Abstract:
:To identify genes involved in stress resistance and heat hardening, replicate lines of Drosophila melanogaster were selected for increased resistance to knockdown by a 39 degrees heat stress. Two selective regimes were used, one with and one without prior hardening. Mean knockdown times were increased from approximately 5 min to > 20 min after 18 generations. Initial realized heritabilities were as high as 10% for lines selected without hardening, and crosses between lines indicated simple additive gene effects for the selected phenotypes. To survey allelic variation and correlated selection responses in two candidate stress genes, hsr-omega and hsp68, we applied denaturing gradient gel electrophoresis to amplified DNA sequences from small regions of these genes. After eight generations of selection, allele frequencies at both loci showed correlated responses for selection following hardening, but not without hardening. The hardening process itself was associated with a hsp68 frequency change in the opposite direction to that associated with selection that followed hardening. These stress loci are closely linked on chromosome III, and the hardening selection established a disequilibrium, suggesting an epistatic effect on resistance. The data indicate that molecular variation in both hsr-omega and.hsp68 contribute to natural heritable variation for hardened heat resistance.
journal_name
Geneticsjournal_title
Geneticsauthors
McColl G,Hoffmann AA,McKechnie SWsubject
Has Abstractpub_date
1996-08-01 00:00:00pages
1615-27issue
4eissn
0016-6731issn
1943-2631journal_volume
143pub_type
杂志文章相关文献
GENETICS文献大全abstract::Currently used tests of Hardy-Weinberg proportions do not take into account the nature of the alternative hypothesis, which is generally a heterozygote deficiency. Different exact tests, appropriate for small sample size and large number of alleles, are proposed in this perspective, and their properties are evaluated ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Mutants of Paramecium caudatum with abnormal swimming behavior or responses to cations were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Some of the mutants, like pawn in P. tetraurelia, cannot swim backward and are called CNR. Seven independently obtained CNR mutants belonged to three complement...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::Several human pathogens (e.g., Bacillus anthracis, Yersinia pestis, Bordetella pertussis, Plasmodium falciparum, and Mycobacterium tuberculosis) have very restricted unselected allelic variation in structural genes, which hinders study of the genetic relationships among strains and strain-trait correlations. To addres...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::Laboratory strains of mice are thought to be derived from wild populations of Mus domesticus. Many instances of non-domesticus genetic information fixed in these strains have been described, however, and the amount of strain-to-strain genetic variation exceeds that found in wild domesticus populations. In order to est...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::At pH 6.8, a substantial fraction of recombinant colonies obtained from conjugation with an HfrH donor contained multiple recombinant classes in a single colony (polygenotype colony). In contrast, when the conjugation was performed at pH 7.6, the number of polygenotypic colonies was drastically reduced, and the recomb...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract::The small subunit ribosomal RNA gene (srDNA) has been used extensively for phylogenetic analyses. One common assumption in these analyses is that substitution rates are biased toward transitions. We have developed a simple method for estimating relative rates of base change that does not assume rate constancy and take...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
abstract::Sixteen inbred or partially inbred strains of rabbits were investigated for electrophoretic and quantitative variations of alpha-1-antitrypsin (A-1-AT). We found interindividual differences in the electrophoretic A-1-AT patterns as well as quantitative differences in the concentrations of A-1-AT and the serum trypsin-...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::Trans-species polymorphism, meaning the presence of alleles in different species that are more similar to each other than they are to alleles in the same species, has been found at loci associated with vegetative incompatibility in filamentous fungi. If individuals differ at one or more of these loci (termed het for h...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::We have selected 210 mutants able to grow on sucrose in the presence of 2-deoxyglucose. We identified recessive mutations in three major complementation groups that cause constitutive (glucose-insensitive) secreted invertase synthesis. Two groups comprise alleles of the previously identified HXK2 and REG1 genes, and t...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-02-01 00:00:00
abstract::The availability of genomewide dense markers brings opportunities and challenges to breeding programs. An important question concerns the ways in which dense markers and pedigrees, together with phenotypic records, should be used to arrive at predictions of genetic values for complex traits. If a large number of marke...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.101501
更新日期:2009-05-01 00:00:00
abstract::The neurogenic loci comprise a small group of genes which are required for proper division between the neural and epidermal pathways of differentiation within the neuroectoderm. Loss of neurogenic gene function results in the misrouting of prospective epidermal cells into neuroblasts. A molecular analysis of the neuro...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract::The distribution of the number of HLA haplotypes shared by sibs affected with the same HLA-linked disease can be used to obtain information on the genetics of the disease. Since the inception of the use of sib-pair methods for the analysis of the HLA-associated diseases, the question has been raised of how to include ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::In the present study, we investigated intrachromosomal homologous recombination in a murine hybridoma in which the recipient for recombination, the haploid, endogenous chromosomal immunoglobulin mu-gene bearing a mutation in the constant (Cmu) region, was separated from the integrated single copy wild-type donor Cmu r...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::The challenges of breeding autotetraploid potato (Solanum tuberosum) have motivated the development of alternative breeding strategies. A common approach is to obtain uniparental dihaploids from a tetraploid of interest through pollination with S. tuberosum Andigenum Group (formerly S. phureja) cultivars. The mechanis...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302843
