Co-expression of heparin-binding EGF-like growth factor and related peptides in human gastric carcinoma.


:Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) is a member of the EGF family of polypeptide growth factors, which includes EGF, transforming growth factor alpha(TGF-alpha), amphiregulin (AR) and betacellulin (BTC). To assess the potential role of HB-EGF in human gastric carcinomas, the expression of HB-EGF and EGF receptor (EGF-R) was examined in normal and cancerous gastric tissues and cultured gastric cancer cell lines. By Northern blot analysis, there was a 4.7-fold increase in HB-EGF mRNA levels in human gastric cancers compared with normal gastric tissues. There was a concomitant 3.9-fold increase in EGF-R mRNA levels in these cancers. Immunostaining revealed co-localization in 72% of the cancer cells of HB-EGF and EGF-R. AR and BTC moieties were not evident by Northern blot analysis. However, using PCR, both AR and BTC mRNA species were demonstrated in normal and cancerous gastric tissues. By Northern blot analysis, HB-EGF, TGF-alpha, AR, BTC and EGF-R mRNA moieties were co-expressed in KATO III and NCI-N87 gastric cancer cell lines. Furthermore, HB-EGF, EGF and TGF-alpha enhanced the growth of both cell lines in a dose-dependent manner. Our findings suggest that HB-EGF is relatively abundant in human gastric cancers and that co-expression of the EGF ligand family may lead to excessive activation of EGF-R in this disorder.


Int J Cancer


Naef M,Yokoyama M,Friess H,Büchler MW,Korc M




Has Abstract


1996-05-03 00:00:00














  • Selective induction of endothelial cell tissue factor in the presence of a tumour-derived mediator: a potential mechanism of flavone acetic acid action in tumour vasculature.

    abstract::Flavone acetic acid (FAA) is a potentially useful anti-tumour agent which has been reported to induce changes in tumour vasculature, in particular loss of bloodflow. This led us to examine whether endothelium could be a cellular target of FAA action, with resultant modulation of cell-surface coagulant properties leadi...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Murray JC,Clauss M,Denekamp J,Stern D

    更新日期:1991-09-09 00:00:00

  • Expression and functional activity of fibroblast growth factors and their receptors in human pancreatic cancer.

    abstract::We have analysed expression of the first 7 members of the family of heparin-binding fibroblast growth factor (FGFs) and their 4 high-affinity receptors (FGFRs) in human pancreatic carcinoma cell lines, both at the mRNA and protein levels. In cell lines expressing FGFRs, 2 typical patterns were observed: (i) expression...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Leung HY,Gullick WJ,Lemoine NR

    更新日期:1994-12-01 00:00:00

  • Defining a role for Sonic hedgehog pathway activation in desmoplastic medulloblastoma by identifying GLI1 target genes.

    abstract::A subgroup of medulloblastomas shows constitutive activation of the Sonic hedgehog pathway with expression of GLI1. We identified the subset of GLI1 transforming target genes specifically expressed in medulloblastomas by comparing GLI1 targets in RK3E cells transformed by GLI1 with the gene expression profile of Sonic...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yoon JW,Gilbertson R,Iannaccone S,Iannaccone P,Walterhouse D

    更新日期:2009-01-01 00:00:00

  • Loss of EphA2 receptor tyrosine kinase reduces ApcMin/+ tumorigenesis.

    abstract::The Eph receptor A2 (EphA2) is overexpressed in a range of human epithelial cancers, a phenotype that is associated with cancer cell proliferation, progression and angiogenesis. Mouse models of mammary neoplasia have confirmed the role of EphA2 as mice carrying a knockout allele of EphA2 were resistant to breast cance...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bogan C,Chen J,O'Sullivan MG,Cormier RT

    更新日期:2009-03-15 00:00:00

  • Long noncoding RNA CCAL transferred from fibroblasts by exosomes promotes chemoresistance of colorectal cancer cells.

    abstract::Long noncoding RNAs (lncRNAs) are involved in the pathology of colorectal cancer (CRC). Current efforts to eradicate CRC predominantly focused on targeting the proliferation of rapidly growing cancer epithelial cells. This is largely ineffective with resistance arising in most tumors after exposure to chemotherapy. De...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Deng X,Ruan H,Zhang X,Xu X,Zhu Y,Peng H,Zhang X,Kong F,Guan M

    更新日期:2020-03-15 00:00:00

  • Fetal myofibroblast-like cells isolated from post-radiation fibrosis in human breast cancer.

    abstract::Cells were isolated from post-radiation fibrosis biopsies of patients with recurrent breast carcinoma. These cells were identified as fibroblasts and compared with fibroblasts from normal breast tissues for their proliferative activities, chromosome number and for the presence of various components of the extracellula...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Brouty-Boyé D,Raux H,Azzarone B,Tamboise A,Tamboise E,Béranger S,Magnien V,Pihan I,Zardi L,Israël L

