Abstract:
:It has been demonstrated that the chemopreventive agent N-(4-hydroxyphenyl)retinamide (4-HPR) induces apoptotic cell death, but recent data has suggested that late stage/recurrent tumours lose their response to 4-HPR-induced cell death by mechanisms that are unknown. Our study investigated the ability of 4-HPR to induce cell death in keratinocyte cell lines that represent different stages of carcinogenesis and the role of TGF-beta signalling in the induction of cell death by 4-HPR. We show that treatment of the immortalised keratinocyte cell line HaCaT with 10(-5) M 4-HPR induced cell death by apoptosis and caused an accumulation of cells in the G0/G1 phase of the cell cycle. Using a genetically related series of human skin keratinocytes derived from HaCaT that reflect tumour progression and metastasis in vivo, we demonstrate that 4-HPR-induced cell death and apoptosis is attenuated in the more aggressive tumour cell lines but that a reduced level of response is retained. Response to TGF-beta-induced growth inhibition was also reduced in the more aggressive cell lines. Treatment of HaCaT cells with 4-HPR induced TGF-beta2 expression and an increase in the amount of active TGF-beta in the culture medium. The inhibition of TGF-beta signalling attenuated 4-HPR-induced apoptosis and both TGF-beta1 and TGF-beta2 potentiated 4-HPR-induced apoptosis and enhanced 4-HPR-induced growth inhibition. Our results demonstrate that loss of response to 4-HPR correlates with a loss of response to the growth inhibitory effects of TGF-beta and that adjuvant therapies that upregulate TGF-beta may enhance the chemopreventive effects of 4-HPR.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Davies M,Paterson IC,Ganapathy A,Prime SSdoi
10.1002/ijc.22263subject
Has Abstractpub_date
2006-12-15 00:00:00pages
2803-11issue
12eissn
0020-7136issn
1097-0215journal_volume
119pub_type
杂志文章abstract::In order to define technical limitations of conventional external beam irradiation for clinically localized prostate cancer, we evaluated the impact of several reduced-field treatment factors, such as reduced-field (RF) irradiated volume, RF technique, photon energy of treatment, and dose on survival endpoints and loc...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-10-20 00:00:00
abstract::Lymphoma EL-4 of B6 origin was inoculated into lethally-irradiated syngeneic B6 or resistant BD2F1 hybrid recipients. Marked impairment of lymphoma cell proliferation (i.e. hybrid resistance, HR) occurred in the spleen but not in the liver or lung of BD2F1 hosts. Treatment with DTIC at the optimal dose of 80 mg/kg i.p...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280416
更新日期:1981-10-15 00:00:00
abstract::Early detection, clinical management and disease recurrence monitoring are critical areas in cancer treatment in which specific biomarker panels are likely to be very important in each of these key areas. We have previously demonstrated that levels of alpha-2-heremans-schmid-glycoprotein (AHSG), complement component C...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26462
更新日期:2012-08-15 00:00:00
abstract::Cell lines derived from 13 different human colon adenocarcinomas were examined for production of fibronectin by ELISA and for cell-surface expression of fibronectin by indirect immunofluorescence. Two squamous epithelial cell lines obtained from tumors of the upper aerodigestive tract were used as controls. None of th...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910470319
更新日期:1991-02-01 00:00:00
abstract::A challenging task of the immune system is to fight cancer cells. However, a variety of human cancers educate immune cells to become tumor supportive. This is exemplified for tumor-associated macrophages (TAMs), which are polarized towards an anti-inflammatory and cancer promoting phenotype. Mechanistic explanations, ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24594
更新日期:2009-11-01 00:00:00
abstract::T24 human bladder carcinoma cells reveal a high locomotor activity (70% locomoting cells) within a 3-dimensional collagen matrix. This high migratory activity is induced by an autocrine engagement of the interleukin-8 receptor A, as was shown by antibodies neutralizing the secreted interleukin-8. Treatment of the cell...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10424
更新日期:2002-06-10 00:00:00
abstract::Three monoclonal antibodies (MAbs) against p53 protein (PAb 24o, DO-I and PAb1801) were used to define the immunophenotype of 13 melanoma cell lines. Immunoreactions could be detected in 12 out of 13 cell lines by using the indirect immunofluorescence technique. In 7 of these the majority of cells displayed cytoplasmi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910540427
