当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF CANCER期刊下所有文献 > Glucocorticoid-receptor-gene defects and resistance to glucocorticoid-induced apoptosis in human leukemic cell lines.

Glucocorticoid-receptor-gene defects and resistance to glucocorticoid-induced apoptosis in human leukemic cell lines.

Abstract：

:The application of glucocorticoids (GC3) in human leukemia is based on apoptosis induction but is often hampered by GC resistance. To delineate resistance mechanisms, we examined 5 GC-resistant leukemic cell lines, termed CEM-C7.R1-R5, isolated from the GC-sensitive human acute-T-cell-leukemic line, CCRF-CEM-C7, by selection in GC-containing medium. GC resistance was ascertained by analyzing cell-cycle progression, proliferation, and apoptosis. Radioreceptor assays revealed absence of ligand-binding activity in all clones, suggesting that defects in GC-receptor(GR) expression cause GC resistance. Analyses of the GR gene revealed that all but one (CEM-C7.R5) of the clones were heterozygous for the previously described L753F mutation. CEM-C7.R5 was either hemi- or homozygous for the L753F mutation and, hence, lacked a functional GR. Sequencing of the allele not carrying the L753F mutation of the other GC-resistant sub-lines revealed additional mutations in the GR gene in 3 cases: CEM-C7.R1 and R2 had a base-pair deletion in exon 9 (deltaT740) that resulted in a reading-frame shift and a pre-terminal in-frame stop. Translation of this mutant mRNA would produce a protein lacking 32 amino acids and expressing 4 altered residues at its new C terminus. CEM-C7.R3 harbored a non-sense mutation (Q710X) in exon 8, and its mRNA would be translated into a protein lacking 67 residues. Only CEM-C7.R4 cells were devoid of mutations in the coding region of the L753F negative allele. These data suggest that, in the CCRF-CEM acute-lymphatic-leukemia model, mutations in the GR-gene coding region represent one, but not the only, cause of GC resistance.

journal_name

Int J Cancer

journal_title

International journal of cancer

authors

Hala M,Hartmann BL,Böck G,Geley S,Kofler R

doi

10.1002/(SICI)1097-0215(19961127)68:5<663::AID-IJC

subject

Has Abstract

pub_date

1996-11-27 00:00:00

pages

663-8

issue

5

eissn

0020-7136

issn

1097-0215

pii

10.1002/(SICI)1097-0215(19961127)68:5<663::AID-IJC

journal_volume

68

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF CANCER文献大全
  • Increased phosphorylation of nuclear phosphoproteins in human lung-cancer cells resistant to cis-diamminedichloroplatinum (II).

    abstract：:A novel non-phorbol-ester-like tumor promoter, okadaic acid (OA) has been shown to be an inhibitor of protein phosphatase I and IIA and, thus, to cause an "apparent activation" of protein kinase C (PKC). We previously showed that cis-diamminedichloroplatinum(II) (CDDP)-resistant cells, PC-9/CDDP, were cross-resistant ...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910500319

    authors： Nishio K,Sugimoto Y,Kasahara K,Fujiwara Y,Nishiwaki S,Fujiki H,Ohata M,Saijo N

    更新日期：1992-02-01 00:00:00

  • Serum CA 50 as a tumor marker in pancreatic cancer: a comparison with CA 19-9.

    abstract：:CA 50 is a new tumor marker based on a monoclonal antibody (MAb) against a human colorectal carcinoma cell line. The CA 50 antigen is similar, but not identical, to the tumor marker CA 19-9. The serum concentrations of CA 50 were measured by an immunoradiometric assay (CA 50 IRMA) in 95 patients with pancreatic cancer...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910390412

    authors： Haglund C,Kuusela P,Jalanko H,Roberts PJ

    更新日期：1987-04-15 00:00:00

  • Dietary agent indole-3-carbinol protects female rats against the hepatotoxicity of the antitumor drug ET-743 (trabectidin) without compromising efficacy in a rat mammary carcinoma.