    更新日期:1991-03-12 00:00:00

  • Regional variations in cancer survival: Impact of tumour stage, socioeconomic status, comorbidity and type of treatment in Norway.

    abstract::Cancer survival varies by place of residence, but it remains uncertain whether this reflects differences in tumour, patient and treatment characteristics (including tumour stage, indicators of socioeconomic status (SES), comorbidity and information on received surgery and radiotherapy) or possibly regional differences...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Skyrud KD,Bray F,Eriksen MT,Nilssen Y,Møller B

    更新日期:2016-05-01 00:00:00

  • KLF6, a putative tumor suppressor gene, is mutated in astrocytic gliomas.

    abstract::Gliomas are the most common tumors of the central nervous system and have a grave prognosis. Deletion of chromosome 10p15 is one of the most common chromosomal alterations in gliomas. Recently, a candidate tumor suppressor gene, KLF6, which is mapped to chromosome 10p, was found to be frequently mutated in prostate ca...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Jeng YM,Hsu HC

    更新日期:2003-07-10 00:00:00

  • Expression of protectin (CD59) in human melanoma and its functional role in cell- and complement-mediated cytotoxicity.

    abstract::Immunohistochemical and/or indirect immunofluorescence analysis with monoclonal antibody (MAb) H19 demonstrated the expression of protectin (CD59) in 54 surgically removed metastatic melanoma lesions and on 8 out of 12 melanoma cell lines. CD59 expression had a low degree of intra- and intertumor heterogeneity. SDS-PA...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Brasoveanu LI,Altomonte M,Gloghini A,Fonsatti E,Coral S,Gasparollo A,Montagner R,Cattarossi I,Simonelli C,Cattelan A

    更新日期:1995-05-16 00:00:00

  • Scatter factor modulates the metastatic phenotype of the EMT6 mouse mammary tumor.

    abstract::EMT6 is a transplantable mouse mammary tumor cell line that has been utilized widely as a model system to study the effects of various treatments on local tumor growth and pulmonary metastasis. In this study, we examined the cellular mechanisms by which scatter factor (SF), a fibroblast-derived cytokine that stimulate...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rosen EM,Knesel J,Goldberg ID,Jin L,Bhargava M,Joseph A,Zitnik R,Wines J,Kelley M,Rockwell S

    更新日期:1994-06-01 00:00:00

  • Antibiotic use and risk of non-Hodgkin lymphomas.

    abstract::Clinical case reports have suggested that specific bacterial infections are associated with certain non-Hodgkin lymphoma (NHL) subtypes. Epidemiological case-control studies have been conducted using antibiotics as a proxy for bacterial infections, but with inconclusive results. The aim of this study was, in a cohort ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rasmussen ML,Hjalgrim H,Mølgaard-Nielsen D,Wohlfahrt J,Melbye M

    更新日期:2012-10-01 00:00:00

  • Alpha-smooth-muscle actin and desmin expressions in human neuroblastoma cell lines.

    abstract::The neural crest gives rise to a variety of tissues, including peripheral neurons, Schwann cells, melanocytes and ectomesenchymal cells, which include the smooth-muscle cells of large arteries. Cell lines derived from neuroblastoma (a neural-crest tumor) exhibit at least 2 distinct morphological cell types, a neurobla...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sugimoto T,Ueyama H,Hosoi H,Inazawa J,Kato T,Kemshead JT,Reynolds CP,Gown AM,Mine H,Sawada T

    更新日期:1991-05-10 00:00:00

  • In vitro and in vivo antitumor effect of 2-methoxyestradiol on human melanoma.

    abstract::2-methoxyestradiol (2ME(2)) is an endogenous metabolite of estradiol with estrogen-receptor-independent antitumor and antiangiogenic activity. We examined the effects of 2ME(2) on the cellular proliferation of 8 human melanoma cell lines. We show that 2ME(2) inhibited cell proliferation by inducing apoptosis and an ar...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Dobos J,Tímár J,Bocsi J,Burián Z,Nagy K,Barna G,Peták I,Ladányi A

    更新日期:2004-12-10 00:00:00

  • Variations in sex hormone metabolism genes, postmenopausal hormone therapy and risk of endometrial cancer.

    abstract::We investigated whether variants in sex steroid hormone metabolism genes modify the effect of hormone therapy (HT) on endometrial cancer risk in postmenopausal non-Hispanic white women. A nested case-control study was conducted within the California Teachers Study (CTS). We genotyped htSNPs in six genes involved in th...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Razavi P,Lee E,Bernstein L,Van Den Berg D,Horn-Ross PL,Ursin G