更新日期:1993-06-19 00:00:00
abstract::Gefitinib has shown meaningful antitumor activity with tolerable toxicity in chemotherapy-refractory NSCLC in previous studies. Moreover, EGFR expression failed to show a correlation with response. In an attempt to identify predictive markers of response, we have investigated the tumoral expression of key signaling mo...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1002/ijc.20550
更新日期:2005-01-01 00:00:00
abstract::The relative expression of estrogen receptor (ER) and progesterone receptor (PR) mRNA transcripts was measured in 71 primary breast-cancer biopsies. ER and PR binding activity were estimated in parallel by the dextran-coated-charcoal method. There was a close correlation between the amount of ER mRNA and estradiol bin...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910590310
更新日期:1994-11-01 00:00:00
abstract::The expression of the recently described rap genes, closely related in the effector region to the ras proto-oncogenes, was examined by Northern blot analysis in 41 primary human tumors. The structural and in vitro biological properties of the rap gene products suggest their possible antagonistic action in the same eff...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440608
更新日期:1989-12-15 00:00:00
abstract::The expression patterns of glycolipids of human renal-cell carcinoma were studied in primary tumors from 23 cases and 5 metastatic lesions from 4 cases using HPTLC (high-performance thin-layer chromatography). The expression pattern of glycolipids in primary tumors was characteristic of the histological cell type. In ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910490303
更新日期:1991-09-30 00:00:00
abstract::The loss of E-cadherin expression and the translocation of beta-catenin to the nucleus are frequently associated with the metastatic conversion of epithelial cells. In the nucleus, beta-catenin binds to the TCF/LEF-1 (T-cell factor/ lymphoid enhancer factor) transcription factor family resulting in the activation of s...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21291
更新日期:2006-01-01 00:00:00
abstract::Others have argued that as many as a third of women treated for high-grade cervical intraepithelial neoplasia (CIN) would have developed cervical cancer in the absence of screening and treatment. Under various assumptions and using past data on CIN grade 3 (CIN3) registrations in England and Scotland, we estimate what...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23922
更新日期:2009-01-15 00:00:00
abstract::It is well known that tumors arising from tissues such as kidney, pancreas, liver and stomach are particularly refractory to treatment. Searching for new anticancer drugs using cells in culture has yielded some effective therapies, but these refractory tumors remain intractable. Studies that compare cells grown in sus...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.20671
更新日期:2005-03-01 00:00:00
abstract::We have previously identified a region containing 16 CpGs within the MGMT CpG islands which is critical for the transcriptional control of MGMT (Malley, Acta Neuropathol 2011). To investigate the patterns and incidence of MGMT methylation in astrocytic and oligodendroglial tumors, we quantitatively assessed methylatio...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26499
更新日期:2012-09-01 00:00:00
abstract::Metastasis to the liver often occurs in patients during the natural course of pancreatic cancer. Using carcinoma cell lines established from 9 such patients, we examined phenotypes of cell lines to search for correlations with their potential to metastasize to the liver. Anti-asialo GMI-treated nude mice were used. PC...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960822)69:4<290::AID-IJC
更新日期:1996-08-22 00:00:00
abstract::Agouti signaling protein (ASIP) locus on chromosome 20q11 is implicated, as shown by genome-wide association studies, in phenotype variation and melanoma risk. We genotyped 837 melanoma cases and 1,154 controls for 21 single nucleotide polymorphisms (SNPs) informative for 495 polymorphisms at the locus. Our data showe...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27648
更新日期:2013-01-01 00:00:00
abstract::Posttransplant lymphoproliferative disorder (PTLD) is a common and life-threatening complication of immunosuppression used to prevent rejection of solid organ and bone marrow transplants. There is no standardized treatment algorithm, but numerous management strategies are available. We describe a patient who developed...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000420)90:2<104::aid-ijc
更新日期:2000-04-20 00:00:00