    abstract：:ET-743, an experimental antitumor drug with promising activity in sarcoma, breast and ovarian carcinoma, is currently under phase 2 clinical evaluation. It is hepatotoxic in animals and patients. We tested the hypothesis that indole-3-carbinol (I3C), the hydrolysis product of glucosinolates occurring in cruciferous ve...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.20356

    authors： Donald S,Verschoyle RD,Greaves P,Colombo T,Zucchetti M,Falcioni C,Zaffaroni M,D'Incalci M,Manson MM,Jimeno J,Steward WP,Gescher AJ

    更新日期：2004-10-10 00:00:00

  • Canine testicular tumors: epidemiologic features of 410 dogs.

    abstract：:Histologically confirmed testicular tumors were diagnosed in 410 dogs from 12 North American veterinary university hospitals and clinics. Three tumor-cell types, Sertoli cell tumor, interstitial cell tumor and seminoma, were about equally represented. Several breeds were identified with high risk for different testicu...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910180413

    authors： Hayes HM Jr,Pendergrass TW

    更新日期：1976-10-15 00:00:00

  • Exclusion of BRAFV599E as a melanoma susceptibility mutation.

    abstract：:Very recently, BRAF mutations were found in about 2/3 of malignant melanomas and at lower frequencies in other human cancers. The BRAF gene codes for a protein in the mitogen-activated protein kinase (MAPK) pathway. All mutations identified to date are within the kinase domain, with a single missense mutation (V599E) ...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.11199

    authors： Meyer P,Klaes R,Schmitt C,Boettger MB,Garbe C

    更新日期：2003-08-10 00:00:00

  • MCC inhibits beta-catenin transcriptional activity by sequestering DBC1 in the cytoplasm.

    abstract：:The mutated in colorectal cancer (MCC) is a multifunctional gene showing loss of expression in colorectal and liver cancers. MCC mutations can drive colon carcinogenesis in the mouse and in vitro experiments suggest that loss of MCC function promotes cancer through several important cellular pathways. In particular, t...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.28967

    authors： Pangon L,Mladenova D,Watkins L,Van Kralingen C,Currey N,Al-Sohaily S,Lecine P,Borg JP,Kohonen-Corish MR

    更新日期：2015-01-01 00:00:00

  • Loss of specific natural cell-mediated cytotoxicity with absorption of natural antibodies from serum.

    abstract：:When effector cells from most individuals are tested directly against a number of target cells for cell-mediated cytotoxicity, most of the reactivity is non-selective. This apparent non-selectiveness is a result of specific activity against multiple antigens on target cells by each effector suspension. The specific re...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910190603

    authors： Akira D,Takasugi M

    更新日期：1977-06-15 00:00:00

  • Cell death induced by N-(4-hydroxyphenyl)retinamide in human epidermal keratinocytes is modulated by TGF-beta and diminishes during the progression of squamous cell carcinoma.

    abstract：:It has been demonstrated that the chemopreventive agent N-(4-hydroxyphenyl)retinamide (4-HPR) induces apoptotic cell death, but recent data has suggested that late stage/recurrent tumours lose their response to 4-HPR-induced cell death by mechanisms that are unknown. Our study investigated the ability of 4-HPR to indu...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.22263

    authors： Davies M,Paterson IC,Ganapathy A,Prime SS

    更新日期：2006-12-15 00:00:00

  • Mutation analysis of the MYH gene in an Australian series of colorectal polyposis patients with or without germline APC mutations.

    abstract：:The MYH gene has recently been shown to be associated with a recessive form of colorectal adenomatous polyposis. Two common mutations in the MYH gene have been identified that lend themselves to rapid screening. We have examined a series of 302 individuals comprising 120 control subjects, 120 patients diagnosed with a...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.20983

    authors： Kairupan CF,Meldrum CJ,Crooks R,Milward EA,Spigelman AD,Burgess B,Groombridge C,Kirk J,Tucker K,Ward R,Williams R,Scott RJ

    更新日期：2005-08-10 00:00:00

  • Over-expression of p53 nuclear oncoprotein in transitional-cell bladder cancer and its prognostic value.

    abstract：:Two hundred and twelve archival bladder-cancer biopsy specimens were analyzed immunohistochemically analysis were correlated to established histological and quantitative prognostic factors and survival of patients during a mean follow-up period of more than 10 years. Twenty-nine percent of tumours were positive for p5...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910530304

    authors： Lipponen PK

    更新日期：1993-02-01 00:00:00

  • Expression of stress protein gp96, a tumor rejection antigen, in human colorectal cancer.

