Abstract:
:A model system for studying double-strand-break (DSB)-induced genetic recombination in vivo based on the ets1 segCDelta strain of bacteriophage T4 was developed. The ets1, a 66-bp DNA fragment of phage T2L containing the cleavage site for the T4 SegC site-specific endonuclease, was inserted into the proximal part of the T4 rIIB gene. Under segC(+) conditions, the ets1 behaves as a recombination hotspot. Crosses of the ets1 against rII markers located to the left and to the right of ets1 gave similar results, thus demonstrating the equal and symmetrical initiation of recombination by either part of the broken chromosome. Frequency/distance relationships were studied in a series of two- and three-factor crosses with other rIIB and rIIA mutants (all segC(+)) separated from ets1 by 12-2100 bp. The observed relationships were readily interpretable in terms of the modified splice/patch coupling model. The advantages of this localized or focused recombination over that distributed along the chromosome, as a model for studying the recombination-replication pathway in T4 in vivo, are discussed.
journal_name
Geneticsjournal_title
Geneticsauthors
Shcherbakov V,Granovsky I,Plugina L,Shcherbakova T,Sizova S,Pyatkov K,Shlyapnikov M,Shubina Okeywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
543-56issue
2eissn
0016-6731issn
1943-2631journal_volume
162pub_type
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