Abstract：
:Alteration of the subcellular distribution of Mod5p-I, a tRNA modification enzyme, member of the sorting isozyme family, affects tRNA-mediated nonsense suppression. Altered suppression efficiency was used to identify MDP genes, which, when mutant, change the mitochondrial/cytosolic distribution of Mod5p-I,KR6. MDP2 is the previously identified VRP1, which encodes verprolin, required for proper organization of the actin cytoskeleton. MDP3 is identical to PAN1, which encodes a protein involved in initiation of translation and actin cytoskeleton organization. We report here the cloning and characterization of wild-type and mutant MDP1 alleles and the isolation and characterization of a multicopy suppressor of mdp1 mutations. MDP1 is identical to RSP5, which encodes ubiquitin-protein ligase, and mdp1 mutations are suppressed by high copy expression of ubiquitin. All four characterized mdp1 mutations cause missense changes located in the hect domain of Rsp5p that is highly conserved among ubiquitin-protein ligases. In addition to its well-known function in protein turnover, ubiquitination has been proposed to play roles in subcellular sorting of proteins via endocytosis and in delivery of proteins to peroxisomes, the endoplasmic reticulum and mitochondria. mdp1, as well as mdp2/vrp1 and mdp3/pan1 mutations, affect endocytosis. Further, mdp1 mutations show synthetic interactions with mdp2/vrp1 and mdp3/pan1. Identification of MDP1 as RSP5, along with our previous identification of MDP2/VRP1 and MDP3/PAN1, implicate interactions of the ubiquitin system, the actin cytoskeleton and protein synthesis in the subcellular distribution of proteins.
journal_name
Geneticsjournal_title
Geneticsauthors
Zoladek T,Tobiasz A,Vaduva G,Boguta M,Martin NC,Hopper AKsubject
Has Abstractpub_date
1997-03-01 00:00:00pages
595-603issue
3eissn
0016-6731issn
1943-2631journal_volume
145pub_type
杂志文章相关文献
GENETICS文献大全abstract：:We previously identified a transcriptional regulatory element, which we call NRE(DIT), that is required for repression of the sporulation-specific genes, DIT1 and DIT2, during vegetative growth of Saccharomyces cerevisiae. Repression through this element is dependent on the Ssn6-Tup1 corepressor. In this study, we sho...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:The rate of protein synthesis depends on both the rate of initiation of translation and the rate of elongation of the peptide chain. The rate of initiation depends on the encountering rate between ribosomes and mRNA; this rate in turn depends on the concentration of ribosomes and mRNA. Thus, patterns of codon usage th...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:Two protocols are presented that allow the selection of mutations mapping to micronuclear chromosome 5 in Tetrahymena thermophilia. One protocol involves crossing mutagenized diploid cells directly to a strain nullisomic for chromosome 5 and screening the monosomic progeny for a mutant phenotype. The second protocol f...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:Vertebrate retrotransposons have been used extensively for phylogenetic analyses and studies of molecular evolution. Information can be obtained from specific inserts either by comparing sequence differences that have accumulated over time in orthologous copies of that insert or by determining the presence or absence ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：:Fossil evidence links human ancestry with populations that evolved from modern gracile morphology in Africa 130,000-160,000 years ago. Yet fossils alone do not provide clear answers to the question of whether the ancestors of all modern Homo sapiens comprised a single African population or an amalgamation of distinct ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.041095
更新日期：2005-08-01 00:00:00
abstract：:The replication of the rDNA complement of only one nucleolus organizer region during polytene chromosome formation (replicative dominance) was initially observed in Drosophila melanogaster. Here we demonstrate replicative dominance in Drosophila simulans and D. melanogaster/D. simulans interspecific hybrids. A second ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:DNA sequence variation in a 1410-bp region including the Cu,Zn Sod locus was examined in 41 homozygous lines of Drosophila melanogaster. Fourteen lines were from Barcelona, Spain, 25 were from California populations and the other two were from laboratory stocks. Two common electromorphs, SODS and SODF, are segregating...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:A mutant strain of Chlamydomonas reinhardi which is UV sensitive as a result of a single-gene chromosomal mutation has also been found to exhibit reduced recombination. In crosses homozygous for the mutant allele, a reduction in recombination frequency was demonstrated in two different linkage groups and in three diff...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1972-06-01 00:00:00
abstract：:Differential gene expression across cell types underlies development and cell physiology in multicellular organisms. Caenorhabditis elegans is a powerful, extensively used model to address these biological questions. A remaining bottleneck relates to the difficulty to obtain comprehensive tissue-specific gene transcri...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303774
更新日期：2020-12-01 00:00:00
abstract：:Levels of genetic variation and linkage disequilibrium (LD) are critical factors in association mapping methods as well as in identification of loci that have been targets of selection. Maize, an outcrosser, has a high level of sequence variation and a limited extent of LD. Sorghum, a closely related but largely self-...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.167.1.471
更新日期：2004-05-01 00:00:00
abstract：:A canon of population genetics concerns the properties of FST, a descriptor of spatial genetic structure. Interest for FST arose from Wright's early insights linking FST to dispersal parameters as well as to his concept of effective population size (e.g., Wright 1938, 1951). Although there is continued interest in thi...