    abstract：:Preparations of stress protein gp96 from tumor cells are active as tumor vaccines by eliciting immune responses against mixtures of individual tumor peptide antigens which are complexed to gp96. Due to the individual antigenicity of tumors, a vaccine consisting of tumor-derived gp96 has to be prepared individually for...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/(sici)1097-0215(20000515)86:4<489::aid-ijc

    authors： Heike M,Frenzel C,Meier D,Galle PR

    更新日期：2000-05-15 00:00:00

  • Plasmacytoma induction in radiochimeras.

    abstract：:We have induced plasmacytomas (MPC) in BALB/c radiochimeras (RCh) repopulated with syngeneic hemopoietic cells carrying distinctive chromosomal markers. A group of 38 RCh that received 0.5 ml pristane, followed by Abelson virus infection 2-3 weeks later, developed 7 tumors (18.5%) of donor origin after a relatively sh...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910440225

    authors： Silva S,Babonits M,Wiener F,Klein G

    更新日期：1989-08-15 00:00:00

  • Cell-mediated immune reactivity of Sinclair melanoma-bearing swine to 3 M KCl extracts of swine and human melanoma.

    abstract：:Leukocyte migration inhibition (LMI) assays were performed using 3 M KCI extracts to detect cell-mediated immune reactions against tumor-associated antigens (TAA) of swine melanoma. Positive LMI reactivity was 70% or greater in melanoma-bearing swine compared to 10% reactivity or less in normal swine tested with extra...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910310516

    authors： Hook RR Jr,Hamby CV,Millikan LE,Berkelhammer J,Stills HF Jr,Oxenhandler RW

    更新日期：1983-05-15 00:00:00

  • Role of XIAP in the malignant phenotype of transitional cell cancer (TCC) and therapeutic activity of XIAP antisense oligonucleotides against multidrug-resistant TCC in vitro.

    abstract：:XIAP directly inhibits executor caspases, making it the most downstream antiapoptotic molecule. Here, we examined the expression and function of XIAP in normal urothelium and TCC. We also examined the therapeutic effect of xiap AS PODN on the cell cycle and apoptosis of multidrug-resistant T24 bladder cancer cells. XI...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.10776

    authors： Bilim V,Kasahara T,Hara N,Takahashi K,Tomita Y

    更新日期：2003-01-01 00:00:00

  • Family history of urogenital cancers in patients with bladder, renal cell and prostate cancers.

    abstract：:Family history of urogenital cancers has been associated with an increased risk of cancer at the same sites. To evaluate the risks of bladder, prostate and renal cell neoplasms for family history of urogenital cancers in first-degree relatives, we analysed data from a case-control study conducted in northern Italy bet...

    journal_title：International journal of cancer

    pub_type： 杂志文章,多中心研究

    doi：10.1002/ijc.23037

    authors： Randi G,Pelucchi C,Negri E,Talamini R,Galeone C,Franceschi S,La Vecchia C

    更新日期：2007-12-15 00:00:00

  • Relationship of SNCG, S100A4, S100A9 and LCN2 gene expression and DNA methylation in bladder cancer.

    abstract：:Microarray analysis of paired cultures of normal and cancerous urothelial cells revealed differences in cytokeratin and adhesion gene expression. Normal cells expressed autocrine growth factor genes more strongly whereas carcinoma cells were distinguished by concomitant expression of urothelial and epidermal different...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.23893

    authors： Dokun OY,Florl AR,Seifert HH,Wolff I,Schulz WA

    更新日期：2008-12-15 00:00:00

  • Identification of 20 genes aberrantly methylated in human breast cancers.

    abstract：:Aberrant methylation of CpG islands (CGI) not only plays a role in gene silencing, but is also a potential cancer biomarker. To identify more CGI aberrantly methylated in human breast cancers, we carried out a genome-wide search for aberrant methylation, using methylation-sensitive-representational difference analysis...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.21054

    authors： Miyamoto K,Fukutomi T,Akashi-Tanaka S,Hasegawa T,Asahara T,Sugimura T,Ushijima T

    更新日期：2005-09-01 00:00:00

  • Accuracy of human papillomavirus testing in primary screening of cervical neoplasia: results from a multicenter study in India.