    更新日期:2012-04-01 00:00:00

  • Clinicopathological characteristics of p53 overexpression in endometrial cancers.

    abstract::Using immunohistochemical methods, we analyzed the association between nuclear p53 overexpression and various clinicopathological parameters in patients with endometrial cancers. Formalin-fixed and paraffin-embedded tissue sections from 139 cases of endometrial cancer (endometrioid type, 126; serous papillary type, 12...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Inoue M,Okayama A,Fujita M,Enomoto T,Sakata M,Tanizawa O,Ueshima H

    更新日期:1994-07-01 00:00:00

  • Radiosensitivity of small-cell lung cancer xenografts compared with activity of c-myc, N-myc, L-myc, c-raf-1 and K-ras proto-oncogenes.

    abstract::Oncogenes of the myc family c-raf-1 and K-ras have been reported to modulate radiosensitivity. We examined the possible relationship between in vivo radiosensitivity to single-dose irradiation with 3-10 Gy, and activity of these proto-oncogenes in 2 sets of small-cell lung cancer (SCLC) xenografts, the CPH and the GLC...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rygaard K,Slebos RJ,Spang-Thomsen M

    更新日期:1991-09-09 00:00:00

  • Occupational exposure to polyvinyl chloride as a risk factor for testicular cancer evaluated in a case-control study.

    abstract::Occupational exposures were assessed in a case-control study on testicular cancer using self-administered questionnaires. In total, answers were obtained for 148 (91%) cases and 315 (87%) controls. Of the cases, 101 had seminoma and 47 had embryonal testicular cancer. An increased odds ratio (OR) was found for exposur...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hardell L,Ohlson CG,Fredrikson M

    更新日期:1997-12-10 00:00:00

  • Single nucleotide polymorphisms in the matrix metalloproteinase gene family and the frequency and duration of gastroesophageal reflux disease influence the risk of esophageal adenocarcinoma.

    abstract::The matrix metalloproteinase (MMP) family of proteins mediates various cellular pathways, including apoptosis and angiogenesis. Polymorphisms of MMP genes are associated with increased esophageal adenocarcinoma (EAC) risk. Gastroesophageal reflux disease (GERD) is an established EAC risk factor. We examined whether MM...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Cheung WY,Zhai R,Bradbury P,Hopkins J,Kulke MH,Heist RS,Asomaning K,Ma C,Xu W,Wang Z,Hooshmand S,Su L,Christiani DC,Liu G

    更新日期:2012-12-01 00:00:00

  • Silencing of APAF-1 in B-CLL results in poor prognosis in the case of concomitant p53 mutation.

    abstract::Apoptosis protease-activating factor 1 (APAF-1), a transcriptional target of p53, is a cytosolic adaptor protein that links the mitochondrial apoptosis pathway to the caspase cascade. Here, we aimed to study the impact of APAF-1 expression levels on cell death induced by anticancer drugs or ionizing irradiation (IR) a...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sturm I,Bosanquet AG,Radetzki S,Hummel M,Dörken B,Daniel PT

    更新日期:2006-05-01 00:00:00

  • Clinical significance of CXCR3 and CXCR4 expression in primary melanoma.

    abstract::Tumor cell migration involved in metastases is a tightly regulated, nonrandom process. Chemokines have been identified as critical molecules guiding cell migration. We performed a prospective study to analyze a possible association between the expression of chemokine receptors CXCR3 and CXCR4 by primary melanoma and c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Longo-Imedio MI,Longo N,Treviño I,Lázaro P,Sánchez-Mateos P

    更新日期:2005-12-10 00:00:00

  • SV-IV, a major protein secreted from rat seminal vesicle epithelium, promotes lymphocyte cytotoxic activity against the lymphoblastoid Raji cell line in human peripheral blood mononuclear cells.

    abstract::The treatment of human peripheral blood mononuclear cells (PBMC) with micromolar concentrations of SV-IV, a major protein secreted from the rat seminal vesicle epithelium, promotes in this cell population a marked cytotoxic activity against the Raji lymphoblastoid cell line. This activity is apparently due to cell-to-...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Peluso G,Marchese M,Furgi A,Ranieri M,Russo Spena S,Ravagnan G,Fuggetta MP,Porta R,Metafora V,Metafora S

    更新日期:1997-07-17 00:00:00

  • Embryo-derived teratocarcinoma. IV. The role of immune factors in the regulation of teratocarcinogenesis.

