuronal nitric oxide synthase (nNOS) activities were quantitatively analyzed at different times in both ipsilateral and contralateral sides of trigeminal nuclei, after unilateral trigeminal muscle nerve transection, i...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.1235

    authors: Varathan V,Shigenaga Y,Takemura M

    更新日期:2001-11-01 00:00:00

  • Metabolic approach of absence seizures in a genetic model of absence epilepsy, the GAERS: study of the leucine-glutamate cycle.

    abstract::We suggest that a dysregulation of energy metabolism in the brain of genetic absence epilepsy rats from Strasbourg (GAERS) could create a specific cerebral environment that would favor the expression of spike-and-wave discharges (SWD) in the thalamocortical loop, largely dependent on glutamatergic and gamma-aminobutyr...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10086

    authors: Dufour F,Nalecz KA,Nalecz MJ,Nehlig A

    更新日期:2001-12-01 00:00:00

  • Addition of tetrodotoxin alters the morphology of thalamocortical axons in organotypic cocultures.

    abstract::Living organotypic cocultures of rat thalamic and cortical explants were used to examine the effects of blocking action potential activity on the morphological development of axons in the mammalian neocortex. Studies in vivo have suggested that blocking sodium channel-dependent activity influences the growth character...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19960315)43:6<707::AID-JNR

    authors: Wilkemeyer MF,Angelides KJ

    更新日期:1996-03-15 00:00:00

  • Isolation and characterization of Bsk, a growth factor receptor-like tyrosine kinase associated with the limbic system.

    abstract::Neuronal degeneration has been shown to be involved in various neurological disorders. Growth/trophic factors and their receptors are known to be important for the regeneration and survival of neurons. We report here the molecular cloning of a receptor-like protein tyrosine kinase, bsk, (for brain specific kinase). Bs...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490370117

    authors: Zhou R,Copeland TD,Kromer LF,Schulz NT

    更新日期:1994-01-01 00:00:00

  • In vivo activation of neural heat shock transcription factor HSF1 by a physiologically relevant increase in body temperature.

    abstract::Molecular mechanisms which underlie the heat shock response have commonly been analyzed using tissue culture systems, with less investigation of the intact mammal. In tissue culture, a temperature elevation of 5 degrees C is required to activate mammalian heat shock transcription factor 1 (HSF1) to the DNA-binding for...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19960401)44:1<52::AID-JNR7

    authors: Brown IR,Rush SJ

    更新日期:1996-04-01 00:00:00

  • Actions of the neurotrophins on calcium uptake.

    abstract::The neurotrophins are important for their long-term effects on the survival and differentiation of many types of neurons during development. They also appear to protect mature neurons from injury caused by nutrient or oxygen deprivation. More recently, the neurotrophins have been implicated in such short-term processe...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-4547(19971101)50:3<355::AID-JNR

    authors: Jiang H,Guroff G

    更新日期:1997-11-01 00:00:00

  • Development of transferrin-positive oligodendrocytes in the rat central nervous system.

    abstract::Transferrin is the second most abundant plasma protein and functions to transport iron. It is an essential constituent in culture media for virtually all cells. In a recent study, we reported that transferrin (Tf) is specifically located in oligodendrocytes in the rat nervous system. This investigation examines immuno...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490170108

    authors: Connor JR,Fine RE

    更新日期:1987-01-01 00:00:00

  • The role of maternal diet on offspring gut microbiota development: A review.

    abstract::In offspring, an adequate maternal diet is important for neurodevelopment. One mechanism by which maternal diet impacts neurodevelopment is through its dynamic role in the development of the gut microbiota. Communication between the gut, and its associated microbiota, and the brain is facilitated by the vagus nerve, i...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.24605

    authors: Al Rubaye H,Adamson CC,Jadavji NM

    更新日期:2021-01-01 00:00:00

  • Novel effects of Rosa damascena extract on memory and neurogenesis in a rat model of Alzheimer's disease.

    abstract::The number of older people who are suffering from memory impairment is increasing among populations throughout the world. Alzheimer's disease (AD) affects about 5% of people over 65 years old. The hippocampus, a brain area critical for learning and memory, is especially vulnerable to damage in the early stages of AD. ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23319

    authors: Esfandiary E,Karimipour M,Mardani M,Alaei H,Ghannadian M,Kazemi M,Mohammadnejad D,Hosseini N,Esmaeili A