abstract::To test whether alloimmunization with H-2 or/and non-H-2 different normal tissues may increase the immunity to syngeneic tumors, groups of BALB/c (H-2d) mice were immunized with a series of allogeneic lymphoid cells and then challenged i.p. with syngeneic lymphoma cells. The outgrowth of otherwise lethal doses of the ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910290316
更新日期:1982-03-15 00:00:00
abstract::A population-based case-control study was conducted to evaluate risk of developing multiple myeloma (MM) associated with personal history of autoimmune diseases and occurrence of autoimmune and selected hematologic disorders in first-degree relatives. Data were obtained for all (n = 8,406) MM cases diagnosed in Sweden...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21745
更新日期:2006-06-15 00:00:00
abstract::Our aim was to evaluate whether serum concentrations of squamous cell carcinoma antigen (SCC-Ag) are an independent prognostic factor in patients with vulvar cancer. We measured SCC-Ag in pretreatment serum samples of 55 patients with squamous cell vulvar cancer, 30 patients with vulvar intraepithelial neoplasia (VIN)...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990621)84:3<299::aid-ijc
更新日期:1999-06-21 00:00:00
abstract::Several pathways have been implicated in the pathogenesis of endometrial carcinoma. Based on recent reports, BRAF mutations provide an alternative route for activation of the RAS signalling pathway. The CDKN2A (p16) tumour suppressor gene is also altered in several tumour types. We therefore wanted to assess the patte...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20702
更新日期:2005-07-20 00:00:00
abstract::By using a functional complementation approach, suppression of tumorigenicity was observed after transfer of intact or truncated copies of chromosome 3 into a nasopharyngeal carcinoma (NPC) HONE1 cell line. The extra exogenous chromosome 3 in the microcell hybrids (MCHs) significantly extended the lag period of tumor ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23528
更新日期:2008-07-15 00:00:00
abstract::Although some authorities consider smoking to be an established risk factor for colorectal cancer, the international literature is not entirely consistent. Further, only 1 study has addressed the association with smokeless tobacco and none with Scandinavian moist snuff (snus). This retrospective cohort study included ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25305
更新日期:2011-01-01 00:00:00
abstract::An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times. The PANC-1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility of B type. Chromosome studies show a modal numb...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910150505
更新日期:1975-05-15 00:00:00
abstract::To evaluate renal pelvis and ureter (RPU) cancer risk in relation to lifetime use of analgesics, a population-based case-control study was carried out in 3 areas of the United States. Among 502 cases and 496 controls diagnosed and interviewed during 1983-1986, no significant increases in risk were found for any of the...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.2910620105
更新日期:1995-07-04 00:00:00
abstract::Cetuximab is a human/mouse chimeric IgG1 monoclonal antibody (mAb) to epidermal growth factor receptor, approved for colorectal carcinoma treatment in combination with chemotherapy. The immune-mediated effects elicited by its human fraction of crystallization moiety might critically contribute to the overall anti-tumo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26181
更新日期:2012-04-01 00:00:00
abstract::Chemokine receptors are known to regulate homing of lymphocytes into secondary lymphoid organs and may also be involved in the lymphatic spread of solid tumors. Therefore, the assessment of chemokine receptor expression on colorectal carcinomas could potentially improve the prediction of lymph node spread. This is of ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21123
更新日期:2005-09-20 00:00:00
abstract::There is growing evidence suggesting that cytotoxic activity of cisplatin is closely associated with increased generation of reactive oxygen species (ROS). Therefore, this study was undertaken to examine oxidative DNA damage, which arises as a result of chemotherapy with cisplatin. Using HPLC prepurification/isotope d...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22088
更新日期:2006-11-01 00:00:00
abstract::Ornithine decarboxylase may be a useful biomarker for risk of neoplasia in colorectal tissues. Investigators have reported enzyme activities varying by as much as 10- to 20-fold using variations of the usual 14CO2 release assay. We have examined the effect of different methodologic factors on calculated ornithine deca...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910520305
更新日期:1992-09-30 00:00:00