    abstract：:The knowledge that cervical neoplasia are caused by human papillomavirus (HPV) infection has led to the evaluation of its role in screening. We evaluated the accuracy of HPV testing by Hybrid capture II (HC II) method in detecting cervical intraepithelial neoplasia grade 2 and 3 (CIN 2 and 3) lesions in 4 cross-sectio...

    journal_title：International journal of cancer

    pub_type： 杂志文章,多中心研究

    doi：10.1002/ijc.20396

    authors： Sankaranarayanan R,Chatterji R,Shastri SS,Wesley RS,Basu P,Mahe C,Muwonge R,Seigneurin D,Somanathan T,Roy C,Kelkar R,Chinoy R,Dinshaw K,Mandal R,Amin G,Goswami S,Pal S,Patil S,Dhakad N,Frappart L,Fontaniere B

    更新日期：2004-11-01 00:00:00

  • RNAi-mediated downregulation of urokinase plasminogen activator receptor and matrix metalloprotease-9 in human breast cancer cells results in decreased tumor invasion, angiogenesis and growth.

    abstract：:The serine protease urokinase-type plasminogen activator (uPA) plays a significant role in tumor cell invasion and metastasis when bound to its specific receptor, uPAR (also known as CD87). In addition to the uPA-uPAR system, matrix metalloproteinases (MMPs) are involved in tumor cell invasion and metastasis. In this ...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.22962

    authors： Kunigal S,Lakka SS,Gondi CS,Estes N,Rao JS

    更新日期：2007-11-15 00:00:00

  • Melphalan availability in hypoxia-inducible factor-1alpha+/+ and factor-1alpha-/- tumors is independent of tumor vessel density and correlates with melphalan erythrocyte transport.

    abstract：:Many tumors are impervious to anticancer agents. Resistance due to lack of cytotoxic penetration into the tumor is often overlooked but can play a significant role in undermining therapy. Insufficient and irregular vascularization of tumors is a possible barrier to drug delivery, but there are others, e.g., impaired v...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.10391

    authors： Wildiers H,Guetens G,de Boeck G,Landuyt W,Verbeken E,Highley M,de Bruijn EA,van Oosterom AT

    更新日期：2002-06-01 00:00:00

  • The gene for the cyclin-dependent-kinase-4 inhibitor, CDKN2A, is preferentially deleted in malignant mesothelioma.

    abstract：:Cytogenetic deletions of the short arm of chromosome 9, 9p, have been detected in cell lines of malignant mesothelioma as well as in tumor material. Many tumor types carry deletions of chromosome 9 or more specifically of 9p21. The tumor-suppressor genes, CDKN2A and CDKN2B, each of which encodes a structurally and fun...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/(sici)1097-0215(19980209)75:4<649::aid-ijc

    authors： Prins JB,Williamson KA,Kamp MM,Van Hezik EJ,Van der Kwast TH,Hagemeijer A,Versnel MA

    更新日期：1998-02-09 00:00:00

  • Toward understanding nonparticipation in sigmoidoscopy screening for colorectal cancer.

    abstract：:Understanding the reasons for nonparticipation in cancer screening may give clues about how to improve compliance. However, limited cooperation has hampered research on nonparticipant profiles. We took advantage of Sweden's comprehensive demographic and health care registers to investigate characteristics of all parti...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.23208

    authors： Blom J,Yin L,Lidén A,Dolk A,Jeppsson B,Påhlman L,Holmberg L,Nyrén O

    更新日期：2008-04-01 00:00:00

  • Complement-dependent cytotoxic antibodies in the course of cervical carcinoma.

    abstract：:Complement-dependent cytotoxic antibodies to three cervical carcinoma cell lines (Me-180, SW-732 and HeLa) and to Herpes simplex virus type 2 (HSV-2)-infected cells were determined in a long-term study of women with cervical carcinoma. Cytolysis of surface antigens differed significantly between the cervical carcinoma...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910200508

    authors： Christenson B

    更新日期：1977-11-15 00:00:00

  • Reactive oxygen species-mediated regulation of eNOS and iNOS expression in multicellular prostate tumor spheroids.