    更新日期:2014-04-01 00:00:00

  • Oxysterol (7 beta-hydroxycholesteryl-3-oleate) promotes serotonergic reinnervation in the lesioned rat spinal cord by reducing glial reaction.

    abstract::In the present study, following previous experience with electrolytic lesion of the rat brain, and subsequent reduction of reactive gliosis with 7 beta-hydroxycholesterol derivatives (Bochelen et al.: Neuroscience 51:827-834, 1992), we have performed a hemisection of the spinal cord in adult rats and investigated the ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490410110

    authors: Giménez y Ribotta M,Rajaofetra N,Morin-Richaud C,Alonso G,Bochelen D,Sandillon F,Legrand A,Mersel M,Privat A

    更新日期:1995-05-01 00:00:00

  • Histamine-induced Ca2+ entry in human astrocytoma U373 MG cells: evidence for involvement of store-operated channels.

    abstract::Glial and glia-derived cells express a variety of receptors for neurotransmitters and hormones, the majority of which evoke both Ca(2+) release from intracellular stores and Ca(2+) entry across the plasma membrane. We investigated the links between histamine H(1) receptor activation, Ca(2+) release from intracellular ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21784

    authors: Barajas M,Andrade A,Hernandez-Hernandez O,Felix R,Arias-Montaño JA

    更新日期:2008-11-15 00:00:00

  • Genetically modified rodent models of SCA17.

    abstract::Spinocerebellar ataxia type 17 (SCA17) is a type of autosomal dominant cerebellar ataxia (ADCA) characterized by variable manifestations, including cerebellar ataxia, dementia, and psychiatric symptoms. Since the identification of a CAG repeat expansion in the TATA-box binding protein (TBP) gene in a patient with atax...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.23984

    authors: Cui Y,Yang S,Li XJ,Li S

    更新日期:2017-08-01 00:00:00

  • A serum-free, pyruvate-free medium that supports neonatal neural/glial cultures.

    abstract::Tissue culture media with serum generally cause excessive astrocyte proliferation in neonatal brain cultures, and often fail to support neonatal neurons. Published serum-free media for brain cultures contain sodium pyruvate, which interferes with lactate dehydrogenase (LDH) assays for cell death. We wanted to use neon...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490410212

    authors: Martin FC,Wiley CA

    更新日期:1995-06-01 00:00:00

  • 4-Aminopyridine causes apoptosis and blocks an outward rectifier K+ channel in malignant astrocytoma cell lines.

    abstract::Among the ion channels and pumps activated by growth factor stimulation, K+ channels have been implicated in the growth and proliferation of several cancer cell lines. The role of these channels in central nervous system tumors, however, has not been described. This study used the malignant astrocytoma cell lines U87 ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:

    authors: Chin LS,Park CC,Zitnay KM,Sinha M,DiPatri AJ Jr,Perillán P,Simard JM

    更新日期:1997-04-15 00:00:00

  • Subcellular localization of epitope-tagged neurotrophins in neuroendocrine cells.

    abstract::A growing body of evidence suggests that neurotrophins (NTs) play a critical role in synaptic plasticity and other activity dependent processes in the CNS. Release of these growth factors by neurons and neuroendocrine cells was recently shown to occur via the regulated secretory pathway, representing a possible mechan...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19980215)51:4<463::AID-JNR

    authors: Möller JC,Krüttgen A,Heymach JV Jr,Ghori N,Shooter EM

    更新日期:1998-02-15 00:00:00

  • Formation of desTyr dynorphins 5-17 by a purified cytosolic aminopeptidase of rat brain.

    abstract::An aminopeptidase purified to homogeneity from cytosol of rat brain cleaved dynorphins having 5-17 residues and selected proenkephalins at the Tyr-Gly bond only to release Tyr and the desTyr fragments. The enzyme protein consisted of a single polypeptide chain of Mr 103,000 and was inhibited by puromycin, bestatin, an...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490110311

    authors: Berg MJ,Marks N

    更新日期:1984-01-01 00:00:00

  • Pain management with opioids in adults.

    abstract::Given the ubiquitous nature of opioids in the treatment of pain, it is an interesting paradox that this class of medications also represents one of the least understood components of clinical pain medicine. For many years, there has been intense interest in the mechanisms of opioid activity, but this has not resulted ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.24695

    authors: Montgomery LS

    更新日期:2020-08-07 00:00:00