更新日期:2020-02-01 00:00:00
abstract::Inverted DNA repeats are an at-risk motif for genetic instability that can induce both deletions and recombination in yeast. We investigated the role of the length of inverted repeats and size of the DNA separating the repeats for deletion and recombination. Stimulation of both deletion and recombination was directly ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Mating type interconversion in Saccharomyces cerevisiae occurs by transposition of copies of the a or alpha mating type cassettes from inactive loci, HML and HMR, to an active locus, MAT. The lack of expression of the a and alpha genes at the silent loci results from repression by trans-acting regulators encoded by SI...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-05-01 00:00:00
abstract::A newly characterized Drosophila melanogaster gene, logjam (loj), functions in female reproduction by modulating oviposition behavior. The locus encodes at least six overlapping transcripts with unique 5' ends. P-element mutants that express very low levels of loj transcripts are unable to oviposit mature eggs. This p...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::Previous studies on gene expression in aneuploids revealed numerous trans-acting dosage effects. Segmental aneuploidy of each varied chromosomal region exhibited predominantly inverse effects on several target genes. Here, dosage regulation was examined in a maize (Zea mays L.) ploidy series where the complete genomic...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::A model system for studying double-strand-break (DSB)-induced genetic recombination in vivo based on the ets1 segCDelta strain of bacteriophage T4 was developed. The ets1, a 66-bp DNA fragment of phage T2L containing the cleavage site for the T4 SegC site-specific endonuclease, was inserted into the proximal part of t...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::Heritable silencing effects are gene suppression phenomena that can persist for generations after induction. In the majority of RNAi experiments conducted in Caenorhabditis elegans, the silencing response results in a hypomorphic phenotype where the effects recede after the F1 generation. F2 and subsequent generations...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.089433
更新日期:2008-11-01 00:00:00
abstract::A leucine, leucyl-tRNA synthetase-dependent pathway activates TorC1 kinase and its downstream stimulation of protein synthesis, a major nitrogen consumer. We previously demonstrated, however, that control of Gln3, a transcription activator of catabolic genes whose products generate the nitrogenous precursors for prote...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.173831
更新日期:2015-02-01 00:00:00
abstract::An analysis of the effects of spontaneous mutations affecting age-specific mortality was conducted using 29 lines of Drosophila melanogaster that had accumulated spontaneous mutations for 19 generations. Divergence among the lines was used to estimate the mutational variance for weekly mortality rates and the covarian...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::We previously isolated a pollen factor, ui6.1, which encodes a Cullin1 protein (CUL1) that functions in unilateral interspecific incompatibility (UI) in Solanum. Here we show that CUL1 is also required for pollen function in self-incompatibility (SI). We used RNA interference (RNAi) to reduce CUL1 expression in pollen...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.158279
更新日期:2014-02-01 00:00:00
abstract::Next generation sequencing (NGS) is about to revolutionize genetic analysis. Currently NGS techniques are mainly used to sequence individual genomes. Due to the high sequence coverage required, the costs for population-scale analyses are still too high to allow an extension to nonmodel organisms. Here, we show that NG...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.114397
更新日期:2010-09-01 00:00:00
abstract::Heterogeneous genetic divergence can accumulate across the genome when populations adapt to different habitats while still exchanging alleles. How long does diversification take and how much of the genome is affected? When divergence occurs in parallel from standing genetic variation, how often are the same haplotypes...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300610
更新日期:2018-07-01 00:00:00
abstract::In eukaryotic cells, checkpoint pathways arrest cell-cycle progression if a particular event has failed to complete appropriately or if an important intracellular structure is defective or damaged. Saccharomyces cerevisiae strains that lack the SFP1 gene fail to arrest at the G2 DNA-damage checkpoint in response to ge...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Trichome development in Arabidopsis thaliana is a well-characterized model for the study of plant cell differentiation. Two genes that play an essential role in the initiation of trichome development are GL1 and TTG. Mutations in either gene prevent the initiation of most trichomes. The GL1 gene encodes a myb-related ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::The nuclear aod-1 gene of Neurospora crassa encodes the alternative oxidase and is induced when the standard cytochrome-mediated respiratory chain of mitochondria is inhibited. To study elements of the pathway responsible for alternative oxidase induction, we generated a series of mutations in the region upstream from...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.068635
更新日期:2007-04-01 00:00:00
abstract::Fifty-two wee mutants that undergo mitosis and cell division at a reduced size compared with wild type have been genetically analyzed. The mutants define two genes, wee1 and cdc2, which control the timing of mitosis. Fifty-one of the mutants map at the wee1 locus, which is unlinked to any known cdc gene. One of the we...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::To understand gene function, the cre/loxP conditional system is the most powerful available for temporal and spatial control of expression in mouse. However, the research community requires more cre recombinase expressing transgenic mouse strains (cre-drivers) that restrict expression to specific cell types. To addres...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.301984
更新日期:2019-04-01 00:00:00