    abstract：:Nitric oxide (NO) generated by either endothelial nitric oxide synthase (eNOS) or inducible nitric oxide synthase (iNOS) may be involved in prostate tumorigenesis through the inhibition of reactive oxygen species (ROS)-induced apoptosis. Multicellular DU-145 prostate tumor spheroids endogenously generated NO that para...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.10928

    authors： Wartenberg M,Schallenberg M,Hescheler J,Sauer H

    更新日期：2003-04-10 00:00:00

  • Heterogeneity of HLA and EBER expression in Epstein-Barr virus-associated nasopharyngeal carcinoma.

    abstract：:Nasopharyngeal carcinoma (NPC) is an aggressive tumour of multifactorial aetiology that, although rare in most parts of the world, poses a significant mortality problem in its high incidence area of Southern China. Improved therapies are an urgent requirement and, towards this end, immunotherapeutic methods are being ...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/1097-0215(20001215)88:6<949::aid-ijc18>3.0

    authors： Yao Y,Minter HA,Chen X,Reynolds GM,Bromley M,Arrand JR

    更新日期：2000-12-15 00:00:00

  • Co-expression of heparin-binding EGF-like growth factor and related peptides in human gastric carcinoma.

    abstract：:Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) is a member of the EGF family of polypeptide growth factors, which includes EGF, transforming growth factor alpha(TGF-alpha), amphiregulin (AR) and betacellulin (BTC). To assess the potential role of HB-EGF in human gastric carcinomas, the expre...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/(SICI)1097-0215(19960503)66:3<315::AID-IJC

    authors： Naef M,Yokoyama M,Friess H,Büchler MW,Korc M

    更新日期：1996-05-03 00:00:00

  • A sensitive test for the detection of specific DSB repair defects in primary cells from breast cancer specimens.

    abstract：:Increasing evidence indicates that breast cancer pathogenesis is linked with DNA double-strand break (DSB) repair dysfunction. This conclusion is based on advances in the study of functions of breast cancer susceptibility genes such as BRCA1 and BRCA2, on the identification of breast cancer-associated changes regardin...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.23551

    authors： Keimling M,Kaur J,Bagadi SA,Kreienberg R,Wiesmüller L,Ralhan R

    更新日期：2008-08-01 00:00:00

  • Cervical carcinoma: detection of herpes simplex virus RNA in cells undergoing neoplastic change.

    abstract：:3H-HSV-2 DNA has been hybridized in situ to frozen sectioned tissue from human cervical biopsies. RNA complementary to the virus-specific probe was detected in cells undergoing pre-malignant changes, but not in the cells of the fully developed squamous-cell cancer. ...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910250102

    authors： McDougall JK,Galloway DA,Fenoglio CM

    更新日期：1980-01-15 00:00:00

  • Effects of DL-alpha-difluoromethylornithine on the growth and metastasis of B16 melanoma in vivo.

    abstract：:The effects of DL-alpha-difluoromethylornithine (DFMO), a specific irreversible inhibitor of ornithine decarboxylase, on the growth of experimental mouse B16-F10 melanoma cells were investigated. DFMO (3%) in drinking water was administered to B16-F10 melanoma-bearing mice. At 24 days, B16-F10 melanomas in DFMO-fed mi...

    journal_title：International journal of cancer

    pub_type： 杂志文章

    doi：10.1002/ijc.2910390220

    authors： Kubota S,Ohsawa N,Takaku F

    更新日期：1987-02-15 00:00:00

  • Loss of expression of the tumour suppressor gene AIMP3 predicts survival following radiotherapy in muscle-invasive bladder cancer.

    abstract：:The aim of this study was to test the utility of AIMP3, an upstream regulator of DNA damage response following genotoxic stress, as a clinical biomarker in muscle-invasive bladder cancer (MIBC). AIMP3 was identified from a meta-analysis of a global gene-expression dataset. AIMP3 protein expression was determined by im...

    journal_title：International journal of cancer

    pub_type： 杂志文章,多中心研究

    doi：10.1002/ijc.29022

    authors： Gurung PM,Veerakumarasivam A,Williamson M,Counsell N,Douglas J,Tan WS,Feber A,Crabb SJ,Short SC,Freeman A,Powles T,Hoskin PJ,West CM,Kelly JD

    更新日期：2015-02-01 00:00:00