journal_title：Genetics
pub_type： 评论,杂志文章
doi：10.1534/genetics.113.152132
更新日期：2013-07-01 00:00:00
abstract：:Self-incompatibility (SI) is a genetic system found in some hermaphrodite plants. Recognition of pollen by pistils expressing cognate specificities at two linked genes leads to rejection of self pollen and pollen from close relatives, i.e., to avoidance of self-fertilization and inbred matings, and thus increased outc...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.127399
更新日期：2011-07-01 00:00:00
abstract：:The F(1) hybrids of Nicotiana suaveolens (subgenus Petunioides, 2n = 32) and N. glutinosa (subgenus Tabacum, 2n = 24), were examined during their development, from seedlings to mature plants. It was observed that in the hybrids, there was a progressive change of dominant N. glutinosa morphological characteristics towa...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-04-01 00:00:00
abstract：:Mapping of expression quantitative trait loci (eQTL) is a powerful means for elucidating the genetic architecture of gene regulation. Yet, eQTL mapping has not been applied toward investigating the regulation architecture of genes involved in the process of population divergence, ultimately leading to speciation event...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.086306
更新日期：2008-08-01 00:00:00
abstract：:The determination of segment identity in Drosophila melanogaster appears to be controlled by a small number of genes. In order to identity new components in the process, we have systematically screened the autosomal complement for loci that show a dosage-dependent interaction with mutations in previously characterized...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:The effect of nonhomologous DNA sequences at one or both sides of short genetic intervals on recombination within that interval was investigated, using an interplasmidic recombination system in Escherichia coli K-12. The recombining plasmids were derivatives of pBR322 and pACYC184, which share a 1330-nucleotide sequen...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:Knowing the amount of DNA polymorphism is essential to understand the mechanism of maintaining DNA polymorphism in a natural population. The amount of DNA polymorphism can be measured by the average number of nucleotide differences per site (pi), the proportion of segregating (polymorphic) site (s) and the minimum num...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
abstract：:Genotype by environment interactions (GEI) play a major part in shaping the genetic architecture of quantitative traits and are confounding factors in genetic studies, for example, in attempts to associate genetic variation with disease susceptibility. It is generally not known what proportion of phenotypic variation ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.086769
更新日期：2008-06-01 00:00:00
abstract：:Accumulation of RNA was measured in adult males of two genotypes: car bb/Ybb- and car bb/YbbSuVar-5. The two genotypes have similar amounts of rDNA, which is reduced in comparison to wild type (CLARK, STRAUSBAUGH and KIEFER 1977). Although genotypically bobbed, car bb/YbbSuVar-5 flies have a wild-type phenotype; car b...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
abstract：:Knowledge of multigenic family organization should provide insight into their mode of evolution. Accordingly, we characterized the 5S ribosomal gene family in the Drosophila melanogaster strain ry506. The 5S genes in this strain display a striking HindIII restriction difference compared to the "standard" D. melanogast...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:In a previous study, a mitochondrial mutant expressing a specific enzymatic deficiency in co-enzyme QH2-cytochrome c reductase was described (TZAGO-LOFF, FOURY and AKAI 1976). Analysis of the mitochondrially translated proteins revealed the absence in the mutant of the mitochondrial product corresponding to cytochrome...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：:Mutants arising spontaneously from sporulated cultures of certain strains of yeast, Saccharomyces cerevisiae, contained deletions of the CYC1 gene which controls the primary structure of iso-1-cytochrome c. At least 60 different kinds of deletions were uncovered among the 104 deletions examined and these ranged in len...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1975-09-01 00:00:00
abstract：:The homeotic genes of the Drosophila bithorax complex are controlled by a large cis-regulatory region that ensures their segmentally restricted pattern of expression. A deletion that removes the Frontabdominal-7 cis-regulatory region (Fab-7') dominantly transforms parasegment 11 into parasegment 12. Previous studies s...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:Inactivation of the maternal or paternal X chromosome in a mammalian embryonic XX cell is believed to involve random choice between the two X's. We propose two alternative models. One suggests that choice is not random, while the other is consistent with random choice, but not one between two X's. ...
journal_title：Genetics
pub_type： 评论,杂志文章
doi：10.1534/genetics.104.029819
更新日期：2004-07-01 00:00:00
abstract：:The Saccharomyces cerevisiae PHO85 gene encodes a nonessential cyclin-dependent kinase that associates with 10 cyclin subunits. To survey the functions provided by Pho85, we identified mutants that require PHO85 for viability. We identified mutations that define seven Pho Eighty-Five Requiring or Efr loci, six of whic...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Segregation data were obtained for 1260 potato linkage group I-specific AFLP loci from a heterozygous diploid potato population. Analytical tools that identified potential typing errors and/or inconsistencies in the data and that assembled cosegregating markers into bins were applied. Bins contain multiple-marker data...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：:Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure Delta-9-tetrahydrocannabinol (THC) chemotypes. All the plants belonging to the F(1)'s were analyzed by gas chromatography for cannabinoid composition and constantly found to have a mixed CBD-THC chemotype. Ten individual ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:In Drosophila melanogaster, the endogenous retrovirus gypsy is repressed by the functional alleles (restrictive) of an as-yet-uncloned heterochromatic gene called flamenco. Using gypsy-lacZ transcriptional fusions, we show here that this repression takes place not only in the follicle cells of restrictive ovaries, as ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.166.3.1313
更新日期：2004-03-01 00:00:00
abstract：:We describe a fast hierarchical Bayesian method for mapping quantitative trait loci by haplotype-based association, applicable when haplotypes are not observed directly but are inferred from multiple marker genotypes. The method avoids the use of a Monte Carlo Markov chain by employing priors for which the likelihood ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.113183
更新日期：2010-03-01 00:00:00
abstract：:A spontaneous lethal mutation rate approximately twenty to thirty times greater than normal has been discovered in second and third chromosomes derived from an irradiated isogenic line and paired with marked inversion chromosomes. Mutations resulting in reductions of viability of varying magnitude short of complete le...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-09-01 00:00:00