    abstract::Seven-day-old mouse embryos were transplanted under the kidney capsule of adult syngeneic recipients which were either X-irradiated, treated with cyclophosphamide, splenectomized or immunized with teratocarcinoma cell lines prior to embryonic transplantation. The yield of malignant tumors was decreased in preirradiate...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Damjanov I,Bagasra O,Dominis M,Solter D

    更新日期:1982-12-15 00:00:00

  • Ca2+ and calmodulin are involved in the processes conferring stability to DNA in proliferating neoplastic cells.

    abstract::We have examined, in proliferating neoplastic cells, the effect on DNA of EGTA (a chelator of Ca2+) and W7 (an inhibitor of calmodulin). The treatment results in release of single-stranded DNA fragments (2-10 kb) from pre-labelled HMW DNA. When DNA from synchronized neoplastic cells in S phase is examined, almost all ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lönn U,Lönn S

    更新日期:1986-06-15 00:00:00

  • DNA base modifications in renal chromatin of Wistar rats treated with a renal carcinogen, ferric nitrilotriacetate.

    abstract::Ferric nitrilotriacetate (Fe-NTA) causes renal proximal tubular necrosis, a consequence of iron ion-mediated free-radical-associated damage, that finally leads to a high incidence of renal adenocarcinoma in male rats and mice. We have investigated the levels of typical hydroxyl radical-induced DNA base modifications i...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Toyokuni S,Mori T,Dizdaroglu M

    更新日期:1994-04-01 00:00:00

  • Niacin intake and risk of skin cancer in US women and men.

    abstract::A recent clinical trial found a protective role of niacinamide, a derivative of niacin, against skin cancer recurrence. However, there is no epidemiologic study to assess the association between niacin intake and risk of skin cancer [basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and melanoma]. We prospecti...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Park SM,Li T,Wu S,Li WQ,Weinstock M,Qureshi AA,Cho E

    更新日期:2017-05-01 00:00:00

  • Nanomedicine in management of hepatocellular carcinoma: Challenges and opportunities.

    abstract::Hepatocellular carcinoma is the second leading cause of cancer deaths worldwide. It is characterized by unique features that can be utilized for selective and targeted therapy, which aids in preserving healthy tissues from deteriorating effects of traditional chemotherapeutics. In this minireview, a brief overview of ...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Mohamed NK,Hamad MA,Hafez MZ,Wooley KL,Elsabahy M

    更新日期:2017-04-01 00:00:00

  • Lifestyle factors, autoimmune disease and family history in prognosis of non-hodgkin lymphoma overall and subtypes.

    abstract::Lifestyle factors and medical history are known to influence risk of non-Hodgkin lymphoma (NHL). Whether these factors affect the prognosis of NHL, especially its subtypes, is unclear. To investigate this, the association between these factors and all-cause and lymphoma-related mortality was assessed in a population-b...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Simard JF,Baecklund F,Chang ET,Baecklund E,Hjalgrim H,-Olov Adami H,Glimelius B,Smedby KE

    更新日期:2013-06-01 00:00:00

  • The neu-oncogene protein as a predictive factor for haematogenous metastases in breast cancer patients.

    abstract::In a prospective study with a median follow-up of 13 months on a series of 71 breast cancer patients, 9 developed haematogenous metastases. The neu protein was found on the cell membranes in 27 of the 71 carcinomas (38%) by an immunohistochemical technique using a monoclonal antibody (MAb). Eight of the 9 patients wit...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: De Potter CR,Beghin C,Makar AP,Vandekerckhove D,Roels HJ

    更新日期:1990-01-15 00:00:00

  • Suppression of Friend leukemia virus by Bacillus Calmette-Guérin and a streptococcal preparation, OK-432.

    abstract::OK-432, a streptococcal preparation, and BCG effectively inhibited splenomegaly in Friend leukemia virus (FLV)-infected mice. Divided drug dosage resulted in stronger inhibition than single administration. When the second dose was fixed at the 3rd pre-infection day, the best timing for the first dose was approximately...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tsuruo T,Iida H,Tsukagoshi S,Sakurai Y

    更新日期:1980-01-15 00:00:00

  • Gamma-glutamyltranspeptidase expression regulates the growth-inhibitory activity of the anti-tumor prodrug gamma-L-glutaminyl-4-hydroxy-3-iodobenzene.

    abstract::gamma-L-glutaminyl-4-hydroxy-3-iodobenzene (I-GHB), a novel iodinated analog of gamma-L-glutaminyl-4-hydroxybenzene (GHB), demonstrates greater anti-tumor activity in human and in murine melanoma cell lines. These phenolic amides are substrates for gamma-glutamyltranspeptidase (GGTP; E.C., a cell-membrane-ass...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Prezioso JA,Hughey RP,Wang N,Damodaran KM,Bloomer WD

    更新日期:1994-03-15 00:00:00